scholarly journals Reduced concentrations of limestone and monocalcium phosphate in diets without or with microbial phytase did not influence gastric pH, fecal score, or growth performance, but reduced bone ash and serum albumin in weanling pigs

2021 ◽  
Author(s):  
L Vanessa Lagos ◽  
Su A Lee ◽  
Mike R Bedford ◽  
Hans H Stein
Keyword(s):  
Growth Performance ◽  
Phase 1 ◽  
Average Daily Gain ◽  
Gastric Ph ◽  
Weanling Pigs ◽  
Monocalcium Phosphate ◽  
Bone Ash ◽  
Microbial Phytase ◽  
Stomach Ph ◽  
Fecal Score

Abstract An experiment was conducted to test the hypothesis that reducing limestone and monocalcium phosphate in diets for weanling pigs by lowering the concentration of Ca and P or by including microbial phytase in the diet will reduce stomach pH and fecal score and will improve growth performance of pigs. A total of 160 weanling pigs (5.75 ± 1.04 kg) were allotted to 4 corn-soybean meal-based diets in a completely randomized design with 5 pigs per pen. Diets for phase 1 (d 1 to 15) were formulated using a 2 × 2 factorial design with 2 concentrations of Ca and P (adequate or deficient levels of total Ca and digestible P) and 2 inclusion levels of phytase (0 or 2,000 units/kg feed). Phytase was assumed to release 0.16% total Ca and 0.11% digestible P. Common diets were fed in phases 2 (d 16 to 21) and 3 (d 22 to 35). Fecal scores were recorded in phase 1 and on d 15, gastric pH was measured and a blood sample and the right femur were collected from 1 pig per pen. Growth performance data were recorded within each phase. Results indicated that in phase 1, at deficient dietary Ca and P, pigs fed the diet with phytase had greater (P < 0.05) average daily gain (ADG) and gain to feed (G:F) compared with pigs fed the diet without phytase, but in diets with adequate levels of Ca and P, no effect of phytase inclusion was observed (interaction, P < 0.05). Without phytase, pigs fed the diet with deficient Ca and P had reduced (P < 0.05) G:F compared with pigs fed the diet with adequate Ca and P, but if phytase was included, there was no effect of Ca and P on G:F (interaction, P < 0.05). For phases 2 and 3, and from d 1 to 35, no differences among dietary treatments were observed for ADG or G:F. Bone ash was greater (P < 0.05) in pigs fed diets with adequate Ca and P than in pigs fed diets with deficient Ca and P, but no effect of phytase inclusion was observed on bone ash. Concentrations of Ca and P did not affect stomach pH or fecal score, but pigs fed diets with phytase tended (P < 0.10) to have reduced stomach pH and fecal score compared with pigs fed diets without phytase. Pigs fed diets with adequate Ca and P had greater (P < 0.05) albumin in serum than pigs fed the Ca- and P-deficient diets. In conclusion, phytase inclusion in phase 1 diets may reduce diarrhea, but lowering Ca and P does not reduce stomach pH or fecal score and decreases bone ash, although growth performance during the entire weanling period is not affected.

61 Effects of Reducing the Concentration of Ca and P and Increasing Microbial Phytase on Gastric Ph, Fecal Score, Growth Performance, and Bone Ash of Weanling Pigs

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 44-45
Author(s):  
Vanessa Lagos ◽  
Mike R Bedford ◽  
Hans H Stein
Keyword(s):  
Growth Performance ◽  
Block Design ◽  
Control Diet ◽  
Phase 1 ◽  
Average Daily Gain ◽  
Gastric Ph ◽  
Weanling Pigs ◽  
Bone Ash ◽  
Microbial Phytase ◽  
Fecal Score

Abstract An experiment was conducted to test the hypothesis that lowering dietary Ca and P reduces gastric pH and diarrhea of weanling pigs, but microbial phytase overcomes negative effects of low Ca and P on growth performance and bone ash. A total of 320 weanling pigs (6.35 ± 0.87 kg) were allotted to 8 corn-soybean meal-based diets in a randomized complete block design with 5 pigs/pen. Two phase 1 (d 1 to 14) control diets contained 100 or 50% of total Ca and digestible P relative to the requirement, and 6 diets in which 500, 2,000, or 16,000 units/kg of phytase was added to each control diet were formulated. Common diets were fed in phases 2 (d 15 to 27) and 3 (d 28 to 42). Fecal scores were recorded in phase 1 and growth performance data were recorded within each phase. Gastric pH was measured in 1 pig/pen on d 14; on d 14 and 42, the right femur of 1 pig/pen was collected. Data were analyzed using contrast statements in SAS. Results indicated that during phase 1, lowering Ca and P did not reduce gastric pH or fecal score, but the 50%-diets reduced (P < 0.05) average daily gain and average daily feed intake of pigs compared with the 100%-diets (Table 1). Phytase above 500 units/kg increased (P < 0.05) gain:feed ratio and tended (P < 0.10) to decrease gastric pH. Pigs fed the 50%-diets had reduced (P < 0.05) bone ash at d 14 and 42 compared with pigs fed the 100%-diets regardless of phytase inclusion level. In conclusion, reducing Ca and P in diets for weanling pigs does not decrease gastric pH or fecal score, but compromises growth performance and bone mineralization. However, super-dosing of phytase increases G:F of pigs regardless of dietary Ca and P concentration.

Increased microbial phytase increased phytate destruction, plasma inositol, and feed efficiency of weanling pigs, but reduced dietary calcium and phosphorus did not affect gastric pH or fecal score and reduced growth performance and bone ash

2021 ◽  
Author(s):  
L Vanessa Lagos ◽  
Mike R Bedford ◽  
Hans H Stein
Keyword(s):  
Growth Performance ◽  
Inositol Phosphate ◽  
Block Design ◽  
Control Diet ◽  
Phase 1 ◽  
Average Daily Gain ◽  
Gastric Ph ◽  
Weanling Pigs ◽  
Bone Ash ◽  
Fecal Score

Abstract An experiment was conducted to test 2 hypotheses: 1) reducing dietary Ca and P reduces gastric pH and diarrhea in weanling pigs; 2) negative effects of low Ca and P on pig growth performance may be overcome if phytase is added to the diets. A total of 320 weanling pigs (6.35 ± 0.87 kg) were allotted to 8 corn-soybean meal-based diets in a randomized complete block design with 5 pigs per pen. Two phase 1 (d 1 to 14) control diets containing 100 or 50% of total Ca and digestible P relative to the requirement, and 6 diets in which 500, 2,000, or 16,000 units of phytase/kg feed (FTU) were added to each control diet were formulated. Phytase was assumed to release 0.16% total Ca and 0.11% digestible P. Common diets were fed in phases 2 (d 15 to 27) and 3 (d 28 to 42). Growth performance data were recorded within each phase. Data for fecal scores and gastrointestinal pH were recorded for phase 1. Colon content (d 14), the right femur (d 14 and 42), and blood samples (d -1, 14, 27, and 42) were collected from 1 pig per pen. In phase 1, reducing Ca and P did not reduce gastric pH or fecal score, but pigs fed the 50% diets had reduced (P < 0.05) average daily gain (ADG) and average daily feed intake (ADFI) compared with pigs fed the 100% diets. In both 50 and 100% diets, phytase above 500 FTU increased (P < 0.05) gain:feed ratio (G:F) and tended (P < 0.10) to reduce gastric pH of pigs. From d 1 to 42, pigs fed the 50% diets tended (P < 0.10) to have reduced ADG and ADFI compared with pigs fed the 100% diets, but among the 100% diets, pigs tended (P < 0.10) to have a linear increase in G:F as phytase level increased. Pigs fed the 50% diets had reduced (P < 0.05) concentrations of inositol phosphate esters (IP) in the colon and reduced bone ash (d 14 and 42) compared with pigs fed the 100% diets. Phytase did not affect bone ash or most blood metabolites. Concentrations of IP in the colon decreased, whereas plasma inositol increased (d 14; P < 0.05) in pigs fed diets with phytase (≥ 500 FTU). In pigs fed the 100% diets, IP in the colon linearly decreased (P < 0.05), but plasma inositol linearly increased (P < 0.05) with increasing levels of phytase. In conclusion, reducing Ca and P in diets for weanling pigs did not influence gastric pH or fecal score, but compromised growth performance and bone ash. However, regardless of dietary Ca and P, high doses of phytase increased phytate degradation and inositol absorption, which consequently increased G:F of pigs.

Effect of dietary fermented oats in different nutrient-density diets on growth performance, nutrient digestibility and ileal microorganisms of weanling pigs

2019 ◽  
Vol 59 (12) ◽  
pp. 2230
Author(s):  
J. W. Park ◽  
H. L. Li ◽  
J. K. Kim ◽  
D. H. Nguyen ◽  
I. H. Kim
Keyword(s):  
Growth Performance ◽  
Phase 1 ◽  
Average Daily Gain ◽  
Experimental Period ◽  
Nutrient Digestibility ◽  
Phase 2 ◽  
Weanling Pigs ◽  
Beneficial Effects ◽  
Nutrient Density ◽  
Dietary Treatments

A total of 160 crossbred weanling pigs [(Landrace × Yorkshire) × Duroc, 28 days old] with an initial bodyweight of 8.01 ± 0.74 kg were used in a 42-day trial (Phase 1: Days 1–14, Phase 2: Days 14–42). The experiment was conducted to evaluate the effects of the inclusion of fermented oats (FO) to a corn and soybean meal-based diet on the performance of weanling pigs fed with high nutrient-density (HD) and low nutrient-density (LD) diets. Pigs were randomly distributed into one of four dietary treatments in a 2 × 2 factorial arrangement. Two levels of nutrient density [LD = 3323 (Phase 1: 1–14 days) and 3269 kcal/kg (Phase 2: 14–42 days); HD = 3446 (Phase 1: 1–14 days) and 3325 kcal/kg (Phase 2: 14–42 days), the differences in dietary energy were based on differences in the content of fish meal, animal fat, corn and FO [0%, 5% (Phase 1) or 2.5% (Phase 2)] were used according to the bodyweight and sex of pigs [8 replicate pens per treatment with 5 pigs in a pen (3 gilts and 2 barrows)]. During the overall trial period (Days 1–42), the average daily gain and gain:feed ratio of pigs fed HD and FO diets were significantly higher (P ≤ 0.05) than those of pigs fed with LD and non-fermented oats diets respectively. On Day 14, the apparent total tract digestibility of dry matter and nitrogen of the HD groups were significantly greater (P ≤ 0.05) than those of the LD groups. The apparent total tract digestibility of energy of the FO groups was significantly greater (P ≤ 0.05) than that of the non-fermented oats diet groups during the whole experimental period. Pigs fed with FO showed higher (P ≤ 0.05) ileal Lactobacillus and lower (P ≤ 0.05) Escherichia coli counts compared with those fed with the non-fermented oats diet. In conclusion, administration of FO to a LD diet demonstrated beneficial effects on the growth performance, nutrient digestibility and ileal microorganisms balance of weanling pigs.

scholarly journals PSV-15 Effects of isoquinoline alkaloids on growth performance and blood characteristics of weanling pigs fed corn-soybean meal diets

2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 199-200
Author(s):  
Carly Rundle ◽  
Valeria Artuso-Ponte ◽  
Hans H Stein
Keyword(s):  
Growth Performance ◽  
Soybean Meal ◽  
Total Protein ◽  
Phase 1 ◽  
Average Daily Gain ◽  
Experimental Period ◽  
Isoquinoline Alkaloids ◽  
Weanling Pigs ◽  
Plasma Urea ◽  
Blood Characteristics

Abstract An experiment was conducted to determine effects of isoquinoline alkaloids (IQ) on growth performance and blood characteristics of weanling pigs fed corn-soybean meal-based diets. A total of 160 pigs (6.33 ± 0.61 kg) were allotted to 4 corn-soybean meal-based treatments, with 4 pigs per pen and 10 replicate pens per treatment. A 3-phase feeding program was used with d 0 to 8 as phase 1, d 8 to 21 as phase 2, and d 21 to 34 as phase 3. Within each phase, the 4 diets were identical except for the inclusion of IQ in the feed: 0, 90, 180, or 360 mg IQ/kg complete feed. Pig weights and the feed left in the feeders were recorded on the last d of each phase to calculate average daily gain and average gain:feed ratio. A blood sample was collected from 1 pig per pen on d 8, 21, and 34. Data were analyzed by linear and quadratic contrasts using the Proc MIXED of SAS with coefficients for unequally spaced treatments being generated from Proc IML. Results indicated that there were no differences among treatments in growth performance over the entire experimental period (Table 1). A quadratic increase (P < 0.05) of total protein in plasma was observed in phase 1, with the highest value in plasma of pigs fed the 180 mg/kg IQ diet, and there was a tendency for plasma urea N to increase in phases 2 and 3 (linear; P = 0.07) if IQ was added to the diet. In conclusion, although IQ supplementation did not effect overall growth performance, results indicate that absorption of amino acids was likely improved by inclusion of IQ in the diets as evidenced by the increased plasma urea nitrogen and increased total protein in plasma.

scholarly journals Formulating diets based on digestible calcium instead of total calcium does not affect growth performance or carcass characteristics, but microbial phytase ameliorates bone resorption caused by low calcium in diets fed to pigs from 11 to 130 kg

2021 ◽  
Author(s):  
L Vanessa Lagos ◽  
Su A Lee ◽  
Mike R Bedford ◽  
Hans H Stein
Keyword(s):  
Bone Resorption ◽  
Growth Performance ◽  
Blood Sample ◽  
Phase 1 ◽  
Carcass Characteristics ◽  
Phase 2 ◽  
Diet Formulation ◽  
Bone Ash ◽  
Microbial Phytase ◽  
The Right

Abstract An experiment was conducted to test the hypothesis that the requirement for Ca expressed as a ratio between standardized total tract digestible (STTD) Ca and STTD P obtained in short-term experiments may be applied to pigs fed diets without or with microbial phytase from 11 to 130 kg. In a 5-phase program, 160 pigs (body weight: 11.2 ± 1.8 kg) were randomly allotted to 32 pens and 4 corn-soybean meal based diets in a 2 × 2 factorial design with 2 diet formulation principles (total Ca or STTD Ca), and 2 phytase inclusion levels (0 or 500 units/kg of feed) assuming phytase released 0.11% STTD P and 0.16% total Ca. The STTD Ca:STTD P ratios were 1.40:1, 1.35:1, 1.25:1, 1.18:1, and 1.10:1 for phases 1 to 5, and STTD P was at the requirement. Weights of pigs and feed left in feeders were recorded at the end of each phase. At the conclusion of phase 1 (d 24), 1 pig per pen was euthanized and a blood sample and the right femur were collected. At the end of phases 2 to 5, a blood sample was collected from the same pig in each pen. At the conclusion of the experiment (d 126), the right femur of 1 pig per pen was collected and carcass characteristics from this pig were measured. No interactions were observed between diet formulation principle and phytase inclusion for growth performance in any phase and no differences among treatments were observed for overall growth performance. Plasma Ca and P and bone ash at the end of phase 1 were also not influenced by dietary treatments. However, on d 126, pigs fed non-phytase diets formulated based on total Ca had greater bone ash than pigs fed STTD Ca based diets, but if phytase was used, no differences were observed between the 2 formulation principles (interaction P &lt; 0.05). At the end of phases 2 and 3, pigs fed diets without phytase had greater (P &lt; 0.05) plasma P than pigs fed diets with phytase, but no differences were observed at the end of phases 4 and 5. A negative quadratic effect (P &lt; 0.05) of phase (2 to 5) on the concentration of plasma Ca was observed, whereas plasma P increased (quadratic; P &lt; 0.05) from phase 2 to 5. However, there was no interaction or effect of diet formulation principle or phytase inclusion on any carcass characteristics measured. In conclusion, STTD Ca to STTD P ratios can be used in diet formulation for growing-finishing pigs without affecting growth performance or carcass characteristics and phytase inclusion ameliorates bone resorption caused by low dietary Ca and P.

PSIII-16 Growth Performance of Weaned Pigs Fed Barley Differing in Fermentable Starch and Fiber Profile

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 175-176
Author(s):  
Joaquin J Sanchez Zannatta ◽  
L F Wang ◽  
Eduardo Beltranena ◽  
Aaron D Beattie ◽  
Rex N Newkirk ◽  
...  
Keyword(s):  
Energy Source ◽  
Growth Performance ◽  
Steam Explosion ◽  
Phase 1 ◽  
Average Daily Gain ◽  
Barley Grain ◽  
Gut Health ◽  
Weaned Pigs ◽  
Cereal Grain ◽  
Hulled Barley

Abstract Barley grain containing more fermentable starch or fiber might be an attractive energy source in weaned pig diets due to benefits on gut health. Barley rapidly-fermentable carbohydrates may serve as prebiotic and slowly-fermentable fiber may decrease diarrhea in weaned pigs. Steam-explosion processing may disrupt the fiber matrix of hulls, increasing slowly-fermentable fiber of barley. To explore, 220 pigs were fed 1 of 5 diets containing 60% cereal grain: 1) low-fermentable hulled barley (LFB); 2) LFB steam-exploded (LFB-E; 1.2 MPa, 120 s); 3) high β-glucan (10% DM) hull-less barley (HFB); 4) high amylose (17% DM) hull-less barley (HFA); or 5) low-fermentable wheat (LFW). Diets were fed starting 1-week post-weaning and formulated to provide 2.4 and 2.3 Mcal net energy (NE)/kg, 5.5 and 5.1 g standardized ileal digestible lysine/Mcal NE for phase 1 (day 1–14) and phase 2 (day 15–35), respectively. For the entire trial (day 1–35), average daily feed intake (ADFI) and average daily gain (ADG) of pigs did not differ among diets. Gain:feed (G:F) did not differ between LFB and LFW diets, but steam-explosion of hulled barley reduced (P &lt; 0.05) G:F. Feces consistency did not differ between LFB and LFW diets, but was better (P &lt; 0.05) for LFB than HFB, HFA and LFB-E diets. For phase 1, G:F of pigs was lower (P &lt; 0.05) for LFB-E diet than LFW diet. For days 22–28, LFB-E diet had greater (P &lt; 0.01) ADFI than HFA diet and tended (P = 0.09) to have a greater ADG than HFB diet. In conclusion, hulled or hull-less barley grain replaced wheat grain without affecting growth performance in weaned pigs. Hulled barley increased feces consistency. Steam-explosion of hulled barley did not increase growth performance of weaned pigs. Barley grain is an attractive energy source for weaned pigs for managing growth and feces consistency.

scholarly journals PSVI-1 Dietary protease supplementation on growth performance and post-weaning diarrhea of nursery pigs

2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 191-192
Author(s):  
Jorge Y Perez-Palencia ◽  
David A Clizer ◽  
Christian Ramirez-Camba ◽  
Ryan S Samuel ◽  
Crystal L Levesque
Keyword(s):  
Growth Performance ◽  
Phase 1 ◽  
Dietary Treatment ◽  
Experimental Period ◽  
Phase 2 ◽  
Deficient Diet ◽  
Nursery Pigs ◽  
Feeding Program ◽  
Dietary Treatments ◽  
Fecal Score

Abstract This experiment evaluated the effect of dietary protease supplementation on pig’s growth performance and post-weaning diarrhea in a 43-d trial. A total of 936 weaned pigs [21 d of age, initial BW 5.87 ± 0.31 kg] were used in a 2 × 2 factorial design (2 nutritional levels × 2 protease levels). The nutritional levels corresponded to a standard feeding program and a reduced feeding program (15% lower SID Lys) and without or with protease supplementation (0.0125% inclusion). Piglets were distributed in 36 pens with 26 pigs per pen and 9 replicates per dietary treatment. Pigs received a common Phase 1 diet and experimental diets in Phase 2, 3, and 4, (0.91, 3.4, 4.5, and 24.0 kg/pig, respectively). Phytase was included at 2,500 U in Phase 2 – 4, all diets met energy and AA:Lys requirements for nursery pigs. Feed intake and body weight were determined every 2 weeks. A pen fecal score assessment was performed at d7, 10, and 14. Performance data were analyzed using the PROC MIXED procedure and fecal score data using the PROC FREQ procedure (SAS Inst., Inc., Cary, NC). No significant interactions (P &gt; 0.05) were observed between dietary treatments. From d15 to 43 and during overall experimental period, pigs fed standard diets had greater BW, ADG, and G:F (P &lt; 0.05) than pigs fed deficient diets (Table 1). On d7, pigs fed deficient diet had more (X2 &lt; 0.05) soft and watery feces, and consequently less normal feces compared to pigs fed standard diets (Figure 1). On d7, 10, and 14, pigs fed diets with protease supplementation had more (X2 &lt; 0.05) normal feces compared to control pigs. Overall, effects of dietary protease supplementation on growth performance were not expressive under the conditions of this study but protease addition benefited intestinal health of nursery pigs.

Effects of dietary Korean garlic extract aged by Leuconostoc mesenteroides KCCM35046 on growth performance, digestibility, blood profiles, gas emissions, and microbiota in weanling pigs

2020 ◽  
Vol 100 (3) ◽  
pp. 462-469
Author(s):  
J.H. Cho ◽  
S.D. Liu ◽  
I.H. Kim
Keyword(s):  
Growth Performance ◽  
Average Daily Gain ◽  
Dietary Supplementation ◽  
Experimental Unit ◽  
Feed Conversion ◽  
Weanling Pigs ◽  
Gas Emissions ◽  
E Coli ◽  
Gross Energy ◽  
Dietary Treatments

A total of 150 crossbred pigs [(Duroc × Yorkshire) × Landrace; body weight (BW) = 5.74 ± 1.15 kg; weaned at day 21 after birth] were assigned to one of five dietary treatments (six pens per treatment and five pigs per pen) by BW and sex (two barrows and three gilts) for a 6 wk experiment. The pen was considered the experimental unit in the statistical model. Treatments were corn–soybean meal diets supplemented with 0.0033% tiamulin as well as 0%, 0.05%, 0.10%, or 0.20% fermented garlic (FG). The average daily gain, average daily feed intake (ADFI), and feed conversion ratio were linearly (p < 0.05) increased by FG inclusion during days 21–42 and days 0–42. Dietary supplementation of FG linearly increased (p < 0.05) the coefficient of apparent total tract digestibility of dry matter, nitrogen, and gross energy as well as blood immunoglobulin G concentration during week 6. The fecal ammonia (NH3), total mercaptan emissions, and Escherichia coli counts were linearly (p < 0.05) reduced by FG in the diet. Dietary supplementation of FG increased (p < 0.05) ADFI during days 21–42 and days 0–42 and reduced (p < 0.05) fecal NH3 emissions compared with pigs fed tiamulin. In conclusion, the results indicate that FG can enhance growth performance and digestibility, and reduce fecal E. coli counts as well as fecal gas emissions in weanling pigs.

scholarly journals Growth performance and nutrient digestibility of growing and finishing pigs fed multienzyme-supplemented low-energy and -amino acid diets

2020 ◽  
Vol 4 (2) ◽  
pp. 602-615
Author(s):  
Kevin Jerez-Bogota ◽  
Cristian Sánchez ◽  
Jimena Ibagon ◽  
Maamer Jlali ◽  
Pierre Cozannet ◽  
...  
Keyword(s):  
Growth Performance ◽  
Bone Mineralization ◽  
Control Diet ◽  
Phase 1 ◽  
Negative Control ◽  
Nutrient Digestibility ◽  
Ash Content ◽  
Growing Pigs ◽  
Bone Ash ◽  
Diet Type

Abstract A study was conducted to determine the effects of supplementing corn–soybean meal-based diets with a multienzyme on growth performance, bone mineralization, apparent ileal digestibility (AID) and apparent total tract digestibility (ATTD) of nutrients of growing pigs. A total of 276 pigs (body weight [BW] = 33.99 ± 4.3 kg) were housed by sex in 45 pens of 6 or 7 pigs and fed 5 diets (9 pens/diet) in a randomized complete block design. Diets were positive control (PC); and negative control 1 (NC1) or negative control 2 (NC2) without or with multienzyme. The multienzyme used supplied at least 1,800, 1,244, 6,600, and 1,000 units of xylanase, β-glucanase, arabinofuranosidase, and phytase per kilogram of diet, respectively. The PC diet was adequate in all nutrients according to NRC recommendations and had greater digestible P content than NC1 or NC2 diet by 0.134 percentage points. The PC diet had greater net energy (NE) and standardized ileal digestible amino acids (AA) content than NC1 diet by 3%, and than NC2 diet by 5%. The diets were fed in 4 phases based on BW: Phase 1: 34–50 kg; Phase 2: 50–75 kg; Phase 3: 75–100 kg; and Phase 4: 100–120 kg. Nutrient digestibility and bone mineralization were determined at the end of Phase 1. Overall (34–120 kg BW), pigs fed the PC and NC1 diets did not differ in average daily gain (ADG) and average daily feed intake. Pigs fed NC2 diet had lower (P &lt; 0.05) ADG and gain-to-feed ratio (G:F) than those fed PC diet. Pigs fed PC diet had greater (P &lt; 0.05) bone ash content and ATTD of P than those fed NC1 diet. The ATTD of GE for PC diet was greater (P &lt; 0.05) than that for NC2 diet, and tended to be greater (P &lt; 0.10) than that for NC1 diet. Multienzyme interacted (P &lt; 0.05) with negative control diet type on overall ADG and AID of GE such that multienzyme did not affect overall ADG and AID of GE for the NC1 diet, but increased (P &lt; 0.05) overall ADG and AID of GE for NC2 diet by 5.09 and 8.74%, respectively. Multienzyme did not interact with negative control diet type on overall G:F, bone ash content, AID of AA, and ATTD of nutrients. Multienzyme increased (P &lt; 0.05) overall G:F, AID of methionine, ATTD of GE and P, and tended to increase (P = 0.056) bone ash content. The ADG, bone ash content, and ATTD of GE and P for the multienzyme-supplemented diets were similar to (P &gt; 0.10) PC diet. Thus, NE and digestible AA and P can be lowered by ≤5% in multienzyme-supplemented diets without effects on growth performance and bone ash of pigs.

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