A Subset of Arabidopsis RAV Transcription Factors Modulates Drought and Salt Stress Responses Independent of ABA

Abstract Background Salt stress threatens crop yields all over the world. Many NAC transcription factors have been reported to be involved in different abiotic stress responses, but it remains unclear how loss of these transcription factors alters the transcriptomes of plants. Previous reports have demonstrated that overexpression of OsNAC45 enhances salt and drought tolerance in rice, and that OsNAC45 may regulate the expression of two specific genes, OsPM1 and OsLEA3–1. Results Here, we found that ABA repressed, and NaCl promoted, the expression of OsNAC45 in roots. Immunostaining showed that OsNAC45 was localized in all root cells and was mainly expressed in the stele. Loss of OsNAC45 decreased the sensitivity of rice plants to ABA and over-expressing this gene had the opposite effect, which demonstrated that OsNAC45 played an important role during ABA signal responses. Knockout of OsNAC45 also resulted in more ROS accumulation in roots and increased sensitivity of rice to salt stress. Transcriptome sequencing assay found that thousands of genes were differently expressed in OsNAC45-knockout plants. Most of the down-regulated genes participated in plant stress responses. Quantitative real time RT-PCR suggested that seven genes may be regulated by OsNAC45 including OsCYP89G1, OsDREB1F, OsEREBP2, OsERF104, OsPM1, OsSAMDC2, and OsSIK1. Conclusions These results indicate that OsNAC45 plays vital roles in ABA signal responses and salt tolerance in rice. Further characterization of this gene may help us understand ABA signal pathway and breed rice plants that are more tolerant to salt stress.

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GhWRKY46 from Upland Cotton positively Regulates the Drought and Salt stress Responses in Plant

Environmental and Experimental Botany ◽

10.1016/j.envexpbot.2021.104438 ◽

2021 ◽

pp. 104438

Author(s):

Yu Li ◽

Hao Chen ◽

Shengting Li ◽

Cuiling Yang ◽

Qunying Ding ◽

...

Keyword(s):

Salt Stress ◽

Stress Responses ◽

Upland Cotton ◽

Drought And Salt Stress

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Expression Analyses of Soybean VOZ Transcription Factors and the Role of GmVOZ1G in Drought and Salt Stress Tolerance

International Journal of Molecular Sciences ◽

10.3390/ijms21062177 ◽

2020 ◽

Vol 21 (6) ◽

pp. 2177 ◽

Cited By ~ 1

Author(s):

Bo Li ◽

Jia-Cheng Zheng ◽

Ting-Ting Wang ◽

Dong-Hong Min ◽

Wen-Liang Wei ◽

...

Keyword(s):

Transcription Factor ◽

Transcription Factors ◽

Salt Stress ◽

Abiotic Stress ◽

Stress Tolerance ◽

Zinc Finger ◽

Hairy Roots ◽

Yeast Cells ◽

Salt Stress Tolerance ◽

Drought And Salt Stress

Vascular plant one-zinc-finger (VOZ) transcription factor, a plant specific one-zinc-finger-type transcriptional activator, is involved in regulating numerous biological processes such as floral induction and development, defense against pathogens, and response to multiple types of abiotic stress. Six VOZ transcription factor-encoding genes (GmVOZs) have been reported to exist in the soybean (Glycine max) genome. In spite of this, little information is currently available regarding GmVOZs. In this study, GmVOZs were cloned and characterized. GmVOZ genes encode proteins possessing transcriptional activation activity in yeast cells. GmVOZ1E, GmVOZ2B, and GmVOZ2D gene products were widely dispersed in the cytosol, while GmVOZ1G was primarily located in the nucleus. GmVOZs displayed a differential expression profile under dehydration, salt, and salicylic acid (SA) stress conditions. Among them, GmVOZ1G showed a significantly induced expression in response to all stress treatments. Overexpression of GmVOZ1G in soybean hairy roots resulted in a greater tolerance to drought and salt stress. In contrast, RNA interference (RNAi) soybean hairy roots suppressing GmVOZ1G were more sensitive to both of these stresses. Under drought treatment, soybean composite plants with an overexpression of hairy roots had higher relative water content (RWC). In response to drought and salt stress, lower malondialdehyde (MDA) accumulation and higher peroxidase (POD) and superoxide dismutase (SOD) activities were observed in soybean composite seedlings with an overexpression of hairy roots. The opposite results for each physiological parameter were obtained in RNAi lines. In conclusion, GmVOZ1G positively regulates drought and salt stress tolerance in soybean hairy roots. Our results will be valuable for the functional characterization of soybean VOZ transcription factors under abiotic stress.

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AtERF60 negatively regulates ABR1 to modulate salt, drought, and basal resistance in Arabidopsis

10.1101/2021.12.29.474403 ◽

2021 ◽

Author(s):

Gajendra Singh Jeena ◽

Ujjal Jyoti Phukan ◽

Neeti Singh ◽

Ashutosh Joshi ◽

Alok Pandey ◽

...

Keyword(s):

Abscisic Acid ◽

Salt Stress ◽

Stress Responses ◽

Biotic Stress ◽

Bacterial Pathogen ◽

Pcr Analysis ◽

Cis Elements ◽

Abiotic And Biotic Stress ◽

Basal Resistance ◽

Drought And Salt Stress

ABSCISIC ACID REPRESSOR-1 (ABR1), an APETALA2 (AP2) domain containing transcription factor (TF) contribute important function against variety of external cues. Here, we report an AP2/ERF TF, AtERF60 that serves as an important regulator of ABR1 gene. AtERF60 is induced in response to drought, salt, abscisic acid (ABA), salicylic acid (SA), and bacterial pathogen PstDC3000 infection. AtERF60 interacts with DEHYDRATION RESPONSE ELEMENTS (DRE1/2) and GCC box indicating its ability to regulate multiple responses. Overexpression of AtERF60 results in the drought and salt stress tolerant phenotype in both seedling and mature Arabidopsis plants in comparison with the wild type (WT-Col). However, mutation in AtERF60 showed hyperactive response against drought and salt stress in comparison with its overexpression and WT. Microarray and qRT-PCR analysis of overexpression and mutant lines indicated that AtERF60 regulates both abiotic and biotic stress inducible genes. One of the differentially expressing transcripts was ABR1 and we found that AtERF60 interacts with the DRE cis-elements present in the ABR1 promoter. The mutation in AtERF60 showed ABA hypersensitive response, increased ABA content, and reduced susceptibility to PstDC3000. Altogether, we conclude that AtERF60 represses ABR1 transcript by binding with the DRE cis-elements and modulates both abiotic and biotic stress responses in Arabidopsis.

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Understanding the Integrated Pathways and Mechanisms of Transporters, Protein Kinases, and Transcription Factors in Plants under Salt Stress

International Journal of Genomics ◽

10.1155/2021/5578727 ◽

2021 ◽

Vol 2021 ◽

pp. 1-16

Author(s):

Wasifa Hafiz Shah ◽

Aadil Rasool ◽

Seerat Saleem ◽

Naveed Ul Mushtaq ◽

Inayatullah Tahir ◽

...

Keyword(s):

Transcription Factors ◽

Salt Stress ◽

Protein Kinases ◽

Stress Responses ◽

Crop Improvement ◽

Ion Homeostasis ◽

Underlying Mechanism ◽

Regulatory Proteins ◽

Stress Signalling

Abiotic stress is the major threat confronted by modern-day agriculture. Salinity is one of the major abiotic stresses that influence geographical distribution, survival, and productivity of various crops across the globe. Plants perceive salt stress cues and communicate specific signals, which lead to the initiation of defence response against it. Stress signalling involves the transporters, which are critical for water transport and ion homeostasis. Various cytoplasmic components like calcium and kinases are critical for any type of signalling within the cell which elicits molecular responses. Stress signalling instils regulatory proteins and transcription factors (TFs), which induce stress-responsive genes. In this review, we discuss the role of ion transporters, protein kinases, and TFs in plants to overcome the salt stress. Understanding stress responses by components collectively will enhance our ability in understanding the underlying mechanism, which could be utilized for crop improvement strategies for achieving food security.

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A Wheat R2R3-type MYB Transcription Factor TaODORANT1 Positively Regulates Drought and Salt Stress Responses in Transgenic Tobacco Plants

Frontiers in Plant Science ◽

10.3389/fpls.2017.01374 ◽

2017 ◽

Vol 8 ◽

Cited By ~ 46

Author(s):

Qiuhui Wei ◽

Qingchen Luo ◽

Ruibin Wang ◽

Fan Zhang ◽

Yuan He ◽

...

Keyword(s):

Transcription Factor ◽

Salt Stress ◽

Transgenic Tobacco ◽

Stress Responses ◽

Myb Transcription Factor ◽

Transgenic Tobacco Plants ◽

Tobacco Plants ◽

Drought And Salt Stress

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Genome-wide analysis of AP2/ERF Transcription Factors in sugarcane Saccharum spontaneum L. Reveals Functional Divergence During Drought, Salt Stress and Plant Hormones Treatment

10.21203/rs.3.rs-19836/v1 ◽

2020 ◽

Cited By ~ 1

Author(s):

Piting Li ◽

Zhe Chai ◽

Pingping Lin ◽

Chaohua Huang ◽

Guoqiang Huang ◽

...

Keyword(s):

Transcription Factors ◽

Salt Stress ◽

Drought Stress ◽

Stress Responses ◽

Environmental Changes ◽

Expression Patterns ◽

Regulatory Elements ◽

Structure Evolution ◽

Saccharum Spontaneum ◽

Erf Transcription Factors

Abstract Background: APETALA2/ETHYLENE RESPONSIVE FACTOR (AP2/ERF) transcription factors play important roles in plant growth, development, metabolism, as well as in biotic and abiotic stress responses. However, there are few studies concerning AP2/ERF genes in sugarcane, which is the most critical sugar and energy crop worldwide. Results: A total of 218 AP2/ERF genes were identified in the Saccharum spontaneum genome. Phylogenetic analysis showed that these genes could be divided into four groups, including 43 AP2s, 160 ERFs, and Dehydration-responsive element-binding (DREB) factors, 11 ABI3/VPs (RAV) and 4 Soloist genes. These genes were unevenly distributed on 32 chromosomes. Analysis of the structural of SsAP2/ERF genes showed that 91 SsAP2/ERFs lacked introns. Sugarcane and sorghum have a collinear relationship between 168 SsAP2/ERF genes and sorghum AP2/ERF genes that reflects their similarity. Multiple cis-regulatory elements (CREs) are present in the SsAP2/ERF promoter, and many are related to abiotic stresses, suggesting that SsAP2/ERF activity could contribute to the adaptation of sugarcane crops to environmental changes. The tissue-specific analysis showed spatiotemporal expression of SsAP2/ERF in the stems and leaves of sugarcane at different stages of development. In 10 sugarcane samples, 39 SsAP2/ERFs were not expressed at all, whereas 58 SsAP2/ERFs were expressed in all samples. Quantitative PCR experiments showed that SsERF52 expression was up-regulated under salt stress, but suppressed under drought stress. SsSoloist4 had the most considerable upregulation in response to treatment with the exogenous hormones ABA and GA. Within 3 hours of ABA or PEG6000 treatment, SsSoloist4 expression was up-regulated, indicating that this gene could play a role in ABA and GA-associated drought stress response mechanisms. Analysis of AP2/ERF gene expression patterns under different treatments indicated that SsAP2/ERF genes play an important role in drought and salt stress responses of S. spontaneum. Conclusions: In this study, a total of 218 members of the AP2 / ERF superfamily were identified in sugarcane, and their genetic structure, evolution characteristics, and expression patterns were studied and analyzed. The results of this study provide a foundation for future analyses to elucidate the importance of AP2/ERF transcription factors in the function and molecular breeding of sugarcane.

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Arabidopsis Annexins AnnAt1 and AnnAt4 Interact with Each Other and Regulate Drought and Salt Stress Responses

Plant and Cell Physiology ◽

10.1093/pcp/pcq111 ◽

2010 ◽

Vol 51 (9) ◽

pp. 1499-1514 ◽

Cited By ~ 74

Author(s):

Sun Mi Huh ◽

Eun Kyeung Noh ◽

Hye Gi Kim ◽

Byeong Wook Jeon ◽

Kisuk Bae ◽

...

Keyword(s):

Salt Stress ◽

Stress Responses ◽

Drought And Salt Stress

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Identification and characterization of two faba bean (Vicia faba L.) WRKY transcription factors and their expression analysis during salt and drought stress

The Journal of Agricultural Science ◽

10.1017/s0021859616000885 ◽

2016 ◽

Vol 155 (5) ◽

pp. 791-803 ◽

Cited By ~ 3

Author(s):

G. ABID ◽

Y. MUHOVSKI ◽

D. MINGEOT ◽

M. N. SAIDI ◽

M. AOUIDA ◽

...

Keyword(s):

Transcription Factors ◽

Salt Stress ◽

Faba Bean ◽

Expression Patterns ◽

Pcr Analysis ◽

Potential Candidate ◽

Degenerate Primers ◽

Salt Stress Response ◽

Group I ◽

Drought And Salt Stress

SUMMARYDrought and salinity are two major environmental factors limiting faba bean growth, leading to considerable reduction in their productivity. The WRKY gene family act as major transcription factors that might play an important role in abiotic stress tolerance. In the present study, two partial sequences sharing significant homology with known WRKY genes were isolated from faba bean by polymerase chain reaction (PCR) amplification using degenerate primers targeting the well-conserved WRKY domain. The isolated WRKY gene fragments were designated as VfWRKY1 and VfWRKY2 showing 62% similarity between them. Sequence and phylogenetic analyses revealed that VfWRKY1 and VfWRKY2 belong to WRKY group I and could be grouped with their orthologues from other plant species. The gene expression profile of VfWRKY1 and VfWRKY2 in faba bean showed that they are significantly accumulated in various plant organs. Further, quantitative real-time PCR analysis showed that both transcripts were responsive to drought and salt stress, and also they are genotype dependent, meaning that different faba bean cultivars respond in a different way to drought and salt challenge. The expression patterns obtained suggest the important roles of VfWRKY1 and VfWRKY2 in drought and salt stress response and tolerance. This knowledge might be helpful in the identification of drought-tolerant cultivars and provide potential candidate markers for faba bean breeding in order to develop osmotic-stress-tolerant cultivars.

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