scholarly journals Distribution and Characterization of Podosphaera macularis Virulent on Hop Cultivars Possessing R6-Based Resistance to Powdery Mildew

Plant Disease ◽  
2016 ◽  
Vol 100 (6) ◽  
pp. 1212-1221 ◽  
Author(s):  
Sierra N. Wolfenbarger ◽  
Stephen T. Massie ◽  
Cynthia Ocamb ◽  
Emily B. Eck ◽  
Gary G. Grove ◽  
...  
Keyword(s):  
United States ◽  
Powdery Mildew ◽  
Mating Type ◽  
Resistance Genes ◽  
Severe Disease ◽  
The United States ◽  
Fitness Cost ◽  
Eastern United States ◽  
Host Genotype ◽  
Podosphaera Macularis

Host resistance, both quantitative and qualitative, is the preferred long-term approach for disease management in many pathosystems, including powdery mildew of hop (Podosphaera macularis). In 2012, an epidemic of powdery mildew occurred in Washington and Idaho on previously resistant cultivars whose resistance was putatively based on the gene designated R6. In 2013, isolates capable of causing severe disease on cultivars with R6-based resistance were confirmed in Oregon and became widespread during 2014. Surveys of commercial hop yards during 2012 to 2014 documented that powdery mildew is now widespread on cultivars possessing R6 resistance in Washington and Oregon, and the incidence of disease is progressively increasing. Pathogenic fitness, race, and mating type of R6-virulent isolates were compared with isolates of P. macularis lacking R6 virulence. All isolates were positive for the mating type idiomorph MAT1-1 and were able to overcome resistance genes Rb, R3, and R5 but not R1 or R2. In addition, R6-virulent isolates were shown to infect differential cultivars reported to possess the R6 gene and also the R4 gene, although R4 has not yet been broadly deployed in the United States. R6-virulent isolates were not detected from the eastern United States during 2012 to 2015. In growth chamber studies, R6-virulent isolates of P. macularis had a significantly longer latent period and produced fewer lesions on plants with R6 as compared with plants lacking R6, indicating a fitness cost to the fungus. R6-virulent isolates also produced fewer conidia when compared with isolates lacking R6 virulence, independent of whether the isolates were grown on a plant with or without R6. Thus, it is possible that the fitness cost of R6 virulence occurs regardless of host genotype. In field studies, powdery mildew was suppressed by at least 50% on plants possessing R6 as compared with those without R6 when coinoculated with R6-virulent and avirulent isolates. R6 virulence in P. macularis appears to be race specific and, at this time, imposes a measurable fitness penalty on the fungus. Resistance genes R1 and R2 appear to remain effective against R6-virulent isolates of P. macularis in the U.S. Pacific Northwest.

Comparative Susceptibility of Plants Native to the Appalachian Range of the United States to Inoculation with Phytophthora ramorum

2007 ◽  
Vol 8 (1) ◽  
pp. 12 ◽  
Author(s):  
Robert G. Linderman ◽  
Patricia B. de Sá ◽  
E. Anne Davis
Keyword(s):  
United States ◽  
Plant Species ◽  
Mating Type ◽  
Management Practices ◽  
The United States ◽  
Phytophthora Ramorum ◽  
Eastern United States ◽  
Comparative Susceptibility ◽  
Climatic Characteristics ◽  
Inoculum Type

Phytophthora ramorum, cause of sudden oak death of trees or ramorum blight of other plant species, has an ever-increasing host range. Some geographic regions are considered to be at high risk of becoming infested with the pathogen, possibly causing plant mortality such as seen in native habitats of California and Oregon. One such region is the Appalachian range of the eastern United States, where known susceptible plants occur and climatic characteristics appear favorable for infections by this pathogen. We collected foliage of a range of plant species native to Appalachia in Kentucky during two summer seasons, and the foliage was shipped to Oregon for inoculation with P. ramorum to determine relative susceptibility. Leaves were needle-wounded and inoculated with either mycelium agar plugs or sporangia of a North American (A2 mating type) or European (A1 mating type) isolate. After 14 days incubation at 20°C in moist boxes, lesions caused by either inoculum type or isolate generally were comparable using digital photos and ASSESS software. Some genera, species, and cultivars within species were highly susceptible, while others were moderately susceptible or not susceptible. These results provide a basis for regional surveyors to select target hosts and to generate survey and management practices for nursery and forest areas. Accepted for publication 24 April 2007. Published 17 September 2007.

scholarly journals Transcriptome-Derived Amplicon Sequencing Markers Elucidate the U.S. Podosphaera macularis Population Structure Across Feral and Commercial Plantings of Humulus lupulus

2020 ◽  
pp. PHYTO-07-20-029
Author(s):  
William A. Weldon ◽  
Brian J. Knaus ◽  
Niklaus J. Grünwald ◽  
Joshua S. Havill ◽  
Mary H. Block ◽  
...  
Keyword(s):  
United States ◽  
Pacific Northwest ◽  
Mating Type ◽  
High Throughput ◽  
Plant Pathogens ◽  
Amplicon Sequencing ◽  
The United States ◽  
Humulus Lupulus ◽  
Geographical Regions ◽  
Podosphaera Macularis

Obligately biotrophic plant pathogens pose challenges in population genetic studies due to their genomic complexities and elaborate culturing requirements with limited biomass. Hop powdery mildew (Podosphaera macularis) is an obligately biotrophic ascomycete that threatens sustainable hop production. P. macularis populations of the Pacific Northwest (PNW) United States differ from those of the Midwest and Northeastern United States, lacking one of two mating types needed for sexual recombination and harboring two strains that are differentially aggressive on the cultivar Cascade and able to overcome the Humulus lupulus R-gene R6 (V6), respectively. To develop a high-throughput marker platform for tracking the flow of genotypes across the United States and internationally, we used an existing transcriptome of diverse P. macularis isolates to design a multiplex of 54 amplicon sequencing markers, validated across a panel of 391 U.S. samples and 123 international samples. The results suggest that P. macularis from U.S. commercial hop yards form one population closely related to P. macularis of the United Kingdom, while P. macularis from U.S. feral hop locations grouped with P. macularis of Eastern Europe. Included in this multiplex was a marker that successfully tracked V6-virulence in 65 of 66 samples with a confirmed V6-phenotype. A new qPCR assay for high-throughput genotyping of P. macularis mating type generated the highest resolution distribution map of P. macularis mating type to date. Together, these genotyping strategies enable the high-throughput and inexpensive tracking of pathogen spread among geographical regions from single-colony samples and provide a roadmap to develop markers for other obligate biotrophs.

scholarly journals Occurrence and Distribution of Mating Types of Pseudoperonospora cubensis in the United States

2017 ◽  
Vol 107 (3) ◽  
pp. 313-321 ◽  
Author(s):  
Anna Thomas ◽  
Ignazio Carbone ◽  
Yigal Cohen ◽  
Peter S. Ojiambo
Keyword(s):  
United States ◽  
South Carolina ◽  
Downy Mildew ◽  
Mating Type ◽  
Severe Disease ◽  
The United States ◽  
Pseudoperonospora Cubensis ◽  
Mating Types ◽  
Cucurbit Downy Mildew ◽  
Golden Yellow

During the past two decades, a resurgence of cucurbit downy mildew has occurred around the world, resulting in severe disease epidemics. In the United States, resurgence of the disease occurred in 2004 and several hypotheses, including introduction of a new genetic recombinant or pathotype of the pathogen, have been suggested as potential causes for this resurgence. Occurrence and distribution of mating types of Pseudoperonospora cubensis in the United States were investigated using 40 isolates collected from cucurbits across 11 states from 2005 to 2013. Pairing of unknown isolates with known mating-type tester strains on detached leaves of cantaloupe or cucumber resulted in oospore formation 8 to 10 days after inoculation. Isolates differed in their ability to form oospores across all coinoculation pairings, with oospore numbers ranging from 280 to 1,000 oospores/cm2 of leaf tissue. Oospores were hyaline to golden-yellow, spherical, and approximately 36 μm in diameter. Of the 40 isolates tested, 24 were found to be of the A1 mating type, while 16 were of the A2 mating type. Mating type was significantly (P < 0.0001) associated with host type, whereby all isolates collected from cucumber were of the A1 mating type, while isolates from squash and watermelon were of the A2 mating type. Similarly, mating type was significantly (P = 0.0287) associated with geographical region, where isolates from northern-tier states of Michigan, New Jersey, New York, and Ohio were all A1, while isolates belonging to either A1 or A2 mating type were present in equal proportions in southern-tier states of Alabama, Florida, Georgia, North Carolina, South Carolina, and Texas. Viability assays showed that oospores were viable and, on average, approximately 40% of the oospores produced were viable as determined by the plasmolysis method. This study showed that A1 and A2 mating types of P. cubensis are present and the pathogen could potentially reproduce sexually in cucurbits within the United States. In addition, the production of viable oospores reported in this study suggests that oospores could have an important role in the biology of P. cubensis and could potentially influence the epidemiology of cucurbit downy mildew in the United States.

scholarly journals Presence of Plasmid-Mediated Quinolone Resistance in Klebsiella pneumoniae Isolates Possessing blaKPC in the United States

2008 ◽  
Vol 52 (7) ◽  
pp. 2680-2682 ◽  
Author(s):  
Andrea Endimiani ◽  
Lenore L. Carias ◽  
Andrea M. Hujer ◽  
Christopher R. Bethel ◽  
Kristine M. Hujer ◽  
...  
Keyword(s):  
United States ◽  
Klebsiella Pneumoniae ◽  
Resistance Genes ◽  
The United States ◽  
Quinolone Resistance ◽  
Conjugative Plasmid ◽  
Eastern United States

ABSTRACT The presence of plasmid-mediated quinolone resistance genes [i.e., qnrA, qnrB, qnrS, aac(6′)-Ib-cr, and qepA] was evaluated among 42 bla KPC-containing Klebsiella pneumoniae isolates collected in the eastern United States. One isolate carried the bla KPC-3 and qnrB19 genes on the same conjugative plasmid, whereas another carried the bla KPC-3 and qnrA1 genes on separate plasmids.

scholarly journals UPDATE ON POWDERY MILDEW RESISTANCE SCREENING IN WATERMELON

HortScience ◽  
2005 ◽  
Vol 40 (3) ◽  
pp. 872c-872
Author(s):  
Angela R. Davis ◽  
Todd C. Wehner ◽  
Amnon Levi ◽  
Stephen R. King
Keyword(s):  
United States ◽  
South Carolina ◽  
Powdery Mildew ◽  
Resistance Genes ◽  
Citrullus Lanatus ◽  
Germplasm Collection ◽  
The United States ◽  
The Past ◽  
Race 1 ◽  
Race 2

Powdery mildew has been reported on Citrullus lanatus in Africa and Europe for the past 9 years, and in the United States for the past 6 years. During this time, it has occurred in the main watermelon production areas in the U.S. and has been documented in nine states (South Carolina, Georgia, Florida, Oklahoma, Texas, Maryland, New York, Arizona, and California). This is of great concern to the watermelon industry since powdery mildew is difficult to control and can have a severe impact on yield and fruit quality due to loss of photosynthetic area and sunscald. Finding resistant C. lanatus germplasm is needed for the development of commercial varieties containing this resistance. This report summarized the status of an ongoing project to screen the entire USDA–ARS C. lanatus germplasm collection. Currently, the collection is being screened for race 1 and race 2 Podosphaera xanthii (syn. Sphaerotheca fuliginea auct. p.p.), the causal agent of powdery mildew in C. lanatus in the United States. Resistance genes appear to exist for both races and the genes conferring resistance to race 1 appear to be different than race 2 resistance genes. Allelism tests are currently in process to determine the number of resistance genes present.

scholarly journals Population Diversity and Structure of Podosphaera macularis in the Pacific Northwestern United States and Other Populations

2020 ◽  
Vol 110 (5) ◽  
pp. 1105-1116
Author(s):  
David H. Gent ◽  
Briana J. Claassen ◽  
David M. Gadoury ◽  
Niklaus J. Grünwald ◽  
Brian J. Knaus ◽  
...  
Keyword(s):  
United States ◽  
Powdery Mildew ◽  
Population Diversity ◽  
Western United States ◽  
Wild Plants ◽  
Eastern United States ◽  
The Pacific ◽  
Differential Cultivars ◽  
Pathogen Populations ◽  
Podosphaera Macularis

Powdery mildew, caused by Podosphaera macularis, is one of the most important diseases of hop. The disease was first reported in the Pacific Northwestern United States, the primary hop-growing region in this country, in the mid-1990s. More recently, the disease has reemerged in newly planted hopyards of the eastern United States, as hop production has expanded to meet demands of local craft brewers. The spread of strains virulent on previously resistant cultivars, the paucity of available fungicides, and the potential introduction of the MAT1-2 mating type to the western United States, all threaten sustainability of hop production. We sequenced the transcriptome of 104 isolates of P. macularis collected throughout the western United States, eastern United States, and Europe to quantify genetic diversity of pathogen populations and elucidate the possible origins of pathogen populations in the western United States. Discriminant analysis of principal components grouped isolates within three to five geographic populations, dependent on stringency of grouping criteria. Isolates from the western United States were phenotyped and categorized into one of three pathogenic races based on disease symptoms generated on differential cultivars. Western U.S. populations were clonal, irrespective of pathogenic race, and grouped with isolates originating from Europe. Isolates originating from wild hop plants in the eastern United States were genetically differentiated from all other populations, whereas isolates from cultivated hop plants in the eastern United States mostly grouped with isolates originating from the west, consistent with origins from nursery sources. Mating types of isolates originating from cultivated western and eastern U.S. hop plants were entirely MAT1-1. In contrast, a 1:1 ratio of MAT1-1 and MAT1-2 was observed with isolates sampled from wild plants or Europe. Within the western United States a set of highly differentiated loci were identified in P. macularis isolates associated with virulence to the powdery mildew R-gene R6. The weight of genetic and phenotypic evidence suggests a European origin of the P. macularis populations in the western United States, followed by spread of the pathogen from the western United States to re-emergent production regions in the eastern United States. Furthermore, R6 compatibility appears to have been selected from an extant isolate within the western United States. Greater emphasis on sanitation measures during propagation and quarantine policies should be considered to limit further spread of novel genotypes of the pathogen, both between and within production areas.

scholarly journals UPDATE ON POWDERY MILDEW RESISTANCE SCREENING IN WATERMELON

HortScience ◽  
2005 ◽  
Vol 40 (3) ◽  
pp. 871a-871
Author(s):  
Angela R. Davis ◽  
Todd C. Wehner ◽  
Amnon Levi ◽  
Stephen R. King
Keyword(s):  
United States ◽  
South Carolina ◽  
Powdery Mildew ◽  
Resistance Genes ◽  
Citrullus Lanatus ◽  
Germplasm Collection ◽  
The United States ◽  
The Past ◽  
Race 1 ◽  
Race 2

Powdery mildew has been reported on Citrullus lanatus in Africa and Europe for the past nine years, and in the United States for the past 6 years. During this time, it has occurred in the main watermelon production areas in the U.S. and has been documented in nine states (South Carolina, Georgia, Florida, Oklahoma, Texas, Maryland, New York, Arizona, and California). This is of great concern to the watermelon industry since powdery mildew is difficult to control and can have a severe impact on yield and fruit quality due to loss of photosynthetic area and sunscald. Finding resistant C. lanatus germplasm is needed for the development of commercial varieties containing this resistance. This report summarized the status of an ongoing project to screen the entire USDA–ARS C. lanatus germplasm collection. Currently, the collection is being screened for race 1 and race 2 Podosphaera xanthii (syn. Sphaerotheca fuliginea auct. p.p.), the causal agent of powdery mildew in C. lanatus in the United States. Resistance genes appear to exist for both races and the genes conferring resistance to race 1 appear to be different than race 2 resistance genes. Allelism tests are currently in process to determine the number of resistance genes present.

Virulence Differences in Blumeria graminis f. sp. tritici from the Central and Eastern United States

2018 ◽  
Vol 108 (3) ◽  
pp. 402-411 ◽  
Author(s):  
Christina Cowger ◽  
Lucky Mehra ◽  
Consuelo Arellano ◽  
Emily Meyers ◽  
J. Paul Murphy
Keyword(s):  
United States ◽  
Spatial Distribution ◽  
Powdery Mildew ◽  
Isolation By Distance ◽  
The United States ◽  
Blumeria Graminis ◽  
Soft Wheat ◽  
Eastern United States ◽  
Mildew Resistance ◽  
Wide Range

Wheat powdery mildew is a disease of global importance that occurs across a wide geographic area in the United States. A virulence survey of Blumeria graminis f. sp. tritici, the causal agent, was conducted by sampling 36 wheat fields in 15 U.S. states in the years 2013 and 2014. Using a hierarchical sampling protocol, isolates were derived from three separated plants at each of five separated sites within each field in order to assess the spatial distribution of pathotypes. In total, 1,017 isolates from those fields were tested individually on single-gene differential cultivars containing a total of 21 powdery mildew resistance (Pm) genes. Several recently introgressed mildew resistance genes from wild wheat relatives (Pm37, Pm53, MlAG12, NCAG13, and MlUM15) exhibited complete or nearly complete resistance to all local B. graminis f. sp. tritici populations from across the sampled area. One older gene, Pm4b, also retained at least some efficacy across the sampled area. The B. graminis f. sp. tritici population sampled from Arkansas and Missouri, on the western edge of the eastern soft red winter wheat region, had virulence profiles more similar to other soft wheat mildew populations than to the geographically closer population from hard wheat fields in the Plains states of Oklahoma, Nebraska, and Kansas. The Plains population differed in that it was avirulent to several Pm genes long defeated in the soft-wheat-growing areas. Virulence complexity was greatest east of the Mississippi River, and diminished toward the west. Several recently introgressed Pm genes (Pm25, Pm34, Pm35, and NCA6) that are highly effective against mildew in the field in North Carolina were unexpectedly susceptible to eastern-U.S. B. graminis f. sp. tritici populations in detached-leaf tests. Sampled fields displayed a wide range of pathotype diversity and spatial distribution, suggesting that epidemics are caused by varying numbers of pathotypes in all regions. The research confirmed that most long-used Pm genes are defeated in the eastern United States, and the U.S. B. graminis f. sp. tritici population has different virulence profiles in the hard- and soft-wheat regions, which are likely maintained by host selection, isolation by distance, and west-to-east gene flow.

System-wide seismic vulnerability of aging multiple-span multiple-girder bridges in low-to-moderate seismic hazard regions

2021 ◽  
Vol 37 (1_suppl) ◽  
pp. 1626-1651
Author(s):  
John E Lens M.EERI ◽  
Mandar M Dewoolkar ◽  
Eric M Hernandez M.EERI
Keyword(s):  
United States ◽  
Seismic Hazard ◽  
Cross Sections ◽  
Seismic Vulnerability ◽  
Time History ◽  
The United States ◽  
National Database ◽  
Eastern United States ◽  
Girder Bridges ◽  
The Status

This article describes the approach, methods, and findings of a quantitative analysis of the seismic vulnerability in low-to-moderate seismic hazard regions of the Central and Eastern United States for system-wide assessment of typical multiple span bridges built in the 1950s through the 1960s. There is no national database on the status of seismic vulnerability of bridges, and thus no means to estimate the system-wide damage and retrofit costs for bridges. The study involved 380 nonlinear analyses using actual time-history records matched to four representative low-to-medium hazard target spectra corresponding with peak ground accelerations from approximately 0.06 to 0.3 g. Ground motions were obtained from soft and stiff site seismic classification locations and applied to models of four typical multiple-girder with concrete bent bridges. Multiple-girder bridges are the largest single category, comprising 55% of all multiple span bridges in the United States. Aging and deterioration effects were accounted for using reduced cross-sections representing fully spalled conditions and compared with pristine condition results. The research results indicate that there is an overall low likelihood of significant seismic damage to these typical bridges in such regions, with the caveat that certain bridge features such as more extensive deterioration, large skews, and varied bent heights require bridge-specific analysis. The analysis also excludes potential damage resulting from liquefaction, flow-spreading, or abutment slumping due to weak foundation or abutment soils.

scholarly journals Pathogenic Variation of Phakopsora pachyrhizi Isolates on Soybean in the United States from 2006 to 2009

Plant Disease ◽  
2012 ◽  
Vol 96 (1) ◽  
pp. 75-81 ◽  
Author(s):  
M. Twizeyimana ◽  
G. L. Hartman
Keyword(s):  
United States ◽  
Resistance Genes ◽  
The United States ◽  
Soybean Rust ◽  
Phakopsora Pachyrhizi ◽  
Soybean Genotype ◽  
Pathogenic Variation ◽  
Differential Set ◽  
Soybean Genotypes ◽  
Soybean Leaves

The introduction of Phakopsora pachyrhizi, the cause of soybean rust, into the United States is a classic case of a pathogen introduction that became established in a new geographical region overwintering on a perennial host (kudzu, Pueraria lobata). The objective of our study was to classify the pathogenic variation of P. pachyrhizi isolates collected in the United States, and to determine the spatial and temporal associations. In total, 72 isolates of P. pachyrhizi collected from infected kudzu and soybean leaves in the United States were purified, then established and increased on detached soybean leaves. These isolates were tested for virulence and aggressiveness on a differential set of soybean genotypes that included six genotypes with known resistance genes (Rpp), one resistant genotype without any known characterized resistance gene, and a susceptible genotype. Three pathotypes were identified among the 72 U.S. P. pachyrhizi isolates based on the virulence of these isolates on the genotypes in the differential set. Six aggressiveness groups were established based on sporulating-uredinia production recorded for each isolate on each soybean genotype. All three pathotypes and all six aggressiveness groups were found in isolates collected from the southern region and from both hosts (kudzu or soybean) in 2008. Shannon's index based on the number of pathotypes indicated that isolates from the South region were more diverse (H = 0.83) compared with the isolates collected in other regions. This study establishes a baseline of pathogenic variation of P. pachyrhizi in the United States that can be further compared with variation reported in other regions of the world and in future studies that monitor P. pachyrhizi virulence in association to deployment of rust resistance genes.

Sign in / Sign up

Export Citation Format

Share Document