Comparative Susceptibility of Plants Native to the Appalachian Range of the United States to Inoculation with Phytophthora ramorum

2007 ◽  
Vol 8 (1) ◽  
pp. 12 ◽  
Author(s):  
Robert G. Linderman ◽  
Patricia B. de Sá ◽  
E. Anne Davis
Keyword(s):  
United States ◽  
Plant Species ◽  
Mating Type ◽  
Management Practices ◽  
The United States ◽  
Phytophthora Ramorum ◽  
Eastern United States ◽  
Comparative Susceptibility ◽  
Climatic Characteristics ◽  
Inoculum Type

Phytophthora ramorum, cause of sudden oak death of trees or ramorum blight of other plant species, has an ever-increasing host range. Some geographic regions are considered to be at high risk of becoming infested with the pathogen, possibly causing plant mortality such as seen in native habitats of California and Oregon. One such region is the Appalachian range of the eastern United States, where known susceptible plants occur and climatic characteristics appear favorable for infections by this pathogen. We collected foliage of a range of plant species native to Appalachia in Kentucky during two summer seasons, and the foliage was shipped to Oregon for inoculation with P. ramorum to determine relative susceptibility. Leaves were needle-wounded and inoculated with either mycelium agar plugs or sporangia of a North American (A2 mating type) or European (A1 mating type) isolate. After 14 days incubation at 20°C in moist boxes, lesions caused by either inoculum type or isolate generally were comparable using digital photos and ASSESS software. Some genera, species, and cultivars within species were highly susceptible, while others were moderately susceptible or not susceptible. These results provide a basis for regional surveyors to select target hosts and to generate survey and management practices for nursery and forest areas. Accepted for publication 24 April 2007. Published 17 September 2007.

scholarly journals Distribution and Characterization of Podosphaera macularis Virulent on Hop Cultivars Possessing R6-Based Resistance to Powdery Mildew

Plant Disease ◽  
2016 ◽  
Vol 100 (6) ◽  
pp. 1212-1221 ◽  
Author(s):  
Sierra N. Wolfenbarger ◽  
Stephen T. Massie ◽  
Cynthia Ocamb ◽  
Emily B. Eck ◽  
Gary G. Grove ◽  
...  
Keyword(s):  
United States ◽  
Powdery Mildew ◽  
Mating Type ◽  
Resistance Genes ◽  
Severe Disease ◽  
The United States ◽  
Fitness Cost ◽  
Eastern United States ◽  
Host Genotype ◽  
Podosphaera Macularis

Host resistance, both quantitative and qualitative, is the preferred long-term approach for disease management in many pathosystems, including powdery mildew of hop (Podosphaera macularis). In 2012, an epidemic of powdery mildew occurred in Washington and Idaho on previously resistant cultivars whose resistance was putatively based on the gene designated R6. In 2013, isolates capable of causing severe disease on cultivars with R6-based resistance were confirmed in Oregon and became widespread during 2014. Surveys of commercial hop yards during 2012 to 2014 documented that powdery mildew is now widespread on cultivars possessing R6 resistance in Washington and Oregon, and the incidence of disease is progressively increasing. Pathogenic fitness, race, and mating type of R6-virulent isolates were compared with isolates of P. macularis lacking R6 virulence. All isolates were positive for the mating type idiomorph MAT1-1 and were able to overcome resistance genes Rb, R3, and R5 but not R1 or R2. In addition, R6-virulent isolates were shown to infect differential cultivars reported to possess the R6 gene and also the R4 gene, although R4 has not yet been broadly deployed in the United States. R6-virulent isolates were not detected from the eastern United States during 2012 to 2015. In growth chamber studies, R6-virulent isolates of P. macularis had a significantly longer latent period and produced fewer lesions on plants with R6 as compared with plants lacking R6, indicating a fitness cost to the fungus. R6-virulent isolates also produced fewer conidia when compared with isolates lacking R6 virulence, independent of whether the isolates were grown on a plant with or without R6. Thus, it is possible that the fitness cost of R6 virulence occurs regardless of host genotype. In field studies, powdery mildew was suppressed by at least 50% on plants possessing R6 as compared with those without R6 when coinoculated with R6-virulent and avirulent isolates. R6 virulence in P. macularis appears to be race specific and, at this time, imposes a measurable fitness penalty on the fungus. Resistance genes R1 and R2 appear to remain effective against R6-virulent isolates of P. macularis in the U.S. Pacific Northwest.

scholarly journals First Report of A1 Mating Type of Phytophthora ramorum in North America

Plant Disease ◽  
2003 ◽  
Vol 87 (10) ◽  
pp. 1267-1267 ◽  
Author(s):  
E. M. Hansen ◽  
P. W. Reeser ◽  
W. Sutton ◽  
L. M. Winton ◽  
N. Osterbauer
Keyword(s):  
United States ◽  
Mating Type ◽  
North American ◽  
The United States ◽  
Phytophthora Ramorum ◽  
United States Department ◽  
Jackson County ◽  
Nursery Stock ◽  
Clackamas County ◽  
Microsatellite Alleles

Phytophthora ramorum is known in Europe and the western United States (1). In Europe, it is found in nurseries and landscape plantings. In the United States, it has been confined to coastal forests, and in California, it is found in a few horticultural nurseries. All European isolates tested have been A1 mating type, while all North American isolates were A2 mating type (2). Amplified fragment length polymorphism markers also indicated that the populations on the two continents are distinct, and nearly all North American isolates are from one clone (Kelly Ivors, unpublished). In June 2003, P. ramorum was isolated from diseased Viburnum and Pieris spp. cultivars from a Clackamas County nursery in northern Oregon and diseased Camellia sp. cultivar from a Jackson County nursery in southern Oregon. Representative isolates were submitted to the American Type Culture Collection, Manassas, VA. As part of the effort to determine the origin of these new infestations, we tested the nursery isolates for mating type. Seven Oregon nursery isolates, three Oregon forest isolates (from the predominant North American clone), and two European isolates were paired. Agar plugs from 3-day-old colonies were placed in close proximity on carrot agar plates, and then the plates were examined for oogonia after 3 and 10 days as advised by C. M. Brasier (personal communication). Oogonia and antheridia typical of P. ramorum (2) formed when isolates from the Clackamas County nursery were paired with the Oregon forest isolates and also when isolates from the Jackson County nursery were paired with the European isolates. Gametangia also formed in pairings between Oregon forest isolates and European isolates, but not in any other combinations. We developed polymerase chain reaction (PCR) primers for four microsatellite loci and determined allele sizes for the same set of isolates (unpublished). Microsatellite alleles of the Clackamas County isolates were identical to the European tester isolates, and alleles of the Jackson County isolates were identical to the Oregon forest isolates. These results indicate that the recent Oregon nursery infestations are of separate origins. The Clackamas County isolates are A1 mating type and have microsatellite alleles like the European testers, but according to shipping records, the nursery has received no host nursery stock directly from Europe. However, host nursery stock has been received from a Canadian nursery. The Jackson County isolates are of A2 mating type and have microsatellite alleles like the forest isolates of Oregon, which is consistent with the reported origin of these plants from a California nursery. These preliminary microsatellite results need to be validated against a larger isolate set but are congruent with the mating type results. The Oregon nursery infestations highlight the dangers of unregulated or underregulated transport of host nursery stock from infested areas to noninfested areas. All host plants from infested nursery blocks at the affected Oregon nurseries have been destroyed by incineration, and a monitoring program has been implemented. Other host nursery stock on site has been taken “off-sale” pending verification that it is disease free, per the United States Department of Agriculture, APHIS requirements. References: (1) J. M. Davidson et al. On-line publication. doi:10.1094/PHP-2003-0707-01-DG. Plant Health Progress, 2003. (2) S. Werres et al. Mycol. Res. 105:1155, 2001.

scholarly journals First Detection of Phytophthora ramorum Mating Type A2 in Europe

Plant Disease ◽  
2003 ◽  
Vol 87 (10) ◽  
pp. 1266-1266 ◽  
Author(s):  
Sabine Werres ◽  
Daphné De Merlier
Keyword(s):  
United States ◽  
The Netherlands ◽  
Mating Type ◽  
The United States ◽  
Phytophthora Species ◽  
Phytophthora Ramorum ◽  
Taxus Baccata ◽  
Camellia Japonica ◽  
Wide Range

Since its original isolation in 1993, Phytophthora ramorum has become an important pathogen. Initially, it was determined to be the causal agent of a twig blight of Rhododendron spp. in Germany and the Netherlands (3). Around the same period, symptoms and mortality on oak (Quercus spp.) and tanoak (Lithocarpus densiflorus) were associated with P. ramorum in California (2), where the disease was named sudden oak death. Subsequently, P. ramorum has been detected on a wide range of forest trees and shrub species in the United States. In Europe, the pathogen has spread to many countries, primarily on nursery plants of Rhododendron and Viburnum spp., and recently, on Camellia japonica, Kalmia latifolia, Pieris formosa var. forrestii, P. japonica, Leucothoe sp., Syringa vulgaris, and Taxus baccata. P. ramorum has not been observed in European forests. P. ramorum is heterothallic, and initial in vitro mating studies on agar media suggested that only the A1 mating type occurred in Europe, while only the A2 mating type was present in the United States (4). However, an isolate collected in 2002 in Belgium (1) appears to be the A2 mating type. This isolate (CBS 110901, Centraal Bureau voor Schimmelcultures, Baarn, the Netherlands) originated from an imported V. bodnantense plant at an ornamental nursery. A hyphal tip culture (BBA 26/02) of this isolate produced no oogonia on carrot piece agar after 6 weeks in pairing tests with other Phytophthora species of mating type A2. When paired with mating type A1 of P. cambivora, P. cinnamomi, P. cryptogea, and P. drechsleri, however, oogonia were observed in all pairings within 6 weeks. The number of oogonia was low in all pairings but was highest in pairings with P. cryptogea. No oospores were produced after 6 weeks between P. ramorum isolates BBA 26/02 and BBA 9/95 (from the holotype, mating type A1), but gametangia were observed when these isolates were paired on Rhododendron sp. twigs. Normal oogonia were produced on the outgrowing mycelium when pieces from these twigs were placed on carrot piece agar. The shape and size of the oogonia produced on carrot piece agar after pairing with P. cryptogea and on Rhododendron sp. twigs after pairing with P. ramorum BBA 9/95 were similar (24 to 34 μm, mean 29.6 μm and 25 to 33 μm, mean 30.6 μm, respectively). To our knowledge, this is the first observation of P. ramorum mating type A2 in Europe. References: (1) D. De Merlier et al. Plant Dis. 87:203, 2003. (2) D. M. Rizzo et al. Plant Dis. 86:205, 2002. (3) S. Werres et al. Mycol. Res. 105:1166, 2001. (4) S. Werres and B. Zielke. J. Plant Dis. Prot. 110:129, 2003.

System-wide seismic vulnerability of aging multiple-span multiple-girder bridges in low-to-moderate seismic hazard regions

2021 ◽  
Vol 37 (1_suppl) ◽  
pp. 1626-1651
Author(s):  
John E Lens M.EERI ◽  
Mandar M Dewoolkar ◽  
Eric M Hernandez M.EERI
Keyword(s):  
United States ◽  
Seismic Hazard ◽  
Cross Sections ◽  
Seismic Vulnerability ◽  
Time History ◽  
The United States ◽  
National Database ◽  
Eastern United States ◽  
Girder Bridges ◽  
The Status

This article describes the approach, methods, and findings of a quantitative analysis of the seismic vulnerability in low-to-moderate seismic hazard regions of the Central and Eastern United States for system-wide assessment of typical multiple span bridges built in the 1950s through the 1960s. There is no national database on the status of seismic vulnerability of bridges, and thus no means to estimate the system-wide damage and retrofit costs for bridges. The study involved 380 nonlinear analyses using actual time-history records matched to four representative low-to-medium hazard target spectra corresponding with peak ground accelerations from approximately 0.06 to 0.3 g. Ground motions were obtained from soft and stiff site seismic classification locations and applied to models of four typical multiple-girder with concrete bent bridges. Multiple-girder bridges are the largest single category, comprising 55% of all multiple span bridges in the United States. Aging and deterioration effects were accounted for using reduced cross-sections representing fully spalled conditions and compared with pristine condition results. The research results indicate that there is an overall low likelihood of significant seismic damage to these typical bridges in such regions, with the caveat that certain bridge features such as more extensive deterioration, large skews, and varied bent heights require bridge-specific analysis. The analysis also excludes potential damage resulting from liquefaction, flow-spreading, or abutment slumping due to weak foundation or abutment soils.

Force, Fraud, and Coercion—What Do They Mean? A Study of Victimization Experiences in a New Destination Latino Sex Trafficking Network

2016 ◽  
Vol 13 (5) ◽  
pp. 456-476 ◽  
Author(s):  
Lauren Copley Sabon
Keyword(s):  
United States ◽  
Law Enforcement ◽  
Social Constructionism ◽  
Sex Trafficking ◽  
The United States ◽  
Social Forces ◽  
Eastern United States ◽  
Latina Immigrants ◽  
Strict Interpretation ◽  
New Destination

In response to increasing Latino new destination migration in the United States, Latino sex trafficking networks have emerged in many of these areas. This article examines victimization experiences of Latina immigrants trafficked by a regional network operating in the Eastern United States drawn from law enforcement records and interviews with legal actors involved in the criminal case. The stories shared with law enforcement by the Latina victims gives insight into their lives, experiences in prostitution, and the operation of a trafficking/prostitution network (all lacking in the literature). Through the analytical frame of social constructionism, this research highlights how strict interpretation of force, fraud, coercion, and agency used to define “severe forms of trafficking” in the TVPA limits its ability to recognize many victimization experiences in trafficking situations at the hands of traffickers. The forms of coercion used in the criminal enterprise under study highlights the numerous ways it can be wielded (even without a physical presence) and its malleability as a concept despite legal definitional rigidity. The lack of legal recognition of the plurality of lived experiences in which agency and choice can be mitigated by social forces, structural violence, intersectional vulnerabilities, and the actions of others contributes to the scholarly critique of issues prosecuting trafficking cases under the TVPA and its strict legal definitions.

Effects of temporal variations in seismicity on seismic hazard

1981 ◽  
Vol 71 (1) ◽  
pp. 321-334
Author(s):  
Robin K. McGuire ◽  
Theodore P. Barnhard
Keyword(s):  
United States ◽  
Seismic Hazard ◽  
Seismic Activity ◽  
Time Window ◽  
Hazard Analysis ◽  
The United States ◽  
Temporal Variations ◽  
Seismogenic Zone ◽  
Eastern United States ◽  
Ground Shaking

abstract The accuracy of stationary mathematical models of seismicity for calculating probabilities of damaging shaking is examined using the history of earthquakes in China from 1350 A.D. to 1949 A.D. During this time, rates of seismic activity varied periodically by a factor of 10. Probabilities of damaging shaking are calculated in 62 cities in North China using 50 yr of earthquake data to estimate seismicity parameters; the probabilities are compared to statistics of damaging shaking in the same cities for 50 yr following the data window. These comparisons indicate that the seismic hazard analysis is accurate if: (1) the maximum possible earthquake size in each seismogenic zone is determined from the entire seismic history rather than from a short-time window; and (2) the future seismic activity can be estimated accurately. The first condition emphasizes the importance of realistically estimating the maximum possible size of earthquakes on faults. The second indicates the need to understand possible trends in seismic activity where these exist, or to develop an earthquake prediction capability with which to estimate future activity. Without the capability of estimating future seismicity, stationary models provide less accurate but generally conservative indications of seismic ground-shaking hazard. In the United States, the available earthquake history is brief but gives no indication of changing rates of activity. The rate of seismic strain release in the Central and Eastern United States has been constant over the last 180 yr, and the geological record of earthquakes on the southern San Andreas Fault indicates no temporal trend for large shocks over the last 15 centuries. Both observations imply that seismic activity is either stationary or of such a long period that it may be treated as stationary for seismic hazard analyses in the United States.

The Origins of Western Dermatology

2010 ◽  
Vol 14 (6) ◽  
pp. 255-266
Author(s):  
J. Barrie Ross
Keyword(s):  
United States ◽  
Eighteenth Century ◽  
Middle Ages ◽  
The United States ◽  
Historical Background ◽  
Eastern United States ◽  
The West ◽  
Defining Moments ◽  
Late Eighteenth Century ◽  
Late Eighteenth

Objective: On the premise that historical background makes the present more understandable, this review covers the origins of Western dermatology from its Greek and Roman origins through the Middle Ages to the defining moments in the late eighteenth century. Background and Conclusion: The development of major European centers at this time became the background for future centers in the eastern United States in the midnineteenth century and, finally, to the West Coast of the United States and Canada by the midtwentieth century.

scholarly journals Long-term trends and drought: spatiotemporal variation in juvenile sex ratios of North American ducks

2021 ◽  
Author(s):  
Sage Ellis ◽  
Madeleine Lohman ◽  
James Sedinger ◽  
Perry Williams ◽  
Thomas Riecke
Keyword(s):  
United States ◽  
North American ◽  
Sex Ratios ◽  
Survival Rates ◽  
The United States ◽  
Avian Communities ◽  
Eastern United States ◽  
Bird Populations ◽  
Long Term Trends

Sex ratios affect population dynamics and individual fitness, and changing sex ratios can be indicative of shifts in sex-specific survival at different life stages. While climate- and landscape-change alter sex ratios of wild bird populations, long-term, landscape scale assessments of sex ratios are rare. Further, little work has been done to understand changes in sex ratios in avian communities. In this manuscript, we analyse long-term (1961-2015) data on five species of ducks across five broad climatic regions of the United States to estimate the effects of drought and long-term trends on the proportion of juvenile females captured at banding. As waterfowl have a 1:1 sex ratio at hatch, we interpret changes in sex ratios of captured juveniles as changes in sex-specific survival rates during early life. Seven of twelve species-region pairs exhibited evidence for long-term trends in the proportion of juvenile females at banding. The proportion of juvenile females at banding increased for duck populations in the western United States and typically declined for duck populations in the eastern United States. We only observed evidence for an effect of drought in two of the twelve species-region pairs, where the proportion of females declined during drought. As changes to North American landscapes and climate continue and intensify, we expect continued changes in sex-specific juvenile survival rates. More broadly, we encourage further research examining the mechanisms underlying long-term trends in juvenile sex ratios in avian communities.

scholarly journals A Historical and Contemporary Discussion of the Tourism Industry in the Appalachian Region of the United States, with an analysis on its economic and sociological effects

Perceptions ◽  
2018 ◽  
Vol 4 (1) ◽  
Author(s):  
Nick Tarpey
Keyword(s):  
United States ◽  
The United States ◽  
Tourism Industry ◽  
Service Industries ◽  
Ethnic Composition ◽  
Easy Access ◽  
Eastern United States ◽  
Appalachian Region ◽  
Unique Region ◽  
Appalachian Regional Commission

Appalachia is defined as a roughly 1,000-mile long region in the eastern United States nestled in and around the Appalachian mountains. It is roughly 205,000 square miles and contains all or parts of twelve states: Alabama, Georgia, Mississippi, Kentucky, Maryland, New York, Pennsylvania, Virginia, North Carolina, Tennessee, West Virginia, and Ohio. The area was  home to about 25 million people as of the 2010 census. It is important to note that the region has struggled with outmigration since the 1930s beginning with the onset of the Great Depression. (Appalachian Regional Commission 2017). Historically, Appalachia has been known as a unique region in the United States. Beginning with roots as a common settlement region for fiery Scotch-Irish immigrants in the 1700s, continued by earning a reputation as a center for moonshine production during the 1930s, and now known as a region where the wealthy buy their second and third homes, the region has consistently been able to craft its own, particular culture. With a population that is 42% rural (compared to a 20% rural population for the entire U.S.) and overwhelmingly Scotch-Irish in ethnic composition, the area differs from the mainstream US. Beset by poverty, the region needs tourism to be a viable industry in many of its locales. A population that is relatively low in educational achievement (Appalachia as whole averages a 22% college completion rate per county compared with a US rate of 29% per county) and does not have easy access to intellectual resources in many places needs a stable, job-providing industry (Appalachian Regional Commission 2017). The area once had a legacy in the mining and forestry industries, but according to the Appalachian Regional Commission, that era has passed and people now rely on a rebirth of manufacturing, service industries, and tourism to provide jobs (2017). Fortunately, the situation in Appalachia has improved since 1960, as the number of economically distressed counties in the region has declined from 295 in 1960 to 91 in 2014 (Appalachian Regional Commission 2017). The poverty rate of 17.1% is slightly above the national average of 14.3% (Appalachian Regional Commission 2017). The region has come to increasingly depend on the tourism industry to fill an economic void as gaps in basic services and the continual draining of potential intellectual capital from population loss continue to plague the area. This paper will examine contemporary perspectives  on tourism in the Appalachian region and analyze their economic and sociological effects.

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