Arabidopsis Annexins AnnAt1 and AnnAt4 Interact with Each Other and Regulate Drought and Salt Stress Responses

ABSCISIC ACID REPRESSOR-1 (ABR1), an APETALA2 (AP2) domain containing transcription factor (TF) contribute important function against variety of external cues. Here, we report an AP2/ERF TF, AtERF60 that serves as an important regulator of ABR1 gene. AtERF60 is induced in response to drought, salt, abscisic acid (ABA), salicylic acid (SA), and bacterial pathogen PstDC3000 infection. AtERF60 interacts with DEHYDRATION RESPONSE ELEMENTS (DRE1/2) and GCC box indicating its ability to regulate multiple responses. Overexpression of AtERF60 results in the drought and salt stress tolerant phenotype in both seedling and mature Arabidopsis plants in comparison with the wild type (WT-Col). However, mutation in AtERF60 showed hyperactive response against drought and salt stress in comparison with its overexpression and WT. Microarray and qRT-PCR analysis of overexpression and mutant lines indicated that AtERF60 regulates both abiotic and biotic stress inducible genes. One of the differentially expressing transcripts was ABR1 and we found that AtERF60 interacts with the DRE cis-elements present in the ABR1 promoter. The mutation in AtERF60 showed ABA hypersensitive response, increased ABA content, and reduced susceptibility to PstDC3000. Altogether, we conclude that AtERF60 represses ABR1 transcript by binding with the DRE cis-elements and modulates both abiotic and biotic stress responses in Arabidopsis.

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A Wheat R2R3-type MYB Transcription Factor TaODORANT1 Positively Regulates Drought and Salt Stress Responses in Transgenic Tobacco Plants

Frontiers in Plant Science ◽

10.3389/fpls.2017.01374 ◽

2017 ◽

Vol 8 ◽

Cited By ~ 46

Author(s):

Qiuhui Wei ◽

Qingchen Luo ◽

Ruibin Wang ◽

Fan Zhang ◽

Yuan He ◽

...

Keyword(s):

Transcription Factor ◽

Salt Stress ◽

Transgenic Tobacco ◽

Stress Responses ◽

Myb Transcription Factor ◽

Transgenic Tobacco Plants ◽

Tobacco Plants ◽

Drought And Salt Stress

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Characterization of an inositol 1,3,4-trisphosphate 5/6-kinase gene that is essential for drought and salt stress responses in rice

Plant Molecular Biology ◽

10.1007/s11103-011-9830-9 ◽

2011 ◽

Vol 77 (6) ◽

pp. 547-563 ◽

Cited By ~ 35

Author(s):

Hao Du ◽

Linhong Liu ◽

Lei You ◽

Mei Yang ◽

Yubing He ◽

...

Keyword(s):

Salt Stress ◽

Stress Responses ◽

Kinase Gene ◽

Drought And Salt Stress

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Overexpression of GhMPK3 from Cotton Enhances Cold, Drought, and Salt Stress in Arabidopsis

Agronomy ◽

10.3390/agronomy11061049 ◽

2021 ◽

Vol 11 (6) ◽

pp. 1049

Author(s):

Salisu Bello Sadau ◽

Adeel Ahmad ◽

Sani Muhammad Tajo ◽

Sani Ibrahim ◽

Bello Babatunde Kazeem ◽

...

Keyword(s):

Salt Stress ◽

Abiotic Stress ◽

Stress Responses ◽

Abiotic Stresses ◽

Leaf Water ◽

Mitogen Activated Protein Kinase ◽

Leaf Water Content ◽

Ion Leakage ◽

Cotton Production ◽

Drought And Salt Stress

Cotton production is hampered by a variety of abiotic stresses that wreak havoc on the growth and development of plants, resulting in significant financial losses. According to reports, cotton production areas have declined around the world as a result of the ongoing stress. Therefore, plant breeding programs are concentrating on abiotic stress-tolerant cotton varieties. Mitogen-activated protein kinase (MAPK) cascades are involved in plant growth, stress responses, and the hormonal signaling pathway. In this research, three abiotic stresses (cold, drought, and salt) were analyzed on GhMPK3 transformed Arabidopsis plants. The transgenic plant’s gene expression and morphologic analysis were studied under cold, drought, and salt stress. Physiological parameters such as relative leaf water content, excised leaf water loss, chlorophyll content, and ion leakage showed that overexpressed plants possess more stable content under stress conditions compared with the WT plants. Furthermore, GhMPK3 overexpressed plants had greater antioxidant activities and weaker oxidant activities. Silencing GhMPK3 in cotton inhibited its tolerance to drought stress. Our research findings strongly suggest that GhMPK3 can be regarded as an essential gene for abiotic stress tolerance in cotton plants.

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ZmACY-1 Antagonistically Regulates Growth and Stress Responses in Nicotiana benthamiana

Frontiers in Plant Science ◽

10.3389/fpls.2021.593001 ◽

2021 ◽

Vol 12 ◽

Author(s):

Dongbin Chen ◽

Junhua Li ◽

Fuchao Jiao ◽

Qianqian Wang ◽

Jun Li ◽

...

Keyword(s):

Salt Stress ◽

Abiotic Stress ◽

Plant Growth ◽

Stress Responses ◽

Nicotiana Benthamiana ◽

Expression Patterns ◽

Balance Of Plant ◽

Drought And Salt Stress ◽

Oxidative Stress Responses ◽

And Oxidative Stress

Aminoacylase-1 is a zinc-binding enzyme that is important in urea cycling, ammonia scavenging, and oxidative stress responses in animals. Aminoacylase-1 (ACY-1) has been reported to play a role in resistance to pathogen infection in the model plant Nicotiana benthamiana. However, little is known about its function in plant growth and abiotic stress responses. In this study, we cloned and analyzed expression patterns of ZmACY-1 in Zea mays under different conditions. We also functionally characterized ZmACY-1 in N. benthamiana. We found that ZmACY-1 is expressed specifically in mature shoots compared with other tissues. ZmACY-1 is repressed by salt, drought, jasmonic acid, and salicylic acid, but is induced by abscisic acid and ethylene, indicating a potential role in stress responses and plant growth. The overexpression of ZmACY-1 in N. benthamiana promoted growth rate by promoting growth-related genes, such as NbEXPA1 and NbEIN2. At the same time, the overexpression of ZmACY-1 in N. benthamiana reduced tolerance to drought and salt stress. With drought and salt stress, the activity of protective enzymes, such as peroxidase (POD), superoxide dismutase (SOD), and catalase (CAT) from micrococcus lysodeikticus was lower; while the content of malondialdehyde (MDA) and relative electrolytic leakage was higher in ZmACY-1 overexpression lines than that in wild-type lines. The results indicate that ZmACY-1 plays an important role in the balance of plant growth and defense and can be used to assist plant breeding under abiotic stress conditions.

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Faculty Opinions recommendation of Salt stress responses in Arabidopsis utilize a signal transduction pathway related to endoplasmic reticulum stress signaling.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1096008.551993 ◽

2007 ◽

Author(s):

Ramón Serrano

Keyword(s):

Signal Transduction ◽

Endoplasmic Reticulum ◽

Salt Stress ◽

Endoplasmic Reticulum Stress ◽

Stress Responses ◽

Signal Transduction Pathway ◽

Stress Signaling ◽

Transduction Pathway

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OsNAC45 is Involved in ABA Response and Salt Tolerance in Rice

Rice ◽

10.1186/s12284-020-00440-1 ◽

2020 ◽

Vol 13 (1) ◽

Author(s):

Xiang Zhang ◽

Yan Long ◽

Jingjing Huang ◽

Jixing Xia

Keyword(s):

Transcription Factors ◽

Salt Stress ◽

Salt Tolerance ◽

Stress Responses ◽

Crop Yields ◽

Plant Stress ◽

Root Cells ◽

Nac Transcription Factors ◽

Rice Plants ◽

Plant Stress Responses

Abstract Background Salt stress threatens crop yields all over the world. Many NAC transcription factors have been reported to be involved in different abiotic stress responses, but it remains unclear how loss of these transcription factors alters the transcriptomes of plants. Previous reports have demonstrated that overexpression of OsNAC45 enhances salt and drought tolerance in rice, and that OsNAC45 may regulate the expression of two specific genes, OsPM1 and OsLEA3–1. Results Here, we found that ABA repressed, and NaCl promoted, the expression of OsNAC45 in roots. Immunostaining showed that OsNAC45 was localized in all root cells and was mainly expressed in the stele. Loss of OsNAC45 decreased the sensitivity of rice plants to ABA and over-expressing this gene had the opposite effect, which demonstrated that OsNAC45 played an important role during ABA signal responses. Knockout of OsNAC45 also resulted in more ROS accumulation in roots and increased sensitivity of rice to salt stress. Transcriptome sequencing assay found that thousands of genes were differently expressed in OsNAC45-knockout plants. Most of the down-regulated genes participated in plant stress responses. Quantitative real time RT-PCR suggested that seven genes may be regulated by OsNAC45 including OsCYP89G1, OsDREB1F, OsEREBP2, OsERF104, OsPM1, OsSAMDC2, and OsSIK1. Conclusions These results indicate that OsNAC45 plays vital roles in ABA signal responses and salt tolerance in rice. Further characterization of this gene may help us understand ABA signal pathway and breed rice plants that are more tolerant to salt stress.

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Exogenous Auxin-Mediated Salt Stress Alleviation in Faba Bean (Vicia faba L.)

Agronomy ◽

10.3390/agronomy11030547 ◽

2021 ◽

Vol 11 (3) ◽

pp. 547

Author(s):

Arafat Abdel Hamed Abdel Latef ◽

Md. Tahjib-Ul-Arif ◽

Mohammad Saidur Rhaman

Keyword(s):

Salt Stress ◽

Vicia Faba ◽

Faba Bean ◽

Stress Responses ◽

Foliar Application ◽

Soluble Sugar ◽

Principal Component ◽

Vicia Faba L ◽

Catalase Peroxidase ◽

Amino Acid Contents

Auxin not only controls the development processes, but also regulates the stress responses of plants. In this investigation, we explored the potential roles of exogenously applied indole-3-acetic acid (IAA) in conferring salt tolerance in the faba bean (Vicia faba L.). Our results showed that foliar application of IAA (200 ppm) to salt-exposed (60 mM and 150 mM NaCl) plants promoted growth, which was evidenced by enhanced root–stem traits. IAA application ensured better osmotic protection in salt-stressed plants which was supported by reduced proline and enhanced soluble sugar, soluble protein, and total free amino acid contents in the roots, stem, and seeds. IAA application also increased the number of nodules in salt-stressed plants, which may facilitate better nitrogen assimilation. Moreover, IAA mediated improvements in mineral homeostasis (K+, Ca2+, and Mg2+) and the translocation of Na+, while it also inhibited excessive accumulation of Na+ in the roots. Salt-induced oxidative damage resulted in increased accumulation of malondialdehyde, whereas IAA spraying relegated malondialdehyde by improving antioxidant enzymes, including superoxide dismutase, catalase, peroxidase, and ascorbate peroxidase. Together, these results together with a principal component analysis uncovered that foliar spraying of IAA alleviated the antagonistic effects of salt stress via enhancing osmolyte accumulation, ionic homeostasis, and antioxidant activity. Finally, exogenous IAA enhanced the yield of broad beans under high salinity conditions.

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