scholarly journals A Dual-Targeted Soybean Protein Is Involved in Bradyrhizobium japonicum Infection of Soybean Root Hair and Cortical Cells

2011 ◽  
Vol 24 (9) ◽  
pp. 1051-1060 ◽  
Author(s):  
Marc Libault ◽  
Manjula Govindarajulu ◽  
R. Howard Berg ◽  
Yee Tsuey Ong ◽  
Kari Puricelli ◽  
...  
Keyword(s):  
Bradyrhizobium Japonicum ◽  
Soybean Protein ◽  
Carbon Utilization ◽  
Plant Host ◽  
Cortical Cells ◽  
Symbiotic Interaction ◽  
Carbon Supply ◽  
Glycine Max L ◽  
Soybean Gene

The symbiotic interaction between legumes and soil bacteria (e.g., soybean [Glycine max L.] and Bradyrhizobium japonicum]) leads to the development of a new root organ, the nodule, where bacteria differentiate into bacteroids that fix atmospheric nitrogen for assimilation by the plant host. In exchange, the host plant provides a steady carbon supply to the bacteroids. This carbon can be stored within the bacteroids in the form of poly-3-hydroxybutyrate granules. The formation of this symbiosis requires communication between both partners to regulate the balance between nitrogen fixation and carbon utilization. In the present study, we describe the soybean gene GmNMNa that is specifically expressed during the infection of soybean cells by B. japonicum. GmNMNa encodes a protein of unknown function. The GmNMNa protein was localized to the nucleolus and also to the mitochondria. Silencing of GmNMNa expression resulted in reduced nodulation, a reduction in the number of bacteroids per infected cell in the nodule, and a clear reduction in the accumulation of poly-3-hydroxybutyrate in the bacteroids. Our results highlight the role of the soybean GmNMNa gene in regulating symbiotic bacterial infection, potentially through the regulation of the accumulation of carbon reserves.

scholarly journals LEGUME LECTINS ACTIVATE SYMBIOTIC PROPERTIES OF SPECIFIC RHIZOBIA UNDER SYMBIOSIS FORMATION AND FUNCTIONING

2009 ◽  
Vol 9 ◽  
pp. 43-58
Author(s):  
O.V. Kyrychenko
Keyword(s):  
Bradyrhizobium Japonicum ◽  
Rhizobium Leguminosarum ◽  
Root Nodules ◽  
Nodule Bacteria ◽  
Plant Host ◽  
Pisum Sativum L ◽  
Glycine Max L ◽  
Growth Formation ◽  
Symbiotic Properties ◽  
Legume Lectins

The effect of pea and soybean lectins on the symbiotic properties of nodule bacteria Rhizobium leguminosarum bv. viciae 240б and Bradyrhizobium japonicum 634б during the formation and functioning of symbiosis with pea (Pisum sativum L.) and soybean (Glycine max L. Merr.) plants respectively was investigated. It was shown that incubation of homologous rhizobia with plant-host lectin had increased bacteria nodulation ability at seeds inoculation, nitrogen fixing activity of root nodules and symbiosis efficiency that had resulted in intensification of plants’ photosynthesis, positive changes of legumenous plants growth, formation of vegetative mass and harvest. The use of complex of complex compositions based on the legume lectins and homologous rhizobia for pre-sowing seeds treatment was shown to be perspective in order to increase seed productivity of legumenous plants.

Metabolic Adjustment of Glycine max (L.) Merril in the Presence of Nitrate and Bradyrhizobium japonicum

Agronomy ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1518
Author(s):  
Alberto Mongolo Júnior ◽  
Felipe Girotto Campos ◽  
Gustavo Ribeiro Barzotto ◽  
Jonas Akenaton Venturineli Pagassini ◽  
Maria Aparecida Ribeiro Vieira ◽  
...  
Keyword(s):  
Bradyrhizobium Japonicum ◽  
Dry Mass ◽  
Antioxidant Systems ◽  
Oxygen Species ◽  
Membrane Stabilization ◽  
Total Sugars ◽  
Vegetative Phase ◽  
Glycine Max L ◽  
Metabolic Adjustment ◽  
Plant Dry Mass

Reactive oxygen species are generated during the processes of photosynthesis and nitrate reduction, which can compromise the integrity of biomolecules and membranes. During the vegetative phase of Fabaceae species, around half of translocated carbohydrate is used for nodule growth, while the other half returns to the aerial part with nitrogen incorporated. These sugars may be yet involved with membrane stabilization, signaling, and activation of important genetic pathways for plant development. Thus, the aim was to study the adjustments of the photosynthetic and antioxidant systems and the accumulation of carbohydrates and biomass in Glycine–Bradyrhizobium cultivated with nitrate (NO3−). Four treatments were evaluated in completely randomized blocks. Glycine–Bradyrhizobium was grown with 1.7 mM of NO3− (GB: 1.7 mM NO3−) and without NO3− (GB: 0 mM NO3−), and Glycine was grown with 1.7 mM of NO3− (G: 1.7 mM NO3−) and without NO3− (G: 0 mM NO3−). Glycine–Bradyrhizobium symbiosis contributes to photosynthetic metabolism and total sugars, reduces the action of antioxidant enzymes, and minimizes the use of nitrate in soybean cultivation.; Glycine–Bradyrhizobium with nitrate provided greater plant dry mass in the vegetative phase, along with increased enzymatic activity and reduced nodule mass.

scholarly journals Response of Soybean (Glycine max (L.) Merrill) to Mineral Nitrogen Fertilization and Bradyrhizobium japonicum Seed Inoculation

Agronomy ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1300
Author(s):  
Janusz Prusiński ◽  
Anna Baturo-Cieśniewska ◽  
Magdalena Borowska
Keyword(s):  
Nitrogen Fertilization ◽  
Bradyrhizobium Japonicum ◽  
Plant Density ◽  
Higher Plants ◽  
High Sensitivity ◽  
N Fertilization ◽  
Mineral N ◽  
Plant Nitrogen ◽  
Seed Inoculation ◽  
Glycine Max L

A growing interest in soybean cultivation in Poland has been observed in the recent years, however it faces a lot of difficulties resulting from a poorly understood effectiveness of plant nitrogen fertilization and from the introduction of Bradyrhizobium japonicum to the environment. The aim of the study was to evaluate the consistency of response of two soybean cultivars to three different rates of mineral N fertilization and two seed inoculation treatments with B. japonicum in field conditions over four years regardless of previous B. japonicum presence in the soil. A highly-diversified-over-years rainfall and temperature in the growing season do not allow for a definite statement of the differences resulting from seed inoculation and mineral N fertilization applied separately or jointly in soybean. A high sensitivity of the nodulation process to rainfall deficits was noted, which resulted in a decreased amount of B. japonicum DNA measured in qPCR and dry matter of nodules. ‘Annushka’ demonstrated a higher yield of seeds and protein, higher plants and the 1st pod setting. ‘Aldana’, due to a significant decrease in plant density, produced a higher number of pods, seeds per pod and the 1000 seed weight per plant. Both cultivars responded with an increase in the seed yield after seed inoculation with HiStick, also with an application of 30 and 60 kg N, as well as with Nitragina with 60 kg N.

Role of ethylene in effective establishment of the peanut–bradyrhizobia symbiotic interaction

Plant Biology ◽  
2021 ◽  
Author(s):  
V. L. Muñoz ◽  
M. S. Figueredo ◽  
H. Reinoso ◽  
A. Fabra
Keyword(s):  
Symbiotic Interaction

Parameters Affecting Milling Qualities of Undefatted Soybeans (Glycine max, L. Merill) (2): Selected Physical Properties

2005 ◽  
Vol 1 (5) ◽  
Author(s):  
Abdul Ganiy O. Raji ◽  
John Alaba Victor Famurewa
Keyword(s):  
Moisture Content ◽  
Protein Content ◽  
Soybean Protein ◽  
Soy Flour ◽  
International Institute ◽  
Attrition Mill ◽  
Protein Retention ◽  
Significant Difference ◽  
Glycine Max L ◽  
Flour Yield

Soybean protein is highly rich in the essential amino acid needed by human body. In the developing countries, its use will be more generally acceptable when converted to flour like wheat. This work focused on establishing the optimum conditions of some physical characteristics of soybean for high quality and acceptable soy flour. The characteristics studied for soybean samples subjected to heat treatment of boiling and oven drying were the effect moisture content (MC) and the hull thickness on the quality of the flours. Ten samples of Tax 1485 at different moisture levels ranging from 20.6% to 8.6% and five varieties (Tax 1440-1E, Tax 1740, Tax 1485, Tax 1456 and Tax 1448-2E) obtained from International Institute for Tropical Agriculture (IITA), having different hull thicknesses were used. Soybean with mixed varieties commonly found in the open markets was also used to compare the effects of MC on the studied parameters. Flours obtained from the samples milled in an attrition mill were subjected to proximate, sieve and organoleptic evaluations. The hull thickness was found to have no correlation with protein retention, acceptability and flour yield, but moisture content has a very significant effect on the qualities. Between moisture content of 20.6% and 8.6%, protein and acceptability reduced from 40.0% to 27.7% and 4.3 to 2.2 respectively while yield increased from 0.9% to 5.8%. Though protein was reducing with reduced MC, there was no significant difference (p<0.05) in protein retention between MC of 20.6% and 10.1% but it became significant at 9.4%. There was no significant difference in acceptability from 13.6% to 8.6% MC and between 8.9% and 11.0% MC for flour yield. Both Tax 1485 and mixed varieties followed the same trend only that they have different absolute values. The mixed varieties however had no significant difference in protein content between 19.8% and 10.2% MC. These results, therefore established that to obtain acceptable soyflour of high flour yield and protein content, moisture content of soybean after processing should be about 10% (dry basis).

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