Vascularized Bone Regeneration Accelerated by 3D-Printed Nanosilicate-Functionalized Polycaprolactone Scaffold

Abstract Critical oral-maxillofacial bone defects, damaged by trauma and tumors, not only affect the physiological functions and mental health of patients but are also highly challenging to reconstruct. Personalized biomaterials customized by 3D printing technology have the potential to match oral-maxillofacial bone repair and regeneration requirements. Laponite nanosilicates have been added to biomaterials to achieve biofunctional modification owing to their excellent biocompatibility and bioactivity. Herein, porous nanosilicate-functionalized polycaprolactone (PCL/LAP) was fabricated by 3D printing technology, and its bioactivities in bone regeneration were investigated in vitro and in vivo. In vitro experiments demonstrated that PCL/LAP exhibited good cytocompatibility and enhanced the viability of BMSCs. PCL/LAP functioned to stimulate osteogenic differentiation of BMSCs at the mRNA and protein levels and elevated angiogenic gene expression and cytokine secretion. Moreover, BMSCs cultured on PCL/LAP promoted the angiogenesis potential of endothelial cells by angiogenic cytokine secretion. Then, PCL/LAP scaffolds were implanted into the calvarial defect model. Toxicological safety of PCL/LAP was confirmed, and significant enhancement of vascularized bone formation was observed. Taken together, 3D-printed PCL/LAP scaffolds with brilliant osteogenesis to enhance bone regeneration could be envisaged as an outstanding bone substitute for a promising change in oral-maxillofacial bone defect reconstruction.

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In Vitro and In Vivo Bioequivalence Study of 3D-Printed Instant-Dissolving Levetiracetam Tablets and Subsequent Personalized Dosing for Chinese Children Based on Physiological Pharmacokinetic Modeling

Pharmaceutics ◽

10.3390/pharmaceutics14010020 ◽

2021 ◽

Vol 14 (1) ◽

pp. 20

Author(s):

Xianfu Li ◽

En Liang ◽

Xiaoxuan Hong ◽

Xiaolu Han ◽

Conghui Li ◽

...

Keyword(s):

3D Printing ◽

Pbpk Model ◽

Dose Range ◽

Chinese Children ◽

Pbpk Modeling ◽

Printing Technology ◽

3D Printing Technology ◽

Pbpk Models ◽

3D Printed

Recently, the development of Binder Jet 3D printing technology has promoted the research and application of personalized formulations, which are especially useful for children’s medications. Additionally, physiological pharmacokinetic (PBPK) modeling can be used to guide drug development and drug dose selection. Multiple technologies can be used in combination to increase the safety and effectiveness of drug administration. In this study, we performed in vivo pharmacokinetic experiments in dogs with preprepared 3D-printed levetiracetam instant-dissolving tablets (LEV-IDTs). Bioequivalence analysis showed that the tablets were bioequivalent to commercially available preparations (Spritam®) for dogs. Additionally, we evaluated the bioequivalence of 3D-printed LEV-IDTs with Spritam® by a population-based simulation based on the established PBPK model of levetiracetam for Chinese adults. Finally, we established a PBPK model of oral levetiracetam in Chinese children by combining the physiological parameters of children, and we simulated the PK (pharmacokinetics) curves of Chinese children aged 4 and 6 years that were administered the drug to provide precise guidance on adjusting the dose according to the effective dose range of the drug. Briefly, utilizing both Binder jet 3D printing technology and PBPK models is a promising route for personalized drug delivery with various age groups.

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Three-Dimensional-Printed Poly-L-Lactic Acid Scaffolds with Different Pore Sizes Influence Periosteal Distraction Osteogenesis of a Rabbit Skull

BioMed Research International ◽

10.1155/2020/7381391 ◽

2020 ◽

Vol 2020 ◽

pp. 1-14

Author(s):

Danyang Zhao ◽

Wenbo Jiang ◽

Yu Wang ◽

Chuandong Wang ◽

Xiaoling Zhang ◽

...

Keyword(s):

Lactic Acid ◽

3D Printing ◽

Distraction Osteogenesis ◽

Animal Experiments ◽

Three Dimensional ◽

Printing Technology ◽

Pore Sizes ◽

3D Printing Technology

The repair of bone defects is a big challenge in reconstructive surgery. Periosteal distraction osteogenesis (PDO), as a promising technique used for bone regeneration, forms a space between the periosteum and bone cortex to regenerate the new bone merely by distracting the periosteum. In order to investigate the influence of distractor framework on the PDO, we utilized three-dimensional (3D) printing technology to fabricate three kinds of poly-L-lactic acid (PLLA) scaffolds with different pore sizes in this study. The in vitro experiments showed that the customized PLLA scaffolds had different-sized microchannels with low toxicity, good biocompatibility, and enough mechanical strength. Then, we built up an in vivo bioreactor under the skull periosteum of New Zealand white rabbits. The distractors with different pore sizes all could satisfy the demand of periosteal distraction in the animal experiments. After 8 weeks of consolidation period, the quality and quantity of the newly formed bone were improved with the increasing pore sizes of the distractors. Moreover, the newly formed bone also displayed an increasing degree of vascularization. In conclusion, 3D printing technology could promote the innovation of PDO devices and fabricate optimized scaffolds with appropriate pore sizes, shapes, and structures. It would help us regenerate more functional tissue-engineered bone and provide new ideas for further clinical application of the PDO technique.

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A millifluidic chip for cultivation of fish embryos and toxicity testing fabricated by 3D printing technology

RSC Advances ◽

10.1039/d1ra00846c ◽

2021 ◽

Vol 11 (33) ◽

pp. 20507-20518

Author(s):

Petr Panuška ◽

Zuzana Nejedlá ◽

Jiří Smejkal ◽

Petr Aubrecht ◽

Michaela Liegertová ◽

...

Keyword(s):

3D Printing ◽

Toxicity Testing ◽

Toxicity Screening ◽

Printing Technology ◽

3D Printing Technology ◽

Fish Embryos ◽

3D Printed ◽

Novel Design

A novel design of 3D printed zebrafish millifluidic system for embryonic long-term cultivation and toxicity screening has been developed. The chip unit provides 24 cultivation chambers and a selective individual embryo removal functionality.

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3D-Printed Nanocellulose-Based Cushioning–Antibacterial Dual-Function Food Packaging Aerogel

Molecules ◽

10.3390/molecules26123543 ◽

2021 ◽

Vol 26 (12) ◽

pp. 3543

Author(s):

Wei Zhou ◽

Jiawei Fang ◽

Shuwei Tang ◽

Zhengguo Wu ◽

Xiaoying Wang

Keyword(s):

3D Printing ◽

Food Packaging ◽

Fruits And Vegetables ◽

Antibacterial Effect ◽

Dual Function ◽

Printing Technology ◽

E Coli ◽

3D Printing Technology ◽

The Core ◽

3D Printed

Cushioning and antibacterial packaging are the requirements of the storage and transportation of fruits and vegetables, which are essential for reducing the irreversible quality loss during the process. Herein, the composite of carboxymethyl nanocellulose, glycerin, and acrylamide derivatives acted as the shell and chitosan/AgNPs were immobilized in the core by using coaxial 3D-printing technology. Thus, the 3D-printed cushioning–antibacterial dual-function packaging aerogel with a shell–core structure (CNGA/C–AgNPs) was obtained. The CNGA/C–AgNPs packaging aerogel had good cushioning and resilience performance, and the average compression resilience rate was more than 90%. Although AgNPs was slowly released, CNGA/C–AgNPs packaging aerogel had an obvious antibacterial effect on E. coli and S. aureus. Moreover, the CNGA/C–AgNPs packaging aerogel was biodegradable. Due to the customization capabilities of 3D-printing technology, the prepared packaging aerogel can be adapted to more application scenarios by accurately designing and regulating the microstructure of aerogels, which provides a new idea for the development of food intelligent packaging.

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Zn-Containing Membranes for Guided Bone Regeneration in Dentistry

Polymers ◽

10.3390/polym13111797 ◽

2021 ◽

Vol 13 (11) ◽

pp. 1797

Author(s):

Manuel Toledano ◽

Marta Vallecillo-Rivas ◽

María T. Osorio ◽

Esther Muñoz-Soto ◽

Manuel Toledano-Osorio ◽

...

Keyword(s):

Mechanical Properties ◽

Bone Regeneration ◽

Bone Healing ◽

Bone Repair ◽

Complex Modulus ◽

Bacterial Colonization ◽

Guided Bone Regeneration ◽

M2 Macrophage

Barrier membranes are employed in guided bone regeneration (GBR) to facilitate bone in-growth. A bioactive and biomimetic Zn-doped membrane with the ability to participate in bone healing and regeneration is necessary. The aim of the present study is to state the effect of doping the membranes for GBR with zinc compounds in the improvement of bone regeneration. A literature search was conducted using electronic databases, such as PubMed, MEDLINE, DIMDI, Embase, Scopus and Web of Science. A narrative exploratory review was undertaken, focusing on the antibacterial effects, physicochemical and biological properties of Zn-loaded membranes. Bioactivity, bone formation and cytotoxicity were analyzed. Microstructure and mechanical properties of these membranes were also determined. Zn-doped membranes have inhibited in vivo and in vitro bacterial colonization. Zn-alloy and Zn-doped membranes attained good biocompatibility and were found to be non-toxic to cells. The Zn-doped matrices showed feasible mechanical properties, such as flexibility, strength, complex modulus and tan delta. Zn incorporation in polymeric membranes provided the highest regenerative efficiency for bone healing in experimental animals, potentiating osteogenesis, angiogenesis, biological activity and a balanced remodeling. Zn-loaded membranes doped with SiO2 nanoparticles have performed as bioactive modulators provoking an M2 macrophage increase and are a potential biomaterial for promoting bone repair. Zn-doped membranes have promoted pro-healing phenotypes.

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APPLICATION OF 3D PRINTING TECHNOLOGY FOR RAPID TOOLING IN SHEET METAL FORMING

Fundamental and Applied Problems of Engineering and Technology ◽

10.33979/2073-7408-2020-341-3-20-30 ◽

2020 ◽

Vol 3 ◽

pp. 20-30

Author(s):

M.A. SEREZHKIN ◽

D.O. KLIMYUK ◽

A.I. PLOKHIKH

Keyword(s):

3D Printing ◽

Sheet Metal ◽

Sheet Metal Forming ◽

Metal Forming ◽

Rapid Tooling ◽

Printing Technology ◽

3D Printing Technology ◽

3D Printed ◽

Printed Materials ◽

Printing Parameters

The article presents the study of the application of 3D printing technology for rapid tooling in sheet metal forming for custom or small–lot manufacturing. The main issue of the usage of 3D printing technology for die tooling was discovered. It is proposed to use the method of mathematical modelling to investigate how the printing parameters affect the compressive strength of FDM 3D–printed parts. Using expert research methods, the printing parameters most strongly affecting the strength of products were identified for further experiments. A method for testing the strength of 3D–printed materials has been developed and tested.

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Implementation of 3D Printing Technology in the Food Industry

Scientific Review ◽

10.32861/sr.74.50.54 ◽

2021 ◽

pp. 50-54

Author(s):

Nor Aiman Sukindar ◽

Noorazizi Mohd Samsuddin ◽

Sharifah Imihezri Bt. Syed Shaharuddin ◽

Shafie Kamaruddin ◽

Ahmad Zahirani Ahmad Azhar ◽

...

Keyword(s):

3D Printing ◽

New Product Development ◽

Food Industry ◽

Printing Technology ◽

3D Printing Technology ◽

Fabrication Cost ◽

Food Packages ◽

3D Printed ◽

The Cost ◽

Fabrication Time

This project involves the implementation of 3D printing technology on designing and fabricating food holders in the food industry. Food holders are designed to hold the food packages in the filling line for food manufacturing industries that apply retort technology. Therefore, this study aims to implement the 3D printing technology in particular FDM to fabricate food holders for the food processing industry. The approach of using this technology is focused on giving more view on the capability of 3D printing technology, aiming at reducing the overall process fabrication cost and fabrication time. Hence, the fabrication cost and time between FDM and conventional machining methods were compared. This study revealed that Organic Gain food industry was able to reduce the cost and fabrication time for the food holder up to approximately 96.3% and 72% respectively. This project gives an insight into the ability of 3D printing technology in delivering the demands of the industry in producing parts as well as the adaptability of the technology to the industry in new product development. The project was carried out successfully and the 3D printed food holder has been tested and functions smoothly.

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Promising Applications of Additive-Manufactured (3D-printed) Electrochemical Sensors for Forensic Chemistry

Brazilian Journal of Analytical Chemistry ◽

10.30744/brjac.2179-3425.rv-50-2021 ◽

2021 ◽

Author(s):

Sílvia Castro ◽

Raquel Rocha ◽

Afonso João ◽

Eduardo Richter ◽

Rodrigo Munoz

Keyword(s):

3D Printing ◽

Illicit Drugs ◽

Industrial Revolution ◽

Electrochemical Sensors ◽

Forensic Chemistry ◽

Recent Contribution ◽

Printing Technology ◽

3D Printing Technology ◽

Wide Range ◽

3D Printed

Additive-manufacturing is one of the major pillars of the new industrial revolution and the three-dimensional (3D) printing technology has been highlighted in this scenario. Among the many areas benefited by 3D-printing, the development of electrochemical sensors has appeared in evidence in the last years. One potential application of 3D-printed electrochemical sensors is devoted to forensic chemistry, which demands for portable analytical methods that can provide on-site measurements and thus bring a relevant information in loco. In this context, this review highlights the recent contribution of 3D-printing technology on the development of electrochemical sensors with great promises for on-site analysis in “real-world” forensic scenarios. From the detection of trace explosives, gunshot residues, illicit drugs and chemical threats, to the measurement of adulterants in food and fuels, we show the wide range of applications that 3D-printed electrochemical sensors have been proposed and future demands that can be addressed by such a powerful, affordable, and accessible tool.

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3D Printing Technology in Drug Delivery: Recent Progress and Application

Current Pharmaceutical Design ◽

10.2174/1381612825666181206123828 ◽

2019 ◽

Vol 24 (42) ◽

pp. 5039-5048 ◽

Cited By ~ 13

Author(s):

Sabna Kotta ◽

Anroop Nair ◽

Nimer Alsabeelah

Keyword(s):

Drug Delivery ◽

3D Printing ◽

Drug Delivery Systems ◽

Delivery Systems ◽

Pharmaceutical Manufacturing ◽

Layer By Layer ◽

Printing Technology ◽

Drug Dosing ◽

3D Printing Technology ◽

3D Printed

Background: 3D printing technology is a new chapter in pharmaceutical manufacturing and has gained vast interest in the recent past as it offers significant advantages over traditional pharmaceutical processes. Advances in technologies can lead to the design of suitable 3D printing device capable of producing formulations with intended drug release. Methods: This review summarizes the applications of 3D printing technology in various drug delivery systems. The applications are well arranged in different sections like uses in personalized drug dosing, complex drugrelease profiles, personalized topical treatment devices, novel dosage forms and drug delivery devices and 3D printed polypills. Results: This niche technology seems to be a transformative tool with more flexibility in pharmaceutical manufacturing. Typically, 3D printing is a layer-by-layer process having the ability to fabricate 3D formulations by depositing the product components by digital control. This additive manufacturing process can provide tailored and individualized dosing for treatment of patients different backgrounds with varied customs and metabolism pattern. In addition, this printing technology has the capacity for dispensing low volumes with accuracy along with accurate spatial control for customized drug delivery. After the FDA approval of first 3D printed tablet Spritam, the 3D printing technology is extensively explored in the arena of drug delivery. Conclusion: There is enormous scope for this promising technology in designing various delivery systems and provides customized patient-compatible formulations with polypills. The future of this technology will rely on its prospective to provide 3D printing systems capable of manufacturing personalized doses. In nutshell, the 3D approach is likely to revolutionize drug delivery systems to a new level, though need time to evolve.

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