scholarly journals The influence of dietary energy and zinc source and concentration on performance, trace mineral status, and gene expression of beef steers

2020 ◽  
Vol 4 (4) ◽  
Author(s):  
Remy N Carmichael-Wyatt ◽  
Olivia N Genther-Schroeder ◽  
Stephanie L Hansen

Abstract The objective of this study was to determine the effects of increased supplemental Zn from differing sources on growth performance of steers fed diets differing in net energy. Angus steers (n = 72, 324 ± 2.1 kg) with Genemax gain scores of 3, 4, or 5 were blocked by BW and stratified by Genemax gain score into 12 pens of 6 steers each for 158 d. Pens were randomly assigned to 1 of 3 Zn treatments (ZNTRT): 1) control (no supplemental Zn, analyzed 33 mg Zn/kg DM; CON); 2) inorganic Zn (CON + 120 mg supplemental Zn/kg DM as ZnSO4 for entire trial; INZN); or 3) 120 mg supplemental Zn/kg DM as Zn-amino acid complex (Availa-Zn; Zinpro, Eden Prairie, MN) for first 60 d, then a blend of ZnSO4 and Zn-AA complex (CON + 60 mg supplemental Zn/kg DM as ZnSO4 + 60 mg supplemental Zn/kg DM as Zn-amino acid complex) for the remainder of the trial (ZNBLD). Two dietary energy strategies (ENERGY) were formulated to reach ADG rates of 1) 1.6 kg/d (LE) or 2) 2.0 kg/d (HE) utilizing a 3 × 2 factorial arrangement (12 steers/treatment). All steers were fed LE for a 60 d growing period, then pens were randomly assigned to ENERGY treatments fed the remaining 91 d. Day 60 BW tended to be greater (P = 0.07) in steers receiving supplemental Zn vs. CON. Liver Cu was decreased in Zn supplemented steers vs. CON (P = 0.02). Liver Zn concentrations on d 56 did not differ for Zn vs. CON (P = 0.22) nor were there differences due to Zn source (P = 0.98). There were or tended to be ZNTRT × ENERGY effects for d 67–90 ADG and G:F (P ≤ 0.01), and d 122 BW and d 90–122 G:F (P ≤ 0.10) driven by improved performance for ZNBLD-HE over ZNBLD-LE, while ENERGY within CON and INZN did not differ. Day 90–122 ADG, overall ADG and overall G:F was greater (P ≤ 0.02) and d 67–90 G:F tended to be greater (P = 0.10) for HE vs. LE. No ZNTRT × ENERGY or ZNTRT effects were detected for HCW, REA, BF, KPH, MS, or YG (P ≥ 0.37) while HE increased HCW, BF, MS, and YG compared with LE (P ≤ 0.05). In the liver, ZNTRT affected d 97 MT1A expression (P = 0.03) where INZN was greater than ZNBLD or CON (P ≤ 0.02), while ZIP14 was unaffected due to ZNTRT, ENERGY, or the interaction (P ≥ 0.39). Supplying supplemental Zn as ZNBLD during the transition period appeared to improve performance measures, but no final performance advantages were noted due to increased supplemental Zn, regardless of source. Additionally, differences in liver MT1A expression may indicate differing post-absorptive metabolism between Zn sources.

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 212-213
Author(s):  
Chanda Engel ◽  
Gary Tibbetts ◽  
Mark Branine

Abstract A 42-d research study evaluated effects of feeding beef steers increasing levels of dietary Zn from Zn methionine (AAC-Zn; ZINPRO, Zinpro Corporation, Eden Prairie, MN) with or without supplemental Cu from Cu amino acid complex (AAC-Cu; Availa Cu, Zinpro Corp.) concurrently with ractopamine hydrochloride (RAC; Optaflexx, Elanco Animal Health, Greenfield, IN). One hundred-twenty steers (mean BW = 624 kg), ≤ 50 d from projected harvest were randomized to three pens (40 steers/pen) equipped with GrowSafe Systems (Calgary AB, Canada) feed bunk technology. Each pen was assigned to one of three treatment diets: 1) basal finishing diet + 30 mg Zn/kg DM from AAC-Zn (CON); 2) basal finishing diet + 90 mg Zn/kg DM from AAC-Zn (AAC-Zn90); or 3) AAC-Zn90 diet + 10 mg Cu/kg DM from AAC-Cu (AAC-Zn/Cu). Following allocation to treatments, cattle were acclimated to pen cohorts and GrowSafe feed bunks for 7 days. All steers were fed 300 mg RAC∙hd-1∙d-1 starting 35 d prior to harvest. Individual feed intake measurements began with RAC feeding and continued for 35 d until cattle were shipped for harvest. Carcass data were collected from each steer. Data were analyzed with individual animal as the experimental unit using PROC MIXED and PROC GLIMMIX procedures of SAS 9.4 (SAS Institute, Cary, NC). Numerically AAC-Zn90 fed cattle had heavier carcass weights than CON and AAC-Zn/Cu. Steers fed AAC-Zn90 had greater (P = 0.02) marbling scores compared to AAC-Zn/Cu. Steers fed AAC-Zn90 had lower backfat thickness (P = 0.02) and numerically greater marbling scores compared to CON. A biphasic program of feeding a lower level of AAC-Zn for the duration of the finishing phase followed by an increased rate of AAC-Zn during RAC feeding may optimize overall live and carcass response and improve total individual animal value.


2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 51-51
Author(s):  
Remy N Carmichael ◽  
Stephanie L Hansen ◽  
Olivia Genther-Schroeder

Abstract The objective was to determine if steer performance improved with supplemental Zn above recommended concentrations with increasing growth rate. Angus steers (n = 72; 324 ± 2.1kg BW) were blocked by BW within growing diets to one of three Zn strategies (ZNTRT) 1) no supplemental Zn (analyzed 36 mg Zn/kg DM; CON), 2) supranutritional Zn as ZnSO4(CON + 120 mg Zn/kg DM; INZN), or 3) supranutritional Zn blend (CON + 60 mg Zn/kg as ZnSO4 + 60 mg Zn/kg as Zn-amino acid complex; ZNBLD) fed for 60 d, then assigned to dietary energy strategies (ENERGY) targeting growth rates of 1.6 (LOW) or 2 kg/d (HI). On d 60, HI steers were transitioned for 7 d and all animals received respective treatments for an additional 91 d finishing period (n = 12). Data were analyzed as a 3×2 factorial in Proc Mixed of SAS with block as fixed effect; steer was experimental unit. After 60 d of dietary treatment BW tended to be greater in Zn-fed steers vs. CON (P = 0.07). Finishing DMI within HI was similar between INZN and ZNBLD, which were greater than CON, while within LOW no differences were detected due to ZNTRT (ZNTRT×ENERGY; P = 0.01). Neither ZNTRT×ENERGY nor ZNTRT effects (P ≥ 0.37) were detected for finishing ADG, HCW, marbling score, yield grade or backfat. However, finishing period ADG, hot carcass weight, marbling score, yield grade, and back fat were greater in HI vs. LOW (P ≤ 0.05). Plasma NEFA concentrations were lesser for steers receiving HI vs. LOW (P = 0.02), while BUN was unaffected by ZNTRT or ENERGY (P ≥ 0.42). Steer performance was influenced by supranutritional Zn and dietary energy.


2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 157-158
Author(s):  
Jeff Heldt ◽  
Ben Holland ◽  
Alyssa Word ◽  
Kendall Karr

Abstract Crossbred beef steers (n = 2,420; 357 ± 16 kg BW) were used to evaluate the effects of supplemental trace mineral sources on the performance and carcass characteristics on feedlot cattle. Steers were housed in 20 pens (120–125 steers per pen) with 10 pens per treatment. Supplemental trace mineral treatments (100% DM) consisted of: control: 8.3 mg/kg of Cu (100% CuSO4), 83.4 mg/kg of Zn (64.1% ZnSO4/35.9% Zn amino acid complex), and 28.7 mg/kg of Mn (100% MnSO4); hydroxy: 8.3 mg/kg of Cu (100% basic Cu chloride), 82.9 mg/kg of Zn (100% Zn hydroxychloride), and 19.3 mg/kg of Mn (100% Mn hydroxychloride). Steers were fed trace mineral treatments for 158 d and harvested at a commercial abattoir. Data were analyzed as a randomized complete block design with pen as the experimental unit. There were no differences between treatments in DMI, ADG, feed:gain, final BW, or mortality (P ≥ 0.38); however, morbidity tended (P = 0.06) to be greater for hydroxy than control (3.44 or 2.20%, respectively). Hot carcass weight, dressing percentage, marbling score, and longissimus muscle area were unaffected (P ≥ 0.28) by treatment, though backfat tended (P = 0.07) to be greater for hydroxy compared to control (1.55 or 1.51 cm, respectively). Distributions of quality grades were not different (P ≥ 0.14) with the exception of increased (P = 0.01) Prime carcasses in control compared to hydroxy (2.57 or 1.18%, respectively). Distributions of yield grades 2, 3, and 4 were not different (P ≥ 0.17) between treatments, though percentage of carcasses assigned 1 was reduced (P = 0.01) and 5 was increased (P = 0.05) in hydroxy compared to control. These data indicate steers fed 100% hydroxychloride trace minerals perform similiarly to steers fed CuSO4, MnSO4, and a ZnSO4/Zn amino acid complex combination.


2003 ◽  
Vol 23 (1) ◽  
pp. 25-32
Author(s):  
K. M. Downs ◽  
R. A. Norton ◽  
K. S. Macklin ◽  
J. B. Hess

Development ◽  
1965 ◽  
Vol 13 (3) ◽  
pp. 341-356
Author(s):  
F. S. Billett ◽  
Rosalba Collini ◽  
Louie Hamilton

In many bacterial systems chloramphenicol has been shown to inhibit protein synthesis (Hahn & Wisseman, 1951; Gale & Folkes, 1953). The precise mechanism of this inhibition is not clear, although the evidence suggests that the interaction of the soluble RNA-amino acid complex with the ribosomes is prevented because the attachment of the messenger RNA to the ribosomes is itself impaired (Lacks & Gros, 1959; Nathans & Lipman, 1961; Jardetsky & Julian, 1964; Julian & Jardetsky, 1964). In contrast to its effect on bacterial systems, chloramphenicol has been reported to have little or no action on the protein synthesis by cell-free extracts of mammalian cells (Rendi, 1959; Ehrenstein & Lipmann, 1961). A basis for this resistance has been proposed by Vazquez (1964), who finds that whereas bacterial ribosomes bind chloramphenicol, ribosomes from other organisms do not. Nevertheless, it cannot be stated with any confidence that chloramphenicol has no effect on the protein synthesis of animal cells.


2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 82-83
Author(s):  
Pornpim Aparachita ◽  
Scott Carter ◽  
Afton Sawyer ◽  
Jared Harshman ◽  
Zach Rambo ◽  
...  

Abstract Previously, we reported that supplementing a water soluble zinc via drinking water (0 to 80 mg/L) to nursery pigs improved ADG and G:F. To evaluate the efficacy of higher titrated levels of this water soluble zinc amino acid complex (ProPath®Zn LQ, Zinpro Corporation, Eden Prairie, MN) on growth performance, 280 crossbred pigs (5.5 kg BW; 19 d of age) were randomly allotted to four water treatments (7 pens/treatment; 10 pigs/pen). The water treatments were 0, 40, 80 and 160 mg Zn/L of water. Pigs were fed in 4 dietary phases with complex, nutrient-dense, corn-soybean meal-based diets: Phase 1 and 2 (2,500 and 1,750 mg Zn as ZnO/kg; d 1–7 and 7–14, respectively) and Phase 3 and 4 (200 mg Cu as CuSO4/kg; d 14–23 and 23–42, respectively). Pigs and feeders were weighed weekly to determine ADG, ADFI, and G:F. Water meters were used to record and calculate water disappearance and zinc intake. Data were analyzed as a randomized complete block design. Orthogonal polynomial contrasts were used to determine linear and curvilinear effects. Water and total zinc intake increased linearly (P < 0.001) with increasing water zinc concentration. From d 0–14 when high dietary zinc was fed, there were no differences (P > 0.10) in ADG, ADFI, or G:F. However, from d 14–42 when basal levels of zinc were fed, quadratic improvements in ADG (0.545, 0.561, 0.578, 0.546 kg; P < 0.05) and G:F (0.686, 0.706, 0.723, 0.702; P < 0.01) were observed with increasing zinc via water. Similarly for d 0–42, ADG (0.435, 0.440, 0.454; 0.434 kg; P = 0.07), G:F (0.726, 0.740, 0.763, 0.749; P = 0.05) and average ending wt (23.73, 23.97, 24.55, 23.70 kg; P = 0.07) improved quadratically with increasing zinc. In conclusion, supplementing ProPath®Zn LQ via water resulted in improvements in ADG and G:F for nursery pigs.


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