scholarly journals Identity and Pathogenicity of Fungi Associated with Crown and Root Rot of Dryland Winter Wheat in Azerbaijan

Plant Disease ◽  
2020 ◽  
Vol 104 (8) ◽  
pp. 2149-2157
Author(s):  
Göksel Özer ◽  
Timothy C. Paulitz ◽  
Mustafa Imren ◽  
Mehtap Alkan ◽  
Hafiz Muminjanov ◽  
...  
Keyword(s):  
Root Rot ◽  
Phylogenetic Analyses ◽  
Fusarium Culmorum ◽  
Elongation Factor ◽  
Bipolaris Sorokiniana ◽  
Specific Pcr ◽  
Wide Range ◽  
Comprehensive Survey ◽  
Crown And Root Rot ◽  
Species Specific

A comprehensive survey was performed to assess fungal populations associated with crown and root rot of wheat throughout the main wheat-growing areas of Azerbaijan. Samples were taken from 76 fields; 630 fungal strains were isolated, identified, and evaluated for pathogenicity. The identification was conducted with morphological and molecular tools such as species-specific PCR and DNA sequencing of the internal transcribed spacer (ITS) and translation elongation factor 1-α (EF1-α) loci. The fungus found in the greatest number of fields (44) was Fusarium culmorum with 192 isolates, followed by F. acuminatum. Other Fusarium spp. isolates were identified: F. equiseti, F. pseudograminearum, F. graminearum, F. incarnatum, F. avenaceum, F. hostae, F. oxysporum, F. proliferatum, F. algeriense, and F. brachygibbosum. Bipolaris sorokiniana, Curvularia spicifera, Exserohilum pedicellatum, Nigrospora oryzae, and Rhizoctonia spp. isolates were also identified, associated with underground parts of wheat. Phylogenetic analyses based on ITS and EF1-α sequences of the isolates showed that the isolates belonging to the same species were clearly separated in the dendrogram. Pathogenicity assays revealed that F. culmorum, F. pseudograminearum, and F. graminearum were most aggressive; F. avenaceum, F. hostae, F. algeriense, B. sorokiniana, C. spicifera, and R. solani isolates were moderately aggressive; C. inaequalis, E. pedicellatum, and N. oryzae were weakly aggressive; and others were nonpathogenic. The result of this study exhibited the existence of a wide range of species associated with crown and root rot of wheat in Azerbaijan. Additionally, this is the first report of F. hostae, F. algeriense, C. spicifera, C. inaequalis, and N. oryzae as pathogens on wheat in Azerbaijan. Azerbaijan is the second country after Algeria in which F. algeriense was detected.

scholarly journals Root and Crown Rot Fungi Associated with Spring, Facultative, and Winter Wheat in Turkey

Plant Disease ◽  
2008 ◽  
Vol 92 (9) ◽  
pp. 1299-1306 ◽  
Author(s):  
Berna Tunali ◽  
Julie M. Nicol ◽  
David Hodson ◽  
Zafer Uçkun ◽  
Orhan Büyük ◽  
...  
Keyword(s):  
Root Rot ◽  
Fusarium Species ◽  
Fusarium Culmorum ◽  
Bipolaris Sorokiniana ◽  
Fungal Species ◽  
Crown Rot ◽  
Gaeumannomyces Graminis ◽  
Host Susceptibility ◽  
Low Frequencies ◽  
Wide Range

The objective of this study was to determine the distribution frequency of the fungi associated with wheat (Triticum aestivum) crowns and roots in cereal producing areas of Turkey through a targeted survey of 518 commercial fields over a 2-year period. More than 26% of the fields had one or more of the fungal species commonly reported as part of the dryland root rot complex, Fusarium culmorum (14%) > Bipolaris sorokiniana (10%) > F. pseudograminearum (2%). The fungi considered to be part of the high rainfall root rot complex were found at very low frequencies: 2% for Gaeumannomyces graminis and 3% for Pythium spp. Species of Rhizoctonia were found in 22% of the fields. Several Fusarium species considered to be less or nonpathogenic to cereals were also found in high frequencies at 11% (F. oxysporum, F. chlamydosporum), 10% (F. sporotrichioides), and 8% (F. avenaceum and F. solani). The mostly random distribution of cereal root-rotting species across the survey area suggests the fungi are not distributed in any distinct agroecological relationship. As a result, the relative economic importance of a given species on wheat will be determined by a number of factors, such as their fungal pathogenicity, host susceptibility/tolerance, and the seasonal conditions. Results from this study suggest that there are a wide range of fungal species associated with root and crown tissues of wheat.

scholarly journals Wilt, Crown, and Root Rot of Common Rose Mallow (Hibiscus moscheutos) Caused by a NovelFusariumsp.

Plant Disease ◽  
2017 ◽  
Vol 101 (2) ◽  
pp. 354-358 ◽  
Author(s):  
S. L. Lupien ◽  
F. M. Dugan ◽  
K. M. Ward ◽  
K. O’Donnell
Keyword(s):  
Rna Polymerase Ii ◽  
Root Rot ◽  
Molecular Phylogenetics ◽  
Phylogenetic Analyses ◽  
Elongation Factor ◽  
Crown Rot ◽  
Root And Crown Rot ◽  
Fusarium Sp ◽  
Crown And Root Rot ◽  
Rot Disease

A new crown and root rot disease of landscape plantings of the malvaceous ornamental common rose mallow (Hibiscus moscheutos) was first detected in Washington State in 2012. The main objectives of this study were to complete Koch’s postulates, document the disease symptoms photographically, and identify the causal agent using multilocus molecular phylogenetics. Results of the pathogenicity experiments demonstrated that the Fusarium sp. could induce vascular wilt and root and crown rot symptoms on H. moscheutos ‘Luna Rose’. Maximum-likelihood and maximum-parsimony phylogenetic analyses of portions of translation elongation factor 1-α and DNA-directed RNA polymerase II largest and second-largest subunit indicated that the Hibiscus pathogen represents a novel, undescribed Fusarium sp. nested within the Fusarium buharicum species complex.

scholarly journals Susceptibility of Garden Trees and Shrubs to Armillaria Root Rot

Plant Disease ◽  
2020 ◽  
Vol 104 (2) ◽  
pp. 483-492
Author(s):  
Matthew G. Cromey ◽  
Jassy Drakulic ◽  
Elizabeth J. Beal ◽  
Ian A. G. Waghorn ◽  
Joe N. Perry ◽  
...  
Keyword(s):  
Root Rot ◽  
Integrated Management ◽  
Opportunistic Pathogen ◽  
High Index ◽  
Herbaceous Perennials ◽  
Specific Pcr ◽  
Armillaria Root Rot ◽  
Serious Disease ◽  
Species Specific ◽  
The University

Armillaria root rot (ARR) is a serious disease of woody plants caused by several species of Armillaria. Armillaria isolates from diagnostic samples received in 2017 were identified by genus- and species-specific PCR and compared with isolates from an earlier survey (2004 to 2007). The results were comparable and, therefore, were combined for further analysis. Three species were identified: Armillaria mellea (83%), A. gallica (15%), and A. ostoyae (2%). Their wide host range makes choice of resistant plants in management of the disease difficult. We used the Royal Horticultural Society diagnostic dataset of ARR records from U.K. gardens to compare the susceptibility of different host genera to the disease. The dataset was compared with an earlier experiment at the University of California. An index-based approach was used to separate genera into three categories: 77 low-index (<0.99), 37 medium-index (0.99 to 1.76), and 56 high-index (>1.76) genera were recorded. All three species were associated with both angiosperms and gymnosperms; moreover, A. ostoyae did not show the host preference for gymnosperms that has been reported elsewhere. A. gallica was particularly common on herbaceous perennials and showed a trend to occur on resistant hosts that may be under other stress, supporting its description as an opportunistic pathogen. Four monocotyledons grown as trees or shrubs in U.K. gardens had a very low ARR index according to indices associated with A. mellea and A. ostoyae. Genera in the order Myrtales were almost always low index, while those in the Saxifragales and Fagales were mostly high index. These results provide confidence in the use of host resistance as part of the integrated management of ARR.

The effect of methyl jasmonate on enzyme activities in wheat genotypes infected by the crown and root rot pathogen Fusarium culmorum

2015 ◽  
Vol 37 (11) ◽  
Author(s):  
Parastoo Motallebi ◽  
Vahid Niknam ◽  
Hassan Ebrahimzadeh ◽  
Sattar Tahmasebi Enferadi ◽  
Majid Hashemi
Keyword(s):  
Methyl Jasmonate ◽  
Enzyme Activities ◽  
Root Rot ◽  
Fusarium Culmorum ◽  
Wheat Genotypes ◽  
Crown And Root Rot

Diaporthe seed decay of soybean [Glycine max (L.) Merr.] is endemic in the United States, but new fungi are involved

Plant Disease ◽  
2020 ◽  
Author(s):  
Kristina Petrovic ◽  
Demetra Skaltsas ◽  
Lisa Castlebury ◽  
Brian Kontz ◽  
Tom Allen ◽  
...  
Keyword(s):  
United States ◽  
Glycine Max ◽  
Seed Quality ◽  
Phylogenetic Analyses ◽  
Elongation Factor ◽  
The United States ◽  
Post Inoculation ◽  
Seed Decay ◽  
Comprehensive Survey ◽  
Glycine Max L

Diaporthe seed decay can compromise seed quality in soybean [Glycine max (L.) Merr.] in the warm and humid production areas of the United States during crop maturation. In the current study, 45 isolates of Diaporthe were recovered from seed sampled from soybean fields affected by Diaporthe-associated diseases in eight U.S. states in 2017. The isolates obtained belonged to 10 species of Diaporthe based on morphology and phylogenetic analyses of the internal transcribed spacer, the partial translation elongation factor 1-alpha, and beta-tubulin gene sequences. The associated species included D. aspalathi, D. caulivora, D. kongii, D. longicolla, D. sojae, D. ueckerae, D. unshiuensis and three novel fungi, D. bacilloides, D. flavescens and D. insulistroma. One isolate each of the 10 species was examined for pathogenicity on seed of cv. ‘Sava’ under controlled conditions. Seven days post-inoculation, significant differences in the percentages of decayed seeds and seedling necrosis were observed among the isolates and the non-inoculated control (p<0.0001). While the isolates of D. bacilloides, D. longicolla, and D. ueckerae caused significantly greater percentage of decayed seeds (p<0.0001), the isolate of D. aspalathi caused the greatest seedling necrosis (p<0.0001) when compared to the non-inoculated control. The observation of new fungi causing Diaporthe seed decay suggests the need for a more comprehensive survey in the U.S. soybean producing areas since members of the genus Diaporthe appear to form a complex that causes seed decay.

Root Rot Caused by Species of Fusarium on Brassica carinata in South Dakota

2018 ◽  
Vol 19 (3) ◽  
pp. 188-192
Author(s):  
Paul N. Okello ◽  
Kristina Petrović ◽  
Brian Kontz ◽  
Shaukat Ali ◽  
Laura F. Marek ◽  
...  
Keyword(s):  
South Dakota ◽  
Root Rot ◽  
Rating Scale ◽  
Fusarium Species ◽  
Phylogenetic Analyses ◽  
Elongation Factor ◽  
The United States ◽  
Brassica Carinata ◽  
Oilseed Crop ◽  
Root Diseases

Brassica carinata is an emerging oilseed crop in the United States, and root diseases caused by Fusarium have the potential to cause yield losses in production. In this study, B. carinata plants were randomly sampled at vegetative and seed development plant stages from South Dakota State University experimental plots. Reddish-brown lesions were observed on roots of sampled plants from which F. acuminatum, F. oxysporum, F. solani, and F. sporotrichioides were recovered. The Fusarium species were identified based on morphology and phylogenetic analyses of the translation elongation factor 1-α gene region. Pathogenicity of the four Fusarium species was evaluated on five B. carinata accessions using a modified inoculum layer method in the greenhouse. At 21 days after inoculation, root rot severity caused by Fusarium on the B. carinata accessions was assessed on a rating scale of 0 to 4 and evaluated using relative treatment effects (RTEs). The F. oxysporum isolate caused significant differences in RTE (P = 0.01) among the B. carinata accessions. However, there were no significant differences in RTE among the B. carinata accessions in response to F. acuminatum (P = 0.82), F. solani (P = 0.76), and F. sporotrichioides (P = 0.47) isolates.

scholarly journals Prolixibacter bellariivorans gen. nov., sp. nov., a sugar-fermenting, psychrotolerant anaerobe of the phylum Bacteroidetes, isolated from a marine-sediment fuel cell

2007 ◽  
Vol 57 (4) ◽  
pp. 701-707 ◽  
Author(s):  
Dawn E. Holmes ◽  
Kelly P. Nevin ◽  
Trevor L. Woodard ◽  
Aaron D. Peacock ◽  
Derek R. Lovley
Keyword(s):  
16S Rrna ◽  
Phylogenetic Analyses ◽  
Elongation Factor ◽  
Amino Acid Sequences ◽  
Rrna Gene ◽  
Acid Fermentation ◽  
Mixed Acid ◽  
Phylogenetic Cluster ◽  
Wide Range ◽  
Novel Genus And Species

A Gram-negative, non-motile, filamentous, rod-shaped, non-spore-forming bacterium (strain F2T) was isolated from the surface of an electricity-harvesting electrode incubated in marine sediments. Strain F2T does not contain c-type cytochromes, flexirubin or carotenoids. It is a facultative anaerobe that can ferment sugars by using a mixed acid fermentation pathway and it can grow over a wide range of temperatures (4–42 °C). The DNA G+C (44.9 mol%) content and chemotaxonomic characteristics (major fatty acids, a-15 : 0 and 15 : 0) were consistent with those of species within the phylum Bacteroidetes. Phylogenetic analysis of the 16S rRNA nucleotide and elongation factor G amino acid sequences indicated that strain F2T represents a unique phylogenetic cluster within the phylum Bacteroidetes. On the basis of 16S rRNA gene sequence phylogeny, the closest relative available in pure culture, Alkaliflexus imshenetskii, is only 87.5 % similar to strain F2T. Results from physiological, biochemical and phylogenetic analyses showed that strain F2T should be classified as a novel genus and species within the phylum Bacteroidetes, for which the name Prolixibacter bellariivorans gen. nov., sp. nov. is proposed. The type strain is F2T (=ATCC BAA-1284T=JCM 13498T).

scholarly journals Root rot grain crops on Cereals caused by the phytopathogenic fungi

2018 ◽  
Vol 245 ◽  
pp. 11006 ◽  
Author(s):  
Nadezhda Grebenikova ◽  
Alexander Korshunov ◽  
Vasily Rud’ ◽  
Ivan Savchenko ◽  
Marcia Marques
Keyword(s):  
Causative Agent ◽  
Root Rot ◽  
Fusarium Culmorum ◽  
Bipolaris Sorokiniana ◽  
Phytopathogenic Fungi ◽  
Grain Crops ◽  
Root Rots

Researching the special and infraspecial structure in causative agent populations of the most dangerous diseases root rots of cereals in various regions of the Russjan Federation has been carried out. The defeat of cereals root rots old in Russia causes in the a complex patogenic. More often meet Fusarium culmorum (W.G.Sm.) Sacc., F. oxysporum (Schlecht.) Snyd.et Hans., F. heterosporum Nees., F. sporotrichiella nom.nov. Bilai F. gibbosum App.et Wr.emend Bilai., F. avenaceum (Fr.) Sacc. and Bipolaris sorokiniana (Sacc.) Shoem., Alternaria spp.

scholarly journals Botryosphaeriaceae Species Associated With Cankers and Dieback Symptoms of Acacia mangium and Pinus caribaea var. hondurensis in Venezuela

Plant Disease ◽  
2016 ◽  
Vol 100 (12) ◽  
pp. 2455-2464 ◽  
Author(s):  
J. R. Úrbez-Torres ◽  
F. Castro-Medina ◽  
S. R. Mohali ◽  
W. D. Gubler
Keyword(s):  
Dna Sequences ◽  
Tree Species ◽  
Phylogenetic Analyses ◽  
Elongation Factor ◽  
Acacia Mangium ◽  
Forest Tree ◽  
Pinus Caribaea ◽  
Internal Transcribed Spacer Region ◽  
Forest Tree Species ◽  
Wide Range

Several species in the Botryosphaeriaceae family cause wood stain, cankers, and dieback of trunks and branches in a wide range of forest tree species. The aim of this study was to characterize the botryosphaeriaceous fungi associated with decline symptoms observed in Acacia mangium and Pinus caribaea var. hondurensis, two economically important forest tree species grown in commercial plantations in Venezuela. Fungi isolated from symptomatic samples collected from both hosts in commercial sites were identified based on their morphology and DNA sequences of the internal transcribed spacer region (ITS1-5.8S-ITS2) and part of the β-tubulin and translation elongation factor 1-α genes. Lasiodiplodia theobromae and L. venezuelensis were routinely isolated from A. mangium and P. caribaea var. hondurensis. Additionally, the novel species Diplodia guayanensis was isolated and characterized from symptomatic and asymptomatic tissues of A. mangium. Multigene phylogenetic analyses along with restriction fragment length polymorphism studies further supported the identification of these species. A pathogenicity study was conducted under natural conditions and 12 weeks after inoculation all Botryosphaeriaceae spp. were shown to be highly virulent on A. mangium. Contrary, no lesions were observed in the wood of P. caribaea var. hondurensis when inoculated with L. theobromae and L. venezuelensis. However, both species were consistently reisolated from the asymptomatic tissue beyond the inoculation point. This study contributes to a better understand the role that species in the Botryosphaeriaceae play on disease symptoms and dieback of A. mangium and P. caribaea var. hondurensis from plantations in eastern Venezuela.

INTERRELATIONSHIPS BETWEEN PLANT AGE, ROOT-ROT INFECTION, AND COLD HARDINESS IN WINTER WHEAT

1960 ◽  
Vol 38 (4) ◽  
pp. 601-611 ◽  
Author(s):  
J. E. Andrews ◽  
J. S. Horricks ◽  
D. W. A. Roberts
Keyword(s):  
Winter Wheat ◽  
Root Rot ◽  
Cold Hardiness ◽  
Fusarium Culmorum ◽  
Field Studies ◽  
Bipolaris Sorokiniana ◽  
Plant Age ◽  
Cold Injury ◽  
Direct Result ◽  
Cold Hardy

The effect of plant age and root rot caused by Bipolaris sorokiniana and Fusarium culmorum on the cold hardiness of winter wheat and the effect of cold injury on root-rot infection were studied during 3 years at Lethbridge, Alberta. The oldest and youngest plants were less cold hardy than those of an intermediate age. Root-rot damage predisposed plants to cold injury and, in turn, cold injury predisposed plants to root-rot damage. Winter wheat inoculated with B. sorokiniana and F. culmorum and seeded at the earliest dates had recovered from infection before exposure to the freezing treatment and, apparently because of delayed development, was more cold hardy than uninoculated wheat seeded on the same date. Results from cold-chamber and field studies indicated that the severe root-rot damage often observed in nearly mature winter wheat seeded early the previous year is not a direct result of heavy infection at or near the time of seeding but is secondary to winter injury that often occurs in early-seeded winter wheat.

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