scholarly journals First Report of Infection of Lygodium microphyllum by Puccinia lygodii, a Potential Biocontrol Agent of an Invasive Fern in Florida

Plant Disease ◽  
2005 ◽  
Vol 89 (1) ◽  
pp. 110-110 ◽  
Author(s):  
M. B. Rayamajhi ◽  
R. W. Pemberton ◽  
T. K. Van ◽  
P. D. Pratt
Keyword(s):  
Lower Surface ◽  
Positive Control ◽  
Control Agent ◽  
New Host ◽  
First Report ◽  
Central Florida ◽  
Potential Biocontrol Agent ◽  
The Family ◽  
Plastic Bag ◽  
Southern Florida

Lygodium microphyllum (Cav.) R.Br. (Old World climbing fern), in the family Schizaeaceae, is one of the most invasive (Category I in Florida) weeds in Florida. It has invaded more than 50,000 ha of wetlands and moist habitats in southern Florida and is rapidly spreading in new areas of the Everglades (3). The search and evaluation of biocontrol agents for this fern is currently in progress. Puccinia lygodii (Har.) Arth. (Uredinales) (1), previously recorded on L. volubile Sw. and L. venustum Sw. in South America (2), attacks foliage and severely damages L. japonicum Thunb. (Japanese climbing fern) vines in northern and central Florida (4). We hypothesized that since L. japonicum occurred mainly in northern and central Florida, P. lygodii did not have opportunity to interact with L. microphyllum, which primarily occurs in southern Florida. Therefore, we used two inoculation methods to test the possible pathogenicity of P. lygodii on the new host, L. microphyllum. Method-I was designed to imitate a seminatural inoculation technique in which three containerized (0.45-L capacity) L. microphyllum test plants (15- to 30-cm-high sporelings) were intermixed among a group of containerized (5.0-L capacity) P. lygodii-infected L. japonicum plants (source of inoculum) in a glasshouse. In Method-II, uredospores obtained from pustules on diseased L. japonicum foliage were adjusted to 1 × 106 uredospores/ml and then misted on three L. microphyllum sporelings (same size as in Method-I) until foliage was completely wet. The plants were then covered individually with a plastic bag for 3 days to facilitate spore germination and infection. In both methods, three L. japonicum sporelings of similar size as L. microphyllum were intermixed among diseased L. japonicum plants as a positive control. All test and infected plants were placed on 6-cm-high trays filled two-thirds with water and exposed to diffused daylight and a temperature range of 20 to 35°C in a glasshouse. These plants were monitored for the development of rust symptoms (halos and rust pustules) development for 8 weeks. Minute cinnamon flakes that developed into eruptive pustules were seen on the lower surface of the pinnules approximately 42 and 28 days after treatment initiation (in both methods) for L. microphyllum and L. japonicum (positive control), respectively. Each method was repeated twice. Dimensions (29.7 [±3.7] × 23.5 [±2.6] μm) and morphology of urediniospores from pustules on inoculated L. microphyllum were similar to those reported for P. lygodii on other host systems (1,2,4). To our knowledge, this is the first report demonstrating the infection of P. lygodii on L. microphyllum. The potential use of P. lygodii as a classical bio-control agent of L. microphyllum in southern Florida will be further investigated. References: (1) J. C. Arthur. Bull. Torrey Bot. Club 51:55, 1924. (2) J. W. McCain et al. Mycotaxon 39:281, 1990. (3) R. W. Pemberton. SIDA 20:1759, 2003. (4) M. B. Rayachhetry et al. Plant Dis. 85:232, 2000.

scholarly journals First Report of Colletotrichum acutatum Causing a Leaf Spot on Myrica cerifera in Florida

Plant Disease ◽  
2006 ◽  
Vol 90 (9) ◽  
pp. 1263-1263 ◽  
Author(s):  
S. J. Mackenzie ◽  
L. M. Takahashi ◽  
J. C. Mertely ◽  
T. E. Seijo ◽  
N. A. Peres
Keyword(s):  
Colletotrichum Acutatum ◽  
Rrna Gene ◽  
Single Spore ◽  
Myrica Cerifera ◽  
First Report ◽  
Central Florida ◽  
Potted Plants ◽  
5.8S Rrna Gene ◽  
Young Leaves ◽  
Plastic Bag

Wax myrtle (Morella cerifera (synonym Myrica cerifera) (L.) Small) is a native tree used in Florida landscapes. In the summer of 2005 and spring of 2006, small necrotic spots were observed on young leaves in two commercial nurseries in central Florida. Lesions were dark brown-to-black and eventually coalesced to form large, irregular necrotic areas. Leaves with large lesions abscised prematurely, defoliating the entire plant. Conidia formed on acervuli were observed on the surface of the largest lesions and were tentatively identified as a Colletotrichum sp. Isolations from the edges of lesions were made on potato dextrose agar (PDA) after surface disinfestation of leaf pieces in 0.6% NaOCl for 30 sec. Red chromogenic colonies developed after 5 days of incubation at 24°C. Colonies produced hyaline, oblong conidia with pointed ends averaging 14 × 4 μm and were identified as Colletotrichum acutatum J.H. Simmonds (1). The sequence from internal transcribed spacer regions 1 and 2 and the 5.8s rRNA gene of the rDNA repeat for an isolate (GenBank Accession No. DQ839609) was 100% identical to sequence from the same region of 36 C. acutatum isolates in the NCBI database. These isolates came from at least 16 different hosts, including seven ornamental hosts. There were three isolates from blueberry among the matches (Accession Nos. AB219029, AJ301911, and AJ301905), and the rDNA sequence was also identical to the sequence obtained in our laboratory for a chromogenic C. acutatum isolate from blueberry. Three single-spore isolates were tested for pathogenicity on potted plants in the greenhouse. Two young shoots were spray inoculated with a suspension (1 × 106 conidia/ml) of each isolate. Shoots were covered with a plastic bag for 24 h and maintained at 26.5°C. Two shoots were sprayed with sterile water as a control and similarly covered. All isolates produced brown spots on the youngest leaves 3 to 5 days after inoculation; no symptoms developed on control shoots. The fungus was reisolated from all inoculated shoots. To our knowledge, this is the first report of C. acutatum on wax myrtle in Florida. The disease has a potential to spread and become a significant problem for the cultivation of this species in ornamental nurseries in Florida. Reference: (1) J. H. Simmonds. Qld. J. Agric. Anim. Sci. 22:437, 1965.

scholarly journals First Report of Anthracnose Caused by Colletotrichum gloeosporioides on Gueroba in Brazil

Plant Disease ◽  
2002 ◽  
Vol 86 (1) ◽  
pp. 72-72 ◽  
Author(s):  
M. J. d'A. Charchar ◽  
J. R. N. Anjos ◽  
A. K. Akimoto
Keyword(s):  
Colletotrichum Gloeosporioides ◽  
Federal District ◽  
Palm Tree ◽  
Pathogenicity Test ◽  
Fungal Culture ◽  
First Report ◽  
Central Brazil ◽  
Tissue Sections ◽  
The Family ◽  
Plastic Bag

Gueroba (Syagrus oleracea (Mart.) Becc.), a member of the family Arecaceae, is an important native palm tree in central Brazil and has great potential as a cultivated crop and ornamental plant (1). In July 1999, anthracnose symptoms were observed on several gueroba plants growing in a field in Planaltina, Federal District. Leaves showed spots or small circular to irregular, brown-to-black necrotic lesions, which generally coalesced as symptoms progressed. Tissue sections from leaves with symptoms were plated aseptically on potato dextrose agar (PDA). A fungus was consistently isolated from leaves of symptomatic plants and was identified as Colletotrichum gloeosporioides (Penzig) Penzig & Sacc. by P. F. Cannon of CABI Bioscience, Egham, UK, where a culture (IMI 384186) has been deposited. An isolate was tested for pathogenicity by inoculating 20 plants at the two-leaf stage by placing a mycelial PDA plug from a 10-day-old fungal culture on a previously wounded leaf. Noninoculated plants served as controls. Plants were enclosed in a plastic bag and incubated for 4 days at 26 ± 2°C and ≈100% relative humidity. Ten days after inoculation, plants developed leaf lesions similar to those observed in the field. Symptoms did not develop on the control plants. C. gloeosporioides was reisolated from 95% of the inoculated leaves, completing Koch's postulates. The pathogenicity test was repeated with the same results. To our knowledge, this is the first report of C. gloeosporioides causing anthracnose on gueroba in Brazil. Reference: (1) H. Lorenzi. Page 288 in: Árvores Brasileiras. Plantarum. Nova Odessa, Brazil 1998.

scholarly journals First Report of Geranium Rust (Puccinia pelargonii-zonalis) in the State of Michoacán, México

Plant Disease ◽  
2013 ◽  
Vol 97 (12) ◽  
pp. 1660-1660 ◽  
Author(s):  
M. R. Gregorio-Cipriano ◽  
S. P. Fernández-Pavía ◽  
G. Rodríguez-Alvarado ◽  
N. Gómez-Dorantes
Keyword(s):  
Ornamental Plants ◽  
Lower Surface ◽  
The State ◽  
Post Inoculation ◽  
First Report ◽  
Light Yellow ◽  
Initial Symptoms ◽  
Plastic Bag ◽  
Pathogenicity Tests ◽  
Concentric Circles

Geranium is one of the most popular ornamental plants in México. In December 2012, rust symptoms were observed on leaves of common geranium (Pelargonium × hortorum L. H. Bailey) growing in pots in garden landscapes in Morelia, Michoacán. Dark brown pustules with chlorotic halos appeared on the lower leaf surface. A center pustule surrounded by one or more partial-to-complete concentric circles of smaller pustules was observed in each lesion. Urediniospores were globose or subglobose to ovoid, light brown, echinulated, thin-walled with two more or less conspicuous subequatorial pores, and 21 to 29 × 18 to 24 μm (25.5 × 22.8 μm average). Teliospores were not observed. Based on these characters, the rust was identified as Puccinia pelargonii-zonalis Doidge (1,2). Pathogenicity tests were conducted on three healthy P. × hortorum plants that were sprayed with water droplets containing urediniospores. The inoculated plants were covered with a plastic bag and placed in a screened house. The bags were removed after 24 h. Afterwards, the plants were maintained outside the screened house in full sun at ambient temperature (24 to 30°C in the day and 5 to 10°C at night). Initial symptoms were observed 15 to 17 days post inoculation. Symptoms appeared as small light yellow spots on the upper surface of mature leaves. Urediniospores production on the lower surface of the leaves was evident 22 to 25 days post inoculation. To our knowledge, this is the first report of P. pelargonii-zonalis in the state of Michoacán, México. Geranium rust has been previously reported only in the state of Guanajuato (2). References: (1) E. M. Doidge. Bothalia 2:1, 1926. (2) H. L. Gallegos and G. B. Cummings. Uredinales (royas) de México. Vol. 1. Culiacán, Sinaloa, México, SARH, 1981.

Survey of Biota Associated with Monochamus (Coleoptera: Cerambycidae) in Maine.

1963 ◽  
Vol 95 (1) ◽  
pp. 83-95 ◽  
Author(s):  
R. S. Soper ◽  
R. E. Olson
Keyword(s):  
Beauveria Bassiana ◽  
New Genus ◽  
Biological Control Agent ◽  
Host Record ◽  
Control Agent ◽  
New Genus And Species ◽  
New Host ◽  
New Host Record ◽  
The Family ◽  
Monochamus Notatus

AbstractThe biota associated with Monochamus species collected in Maine include species of Acarina, Diptera, Hymenoptera and Nematoda, as well as fungal and bacterial pathogens.Mites belonging to seven families and thirteen genera were found in association with the sawyers. Two species were previously unknown and have since been described as the new genus and species Longoseius cuniculus Chant of the family Digamasellidae and the new genus and species Mucroseius monochami Lindquist of the family Blattisocidae. Parasitic diptera belonged to three genera in three different families, and of these one contained a new species, Forcipomyia innom., family Ceratopogonidae, which was found as an intermittent parasite of adult Monochamus notatus and Monochamus scutellatus. This constitutes a new host record for the biting midge family. Only one hymenopterous parasite, a species of Dolichomitus was reared from a sawyer larva. The five genera of nematodes isolated from larvae, pupae, and adults of Monochamus species include specimens of the genus Hexamermis. Parasitism by this group is thought to be unusual because of the relative inaccessibility of the host. Isolates from eight fungal genera were obtained, and strains of Beauveria bassiana, and Isaria farinosa, were shown to be pathogenic. The bacteria isolated from dead sawyers included Aerobacter aerogenes, Bacillus cereus var. mycoides, and one unidentified species. Only the tachinid E. monohammi and the mite Uropoda have been previously reported of the large number of organisms found associated with sawyer beetles in this study. The fungus Beauveria bassiana proved to be the most important biological control agent.

scholarly journals First Report of Powdery Mildew Caused by Erysiphe (Golovinomyces) orontii on Veronica spicata in Italy

Plant Disease ◽  
2006 ◽  
Vol 90 (6) ◽  
pp. 831-831
Author(s):  
A. Garibaldi ◽  
D. Bertetti ◽  
D. Minerdi ◽  
M. L. Gullino
Keyword(s):  
Powdery Mildew ◽  
Its Region ◽  
Lower Surface ◽  
Pathogenicity Test ◽  
Sphaerotheca Fuliginea ◽  
First Report ◽  
Golovinomyces Orontii ◽  
The Family ◽  
Blastn Analysis ◽  
White Mycelium

Veronica spicata (spike speedwell) is a perennial garden species belonging to the family Scrophulariaceae. During the summer through fall of 2004 and 2005, severe outbreaks of a previously unknown powdery mildew were observed in several gardens near Biella (northern Italy). Upper surfaces of leaves were covered with a white mycelium and conidia, and as the disease progressed, infected leaves turned yellow and died. Very rarely was the mycelium observed on the lower surface of leaves or on petioles and flowers. Foot cell was cylindric and measured 19.2 to 25.7 × 10.8 to 14.3 μm (average 21.9 × 12.0 μm). Conidia were hyaline, ellipsoid, brought in short chains (three conidia per chain), and measured 22.2 to 40.8 × 13.6 to 21.6 μm (average 30.1 × 17.0 μm). Conidiophores measured 45.5 to 74.0 × 10.4 to 11.0 μm (average 59.4 × 10.6 μm). Fibrosin bodies were absent. Cleistothecia were never observed on the samples collected. The ITS region (internal transcribed spacer) of rDNA was amplified using the primers ITS4/ITS6 (3) and sequenced. BLASTn analysis (1) of the 504 bp obtained showed an E-value of 0.0 with Erysiphe (Golovinomyces) orontii (2). The nucleotide sequence has been assigned GenBank Accession No. DQ386696. Pathogenicity was confirmed by gently pressing diseased leaves onto leaves of five healthy Veronica spicata plants. Five noninoculated plants served as controls. Inoculated and noninoculated plants were maintained in a greenhouse where temperatures ranged between 15 and 28°C. After 15 days, typical powdery mildew symptoms developed on inoculated plants. Noninoculated plants did not show symptoms. The pathogenicity test was carried out twice. To our knowledge, this is the first report of the presence of powdery mildew on V. spicata in Italy. Sphaerotheca fuliginea has been reported as the causal agent of powdery mildew on V. spicata (4). Specimens of this disease are available at DIVAPRA Collection, University of Torino. References: (1) S. F. Altschul et al. Nucleic Acids Res. 25:3389, 1997. (2) U. Braun. Nova Hedwigia 89:166, 1987. (3) D. E. L. Cooke and J. M. Duncan. Mycol. Res. 101:667, 1997. (4) B. Ing. Mycologist 4:125, 1990.

scholarly journals Pathogenicity Assessment of Puccinia lygodii, a Potential Biological Control Agent of Lygodium japonicum in Southeastern United States

Plant Disease ◽  
2001 ◽  
Vol 85 (2) ◽  
pp. 232-232 ◽  
Author(s):  
M. B. Rayachhetry ◽  
R. W. Pemberton ◽  
L. L. Smith ◽  
R. Leahy
Keyword(s):  
United States ◽  
Biological Control ◽  
Biological Control Agent ◽  
Rust Fungus ◽  
Experimental Period ◽  
Lower Surface ◽  
The United States ◽  
Control Agent ◽  
Plastic Bag ◽  
Lygodium Japonicum

Lygodium japonicum (Thunb.) Swartz (Family, Schizaeaceae) is naturally distributed from Asia to Australia and has naturalized in the United States from Texas to the Carolinas and Florida (4). Recently, it has been declared a Category I weed (the most invasive group) by the Florida Exotic Pest Plant Council. A foliar rust fungus was observed on a population of L. japonicum growing under the canopy of a pine/hardwood forest in Gainesville, FL. The lower surfaces of almost all the pinnules (foliage) were covered with cinnamon-brown eruptive pustules. Necrotic areas developed around mature, erupted, and coalesced pustules. Severely infected foliage were wilted and dried. Microscopic observations of the pustules and spore morphology revealed these eruptive structures to be uredinia. The dimensions (24.6 [+ 2.2] × 29.7 [+ 3.5] μm) and morphology (ellipsoid or obovoid, pale cinnamon-brown, and echinulate with indistinct pores) of urediniospore were similar to those reported for Puccinia lygodii (Har.) Arth. (Uredinales) (1). Therefore, the rust was identified as P. lygodii and confirmed by J. Hennen. P. lygodii is native to South America, where it has been recorded from L. volubile and L. venustum (2). This rust was previously identified as Milesia and Uredinopsis spp. on L. japonicum from Louisiana and Florida, respectively (3). Herein, we report the performance of Koch's postulates for P. lygodii on L. japonicum. Excised foliage bearing uredinia from plants collected near Gainesville were placed in a flask, flooded with deionized distilled water, shaken vigorously for a few minutes, and the suspension strained through four layers of cheesecloth. Urediniospores suspended in the filtrate were concentrated to 1.0 × 106 spores/ml, using sedimentation technique, and then misted onto 3-week-old foliage of fully expanded fronds of four juvenile L. japonicum plants grown in pots, until the foliage were completely wet. The plants were then covered with a plastic bag and placed in dappled shade. After 3 days, the bags were removed and the water-filled containers were placed around L. japonicum plants to maintain high ambient humidity. During the remaining 4-week experimental period, the temperature and relative humidity under the shaded areas ranged from 23 to 38°C and 38 to 93%, respectively. The plants were monitored daily for development of symptoms characteristics of P. lygodii. Minute cinnamon-brown flecks appeared on the foliage 20 days after inoculation. Within 3 to 5 days, these flecked areas expanded, erupted, and formed uredinia on the lower surface of the symptomatic foliage. The morphology and size range of the uredinia and urediniospores were the same as those of the P. lygodii applied in this test. This is the first report confirming pathogenicity of P. lygodii on L. japonicum. P. lygodii may be a potential biological control agent of L. japonicum in the Southeast United States. References: (1) J. C. Arthur. Bull. Torrey Club 51:55, 1924. (2) J. F. Hennen and J. W. McCain. Mycologia 85:970–986, 1993. (3) J. W. McCain, J. F. Hennen, and Y. Ono. Mycotaxon 39:281–300, 1990. (4) R. W. Pemberton and A. P. Ferriter. Am. Fern J. 88:165–175, 1998.

scholarly journals Land-Use Effect on Olive Groves Pest Prays oleae and on Its Potential Biocontrol Agent Chrysoperla carnea

Insects ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 46
Author(s):  
João Frederico Alves ◽  
Sara Mendes ◽  
António Alves da Silva ◽  
José Paulo Sousa ◽  
Daniel Paredes
Keyword(s):  
Biological Control ◽  
Land Use ◽  
Biological Control Agent ◽  
Control Agent ◽  
Agricultural Landscapes ◽  
Chrysoperla Carnea ◽  
Land Uses ◽  
Prays Oleae ◽  
Potential Biocontrol Agent ◽  
Olive Groves

Olive growing has been intensified through the simplification of agricultural landscapes. In order to rethink the environmental drawbacks of these practices, conservation biological control techniques have been examined. In this work, Prays oleae and its natural enemy Chrysoperla carnea were monitored to account for the effects of the amount and diversity of different land-uses. We found that C. carnea showed an attraction to areas with high abundances of P. oleae but this predator did not display any affection by the different land-uses. Inversely, P. oleae abundance was lower in diverse landscapes and higher in simplified ones. Importantly, higher abundances of C. carnea were related to lower infestation levels of P. oleae in the late part of the season. These results corroborate the attraction of C. carnea to the olive moth, highlighting the potential of C. carnea as a biological control agent of this pest, assert that the promotion of land-use diversity can reduce P. oleae and confirm that landscapes dominated by olive groves can promote this pest. The present study aims at contributing to the discussion about the management of agricultural ecosystems by providing farmers with sustainable alternatives that do not have harmful effects on the environment and public health.

A checklist of bat-associated macronyssid mites (Acari: Gamasina: Macronyssidae) of Russia, with new host and geographical records

Zootaxa ◽  
2021 ◽  
Vol 4974 (3) ◽  
pp. 537-564
Author(s):  
M.V. ORLOVA ◽  
P.B. KLIMOV ◽  
O.L. ORLOV ◽  
D.G. SMIRNOV ◽  
A.V. ZHIGALIN ◽  
...  
Keyword(s):  
Russian Federation ◽  
Small Mammals ◽  
Mite Species ◽  
Russian Regions ◽  
New Host ◽  
Extensive Survey ◽  
Blood Sucking ◽  
The Family ◽  
The Russian Federation ◽  
Host Parasite

The family Macronyssidae (Acari: Mesostigmata) comprises mostly obligate blood-sucking ectoparasites of bats (Mammalia: Chiroptera) and some other animals, such as small mammals, reptiles and birds. Here, we document and curate previously known data, and, based on our extensive survey, provide this article of bat-associated macronyssid mites of the Russian Federation. We record a total of 24 mite species belonging to 4 genera (Ichoronyssus, Macronyssus, Steatonyssus, and Cryptonyssus). Twenty-seven new host-parasite associations are reported. In addition, were provide data on five major Russian regions, for which bat-associated mite records were lacking. 

scholarly journals First report of Mazocroeoides georgei price, 1936 and Mazocraeoides opisthonema Hargis, 1955 in Brazil with new synonysms (Monogenea, Mazocraeidae)

1988 ◽  
Vol 83 (4) ◽  
pp. 437-440 ◽  
Author(s):  
Anna Kohn ◽  
Cláudia Portes Santos
Keyword(s):  
Rio De Janeiro ◽  
New Host ◽  
First Report ◽  
New Synonyms ◽  
First Time ◽  
New Host Records ◽  
Janeiro State

Mazocraeoides georgei price, 1936 and mazocraeoides opisthonema Hargis, 1955 are reported for the first time in Brazil in Brevoortia aurea (Spix, 1829) and in Harengula clupeola (Cuvier, 1829) respectively, clupeid fishes from the littoral of Rio de janeiro State, which represent new host records. Mazocraeoides olentangiensis Sroufe, 1958 and mazocraeoides hargisi Price, 1961 are considered new synonyms for Mazocraeoides georgei.

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