scholarly journals Standardized ileal digestible amino acids and net energy contents in full fat and defatted black soldier fly larvae meals (Hermetia illucens) fed to growing pigs

2020 ◽  
Vol 4 (3) ◽  
Author(s):  
Michelina Crosbie ◽  
Cuilan Zhu ◽  
Anna K Shoveller ◽  
Lee-Anne Huber
Keyword(s):  
Amino Acids ◽  
Body Weight ◽  
Latin Square ◽  
Growing Pigs ◽  
Net Energy ◽  
Initial Body Weight ◽  
Black Soldier Fly ◽  
Digestible Energy ◽  
Black Soldier Fly Larvae ◽  
Fly Larvae

Abstract Two experiments were conducted to determine standardized ileal digestibility (SID) of amino acids (AA; Exp. 1) and net energy (Exp. 2) in two black soldier fly larvae meal (BSFLM) samples [full fat (FF; 42.5% crude protein (CP), as-fed) and defatted (DF; 40.8% CP; as-fed)] for growing pigs. Two cornstarch-based diets were formulated with FF and DF BSFLM as the sole sources of AA. A nitrogen-free diet was also used, and the corn starch:sucrose:oil ratio was kept constant among diets to calculate digestible energy (DE) by difference method. In each experiment, pigs were fed 2.8 × estimated maintenance energy requirement. In Exp. 1, eight ileal-cannulated barrows (25.1 ± 0.41 kg initial body weight) were used in a replicated 2 × 2 Latin square design (n = 8). In each period, pigs were adapted to diets for 5 d followed by 2 d of continuous ileal digesta collection for 8 h. The SID of AA were calculated using basal endogenous losses for pigs fed a nitrogen-free diet. In Exp. 2, eight barrows [23.4 ± 0.54 kg initial body weight (BW)] were used in a partially replicated Latin square design (n = 8). In each period, pigs were adapted to diets for 7 d, followed by 5 d of total urine collection and fecal grab sampling. The SID of CP (80.6 ± 1.1%) and Lys (88.0 ± 1.4%) were not different between FF and DF BSFLM. The SID of Arg, Val, Ala, and Pro tended to be less, and the SID of Met tended to be greater for the FF versus the DF BSFLM (P = 0.034, 0.090, 0.053, 0.065, 0.074, respectively). Digestible energy (4,927 vs. 3,941 ± 75 kcal/kg), metabolizable energy (4,569 vs. 3,396 ± 102 kcal/kg), and predicted net energy (3,477 vs. 2,640 ± 30 kcal/kg, using equations from Noblet; 3,479 vs. 2,287 ± 28 kcal/kg, using equations from Blok, respectively) were greater for the FF versus the DF BSFLM (P < 0.05). The apparent total tract digestibility of neutral detergent fiber and acid detergent fiber were greater for the FF versus the DF BSFLM (P ≤ 0.05). Both FF and DF BSFLM had high SID for most AA; however, FF BSFLM was a better source of net energy for growing pigs. Therefore, both FF and DF BSFLM could be used as protein alternatives in growing pig diets.

scholarly journals 169 Standardized ileal digestible amino acids and net energy contents in full fat and defatted black soldier fly larvae meals (Hermatia illucens) fed to growing pigs

2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 63-63
Author(s):  
Michelina M Crosbie ◽  
Cuilan Zhu ◽  
Anna Kate Shoveller ◽  
Lee-Anne Huber
Keyword(s):  
Corn Starch ◽  
Latin Square ◽  
Growing Pigs ◽  
Neutral Detergent Fiber ◽  
Net Energy ◽  
Sample Collection ◽  
Black Soldier Fly ◽  
Available Energy ◽  
Black Soldier Fly Larvae ◽  
Fly Larvae

Abstract Two experiments were conducted to determine the standardized ileal digestibility (SID) of AA (Exp. 1) and available energy (Exp. 2) in two black soldier fly larvae meal (BSFLM) sources [full fat (FF) and defatted (DF)] for growing pigs. Two corn starch-based diets were formulated with FF and DF BSFLM as the sole sources of AA. A nitrogen-free diet was also used and the corn starch:sucrose:oil ratio was kept constant among diets to calculate digestible energy. In Exp. 1, 8 ileal-cannulated barrows (25.1±0.41 kg initial BW) were used in a replicated 2 x 2 Latin square design (n = 8). In each period, pigs were adjusted to diets for 5d followed by 2d of continuous ileal digesta collection for 8 h. The SID of AA were calculated using basal endogenous losses for pigs fed a nitrogen-free diet. In Exp. 2, 8 barrows (23.4±0.54 kg initial BW) were used in a partially replicated Latin square design (n=8). In each period, pigs were adjusted to diets for 7d, followed by 5d of total urine and fecal grab sample collection, and 24hr in indirect calorimetry chambers to determine heat production. The SID of Arg, Val, Ala, and Pro tended to be less, and the SID of Met tended to be greater for the FF versus the DF BSFLM (P=0.034, 0.090, 0.053, 0.065, 0.074, respectively). Digestible, metabolizable, and net energy (4101 vs 3257±224 kcal/kg) were greater for the FF versus the DF BSFLM (P< 0.05). The ATTD of phosphorus, neutral detergent fiber, and acid detergent fiber were greater for the FF versus the DF BSFLM (P< 0.05). In conclusion, the SID AA in FF and DF BSFLM were high and similar to those of soybean meal. The FF BSFLM is a better source of available energy for growing pigs compared to DF BSFLM.

Digestibility of amino acids in pigs fed distillers dried grains with solubles derived from corn, sorghum, and triticale

2020 ◽  
Author(s):  
Chan Sol Park ◽  
Darryl Ragland ◽  
O. Adeola
Keyword(s):  
Amino Acids ◽  
Body Weight ◽  
Latin Square ◽  
Experimental Period ◽  
Growing Pigs ◽  
Initial Body Weight ◽  
Isoleucine Leucine ◽  
Ileal Digestibility ◽  
Distillers Dried Grains ◽  
Dietary Treatments

An experiment was conducted to determine the standardized ileal digestibility (SID) of amino acids (AA) in distillers dried grains with solubles derived from corn (C-DDGS), sorghum (S-DDGS), and triticale (T-DDGS) fed to growing pigs. A total of 16 barrows (initial body weight = 23.1 ± 2.03 kg) surgically fitted with T-cannulas at the distal ileum were divided into 4 groups based on body weight and assigned to a quadruplicated 4 × 2 incomplete Latin Square design with 4 dietary treatments and 2 experimental periods. Three diets containing C-DDGS, S-DDGS, or T-DDGS and a nitrogen-free diet were fed in each experimental period consisting of 5-d adaptation and 2-d ileal digesta collection. Pigs fed S-DDGS had less (P < 0.05) SID of histidine, isoleucine, leucine, and methionine compared with those fed T-DDGS, which were less (P < 0.05) than the values observed from pigs fed C-DDGS. The SID of arginine, phenylalanine, threonine, and valine in C-DDGS were greater (P < 0.05) than in S-DDGS but were not different from those in T-DDGS. The SID of lysine in C-DDGS was greater (P < 0.05) than in S-DDGS and T-DDGS. In conclusion, AA in S-DDGS were less digestible than those in C-DDGS and T-DDGS.

141 Relationship Between Dietary Fiber and Basal Ileal Endogenous Losses of Amino Acids in Growing Pigs

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 72-72
Author(s):  
Chan Sol Park ◽  
Darryl Ragland ◽  
Olayiwola Adeola
Keyword(s):  
Amino Acids ◽  
Body Weight ◽  
Dietary Fiber ◽  
Latin Square ◽  
Sole Source ◽  
Growing Pigs ◽  
The Other ◽  
Canola Meal ◽  
Initial Body Weight ◽  
Ileal Digestibility

Abstract Two experiments were conducted to determine the influence of sources or concentrations of dietary fiber in nitrogen-free diets (NFD) on basal ileal endogenous losses (BEL) of amino acids (AA) and subsequent standardized ileal digestibility (SID) of AA in feed ingredients for pigs derived from such BEL. In Exp. 1, 20 pigs (initial body weight = 46.5 ± 2.97 kg) were assigned to five diets in a quadruplicate 5 × 2 incomplete Latin square design with two periods. Three NFD containing cellulose (insoluble and fermentable fiber), inulin (soluble and fermentable fiber), or carboxymethyl cellulose (CMC; soluble and non-fermentable fiber) at 40 g/kg plus two diets containing soybean meal (SBM) or wheat distillers’ dried grains (WDDG) as the sole source of nitrogen were prepared. Pigs fed NFD containing CMC had greater (P &lt; 0.05) BEL of AA, except for Arg and Pro, than those fed the other NFD. The SID of most AA in SBM and WDDG corrected by BEL of AA from NFD containing CMC were greater (P &lt; 0.05) than those corrected by BEL of AA from the other NFD. Experiment 2 was conducted with 21 barrows (initial body weight = 34.1 ± 2.57 kg) assigned to seven diets in a triplicate 7 × 3 incomplete Latin square design with 3 periods. Seven diets consisted of three NFD containing 4, 8, or 12 g/kg cellulose and four diets containing corn, wheat, SBM, or canola meal as the sole source of nitrogen. There was no difference in the BEL of AA, except for Pro, among pigs fed NFD with increasing concentration of cellulose. The SID of AA in test ingredients were not affected by BEL of AA from NFD containing increasing concentration of cellulose. In conclusion, chemical characteristics of dietary fiber, but not concentration, affect the BEL of AA in pigs.

scholarly journals Energy, amino acid, and phosphorus digestibility and energy prediction of thermally processed food waste sources for swine1

2019 ◽  
Vol 3 (2) ◽  
pp. 676-691 ◽  
Author(s):  
Leonard Fung ◽  
Pedro E Urriola ◽  
Gerald C Shurson
Keyword(s):  
Amino Acids ◽  
Body Weight ◽  
Food Waste ◽  
Block Design ◽  
Growing Pigs ◽  
In Vitro Digestibility ◽  
Prediction Equations ◽  
Initial Body Weight

Abstract Recycling energy and nutrients from food waste into animal feed decreases the environmental impact of food animal production. However, recycling energy and nutrients from various food waste sources into swine feeding programs is constrained by the high variability and lack of data on the digestibility of energy and nutrients. Therefore, the objectives of this study were to evaluate the digestibility of energy, amino acids, and phosphorus in thermally dried food waste sources fed to growing pigs and to compare in vivo determined digestibility values with those obtained from in vitro digestibility procedures and published prediction equations to determine the accuracy of using these nutritional evaluation methods. Pigs (n = 36; initial body weight = 16.37 ± 1.9 kg) were utilized to determine digestible energy (DE) and metabolizable energy (ME) content, as well as standardized total tract digestibility (STTD) of phosphorus and standardized ileal digestibility (SID) of amino acids in three sources of dehydrated food waste in three separate trials. Initial body weight of pigs at the beginning of each digestibility trial was used as the blocking factor in a randomized complete block design. Diets were formulated to contain 30% food waste derived from fish waste (FW), supermarket waste (containing bakery, fruits and vegetables, meat, and deli foods from a single supermarket; SMW), and fruit and vegetable waste (FVW). The DE and ME content of FW (DE = 5,057 kcal/kg; ME = 4,820 kcal/kg) and SMW (DE = 5,071 kcal/kg; ME = 4,922 kcal/kg) were not different (P &gt; 0.05), whereas FVW had the least (P &lt; 0.05) DE (2,570 kcal/kg) and ME (2,460 kcal/kg) content compared with FW and SMW. Digestibility of crude protein and amino acids was greater (P &lt; 0.05) in FW and SMW compared with FVW. The in vitro digestibility procedure can be used to approximate the digestibility of dry matter (DM) and energy in SMW, FW, and FVW compared with in vivo estimates, but significant error exists depending on the chemical characteristics of each food waste source. However, use of the prediction equations and digestibility data obtained from the in vitro procedure resulted in high accuracy in estimating DE content of FW (observed = 5,058 kcal/kg DM vs. predicted = 4,948 kcal/kg DM), SMW (observed = 5,071 kcal/kg DM vs. predicted 4,978 kcal/kg DM), and FVW (observed = 2,570 kcal/kg DM vs. predicted 2,814 kcal/kg DM) sources.

scholarly journals Digestibility of amino acids, energy, acid hydrolyzed ether extract, and neutral detergent fiber, and concentration of digestible and metabolizable energy in low-oil distillers dried grains with solubles fed to growing pigs1

2019 ◽  
Vol 3 (2) ◽  
pp. 662-675 ◽  
Author(s):  
Charmaine D Espinosa ◽  
Su A Lee ◽  
Hans H Stein
Keyword(s):  
Amino Acids ◽  
Body Weight ◽  
Latin Square ◽  
Basal Diet ◽  
Metabolizable Energy ◽  
Neutral Detergent Fiber ◽  
Ether Extract ◽  
Initial Body Weight ◽  
Distillers Dried Grains ◽  
Gross Energy

Abstract Two experiments were conducted to test the hypothesis that digestibility of amino acids (AA), gross energy (GE), acid hydrolyzed ether extract (AEE), and neutral detergent fiber (NDF), and values for metabolizable energy (ME) in low-oil distillers dried grains with solubles (DDGS) vary among suppliers. In Exp. 1, the apparent total tract digestibility (ATTD) of GE, AEE, and NDF, and concentration of ME were determined in eight sources of DDGS (sources A, B, C, D, E, G, H, and I). A corn-based basal diet and eight diets containing corn and each source of DDGS were fed to 72 barrows (initial body weight = 18.1 ± 1.3 kg) with eight pigs per diet. Feces and urine were collected for 5 d after 7 d of adaptation. The ME did not differ among the eight sources of DDGS with the exception that DDGS source E contained less (P &lt; 0.05) ME than DDGS source D. The ATTD of GE did also not differ among the eight sources of DDGS, but ME and ATTD of GE in corn were greater (P &lt; 0.05) than in the eight sources of DDGS. However, the ATTD of AEE in corn and the eight sources of DDGS was not different, but the ATTD of AEE in DDGS source E was greater (P &lt; 0.05) than in DDGS source A. The ATTD of NDF in DDGS source D was also greater (P &lt; 0.05) than in DDGS sources E, G, and H, but ATTD of NDF did not differ between corn and the eight sources of DDGS. In Exp. 2, standardized ileal digestibility (SID) of AA was determined in seven sources of DDGS (sources A, B, C, D, E, G, and H). Twenty-four barrows (initial body weight = 63.4 ± 3.4 kg) that had a T-cannula installed in the distal ileum were allotted to a two-period incomplete Latin square design with eight diets. Seven diets were formulated to contain each of the seven sources of DDGS and an N-free diet was also used. Ileal digesta were collected for 2 d after 5 d of adaptation. There were no differences between pigs fed DDGS sources A and B in SID of AA, and the SID of Lys, Met, and Trp did not differ among DDGS sources A, B, and E. However, SID of most indispensable and dispensable AA except Gly were greater (P &lt; 0.05) in DDGS source B than in DDGS sources C, D, E, G, and H. In conclusion, variability in SID of AA, ATTD of NDF and AEE, and ME were observed among the sources of DDGS used in this experiment.

scholarly journals PSXI-28 Reducing dietary crude protein and supplementation with amino acids helps mitigate the environmental impacts of growing pigs production determined by the life cycle assessment

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 383-383
Author(s):  
Lucas Antonio C Esteves ◽  
Natália Y Sitanaka ◽  
Alessandra N T R Monteiro ◽  
Suelen M Einsfeld ◽  
Lucas P Bonagurio ◽  
...  
Keyword(s):  
Amino Acids ◽  
Body Weight ◽  
Life Cycle Assessment ◽  
Environmental Impacts ◽  
Crude Protein ◽  
Block Design ◽  
Growing Pigs ◽  
Experimental Unit ◽  
Initial Body Weight ◽  
Randomized Block Design

Abstract This study aimed to evaluate, through life cycle assessment and using experimental data, the environmental impacts of growing pigs’ production (30 to 50 kg) fed diets with reduced crude protein (CP) levels, and supplemented with industrial amino acids. Experiment I (nitrogen and phosphorus balance): 20 crossbred barrows, averaging 31.80 ± 2.39 kg of initial body weight, were housed in metabolic cages and allotted in a randomized block design with four treatments, five replicates and one animal per experimental unit. Experiment II (performance): 44 crossbred barrows, averaging 30.10 ± 0.63 kg of initial body weight, were housed in a randomized block design, with four treatments, 11 replicates and one animal per experimental unit. The treatments for both experiments consisted of four diets containing 18.15, 17.15, 16.15 and 15.15% of CP, and supplemented with industrial amino acids, in order to meet the pig’s requirements of SID amino acids. According to the obtained data (Experiments I and II), the environmental impacts for global warming potential (GWP), acidification potential (AC), eutrophication potential (EU), cumulative energy demand (CED), terrestrial ecotoxicity (TE) and land occupation (LO), per kg of body weight gain, were calculated (CML 2001 method). There was a reduction in some studied environmental impacts as the dietary CP reduced from 18.15 to 15.15%, observing a reduction in the AC from 35.34 to 31.58 g SO2-eq (P = 0.015), there was also a reduction in the EU from 11.90 to 10.31 g PO4-eq (P = 0.001), and the LO was reduced from 2.15 to 1.89 m2-year (P = 0.005), respectively. GWP, CED and TE were not affected (P &gt; 0.05) by the dietary CP reduction, although some variations were observed. It was concluded that the dietary CP reduction, supplementing with industrial amino acids, may help to mitigate the environmental impacts caused by growing pig production for the AC, EU and LO categories.

scholarly journals Standardized ileal digestible amino acids and apparent metabolizable energy content in defatted black soldier fly larvae meal fed to broiler chickens

2019 ◽  
Vol 99 (2) ◽  
pp. 211-217 ◽  
Author(s):  
Z.N. Mwaniki ◽  
E. Kiarie
Keyword(s):  
Amino Acids ◽  
Broiler Chickens ◽  
Energy Content ◽  
Metabolizable Energy ◽  
Broiler Chicks ◽  
Test Diet ◽  
Black Soldier Fly ◽  
Apparent Metabolizable Energy ◽  
Black Soldier Fly Larvae ◽  
Fly Larvae

Standardized ileal digestibility (SID) of amino acids (AA) and apparent metabolizable energy corrected for nitrogen (AMEn) in defatted black soldier fly larvae meal (BSFLM) were determined in broiler chicks. A total of 180-d-old male broiler chicks (Ross 708) were fed a commercial broiler starter diet to day 13 of age. On day 14, birds were weighed and placed in cages (10 birds per cage; n = 6) and allocated one of two semi-purified cornstarch-based diets. The diets were nitrogen-free diet (NFD) for estimating endogenous AA losses and 20% crude protein test diet with BSFLM as the sole source of AA. All diets had 0.5% titanium dioxide (TiO2) as an indigestible marker and the ratio of cornstarch to sucrose and soy oil in the test diet was identical to NFD to calculate AMEn by difference method. Birds were given feed and water ad libitum. Excreta samples were collected on days 17–20 and ileal digesta on day 21. The SID of lysine, methionine, cysteine, threonine, isoleucine, and valine was 86.3%, 88.7%, 72.8%, 85.5%, 89.6%, and 88.6%, respectively. Apparent retention of gross energy in BSFLM was 64.5% ± 2.27% and AMEn was 2902 ± 101 kcal kg−1 dry matter. The data will aid in accurate incorporation of BSFLM in poultry feeding programs.

scholarly journals The results of experimental studies of extrusion of feed containing wheat grain and black soldier fly larvae biomass

2020 ◽  
Vol 21 (1) ◽  
pp. 28-42 ◽  
Author(s):  
V. I. Pakhomov ◽  
S. V. Braginets ◽  
O. N. Bakhchevnikov ◽  
A. S. Alferov ◽  
A. I. Rukhlyada ◽  
...  
Keyword(s):  
Amino Acids ◽  
Experimental Studies ◽  
Extrusion Temperature ◽  
Wheat Grain ◽  
Feed Mixture ◽  
Black Soldier Fly ◽  
Indispensable Amino Acids ◽  
Black Soldier Fly Larvae ◽  
Fly Larvae ◽  
Extruded Feed

The experimental studies were carried out in the Rostov region of Russia in 2019. Three variants of grinded wheat grain mixed with biomass of black soldier fly larvae with raw animal material content of 10, 12.5 and 15 % by mass were extruded at varying temperature. The content of amino acids in raw material and finished extrudate was determined. It has been established that the feed mixture containing grinded wheat grain and biomass of black soldier fly larvae can be successfully extruded at the temperature of 121-135 ºC. As the extrusion temperature rises in the range of 115º-140 ºС, the content of amino acids in a finished extrudate decreases. Change in larvae content in the feed mixture does not affect the nature of the correlation between the amino acids content in the extrudate and the extrusion temperature and process behavior. It has been found that with increasing temperature the amino acids content in the extruded feed decreases with an accelerated velocity irrespective of the insect biomass content. The rational temperature range for extrusion of feed mixture containing grinded wheat grain and larvae biomass was determined within 121-127 ºC. This range provides no more than 30 % decrease in indispensable amino acids content in the extrudate. The extruded feed containing 15% of insect larvae biomass contains 9.6±0.13 % of amino acids, including 4.38±2.01 % of indispensable amino acids. Extrusion of larvae biomass mixed with grain of cereal crops is considered to be a promising direction for improving production of feed for fish and farm animals.

Live black soldier fly larvae (Hermetia illucens) for turkey poults

2019 ◽  
Vol 5 (4) ◽  
pp. 301-311 ◽  
Author(s):  
T. Veldkamp ◽  
T.G.C.M. van Niekerk
Keyword(s):  
Body Weight ◽  
Feed Intake ◽  
Skin Damage ◽  
Control Groups ◽  
Black Soldier Fly ◽  
Hermetia Illucens ◽  
Severe Problem ◽  
Natural Behaviour ◽  
Black Soldier Fly Larvae ◽  
Fly Larvae

Black soldier fly larvae (BSFL; Hermetia illucens) are a suitable protein source for poultry. However the effect of live BSFL supplementation on growth performance and behaviour has never been demonstrated and quantified in turkeys. Wild turkeys eat insects during the first two weeks of life which is in contrast with commercially fed crumbs or pellets. Damaging pecking behaviour is a severe problem in turkeys. More lively diets may improve natural behaviour and decrease damaging pecking behaviour. The aim of the experiment was to stimulate natural behaviour of young non-beak treated turkeys by supply of live BSFL to avoid damaging pecking behaviour. Two treatments with seven replicates were studied in 14 floor pens (1.5 m2/pen and 20 turkeys per pen) from 0 to 35 days of age. Control groups were fed commercial diets and BSFL groups received live BSFL. The daily BSFL intake was calculated to be 10% of the expected daily feed intake (based on fresh weight) and dietary nutrient composition was adjusted in a way that control and BSFL groups were fed iso-nutritious. Daily feed intake and body weight gain of BSFL groups were significantly higher compared to control groups resulting in a significantly higher body weight at five weeks of age (2,190 vs 2,015 g; P=0.003) and a significantly lower feed conversion ratio. In the first week there was a tendency for more foraging related behaviour for the BSFL groups, but in the third and fifth week BSFL groups showed less foraging related behaviour compared to control groups. Provision of BSFL reduced aggressive pecking directed at the back and tail base at 5 weeks of age. Although the incidence of feather and skin damage was low, this type of damage tended to be lower in the BSFL groups.

DETERMINATION OF THE METHIONINE REQUIREMENT OF GROWING PIGS USING SERUM FREE AMINO ACIDS

1972 ◽  
Vol 52 (1) ◽  
pp. 163-169 ◽  
Author(s):  
M. O. KEITH ◽  
D. A. CHRISTENSEN ◽  
B. D. OWEN
Keyword(s):  
Amino Acids ◽  
Body Weight ◽  
Free Amino Acids ◽  
Free Amino ◽  
Latin Square ◽  
Growing Pigs ◽  
Performance Data ◽  
Serum Free ◽  
Methionine Concentration

Six littermate Yorkshire gilts averaging 18 kg body weight were fed a semipurified diet containing six graded levels of methionine during six 4-day feeding periods in a Latin square design. Serum methionine concentrations were determined at the end of each period. Plotting serum methionine concentration against dietary methionine intake showed the methionine requirement to be 0.46% of the diet; this estimate was substantiated by animal performance data.

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