The potential biopesticide toxicity test of Ipomoea batatas (L.) Lam (Purple Sweet Potato leaf extract) against Artemia salina Leach larvae using the Brine Shrimp Lethality Test Method

Purpose: To investigate the hepatoprotective effect of Ipomoea batatas extract against alcohol-induced liver damage in mice. Methods: Male C57BL/6 mice were randomly divided into 4 experimental groups (n = 10). Normal Group: The animals received distilled water 5 ml/kg for 7 days; Alcohol Group: The animals received alcohol 5 ml/kg of 40 % w/v alcohol for 7 days; Alcohol + Purple sweet potato leaf extract (PSPE) Group: PSPE 400 mg/kg was for 7 days. The animals received alcohol 5 ml/kg of 40 % w/v alcohol for 7 days; Alcohol + Hovenia dulcis Thunb extract (HDE) Group: HDE 400 mg/kg was for 7 days. To confirmed to the liver protection effect of PSPE, it was calculated, and the levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglyceride (TG), and total cholesterol (TC) in serum were detected. To evaluate changes of histological in alcohol-fed mice, liver tissue was determined by H&E staining. Results: Blood alcohol concentration in purple sweet potato leaf extract (PSPE) 200 mg/kg and Hovenia dulcis (H. dulcis) extract (HDE) 200 mg/kg treated group significantly decreased compared to - alcohol with water treated group (p < 0.05). Serum ALT (alanine aminotransferase) and AST (aspartate aminotransferase) were markedly reduced. Liver sections in mice stained with H&E (hematoxylin and eosin) stain to displayed the physiological changes in the liver tissue. Furthermore, the results showed that inflammatory cells increased in the alcohol group compared to the normal group, but spontaneously decreased in the PSPE or HDE-treated group. Conclusion: These results demonstrate that Ipomoea batatas may be therapeutically effective in protecting the liver from alcohol-induced hepatotoxicity and fatty liver.

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KARAKTERISASI NANOPARTIKEL KITOSAN EKSTRAK DAUN UBIJALAR (Ipomoea batatas L.) MENGGUNAKAN METODE GELASI IONIK

Alotrop ◽

10.33369/atp.v2i2.7561 ◽

2019 ◽

Vol 2 (2) ◽

Author(s):

Ade Indriani Putri ◽

Agus Sundaryono ◽

I Nyoman Chandra

Keyword(s):

Sweet Potato ◽

Ipomoea Batatas ◽

Leaf Extract ◽

Wave Number ◽

Ammonium Ion ◽

Leaf Extracts ◽

Potato Leaf ◽

Chitosan Nanoparticle ◽

Purple Sweet Potato ◽

Characterization Test

The purpose of this study is to determine and measure the results of the characterization of Chitosan nanoparticle synthetized from leaves of sweet potato (Ipomoea batatas l.) extracts . The methods used to make nanoparticles is made with Ionic Gelation method based on electrostatic interaction between the Hydroxyl Amine on Chitosan with cluster of negative charge from the polianion NaTPP with conditioned surfactant Tween 80 used as surfactant. Characterization test of nanoparticle morphology in this study obtained using the Fourier Transformation Infra Red (FTIR), and Particle Size Analyzer (PSA). Results with FTIR characterization test from the obtained Chitosan-white purple sweet potato leaf extract nanoparticles has a cluster of N-H and P = O that show the occurrence of ammonium ion interaction between Chitosan with polianion from NaTPP and sweet potato leaves. The interaction seen from wave number and intensities O-H shifting from pure Chitosan at 3425.56 cm-1 to 3427, 51 cm-1, as well as on pure Chitosan N-H absorption undergoes a shift from 1597, 06 cm-1 to becomes 1629.85-1 cm-1, which shows that there has been a cross connective between ammonium ion on Chitosan and Posphat from NaTPP and white purple sweet potato leaf extract. The results of the analysis from the PSA, is known to the average Nanokitosan particle sizes extracts from white purple sweet potato leaf extracts with the addition of NaTPP i.e. of 302.6 nm and that the aim of the research on Chitosan nanoparticle synthesis has been successfully done.

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PENGARUH EKSTRAK ETANOL DAUN UBI JALAR UNGU (Ipomoea batatas L.) TERHADAP LEUKOSIT DAN LIMFOSIT MENCIT JANTAN BALB/C YANG DI INDUKSI VAKSIN HEPATITIS B

JURNAL BIOSENSE ◽

10.36526/biosense.v4i01.1421 ◽

2021 ◽

Vol 4 (01) ◽

pp. 59-71

Author(s):

Siti Hamidatul Aliyah ◽

Desi Sagita ◽

Devi Kurnia

Keyword(s):

Immune Response ◽

Hepatitis B ◽

Sweet Potato ◽

Ipomoea Batatas ◽

Leaf Extract ◽

Control Group ◽

Immunomodulatory Activity ◽

Potato Leaf ◽

Group I ◽

Purple Sweet Potato

Abstract Background: Purple sweet potato leaves (Ipomoea batatas L.) which has been known as immunomodulatory activity, which contains chemical compounds, such as vitamins, minerals, flavonoids (anthocyanin), especially iron found in a higher proportion in the leaves. Therefore, it is necessary to study the immunomodulatory activity of the leaf extract of purple sweet potato (Ipomoea batatas L.) on the immune response in Balb/c mice who induced by hepatitis B vaccines. Method: Inducing performed at day 0 and day 15. Mice with weights ± 20 g, which has been divided into normal control group, I, II, III, IV and V (each 5 mice), respectively were given orally by the leaf extract of purple sweet potato (Ipomoea batatas L.) (doses 100 mg / kg, 200 mg / kg, 300 mg / kg, 400 mg / kg and 500 mg / kg), normal controls (CMC Na 0,5%). With the volume of giving 1% of body weight in mice, performed during the study (20th days). Blood sampling is done via the tail vein (laeralis vein), to calculate the number of leukocytes (21st days) and lymph decision to count lymphocytes (21st days). Results: The results that the leaf extract of purple sweet potato (ipomoea batatas L.), especially a dose of 500 mg / kg as immunomodulatory activity can increase the number of lymphocytes proliferation cell. Conclusion: Purple sweet potato leaf extract has activity against the increase in the number of lymphocytes cell especially on dose 500 mg/kg BW who induced by hepatitis B vaccine that can stimulated an immune response.

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The antihyperlipidaemic and hepatoprotective effect of Ipomoea batatas L. leaves extract in high-fat diet rats

International Journal of Public Health Science (IJPHS) ◽

10.11591/ijphs.v10i3.20777 ◽

2021 ◽

Vol 10 (3) ◽

pp. 558

Author(s):

Nurkhasanah Mahfudh ◽

Nanik Sulistyani ◽

Muhammad Syakbani ◽

Athifah Candra Dewi

Keyword(s):

Sweet Potato ◽

High Fat Diet ◽

Ipomoea Batatas ◽

Lipid Level ◽

Hepatoprotective Effect ◽

High Fat ◽

Potato Leaf ◽

Serum Glutamic Oxaloacetic Transaminase ◽

Liver Histopathology ◽

Purple Sweet Potato

The administration of high-fat diets can increase the body's lipid level and damage the organs. Purple sweet potato leaf (Ipomoea batatas L.) was reported as an antioxidant against free radicals. This study aimed to observe the sweet potato leaf extract's activity on decreasing lipid profile and hepatoprotective effect in high-fat diet fed rats. The treatment animals were divided into five groups, namely normal control, high-fat diet (HFD) control, the treatment group of purple sweet potato leaf extract (SPLE) doses 100 mg/kg BW, 200 mg/kg BW and 400 mg/kg BW which fed with high-fat diet for 14 days and SPLE for 28 days. After treatment was completed, the blood was collected for the detection of cholesterol, triglyceride, serum glutamic oxaloacetic transaminase (SGOT), and serum glutamic pyruvate transaminase (SGPT). After that, the animals were sacrificed, and a liver histopathology observation was conducted using Haematoxylien and Eosin staining. The result showed a significant decrease in cholesterol and triglyceride levels (p≤0.05) compared to the negative group in all treated groups. The SGOT and SGPT enzymes in all of treatment groups were also found to decrease compared with HFD control. The result was confirmed by the histopathological observations. The finding suggested the potency of SPLE for antihyperlipidaemic and hepatoprotective agent.

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Inhibition of α-Glucosidase Activity and The Toxicity of Tristaniopsis merguensis Griff. Leaf Extract

Elkawnie ◽

10.22373/ekw.v6i1.5732 ◽

2020 ◽

Vol 6 (1) ◽

pp. 67

Author(s):

Robby Gus Mahardika ◽

Occa Roanisca ◽

Fajar Indah Puspita Sari

Keyword(s):

Toxicity Test ◽

Brine Shrimp ◽

Leaf Extract ◽

Positive Control ◽

Acetone Extract ◽

Antidiabetic Activity ◽

Brine Shrimp Lethality ◽

Lc50 Value ◽

Ic50 Value ◽

Ft Ir

Abstract : This study aims to determine the antidiabetic activity and toxicity of the acetone extract of Tristaniopsis merguensis Griff leaf. The antidiabetic test was the α-glucosidase inhibition method, while the toxicity test used the Brine Shrimp Lethality Test (BSLT) method. The acetone extract possessed antidiabetic activity with an IC50 value of 8.83 ± 0.31 (μg/mL). This value is not much different from the positive control of quercetin which has an IC50 value of 6.04 ± 0.14 (μg/mL). The characteristics of the FT-IR spectrum of acetone extract showed that Tristaniopsis merguensis leaf has the groups Ar-OH (phenolic), -OH (hydroxyl), C=O (ketone) and C=C (aromatic). Based on the toxicity test, the Tristaniopsis merguensis leaf acetone extract has an LC50 value of 959.25 ppm which means that the acetone extract is toxic. Therefore, the acetone extract of Tristaniopsis merguensis might be the potential agent of antidiabetic.Abstrak : Penelitian ini bertujuan untuk mengetahui aktivitas antidiabetes dan toksisitas dari ekstrak aseton daun Tristaniopsis merguensis Griff. Uji aktivitas antidiabetes ditentukan berdasarkan metode inhibisi enzim α-glucosidase, sedangkan toksisitas ditentukan berdasarkan metode Brine Shrimp Lethality Test (BSLT). Ekstrak aseton memiliki aktivitas antidiabetes dengan nilai IC50 8,83 ± 0,31 (μg/mL). Nilai ini tidak jauh berbeda dengan kontrol positif quersetin yang memiliki nilai IC50 6,04 ± 0,14 (μg/mL). Karakteristik spektrum FT-IR ekstrak aseton menunjukkan bahwa daun Tristaniopsis merguensis memiliki gugus fungsi Ar-OH (fenolik), -OH (hidroksil), C=O (keton) dan C=C (aromatik). Berdasarkan uji toksisitas, ekstrak aseton daun Tristaniopsis merguensis memiliki nilai LC50 sebesar 959,25 ppm yang berarti bahwa ekstrak aseton bersifat toksik. Oleh karena itu, ekstrak aseton dari Tristaniopsis merguensis berpotensi untuk dijadikan agen antidiabetes.

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UJI TOKSISITAS DARI EKSTRAK LAMUN JENIS Thalassia hemprichii DARI PERAIRAN KALASEY DENGAN MENGGUNAKAN METODE BRINE SHRIMP LETHALITY TEST

JURNAL PESISIR DAN LAUT TROPIS ◽

10.35800/jplt.7.3.2019.26017 ◽

2019 ◽

Vol 7 (3) ◽

pp. 265

Author(s):

Faradila Y. Karim ◽

Nickson J Kawung ◽

Billy Th. Wagey

Keyword(s):

Cytotoxic Activity ◽

Toxicity Test ◽

Brine Shrimp ◽

Bioactive Compound ◽

Artemia Salina ◽

Probit Analysis ◽

Thalassia Hemprichii ◽

Brine Shrimp Lethality ◽

A Value ◽

Small Doses

ABSTRACTBioactive compounds that are suspected of having anticancer activity are first tested for activity by means of a toxicity test. The purpose of this test is to obtain data on the ability of the activity of a bioactive compound to kill cells at small doses so as to obtain a lethal concentration or lethal data. These two measurements are often called LC50 or LD50, concentrations that can kill 50% of test animals. This study aims to test the cytotoxic activity of Thalassia hemprichii seagrass extract using the Brine Shrimp Lethality Test (BSLT) method.The results of the study showed that the mortality of Artemia salina larvae was influenced by the concentration of the test, meaning that the higher the concentration the more test animals died. Furthermore, the results of the probit analysis contained the toxicity activity of bio active compounds from seagrasses where the LC50 was 3.95 mg / l. A substance is declared to have the potential for cytotoxic activity if it has a value of LC50 at concentrations <1000 ppm for extracts and at concentrations <30 ppm for a compound. Keywords: Cytotoxic Activity, Thalassia hemprichii, Brine shrimp Lethality Test (BSLT)

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PENGARUH JENIS PELARUT TERHADAP AKTIVITAS ANTIOKSIDAN EKSTRAK DAUN UBI JALAR PUTIH (Ipomoea batatas L) MENGGUNAKAN METODE MASERASI

Jurnal Ilmu dan Teknologi Pangan (ITEPA) ◽

10.24843/itepa.2020.v09.i04.p08 ◽

2020 ◽

Vol 9 (4) ◽

pp. 438

Author(s):

Ade Maria Kristin Gultom ◽

Ni Made Yusa ◽

Anak Agung Istri Sri Wiadnyani

Keyword(s):

Antioxidant Activity ◽

Sweet Potato ◽

Ipomoea Batatas ◽

Leaf Extract ◽

Potato Leaf ◽

Measured Variable ◽

Obvious Effect ◽

Randomized Design ◽

High Antioxidant Activity ◽

Sweet Potato Leaves

This study aims to determine the effect of solvent types on antioxidant activity of white sweet potato leaf extract (Ipomoea batatas L) and to obtain the most appropriate type of solvent to produce white sweet potato leaf extract with high antioxidant activity. The experimental design uses in this research was Completely Randomized Design (CRD) with the treatment of solvent types methanol 80%, acetone 80%, ethanol 80% and aquades. The treatment was repeated four times so obtain 16 units of the experimental. Data were analysis of variance and followed by Duncan test if the treatment has a significant effect on measured variable. The results showed that, type of solvent had an obvious effect on antioxidant activity of white sweet potato leaves. Methanol was the best solvent in extract white sweet potato leaves with followed by yield was 32.11%, total flavonoids was 226.45 mg QE / g, total tannin was 16.58 mg TAE / g, vitamin C was 119.42 mg AAE / g and antioxidant activity was 82.42%.

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