scholarly journals Oligoasthenoteratospermia and sperm tail bending in PPP4C-deficient mice

2020 ◽  
Author(s):  
F Han ◽  
M Z Dong ◽  
W L Lei ◽  
Z L Xu ◽  
F Gao ◽  
...  
Keyword(s):  
Mouse Model ◽  
Male Infertility ◽  
Sperm Motility ◽  
Protein Phosphatase ◽  
Sperm Count ◽  
Physiological Role ◽  
Nuclear Condensation ◽  
Sperm Tail ◽  
Structure Assembly ◽  
Induced Defects

Abstract Protein phosphatase 4 (PPP4) is a protein phosphatase that, although highly expressed in the testis, currently has an unclear physiological role in this tissue. Here, we show that deletion of PPP4 catalytic subunit gene Ppp4c in the mouse causes male-specific infertility. Loss of PPP4C, when assessed by light microscopy, did not obviously affect many aspects of the morphology of spermatogenesis, including acrosome formation, nuclear condensation and elongation, mitochondrial sheaths arrangement and “9 + 2” flagellar structure assembly. However, the PPP4C mutant had sperm tail bending defects (head-bent-back), low sperm count, poor sperm motility and had cytoplasmic remnants attached to the middle-piece of the tail. The cytoplasmic remnants were further investigated by transmission electron microscopy to reveal that a defect in cytoplasm removal appeared to play a significant role in the observed spermiogenesis failure and resulting male infertility. A lack of PPP4 during spermatogenesis causes defects that are reminiscent of oligoasthenoteratospermia (OAT), which is a common cause of male infertility in humans. Like the lack of functional PPP4 in the mouse model, OAT is characterized by abnormal sperm morphology, low sperm count and poor sperm motility. Although the causes of OAT are probably heterogeneous, including mutation of various genes and environmentally induced defects, the detailed molecular mechanism(s) has remained unclear. Our discovery that the PPP4C-deficient mouse model shares features with human OAT might offer a useful model for further studies of this currently poorly understood disorder.

scholarly journals Prevalence and Patterns of Male Infertility Based on Semen Analysis in Patients Visiting Massawa Hospital; Eritrea: Cross-Sectional Study; 2020

2021 ◽  
Author(s):  
Berhe Tesfai ◽  
Fitsum Kibreab ◽  
Hailemichael Gebremariam ◽  
Liwam Abraham
Keyword(s):  
Male Infertility ◽  
Sperm Motility ◽  
Sperm Count ◽  
Semen Analysis ◽  
Male Factor Infertility ◽  
Male Factor ◽  
Cross Sectional ◽  
Semen Volume ◽  
Primary Type ◽  
Study Participants

Abstract Background Semen analysis is the first step to identify male factor infertility. World Health Organization estimates that male factor accounts for 50% of couple sub-fertility. The objective of this study was to determine the prevalence and patterns of male factor infertility based on semen analysis in patients visiting Massawa Hospital with infertility complaints. Methods It was a retrospective, descriptive cross sectional hospital based type of study with a census sampling method. Patient’s medical records and hospital laboratory register were used to retrieve semen analysis results of patients from June 2018 to June 2020. Ethical approval was obtained from the Ministry of Health Research and Ethical Review Committee. Confidentiality of patients records kept was secured and consent was obtained from study participants to use their data. Results were presented in frequency, tables and p value < 0.05 was considered significant. Results A total of 112 patients data was analyzed in the study with 49.1% were aged between 20 to 30 years. The prevalence of male factor infertility in these patients was found to be 42% and 79.5% of them had primary type of infertility. Of the study participants; 63 (56.3%), 72 (64.3%) and 70 (62.5%) had sperm count < 15 million sperms/ml, sperm motility < 40 % and morphology of < 60% respectively. One tenth, 15 (13.4%) of the patients had a semen volume of < 1.5ml/ejaculate, out of which 13(86.7%) had primary type of infertility. Moreover; 72 (64.3%) patients had total sperm count/ejaculate of < 39 million and 59 (82%) of these had primary type of infertility. In addition; 50.8% and 50% of patients aged 20 to 30 years had a sperm count < 15 million/ml and sperm motility of < 40% respectively. Conclusion The prevalence of male factor infertility was slightly higher and was dominated with primary infertility. Most patients had lower sperm count, sperm motility but higher semen volume, and majority of the patients with abnormal sperm results were aged between 20 to 30 years. Further prospective researches to determine the risk factors for male infertility and introducing assisted type of fertility in Eritrea are highly recommended.

scholarly journals Coenzyme Q10, oxidative stress, and male infertility: A review

2021 ◽  
Vol 48 (2) ◽  
pp. 97-104
Author(s):  
Ahmed T. Alahmar ◽  
Aldo E. Calogero ◽  
Rajender Singh ◽  
Rossella Cannarella ◽  
Pallav Sengupta ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Male Infertility ◽  
Coenzyme Q10 ◽  
Semen Quality ◽  
Sperm Count ◽  
Specific Treatment ◽  
Physiological Role ◽  
Semen Parameters ◽  
Sperm Dna Fragmentation ◽  
Male Reproductive Function

Male infertility has a complex etiopathology, which mostly remains elusive. Although research has claimed that oxidative stress (OS) is the most likely underlying mechanism of idiopathic male infertility, the specific treatment of OS-mediated male infertility requires further investigation. Coenzyme Q10 (CoQ10), a vitamin-like substance, has been found in measurable levels in human semen. It exhibits essential metabolic and antioxidant functions, as well as playing a vital role in mitochondrial bioenergetics. Thus, CoQ10 may be a key player in the maintenance of biological redox balance. CoQ10 concentrations in seminal plasma directly correlate with semen parameters, especially sperm count and sperm motility. Seminal CoQ10 concentrations have been shown to be altered in various male infertility states, such as varicocele, asthenozoospermia, and medical or surgical regimens used to treat male infertility. These observations imply that CoQ10 plays an important physiological role in the maintenance and amelioration of semen quality. The present article thereby aimed to review the possible mechanisms through which CoQ10 plays a role in the regulation of male reproductive function, and to concisely discuss its efficacy as an ameliorative agent in restoring semen parameters in male infertility, as well as its impact on OS markers, sperm DNA fragmentation, pregnancy, and assisted reproductive technology outcomes.

scholarly journals Influence of Lifestyle and Environmental Factors on Semen Quality in Ghanaian Men

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Richard Michael Blay ◽  
Abigail Duah Pinamang ◽  
Augustine E. Sagoe ◽  
Ewurama Dedea Ampadu Owusu ◽  
Nii Koney-Kwaku Koney ◽  
...  
Keyword(s):  
Environmental Factors ◽  
Male Infertility ◽  
Sperm Motility ◽  
Semen Quality ◽  
Sperm Quality ◽  
Sperm Count ◽  
Semen Analysis ◽  
Reproductive Age ◽  
Adult Males ◽  
Lifestyle Habits

Introduction. Male infertility is known to contribute about half of all infertility cases. In Ghana, the prevalence of male infertility is higher (15.8%) than in females (11.8%). Sperm quality is associated with the likelihood of pregnancy and known to be the cause of male fertility problems 90% of the time. Exposure to certain environmental factors reduces semen quality in men. The study examined the effects of environmental and lifestyle factors on semen quality in Ghanaian men. Materials and Methods. This was a cross-sectional study involving 80 apparent healthy adult males in their reproductive age. Participants were males referred to the laboratory (Immunology Unit of the Korle-Bu Teaching Hospital) for semen analysis test and/or culture and sensitivity. Participants were made to fill out a questionnaire which entailed selected environmental factors (accidents or trauma, exposure to chemicals, radiation, and heat) and lifestyle habits (including alcohol consumption, smoking, and whether participants sat more or less than 4 hours per day). Semen samples were then collected by masturbation into sterile containers and analysed in accordance with WHO guidance for semen analysis within 60 minutes after ejaculation and collection. Results. About 69% of participants had semen pH within the normal range compared to 15% whose pH were lower than 7.2. There was a significantly high number of immotile sperm cells ( p value = 0.017) in participants who sat for more than 4 hours as compared to those that sat for less than 4 hours in a day. Active sperm motility and viability showed significant increase ( p value = 0.002 and 0.009, respectively) in participants who kept their cell phones in their side pockets. Smoking produced a twofold decrease in sperm count as smokers had a significantly lower sperm count ( 12.28 ± 10.95 × 10 6 /ml) compared to the smoke-free ( 23.85 ± 22.14 × 10 6 /ml). For exposure to STDs, no significant differences were recorded among study groups concerning semen quality. Conclusion. Sperm quality in Ghanaian men is associated with lifestyle habits. Smoking and sitting for long hours influenced sperm motility and count, respectively. Knowledge of the factors that influence sperm quality in this geographical region can contribute to informed decisions on effective management of infertility in Ghanaian men.

scholarly journals Role of Male Partners in 100 Infertile Couples

2016 ◽  
Vol 11 (1) ◽  
pp. 50-53
Author(s):  
Mah Jabeen Ara ◽  
Sk Md Bahar Hussain ◽  
Mamun Ur Rashid
Keyword(s):  
Male Infertility ◽  
Sperm Motility ◽  
Sperm Morphology ◽  
Sperm Count ◽  
Semen Analysis ◽  
Armed Forces ◽  
Medical College ◽  
Sperm Abnormality ◽  
A Prospective Study

Introduction: Approximately 15% of couples are infertile. Male infertility plays an important role in about half of these couples. This study has been done to determine whether sperm morphology and motility do really affect sperm count or spermatogenesis and at the same time causes a problem with fertility.Objectives: The objective of this study was to evaluate a male’s inability to cause pregnancy in a fertile female.Materials and Methods: This is a prospective study of 100 diagnosed infertile and subfertile couples in the age group of 23-58 yrs old male. They were selected from 01 March 2013 to 28 Feb 2014 undergoing treatment for infertility in Dhaka CMH. On the basis of sperm count the cases were grouped as follows: Normozoospermia, oligozoospermia & Azoospermia. Semen analysis was done in all the cases and the results were described with arithmetic mean and standard deviation.Results: Normozoospermia: Mean and SD of sperm count, sperm abnormality, sperm motility are 65.98± 5.05, 24.44±1.57 and 45.5±2.94 respectively; Oligozoospermia: Mean and SD of sperm count, sperm abnormality, sperm motility are 7.74±1.23, 41.1±3.78, 14.54±2.77 respectively; Azoospermia: Mean and SD of sperm count, sperm abnormality, sperm motility are absent.Conclusion: Here data statistically showed person with normozoospermia having good sperm morphology and motility than oligozoospermia and azoospermic subjects thereby causing male infertility which is responsible for hindrance in achieving pregnancy clinically.Journal of Armed Forces Medical College Bangladesh Vol.11(1) 2015: 50-53

scholarly journals Contribution of Ayurveda in the Management of Ksheena Shukra Vikara with Special Reference to Asthenozoospermia: A Case Report

2021 ◽  
pp. 140-149
Author(s):  
Manju Mohan ◽  
Sawarkar Punam ◽  
Sawarkar Gaurav
Keyword(s):  
Case Report ◽  
Male Infertility ◽  
Sperm Motility ◽  
Clinical Symptoms ◽  
Sperm Count ◽  
Single Case ◽  
Semen Analysis ◽  
Male Factor ◽  
Sperm Analysis ◽  
Patient Reported

Background: Male Infertility is one of the burning issues now a day’s nevertheless disregarded reproductive health problems in India. Incidences of this issue expands day by day because of the disturbing lifestyle pattern. Almost 30-40-% of infertility cases found to be related to male factor. Asthenozoospermia is the most common identifiable anomaly related to male infertility found in semen analysis having reduced motility of sperm. Aim and Objectives: To assess the efficacy of Ayurvedic management (Shodhana and Shamana Chikitsa) in the management of Ksheena Shukra Vikara w.s.r. to Asthenozoospermia. Methods: It is a single case study. A 33-year-old male patient who was already diagnosed with Asthenozoospermia for three years approached to Pancharkarma OPD. Sperm motility was only 12%. The patient was treated with Shodhana Chikitsa (Vamana and Virechana with Mahatiktaka Ghritapana and Dashmooladi Niruha Vasti and Uttarvasti with Vidaryadi Ghrita followed by Shamana Chikitsa (Tab Neo Charak Pharmacy, Tab Addyzoa, Chandraprabha Vati, Paripathadi Kashaya, Ashwagandhadi Yamaka, Avipattikar Churna) approximately 3 months. After 3 months, patient-reported improvement. Results: Assessment of the patient with clinical symptoms and sperm analysis report was done following 3 months. Sperm motility increased up to 40% with increment in sperm count.  Conclusion: This case report provides us a guideline that infertility associated with Asthenozoospermia can be treated successfully by adopting basic Ayurveda Siddhanta's.

scholarly journals Skin Inflammation and Testicular Function: Dermatitis Causes Male Infertility via Skin-Derived Cytokines

Biomedicines ◽  
2020 ◽  
Vol 8 (9) ◽  
pp. 293
Author(s):  
Ai Umaoka ◽  
Hiroki Takeuchi ◽  
Kento Mizutani ◽  
Naohiro Seo ◽  
Yoshiaki Matsushima ◽  
...  
Keyword(s):  
Mouse Model ◽  
Male Infertility ◽  
Inflammatory Cytokines ◽  
Sperm Count ◽  
Skin Inflammation ◽  
Testicular Function ◽  
Testis Size ◽  
Sperm Viability ◽  
Wild Type ◽  
The Relationship

The medical comorbidities including skin diseases are associated with male infertility. The most common cause of male infertility is the inability of testes to produce sperm; however, the influence of persistent dermatitis on testicular function has not been elucidated so far. We investigated the relationship between skin inflammation and impaired sperm production using a spontaneous dermatitis mouse model. We examined the breeding records of dermatitis mice and their wild-type littermates. Sperm count, motility, and viability were analyzed by direct microscopic observation and flow cytometry. In addition, testis and epididymis were histologically examined. Finally, sperm viability was evaluated in another dermatitis mouse model and in wild-type mice in which inflammatory cytokines were intraperitoneally administered. Compared to wild-type littermate mice, the number of children born was lower in mice with dermatitis. The body weight and testis size were decreased age-dependently. In the skin disease group, the sperm count and movement ratio were clearly decreased, and reduced sperm viability was observed. Histological examination revealed the detachment of Sertoli cells and reduced spermatogenesis. The fibrosis of epididymal stroma was severe, and it might affect defective sperm maturation in the epididymis. In addition, this phenomena was reproduced by a hapten applied dermatitis mouse model and the intraperitoneal administration of inflammatory cytokines. Once the skin is inflamed, inflammatory cytokines are produced and released, which may affect testicular and sperm function. Additional studies are needed to determine the relationship between male infertility and severe dermatitis in human.

scholarly journals PREVALANCE OF MALE INFERTILITY IN INDIA: STUDIES ON THE EFFECTS OF GONADOTROPIN RELEASING HORMONES

2017 ◽  
Vol 10 (8) ◽  
pp. 208
Author(s):  
Radhakrishnan Deventhiran ◽  
Kumaresan Ramanathan ◽  
Nagamurugan Nandakumar
Keyword(s):  
Male Infertility ◽  
Sperm Motility ◽  
Sperm Count ◽  
Follicle Stimulating Hormone ◽  
Sperm Concentration ◽  
Young Male ◽  
Lifestyle Changes ◽  
Infertile Men ◽  
Infertile Group ◽  
Stimulating Hormone

Objective: Nowadays, there is an increased incidence of infertility in Indian males due to lifestyle changes. Hence, the objective of this study isevaluating the gonadotropin releasing hormones (GnRH) level in infertile young male in Indian population.Materials and Methods: In total, 56 patients having abnormal semen count and five control patients have been included in the study. All patientswere underwent sperm count and estimation of hormones includes GnRH such as follicle stimulating hormone (FSH), tri-iodothyronine, thyroxin,prolactin, and testosterone.Results: The sperm concentration of infertile men was significantly lower than control. Sperm motility behaviors rapid, sluggish, and non-motilecharacters were significantly lower than control. Among GnRH, FSH has been significantly higher in infertile group than control group.Conclusion: FSH may be considered as a marker for male infertility.

scholarly journals Protein phosphatase 1 complexes modulate sperm motility and present novel targets for male infertility

2011 ◽  
Vol 17 (8) ◽  
pp. 466-477 ◽  
Author(s):  
M. Fardilha ◽  
S. L. C. Esteves ◽  
L. Korrodi-Gregorio ◽  
S. Pelech ◽  
O. A. B. da Cruz e Silva ◽  
...  
Keyword(s):  
Male Infertility ◽  
Sperm Motility ◽  
Protein Phosphatase ◽  
Protein Phosphatase 1 ◽  
Novel Targets

The Protective Effect of Metformin against Oxandrolone-Induced Infertility in Male Rats

2020 ◽  
Vol 26 ◽  
Author(s):  
Abdulqader Fadhil Abed ◽  
Yazun Bashir Jarrar ◽  
Hamzeh J Al-Ameer ◽  
Wajdy Al-Awaida ◽  
Su-Jun Lee
Keyword(s):  
Gene Expression ◽  
Male Infertility ◽  
Protective Effect ◽  
Histological Examination ◽  
Sperm Count ◽  
Serum Testosterone ◽  
Male Rats ◽  
P Value ◽  
Protective Effects ◽  
Rt Pcr

Background: Oxandrolone is a synthetic testosterone analogue that is widely used among bodybuilders and athletes. However, oxandrolone causes male infertility. Recently, it was found that metformin reduces the risk of infertility associated with diabetes mellitus. Aim: This study aimed to investigate the protective effects of metformin against oxandrolone-induced infertility in male rats. Methods: Rats continuously received one of four treatments (n=7) over 14 days: control DMSO administration, oxandrolone administration, metformin administration, or co-administration of oxandrolone and metformin. Doses were equivalent to those used for human treatment. Subsequently, testicular and blood samples were collected for morphological, biochemical, and histological examination. In addition, gene expression of the testosterone synthesizing enzyme CYP11A1 was analyzed in the testes using RT-PCR. Results: Oxandrolone administration induced male infertility by significantly reducing relative weights of testes by 48%, sperm count by 82%, and serum testosterone levels by 96% (ANOVA, P value < 0.05). In addition, histological examination determined that oxandrolone caused spermatogenic arrest which was associated with 2-fold downregulation of testicular CYP11A1 gene expression. However, co-administration of metformin with oxandrolone significantly ameliorated toxicological alterations induced by oxandrolone exposure (ANOVA, P value < 0.05). Conclusion: Metformin administration protected against oxandrolone-induced infertility in male rats. Further clinical studies are needed to confirm the protective effect of metformin against oxandrolone-induced infertility among athletes.

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