Effect of virgin coconut oil (VCO) on the physical stability of  sweet potato leaf extract (Ipomoea batatas (L.) Lam) creams and antibacterial activity test against Staphylococcus aureus ATCC 25923

This study aims to determine the effect of solvent types on antioxidant activity of white sweet potato leaf extract (Ipomoea batatas L) and to obtain the most appropriate type of solvent to produce white sweet potato leaf extract with high antioxidant activity. The experimental design uses in this research was Completely Randomized Design (CRD) with the treatment of solvent types methanol 80%, acetone 80%, ethanol 80% and aquades. The treatment was repeated four times so obtain 16 units of the experimental. Data were analysis of variance and followed by Duncan test if the treatment has a significant effect on measured variable. The results showed that, type of solvent had an obvious effect on antioxidant activity of white sweet potato leaves. Methanol was the best solvent in extract white sweet potato leaves with followed by yield was 32.11%, total flavonoids was 226.45 mg QE / g, total tannin was 16.58 mg TAE / g, vitamin C was 119.42 mg AAE / g and antioxidant activity was 82.42%.

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ANTIBACTERIAL ACTIVITY TEST OF LEAVES KECOMBRANG (NICOLAIA SPECIOSA) ETHANOLIC EXTRACTS AGAINST STAPHYLOCOCCUS AUREUS

Journal of Health ◽

10.30590/joh.v7i2.187 ◽

2020 ◽

Vol 7 (2) ◽

pp. 51-58

Author(s):

Rinda Binugraheni ◽

Ndaru Trisni Larasati

Keyword(s):

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Infectious Diseases ◽

Leaf Extract ◽

Diffusion Method ◽

Skin Infections ◽

Activity Test ◽

Ethanolic Extracts ◽

Made In ◽

Data Result

Infectious diseases that often occur in humans are skin infections. One of the bacteria that cause infection in humans is Staphylococcus aureus. One of the plants used as an ingredient in traditional medicine and used as an anti-bacterial is a kecombrang plant. This research aims to test the antibacterial activity of leaf extract of kecombrang against Staphylococcus aureus. Kecombrang leaf obtained from Pandeglang Banten. Kecombrang leaves extracted with a maceration method using 96% ethanol solvent. Extracts of leaves are made in a various concentrations (100%, 75%, 50%, and 25%). The antibacterial activity test was performed by using the diffusion method to find out the large zone of diameter are formed to inhibiting Staphylococcus aureus bacteria.The results of the antibacterial activity of the leaves extract of kecombrang to Staphylococcus aureus at 25%, 50%, 75% and 100% concentrations respectively were 12.67 mm, 14.33 mm, 15.33 mm, and 17.00 mm. The data result showed, that leaf extract of kecombrang with 100% concentration had the largest inhibiting zone diameter of 17.00 mm, among other concentrations. This suggests that kecombrang leaf extract have antibacterial activity against Staphylococcus aureus.

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EFFECT OF VIRGIN COCONUT OIL ON MYCOBACTERIUM SMEGMATIS AND STAPHYLOCOCCUS AUREUS TREATED WITH EXTRACTS OF ZANTHOXYLUM ACANTHOPODIUM FRUIT

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2021.v14i8.38219 ◽

2021 ◽

pp. 114-117

Author(s):

HEDDY JULISTIONO ◽

INTAN PERMATASARI SUSENO ◽

NURUL HANDAYANI ◽

RINI HANDAYANI ◽

PUSPA DEWI LOTULUNG

Keyword(s):

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Mycobacterium Smegmatis ◽

Coconut Oil ◽

Assay Method ◽

Bacterial Cells ◽

Virgin Coconut Oil ◽

Herbal Formulation ◽

Hexane Extracts ◽

And Staphylococcus Aureus

Objectives: To understand the potency of herbal formulation of virgin coconut oil (VCO) and andaliman (Zanthoxylum acanthopodium) fruit activity against microbes, effects of ethylene acetate and hexane extracts of fruit of andaliman on viability and ions leakages of Mycobacterium smegmatis dan Staphylococcus aureus treated with VCO has been investigated. Methods: Antibacterial activity of extracts of andaliman fruit, or VCO, or andaliman and VCO against M. smegmatis and S. aureus was investigated using MTT assay method. Membrane disruption of bacterial cells treated with the plant extract and VCO was determined by measuring potassium and sodium ions leakages using Atomic Adsorbtion Spectrophotometer. Results: VCO of 512 μg/ml did not have antibacterial activity. In M. smegmatis treated with andaliman hexane extract, presence VCO decreased both ions leakage whereas in S. aureus treated with ethyl acetate extract only sodium ion was decreased. In both microorganisms, VCO could not protect cells of both M. smegmatis and S. aureus from death caused by andaliman extracts. Conclusions: VCO prevented ions leakages of the bacteria treated with extract of andaliman but did not protects cells from death.

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Test Activities Antibakteriekstrak Ethanol Young And Old Leaves Of The Soursop(Annonamuricata L.) Against Staphylococcus aureus

Talenta Conference Series Science and Technology (ST) ◽

10.32734/st.v1i2.290 ◽

2018 ◽

Vol 1 (2) ◽

pp. 135-143 ◽

Cited By ~ 1

Author(s):

Dwi Latifah Sari ◽

Sudarmi Sudarmi ◽

Popi Patilaya

Keyword(s):

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Leaf Extract ◽

Skin Diseases ◽

Ethanol Extract ◽

Positive Control ◽

Annona Muricata ◽

Phytochemical Screening ◽

Activity Test ◽

Young Leaves

Soursop (Annona muricata L.) has been used for generations by some communities in Indonesia to treat diseases. Soursop leaves are used as an alternative treatment for cancer, by boiling them in the water and consuming it. In addition to the treatment of cancer, soursop plant is also used for the treatment of skin diseases such as boils. This study aimed to determine the antibacterial activity of ethanol extract of soursop leaves of young and old against Staphylococcus aureus, determine the content of secondary metabolites, and find out the diameter of inhibitory young leaves and old leaves of soursop in inhibiting Staphylococcus aureus. Soursop leaves were extracted by maceration using ethanol 96%. The extract obtained was then carried out to phytochemical screening. Antibacterial activity test was conducted using disc the Kirby-Bauerdiffusion. This study used 6 concentrations of 500 mg/mL, 400 mg/mL, 300 mg/mL, 200 mg/mL, 100 mg/mL, 80 mg/mL, 60 mg/mL, 40 mg/mL, 20 mg/mL, 10 mg/mL and 1 mg/mL. A positive control used soursop leaf extract of young and old while a negative one used DMSO 10%. Based on phytochemical screening, ethanol extract of soursop leaves contained alkaloids, flavonoids, saponins, tannins, steroids/triterpenoids and glycosides. The antibacterial activity of ethanol extract of leaves of young soursop was higher in inhibitory diameter than the ethanol extract of old soursop leaves. It could be seen that a concentration of 500 mg/mL was equal to 10.87 mm and 8.68 mm; a concentration of 400 mg/mL was equal to 9.15 mm and 7.3 mm; a concentration of 300 mg/mL was equal to 8.34 mm and 6.30 mm; a concentration of 200 mg/mL was equal to 8.23 mm and 7.08 mm; a concentration of 100 mg/mL was equal to 6.32 mm and 6.18 mm, and a concentration of 80 mg/mL did not leave a diameter inhibitory to bacteria Staphylococcus aureus. Young soursop leaf ethanol extract was more effective than the old soursop one against Staphylococcus aureus.

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RESPONS GLUKOSA DARAH BENIH IKAN JELAWAT (Leptobarbus hoeveni Blkr) DALAM MEDIA YANG DIBERI EKSTRAK DAUN UBI JALAR (Ipomoea batatas)

Jurnal Akuakultur Sungai dan Danau ◽

10.33087/akuakultur.v5i2.68 ◽

2020 ◽

Vol 5 (2) ◽

pp. 50

Author(s):

Nur Rizki ◽

Muhammad Sugihartono ◽

Muarofah Ghofur

Keyword(s):

Blood Glucose ◽

Sweet Potato ◽

Ipomoea Batatas ◽

Leaf Extract ◽

Potato Leaf ◽

Glucose Response ◽

Glucose Levels ◽

Extract Concentration ◽

Blood Glucose Response ◽

Blood Glucose Levels

Use of sweet potato leaf extract to determine the blood glucose response of jelawat fish (L. hoeveni, Blkr) seeds maintained in the media.This study aims to determine the optimal concentration of sweet potato leaf extract to reduce the stress level of Jelawat fish (L. hoeveni, Blkr) seeds by measuring blood glucose. In this study, four treatments were used with three replications using a concentration of 2ml / L sweet potato leaf extract, 4ml / L extract concentration, 6ml / L extract concentration, and control. The results showed that the 2ml / L extract concentration was the best because it was proven that the fish blood glucose response at every 1st, 6th, 12th hour, 24th hour, 168th hour fish (L. hoeveni, Blkr) seed blood glucose levels nettle nearly close to normal blood glucose levels in fish.

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PEMBUATAN NANOPARTIKEL-PERAK EKSTRAK DAUN UBI JALAR ORANGE (Ipomoea batatas L.) DENGAN METODE BIOREDUKSI DAN UJI AKTIVITAS TERHADAP JUMLAH TROMBOSIT Mus musculus

Alotrop ◽

10.33369/atp.v3i1.9042 ◽

2019 ◽

Vol 3 (1) ◽

Author(s):

Ririn Trinanda ◽

Agus Sundaryono ◽

Dewi Handayani

Keyword(s):

Particle Size ◽

Silver Nanoparticles ◽

Sweet Potato ◽

Mus Musculus ◽

Ipomoea Batatas ◽

Leaf Extract ◽

Scattering Method ◽

Potato Leaf ◽

Agno3 Solution ◽

Potato Extract

This study aims to make silver nanoparticles (NPP) of orange Sweet potato extract (Ipomoea batatas L.) using bioreduction method and test the effect of giving silver nanoparticles - leaf extract of Ipomoea batatas L. orange (Ipomoea batatas L.) to the number of thrombocyte from male of Mus musculus induced by aspirin. The precursor used is 1 mM AgNO3 solution and as bioreductor is an antioxidant compound in orange sweet potato extract (Ipomoea batatas L.). The ratio of AgNO3 1 mM solution volume and orange sweet potato leaf extract is 9: 1 with 2 hours time. The characteristic test again obtained NPP is done using FTIR and Particle Size Analyzer (PSA) using Dynamyc Light Scattering method (DLS). The results showed that from the PSA analysis is known the average size of NPP – I. Batatas, L leaf extract is an average of 86.0 nm with a polydispersity value (PDI) of 0.17 < 1 that indicates that the NPP-leaf extract particle size distribution It is less homogeneous and are less stable.The results show that the administration of silver nanoparticles - orange sweet potato extract (Ipomoea batatas L.) can increase the platelet count of male mice significantly Dose of 0.014 g / Kgbb of 259,600 / mm3.

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Physical Evaluation of Anti Acne Mask With Ethanol Extract of Purple Sweet Potato Leaf (Ipomoea batatas (L.) Antin-3 Varieties

Journal of Pharmacy and Science ◽

10.53342/pharmasci.v6i2.219 ◽

2021 ◽

Vol 6 (2) ◽

pp. 69-73

Author(s):

Damaranie Dipahayu ◽

Kinanti Ayu Puji Lestari

Keyword(s):

Staphylococcus Aureus ◽

Phase Separation ◽

Sweet Potato ◽

Research Method ◽

Ipomoea Batatas ◽

Ethanol Extract ◽

Physical Characteristics ◽

Potato Leaf ◽

Ph Values ◽

Purple Sweet Potato

Ethanol extract of purple sweet potato (Ipomoea batatas (L.)) leaves of Antin-3 variety contains flavonoids and polyphenols which can be used as a source of acne anti-bacterial because it can inhibit the growth of Staphylococcus aureus. This study aims to obtain data on physical characteristics, acceptability and stability of anti-acne mask formulation of 70% ethanol extract of Antin-3 leaves. The research method used were characteristic, acceptability and stability test. There were 4 types of samples tested, namely base, Antin-3 extract 5%: 10% and 20% (B: F1: F2: F3). The organoleptic results showed that the higher concentration of extract, the darker color of mask and the stronger of extract smell and the softer of consistency. The pH values (B: F1:F2: F3) were respectively 6.46; 5.78; 5.75; 5.46. The value of adhesion (B: F1: F2: F3) were respectively 1.1; 1.46; 2.17 and 4.53 seconds. The value of spreading capacity (B: F1: F2: F3) were respectively 3.89; 4.15; 4.36 and 3.45 cm. The results of the acceptability test showed that F2 was the easiest formula to spread, clean and softest sensation on the skin. The results of the mechanical and cycling test showed that the four samples had not undergo phase separation. Anti-acne mask with 70% ethanol extract of Antin-3 leaves by 10% met the physical characteristics, acceptability and stability.

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Bactericidal Potential of Extract Citrus Hystrix D.C.Leaf Powder on Staphylococcus aureus and Salmonella typhi

Jurnal Skala Kesehatan ◽

10.31964/jsk.v11i2.279 ◽

2020 ◽

Vol 11 (2) ◽

Author(s):

Elfira Maulidah ◽

Anny Thuraidah ◽

Leka Lutpiatina

Keyword(s):

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Infectious Diseases ◽

Leaf Extract ◽

Descriptive Analysis ◽

Salmonella Typhi ◽

Control Group ◽

Control Group Design ◽

Group Design ◽

Activity Test

Infectious diseases can be caused by bacteria such as Staphylococcus aureus and Salmonella typhi. Treatment of infectious diseases can use antibiotics. However, antibiotics can cause side effects on users. Kuit Lime leaf (Citrus hystrix D.C.) contains secondary metabolites such as alkaloids, steroids, triterpenoids, tannins, and flavonoids used as antibacterial. This study aimed to determine the bactericidal potential of the kuit lime leaf extract powder on Staphylococcus aureus and Salmonella typhi. The type of research used is the experiment, and the research design used is the Posttest Only Control Group Design. The antibacterial activity test used was the liquid dilution of MIC and MBC. The results showed the MIC of Staphylococcus aureus and Salmonella typhi was 180 mg/mL and 120 mg/mL, while the MBC results against Staphylococcus aureus and Salmonella typhi are 190 mg/mL and 130 mg/mL. Data analysis in this research conducted descriptively. Based on descriptive analysis, it concluded that the bactericidal potential of the powdered extract of kuit lime against Staphylococcus aureus is at a concentration of 190 mg/mL and Salmonella typhi at a concentration of 130 mg/mL.

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Protective effect of purple sweet potato leaf (Ipomoea batatas Linn Convolvulaceae) against alcohol-induced liver damage in mice

Tropical Journal of Pharmaceutical Research ◽

10.4314/tjpr.v20i2.12 ◽

2022 ◽

Vol 20 (2) ◽

pp. 301-308

Author(s):

Hyun Kang ◽

Sung-Gyu Lee

Keyword(s):

Sweet Potato ◽

Liver Damage ◽

Alanine Aminotransferase ◽

Ipomoea Batatas ◽

Leaf Extract ◽

Treated Group ◽

Potato Leaf ◽

Alcohol Group ◽

Purple Sweet Potato ◽

Hovenia Dulcis

Purpose: To investigate the hepatoprotective effect of Ipomoea batatas extract against alcohol-induced liver damage in mice. Methods: Male C57BL/6 mice were randomly divided into 4 experimental groups (n = 10). Normal Group: The animals received distilled water 5 ml/kg for 7 days; Alcohol Group: The animals received alcohol 5 ml/kg of 40 % w/v alcohol for 7 days; Alcohol + Purple sweet potato leaf extract (PSPE) Group: PSPE 400 mg/kg was for 7 days. The animals received alcohol 5 ml/kg of 40 % w/v alcohol for 7 days; Alcohol + Hovenia dulcis Thunb extract (HDE) Group: HDE 400 mg/kg was for 7 days. To confirmed to the liver protection effect of PSPE, it was calculated, and the levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglyceride (TG), and total cholesterol (TC) in serum were detected. To evaluate changes of histological in alcohol-fed mice, liver tissue was determined by H&E staining. Results: Blood alcohol concentration in purple sweet potato leaf extract (PSPE) 200 mg/kg and Hovenia dulcis (H. dulcis) extract (HDE) 200 mg/kg treated group significantly decreased compared to - alcohol with water treated group (p < 0.05). Serum ALT (alanine aminotransferase) and AST (aspartate aminotransferase) were markedly reduced. Liver sections in mice stained with H&E (hematoxylin and eosin) stain to displayed the physiological changes in the liver tissue. Furthermore, the results showed that inflammatory cells increased in the alcohol group compared to the normal group, but spontaneously decreased in the PSPE or HDE-treated group. Conclusion: These results demonstrate that Ipomoea batatas may be therapeutically effective in protecting the liver from alcohol-induced hepatotoxicity and fatty liver.

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