scholarly journals 1681Randomized Clinical Trial of the Recovery of Probiotics Lactobacillus rhamnosus GG (LGG) and Bifidobacterium animalis subspecies lactis (BB-12) from the Gastrointestinal (GI) Tract of Healthy Volunteers

2014 ◽  
Vol 1 (suppl_1) ◽  
pp. S449-S449
Author(s):  
Debra D. Poutsiaka ◽  
Lisa E. Davidson ◽  
Lori Lathrop Stern ◽  
Cheleste M. Thorpe ◽  
Anne V. Kane ◽  
...  
Keyword(s):  
Clinical Trial ◽  
Healthy Volunteers ◽  
Lactobacillus Rhamnosus ◽  
Gi Tract ◽  
Lactobacillus Rhamnosus Gg ◽  
Bifidobacterium Animalis

The Impact of Storage Conditions on the Stability of Lactobacillus rhamnosus GG and Bifidobacterium animalis subsp. lactis Bb12 in Human Milk

2017 ◽  
Vol 12 (9) ◽  
pp. 566-569 ◽  
Author(s):  
Anastasia Mantziari ◽  
Juhani Aakko ◽  
Himanshu Kumar ◽  
Satu Tölkkö ◽  
Elloise du Toit ◽  
...  
Keyword(s):  
Human Milk ◽  
Lactobacillus Rhamnosus ◽  
Storage Conditions ◽  
Lactobacillus Rhamnosus Gg ◽  
Bifidobacterium Animalis ◽  
The Stability ◽  
The Impact

scholarly journals In Vitro Effects of Live and Heat-Inactivated Bifidobacterium animalis Subsp. Lactis, BB-12 and Lactobacillus rhamnosus GG on Caco-2 Cells

Nutrients ◽  
2020 ◽  
Vol 12 (6) ◽  
pp. 1719
Author(s):  
Vivian M. Castro-Herrera ◽  
Christine Rasmussen ◽  
Anja Wellejus ◽  
Elizabeth A. Miles ◽  
Philip C. Calder
Keyword(s):  
Inflammatory Mediators ◽  
Lactobacillus Rhamnosus ◽  
Lactobacillus Rhamnosus Gg ◽  
Bifidobacterium Animalis ◽  
Host Interaction ◽  
Heat Treated ◽  
Pre Treatment ◽  
In Vitro Effects ◽  
Endothelial Growth Factor

Probiotic–host interaction can be cell-to-cell or through metabolite production. Dead (inactive) organisms could interact with the host, leading to local effects and possible health benefits. This research examined the effects of live and heat-inactivated Bifidobacterium animalis subsp. lactis, BB-12 (BB-12) and Lactobacillus rhamnosus GG (LGG) on cultured Caco-2 cells focusing on epithelial integrity and production of inflammatory mediators. Live organisms increased transepithelial electrical resistance (TEER), a barrier-integrity marker, with LGG having a greater effect than BB-12. When mildly heat-treated, both organisms had a more modest effect on TEER than when alive. When they were heat-inactivated, both organisms had only a limited effect on TEER. Neither live nor heat-inactivated organisms affected production of six inflammatory mediators produced by Caco-2 cells compared to control conditions. Pre-treatment with heat-inactivated LGG or BB-12 did not alter the decline in TEER caused by exposure to an inflammatory cocktail of cytokines. However, pre-treatment of Caco-2 cells with heat-inactivated organisms alone or their combination decreased the production of interleukin (IL)-6, IL-18, and vascular endothelial growth factor. To conclude, while the live organisms improve the epithelial barrier using this model, neither live nor heat-inactivated organisms directly elicit an inflammatory response by the epithelium. Pre-treatment with heat-inactivated BB-12 or LGG can reduce some components of the response induced by an inflammatory stimulus.

scholarly journals Influence of Probiotics on the Salivary Microflora Oral Streptococci and Their Integration into Oral Biofilm

Antibiotics ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 803
Author(s):  
Nicole B. Arweiler ◽  
Thorsten M. Auschill ◽  
Christian Heumann ◽  
Elmar Hellwig ◽  
Ali Al-Ahmad
Keyword(s):  
Clinical Trial ◽  
Enterococcus Faecalis ◽  
Bacterial Load ◽  
Lactobacillus Rhamnosus ◽  
Probiotic Bacteria ◽  
Oral Streptococci ◽  
Oral Biofilm ◽  
Lactobacillus Rhamnosus Gg ◽  
Dental Biofilm ◽  
Oral Biofilms

Probiotics’ ability to integrate into dental biofilms is not yet clarified. The aim of this trial was to detect probiotic bacteria from probiotic products in dental biofilm and saliva during and after intake. In this parallel, randomized clinical trial, 39 subjects wore customized appliances to build up intra-oral biofilms (72-h periods). The trial was divided into screening (S) to determine baseline biofilm flora, intervention (I), and wash out (WO). During I (28 days), subjects consumed a product containing (a) Enterococcus faecalis (b) Lactobacilluscasei, or (c) Lactobacillus rhamnosus GG. Probiotic bacteria and Streptococci spp. were detected in the biofilms and saliva of the 35 subjects that were included in the analysis. During I and WO, the ratio of probiotics in the biofilm was very low compared to total bacterial load, while saliva had slightly but not significantly higher values. No significant changes of probiotic bacteria (p > 0.05) were found at any visit during I or WO. The proportion of streptococci was significantly reduced (p < 0.05) during I and even lower in WO, compared to S. Probiotic bacteria could neither integrate nor persist in dental biofilm and saliva but did influence the growth of streptococci in biofilm and saliva.

scholarly journals Influence of Three Probiotics Strains, Lactobacillus rhamnosus GG, Bifidobacterium animalis subsp. Lactis BB-12 and Saccharomyces boulardii CNCM I-745 on the Biochemical and Haematological Profiles and Body Weight of Healthy Rabbits

Biology ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 1194
Author(s):  
Louiza Kadja ◽  
Amira Leila Dib ◽  
Nedjoua Lakhdara ◽  
Assia Bouaziz ◽  
Elena Espigares ◽  
...  
Keyword(s):  
Body Weight ◽  
Muscle Growth ◽  
Lactobacillus Rhamnosus ◽  
Blood Parameters ◽  
Saccharomyces Boulardii ◽  
The Body ◽  
Lactobacillus Rhamnosus Gg ◽  
Positive Effects ◽  
Bifidobacterium Animalis ◽  
Underlying Mechanisms

This study aimed to investigate the effects of three strains of probiotics, these being Lactobacillus rhamnosus GG, Bifidobacterium animalis subsp. Lactis BB-12 and Saccharomyces boulardii CNCM I-745, on the body weight, animal performances and blood parameters of rabbits (male and female) of the ITELV2006 strain. The supplementation of the feed of the rabbits with the three probiotic strains allowed observing positive effects on most of the biochemical and haematological parameters investigated during a period of 60 days (30 days of supplementation and 30 days without treatment). Further, there was a significant improvement in the body weight of the rabbits at the end of the experiment. The effect of the three probiotics investigated in this trial was found to be related to the sex of the rabbits and to the intake period (duration). Ultimately, these findings raise the possibility of using probiotics to investigate in an in-depth and specific manner based on fixed factors such as the strain, the gender and age of the animals, the main underlying mechanisms and effects, which would allow achieving optimal and adapted health benefits and sustainable production. In the context of animal production, it is worth investigating in a targeted study the effect of the three strains on muscle growth and development and finding evidence of the possible consequences on meat quality traits of the rabbits supplemented with probiotics.

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