scholarly journals Digestibility of amino acids, energy, acid hydrolyzed ether extract, and neutral detergent fiber, and concentration of digestible and metabolizable energy in low-oil distillers dried grains with solubles fed to growing pigs1

2019 ◽  
Vol 3 (2) ◽  
pp. 662-675 ◽  
Author(s):  
Charmaine D Espinosa ◽  
Su A Lee ◽  
Hans H Stein

Abstract Two experiments were conducted to test the hypothesis that digestibility of amino acids (AA), gross energy (GE), acid hydrolyzed ether extract (AEE), and neutral detergent fiber (NDF), and values for metabolizable energy (ME) in low-oil distillers dried grains with solubles (DDGS) vary among suppliers. In Exp. 1, the apparent total tract digestibility (ATTD) of GE, AEE, and NDF, and concentration of ME were determined in eight sources of DDGS (sources A, B, C, D, E, G, H, and I). A corn-based basal diet and eight diets containing corn and each source of DDGS were fed to 72 barrows (initial body weight = 18.1 ± 1.3 kg) with eight pigs per diet. Feces and urine were collected for 5 d after 7 d of adaptation. The ME did not differ among the eight sources of DDGS with the exception that DDGS source E contained less (P < 0.05) ME than DDGS source D. The ATTD of GE did also not differ among the eight sources of DDGS, but ME and ATTD of GE in corn were greater (P < 0.05) than in the eight sources of DDGS. However, the ATTD of AEE in corn and the eight sources of DDGS was not different, but the ATTD of AEE in DDGS source E was greater (P < 0.05) than in DDGS source A. The ATTD of NDF in DDGS source D was also greater (P < 0.05) than in DDGS sources E, G, and H, but ATTD of NDF did not differ between corn and the eight sources of DDGS. In Exp. 2, standardized ileal digestibility (SID) of AA was determined in seven sources of DDGS (sources A, B, C, D, E, G, and H). Twenty-four barrows (initial body weight = 63.4 ± 3.4 kg) that had a T-cannula installed in the distal ileum were allotted to a two-period incomplete Latin square design with eight diets. Seven diets were formulated to contain each of the seven sources of DDGS and an N-free diet was also used. Ileal digesta were collected for 2 d after 5 d of adaptation. There were no differences between pigs fed DDGS sources A and B in SID of AA, and the SID of Lys, Met, and Trp did not differ among DDGS sources A, B, and E. However, SID of most indispensable and dispensable AA except Gly were greater (P < 0.05) in DDGS source B than in DDGS sources C, D, E, G, and H. In conclusion, variability in SID of AA, ATTD of NDF and AEE, and ME were observed among the sources of DDGS used in this experiment.

2020 ◽  
Vol 98 (10) ◽  
Author(s):  
Diego A Rodriguez ◽  
Su A Lee ◽  
Hans H Stein

Abstract Two experiments were conducted to test the hypothesis that the digestibility of gross energy (GE) and nutrients, and concentrations of digestible energy (DE) and metabolizable energy (ME) in two sources of distillers dried grains with solubles (DDGS) are not different despite different concentrations of fat in the two sources. Cold-fermented DDGS (6.82% fat) and a conventional DDGS (9.54% fat) were used. In experiment 1, 12 growing barrows (initial body weight = 55.2 ± 3.6 kg) that had a T-cannula installed in the distal ileum were allotted to one of three diets and two periods. Two diets contained either cold-fermented or conventional DDGS as the sole source of crude protein (CP) and amino acids (AA). The third diet was an N-free diet that was used to determine the basal endogenous losses of AA from the pigs. Each experimental period lasted 7 d and ileal digesta were collected on days 6 and 7 of each period. Results demonstrated that values for the standardized ileal digestibility (SID) of CP and most AA were greater (P < 0.05) or tended to be greater (P < 0.10) in cold-fermented than in conventional DDGS. In experiment 2, 24 barrows (initial body weight = 17.3 ± 1.3 kg) were randomly allotted to three diets with 8 replicate pigs per diet. A corn-based basal diet and two diets containing corn and either cold-fermented DDGS or conventional DDGS were formulated. Pigs were housed individually in metabolism crates and feces and urine were collected separately for 5 d after 7 d of adaptation. The apparent total tract digestibility (ATTD) of neutral detergent fiber (NDF), acid detergent fiber (ADF), and acid-hydrolyzed ether extract (AEE) was greater (P < 0.01) in conventional DDGS than in cold-fermented DDGS, but there was no difference in ATTD of GE between the two sources of DDGS. However, conventional DDGS contained more (P < 0.001) DE and ME than cold-fermented DDGS because of greater GE. In conclusion, the SID of AA was greater in cold-fermented DDGS than in the conventional DDGS that was evaluated in this experiment, but the ATTD of NDF, ADF, and AEE, and ME were greater in conventional DDGS than in cold-fermented DDGS.


2020 ◽  
Vol 100 (4) ◽  
pp. 729-738
Author(s):  
Peili Li ◽  
Zhiqian Lyu ◽  
Lu Wang ◽  
Bingbing Huang ◽  
Changhua Lai

Two experiments were conducted to evaluate the effects of adding multi-enzyme on the available energy concentration and standardized ileal digestibility (SID) of amino acids of double-low rapeseed expellers (RSE) and rapeseed meal (RSM) fed to growing pigs. In Experiment 1, a total of 36 growing barrows were fed six diets in a replicated 3 × 2 factorial design. Diets included a corn–soybean meal basal diet, RSE diet, and RSM diet with or without multi-enzyme (1000 U g−1 of cellulase, 10 000 U g−1 of xylanase, 2000 U g−1 of glucanase, and 10 000 U g−1 of protease). In Experiment 2, a total of 30 crossbred pigs were randomly allotted to five diets consisting of a nitrogen-free diet and four cornstarch-based diets containing RSE or RSM with or without multi-enzyme. Multi-enzyme supplementation significantly improved (P < 0.05) the metabolizable energy (ME) value and apparent total tract digestibility (ATTD) of ether extract, neutral detergent fiber, and acid detergent fiber, and it tended to increase (P < 0.10) the ATTD of gross energy and digestible energy in ingredients. With the exception of phenylalanine, multi-enzyme supplementation increased (P < 0.05) the SID of crude protein (CP) and all amino acids (AA) in ingredients. Multi-enzyme supplementation enhanced fiber degradation and increased the ME value and SID of CP as well as most AA in double-low rapeseed co-products fed to pigs.


Animals ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 1168
Author(s):  
Lu Wang ◽  
Qile Hu ◽  
Peili Li ◽  
Changhua Lai ◽  
Defa Li ◽  
...  

The study was conducted to develop and validate an equation to predict the metabolizable energy (ME) of double-low rapeseed cakes (DLRSC) for growing pigs based on their chemical compositions. In Experiment 1, 66 growing pigs (initial body weight 36.6 ± 4.1 kg) were allotted randomly to a completely randomized design with 11 diets. The diets included a corn–soybean meal basal diet and 10 test diets containing 19.22% DLRSC supplemented at the expense of corn, soybean meal, and lysine. Neutral detergent fiber (NDF), crude fiber (CF), and gross energy (GE) were the best predictors to determine ME. The best-fit prediction equation of ME (MJ/kg) was ME = 9.33 − 0.09 × NDF − 0.25 × CF + 0.59 × GE (R2 = 0.93). In Experiment 2, a total of 144 growing pigs (initial body weight 29.7 ± 2.7 kg), with six pigs per pen and six pens per treatment, were assigned randomly to four treatments in a completely randomized block design for a 28-day feeding trial. A corn–soybean meal basal diet was prepared, and three additional diets were formulated by adding 7%, 14%, and 21% DLRSC to the basal diet at the expense of soybean meal. All diets were formulated to provide equal standardized ileal digestibility (SID) Lys/ME ratio and SID essential amino acids/SID Lys ratio. Increasing dietary levels of DLRSC had no effect on average daily feed intake, average daily gain, and feed-to-gain ratio. The caloric efficiency of ME (31.83, 32.44, 31.95, and 32.69 MJ/kg, respectively) was not changed by increasing the dietary concentration of DLRSC. Increasing dietary levels of DLRSC linearly reduced (p < 0.05) the concentrations of triiodothyronine and tetraiodothyronine in serum, as well as apparent total tract digestibility of DM, GE, crude protein, acid detergent fiber, and organic matter of the diet. In conclusion, the ME prediction equation obtained in Experiment 1 accurately estimates the ME value of DLRSC fed to growing pigs.


Author(s):  
Chan Sol Park ◽  
Darryl Ragland ◽  
O. Adeola

An experiment was conducted to determine the standardized ileal digestibility (SID) of amino acids (AA) in distillers dried grains with solubles derived from corn (C-DDGS), sorghum (S-DDGS), and triticale (T-DDGS) fed to growing pigs. A total of 16 barrows (initial body weight = 23.1 ± 2.03 kg) surgically fitted with T-cannulas at the distal ileum were divided into 4 groups based on body weight and assigned to a quadruplicated 4 × 2 incomplete Latin Square design with 4 dietary treatments and 2 experimental periods. Three diets containing C-DDGS, S-DDGS, or T-DDGS and a nitrogen-free diet were fed in each experimental period consisting of 5-d adaptation and 2-d ileal digesta collection. Pigs fed S-DDGS had less (P < 0.05) SID of histidine, isoleucine, leucine, and methionine compared with those fed T-DDGS, which were less (P < 0.05) than the values observed from pigs fed C-DDGS. The SID of arginine, phenylalanine, threonine, and valine in C-DDGS were greater (P < 0.05) than in S-DDGS but were not different from those in T-DDGS. The SID of lysine in C-DDGS was greater (P < 0.05) than in S-DDGS and T-DDGS. In conclusion, AA in S-DDGS were less digestible than those in C-DDGS and T-DDGS.


2020 ◽  
Vol 4 (3) ◽  
Author(s):  
Michelina Crosbie ◽  
Cuilan Zhu ◽  
Anna K Shoveller ◽  
Lee-Anne Huber

Abstract Two experiments were conducted to determine standardized ileal digestibility (SID) of amino acids (AA; Exp. 1) and net energy (Exp. 2) in two black soldier fly larvae meal (BSFLM) samples [full fat (FF; 42.5% crude protein (CP), as-fed) and defatted (DF; 40.8% CP; as-fed)] for growing pigs. Two cornstarch-based diets were formulated with FF and DF BSFLM as the sole sources of AA. A nitrogen-free diet was also used, and the corn starch:sucrose:oil ratio was kept constant among diets to calculate digestible energy (DE) by difference method. In each experiment, pigs were fed 2.8 × estimated maintenance energy requirement. In Exp. 1, eight ileal-cannulated barrows (25.1 ± 0.41 kg initial body weight) were used in a replicated 2 × 2 Latin square design (n = 8). In each period, pigs were adapted to diets for 5 d followed by 2 d of continuous ileal digesta collection for 8 h. The SID of AA were calculated using basal endogenous losses for pigs fed a nitrogen-free diet. In Exp. 2, eight barrows [23.4 ± 0.54 kg initial body weight (BW)] were used in a partially replicated Latin square design (n = 8). In each period, pigs were adapted to diets for 7 d, followed by 5 d of total urine collection and fecal grab sampling. The SID of CP (80.6 ± 1.1%) and Lys (88.0 ± 1.4%) were not different between FF and DF BSFLM. The SID of Arg, Val, Ala, and Pro tended to be less, and the SID of Met tended to be greater for the FF versus the DF BSFLM (P = 0.034, 0.090, 0.053, 0.065, 0.074, respectively). Digestible energy (4,927 vs. 3,941 ± 75 kcal/kg), metabolizable energy (4,569 vs. 3,396 ± 102 kcal/kg), and predicted net energy (3,477 vs. 2,640 ± 30 kcal/kg, using equations from Noblet; 3,479 vs. 2,287 ± 28 kcal/kg, using equations from Blok, respectively) were greater for the FF versus the DF BSFLM (P &lt; 0.05). The apparent total tract digestibility of neutral detergent fiber and acid detergent fiber were greater for the FF versus the DF BSFLM (P ≤ 0.05). Both FF and DF BSFLM had high SID for most AA; however, FF BSFLM was a better source of net energy for growing pigs. Therefore, both FF and DF BSFLM could be used as protein alternatives in growing pig diets.


2020 ◽  
Vol 33 (11) ◽  
pp. 1787-1796 ◽  
Author(s):  
Wenxuan Dong ◽  
Juntao Li ◽  
Zhongchao Li ◽  
Shuo Zhang ◽  
Xiaozhen Li ◽  
...  

Objective: The objective of this study was to determine the digestible energy (DE) and metabolizable energy (ME) of yellow dent corn sourced from different meteorological origins fed to growing pigs and develop equations to predict the DE and ME of yellow dent corn from southwestern China.Methods: Sixty crossbred barrows were allotted to 20 treatments in a triplicate 20×2 incomplete Latin square design with 3 replicated pigs per dietary treatment during 2 consecutive periods. Each period lasted for 12 days, and total feces and urine during the last 5 days of each period were collected to calculate the energy contents.Results: On dry matter (DM) basis, the DE and ME in 20 corn grain samples ranged from 15.38 to 16.78 MJ/kg and from 14.93 to 16.16 MJ/kg, respectively. Selected best-fit prediction equations for DE and ME (MJ/kg DM basis) for yellow dent corn (n = 16) sourced from southwestern China were as follows: DE = 28.58–(0.12×% hemicellulose)+(0.35×% ether extract)–(0.83×MJ/kg gross energy)+(0.20×% crude protein)+(0.49×% ash); ME = 30.42– (0.11×% hemicellulose)+(0.31×% ether extract)–(0.81×MJ/kg gross energy).Conclusion: Our results indicated that the chemical compositions, but not the meteorological conditions or physical characteristics could explain the variation of energy contents in yellow dent corn sourced from southwestern China fed to growing pigs.


2015 ◽  
Vol 36 (6Supl2) ◽  
pp. 4401 ◽  
Author(s):  
Luiz Juliano Valério Geron ◽  
Fabiana Gomes Costa ◽  
Jocilaine Garcia ◽  
Anderson Moura Zanine ◽  
Edmiar Barbosa de Oliveira ◽  
...  

<p>This study aimed to evaluate nutrient intake and digestibility by sheep fed diets containing residue from the extraction of tamarind pulp (RETP). We used four sheep with a mean body weight (BW) of 40.38 kg, whose pens were distributed in a Latin square design. The diet treatments contained 0, 5, 10 and 15% of RETP. The variables were subjected to analysis of variance and the differences observed were tested using regression equations at 5% significance. The intake of dry matter (DM), organic matter (OM), crude protein (CP), total carbohydrates (TC), and non-fiber carbohydrates (NFC),expressed in g day-1, % BW and g kg0,75-1, were not significantly affected (p&gt;0.05) by the inclusion of RETP. The intake of ether extract (EE) and neutral detergent fiber (NDF) showed a linear increase (p&lt;0.05) as RETP content rose. However, the digestibility of DM, CP, TC, and total digestible nutrients (TDN) decreased linearly (p&lt;0.05), while the digestibility of EE, NDF, ADF, and NFC, with average values of 62.12%; 79.95%; 41.59%; 37.12%, and 91.57%, respectively, were not significantly affected (p&gt;0.05) when RETP was included in the diet. The inclusion of up to 15% of the residue from the extraction of tamarind pulp changes the intake of ether extract and neutral detergent fiber, and reduced dry matter, crude protein, and total carbohydrates in the sheep’s diet.</p>


2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 340-341
Author(s):  
Hansol Kim ◽  
Jung Yeol Sung ◽  
Beob G Kim

Abstract The objective was to investigate the influence of crude protein (CP) concentrations in the basal diet on the metabolizable energy (ME) of feed ingredients determined using the difference procedure in pigs. Twelve barrows (73.7 ± 5.5 kg body weight) individually housed in metabolism crates were used. A low-protein basal diet (LPBD, 7.4% CP) was composed of 97.5% corn as the sole energy source and a high-protein basal diet (HPBD, 14.3% CP) was composed of 78.0% corn and 19.6% soybean meal. Four additional diets were prepared by replacing 20% of the energy sources in the basal diets with full-fat soybean (FFSB, 37.7% CP) or soy protein isolate (SPI, 87.6% CP). The 6 experimental diets were fed to 12 pigs employing a replicated 6 × 4 incomplete Latin square design with 4 periods. Each period was consisted of 4 days of adaptation and 4 days of collection period and the marker-to-marker method was used for fecal collection. Urinary gross energy output was greater (243 vs. 176 kcal/d; P &lt; 0.05) in the HPBD group compared with the LPBD group. While ME in the HPBD was greater (3,418 vs. 3,322 kcal/kg; P &lt; 0.05) than in the LPBD, ME in FFSB diets (3,585 and 3,633 kcal/kg in the LPBD and HPBD group, respectively) and SPI diets (3,537 and 3,537 kcal/kg, respectively) were not different between the 2 basal diet groups. Metabolizable energy of the test ingredients was less (P &lt; 0.05) in pigs fed the HPBD group (4,565 and 4,111 kcal/kg in FFSB and SPI, respectively) compared with pigs fed the LPBD group (4,756 and 4,517 kcal/kg in FFSB and SPI, respectively). In conclusion, as the protein in the basal diet increases, metabolizable energy in a test ingredient determined using the difference procedure decreases mainly due to greater urinary energy output in pigs.


2020 ◽  
Vol 4 (3) ◽  
Author(s):  
Diego A Rodriguez ◽  
Su A Lee ◽  
María R C de Godoy ◽  
Hans H Stein

Abstract Two experiments were conducted to determine effects of extrusion on energy and nutrient digestibility in soybean hulls. One source of soybean hulls was ground and divided into two batches. One batch was used without further processing, whereas the other batch was extruded. In Exp. 1, four diets were formulated to determine crude protein (CP) and amino acid (AA) digestibility in soybean hulls. A soybean meal-based diet in which soybean meal provided all the CP and AA was formulated. Two diets were formulated to contain 30% nonextruded or extruded soybean hulls and 18% soybean meal. An N-free diet that was used to determine the endogenous losses of CP and AA was also used. Eight growing barrows (initial body weight = 37.0 ± 3.9 kg) had a T-cannula installed in the distal ileum and were allotted to a replicated 4 × 4 Latin square design. Each experimental period lasted 7 d with the initial 5 d being the adaptation period and ileal digesta were collected for 8 h on day 6 and 7. Results indicated that extrusion of soybean hulls did not change the standardized ileal digestibility (SID) of CP and most AA with the exception that the SID of Ile and Leu tended (P &lt; 0.10) to be greater in extruded than nonextruded soybean hulls. In Exp. 2, three diets were formulated to determine energy digestibility in soybean hulls. One corn-soybean meal based basal diet, and two diets that contained corn, soybean meal, and 32% extruded or nonextruded soybean hulls were formulated. Twenty-four growing barrows (initial body weight = 59.9 ± 3.4 kg) were allotted to a randomized complete block design. Pigs were housed individually in metabolism crates and feces and urine were collected separately for 4 d after 5 d of adaptation. The apparent total tract digestibility (ATTD) of gross energy (GE) and the digestible energy (DE) and metabolizable energy (ME) were reduced (P &lt; 0.05) in diets containing nonextruded or extruded soybean hulls compared with the basal diet. However, the ATTD of GE and values for DE and ME in soybean hulls were not improved by extrusion. Likewise, extrusion did not change the concentration of total dietary fiber in soybean hulls. In conclusion, there were no effects of extrusion of soybean hulls on SID of AA, energy digestibility, or ME concentration in soybean hulls.


2005 ◽  
Vol 85 (3) ◽  
pp. 355-363 ◽  
Author(s):  
C. Kaufmann ◽  
W. C. Sauer ◽  
M. Cervantes ◽  
Y. Zhang ◽  
J. He ◽  
...  

Studies were carried out to determine the digestibilities of amino acids (AA) and energy in five sources of rice bran (RB). The sources of RB included two that were not further processed, a defatted, an extruded, and an atypical source of RB consisting mainly of starchy endosperm referred to as polish. Six barrows, average initial body weight 36.5 kg, were fitted with a simple T-cannula at the distal ileum, and fed six diets according to a 6 × 6 Latin square design. The five RB-containing diets contained 53.8% corn, 19.9% soybean meal, and 23.1% RB. The basal diet contained 71.0% corn and 26.3% soybean meal. The dietary allowance was provided at a rate of 4% (wt/wt) of the individual body weight determined at the initiation of each experimental period. Each experimental period comprised 11 d. Following a 7-d adaptation period, faeces were collected for 48 h and ileal digesta for 24 h in two 12-h periods. Chromic oxide was used as the digestibility marker. There was considerable variation in AA digestibilities among the RB samples. By aid of the difference method under the dietary conditions specified, the apparent ileal digestibilities of lysine, methionine, threonine, and tryptophan ranged from 62.6 to 82.2, 61.4 to 76.0, 58.4 to 82.8, and 58.8 to 82.6%, respectively. The digestibilities were lowest in the defatted source of RB and highest in polish. Not including polish of which the total tract energy digestibility was 88.2%, the digestibilities of the other samples ranged from 60.5 to 65.8%. A small proportion of the variation in AA digestibility can be attributed to the content of crude protein, fat and ash in RB. Key words: Amino acids, digestibility, energy, pigs, rice bran


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