First Report of Red Crown Rot Caused by Cylindrocladium parasiticum on Soybean in Guangdong, Southern China

In October 2006, occurrence of a disease on soybean (Glycine max) was observed in Guangdong Province of southern China with symptoms resembling red crown rot (3,4). Reddish brown lesions girdled the basal stems with numerous reddish orange perithecia on the lesion surface. Roots became black and rotted and whole plants wilted and died. More recently, outbreaks of this disease were observed in several counties in Guangdong. Disease incidence reached as much as 80% on cv. Huaxia 3 in some fields, causing severe yield losses. Isolation was made from the edge of lesions on potato dextrose agar at 25°C. The fungus produced white, aerial mycelia and a burnt orange-to-dark brown submerged growth. Conidiophores were borne laterally on a stipe, terminating in a hyaline, globose vesicle measuring 4.0 to 13.0 μm in diameter. After branching from the stipe, the conidiophore continued to develop by forming two to three single-celled branches. These cells might give rise to two or three shorter branches (14.8 to 36.9 μm long) and phialides. The phialides were doliform, nonseptate, and measured 7.4 to 19.7 × 3.7 to 4.9 μm. Conidia were hyaline, cylindrical with one to three septa (mostly three septa), and measured 54.1 to 76.3 × 4.9 to 7.4 μm. Perithecia were orange to red, subglobose to oval or globose, 212.1 to 454.5 μm high, and 111.1 to 333.3 μm wide. Asci were hyaline, clavate, thin walled, long stalked, measured 121.0 to 200.8 × 11.5 to 25.6 μm, and each contained eight ascospores that became aggregated in the upper half of the ascus at maturity. The ascospores were hyaline, fusoid to falcate with one to three septa (mostly with one septum), constricted slightly at the septum, and measured 29.5 to 73.8 × 4.9 to 9.8 μm. The fungus was identified as Cylindrocladium parasiticum (teleomorph Calonectria ilicicola) (1,2). The beta-tubulin gene fragment sequences of three isolates were obtained (one sequence being GenBank Accession No. GU073284) and comparisons with GenBank showed 99 to 100% similarity with Calonectria ilicicola (EF159730 and AY725643). Pathogenicity was confirmed by inoculation of 2-week-old seedlings of cv. Huaxia 3 in plastic pots (10 × 9 cm) by drenching with a conidia suspension (105 conidia per ml). All inoculated plants showed similar red crown rot symptoms on stem bases and roots 1 week after inoculation. C. parasiticum was reisolated from the diseased plants, and many orange-to-red perithecia of Calonectria ilicicola were formed on the lesions 3 weeks after inoculation. This pathogen may pose a serious threat to >300,000 ha of soybean production as well as >300,000 ha of peanut production in Guangdong Province. It has been previously reported in Jiangsu Province in eastern China (3) and Yunnan Province in western China (4). References: (1) D. K. Bell, and E. K. Sobers. Phytopathology 56:1361, 1966. (2) P. W. Crous et al. Mycol. Res. 97:889, 1993. (3) J. Y. Gai et al. Soybean Sci. (in Chinese) 11:113, 1992. (4) Z. H. Ma et al. Plant Pathol. 53:537, 2004.

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First Report of Peanut Cylindrocladium Black Rot Caused by Cylindrocladium parasiticum in Fujian Province, Eastern China

Plant Disease ◽

10.1094/pdis-11-11-0982 ◽

2012 ◽

Vol 96 (4) ◽

pp. 583-583 ◽

Cited By ~ 4

Author(s):

R. Pan ◽

Q. Deng ◽

D. Xu ◽

C. Ji ◽

M. Deng ◽

...

Keyword(s):

Southern China ◽

Disease Incidence ◽

Eastern China ◽

Black Rot ◽

Brown Pigment ◽

Fujian Province ◽

First Report ◽

Calonectria Ilicicola ◽

Cylindrocladium Parasiticum ◽

Wheat Kernels

During late July and early August of 2010, a serious disease of peanut (Arachis hypogaea L.) resembling Cylindrocladium black rot (CBR) was found in Longyan City, Fujian Province of Eastern China. Aboveground symptoms were chlorosis and yellowing of leaves, a black rot of the basal stem and pegs, and wilting of the vines. Underground plant parts (including pods, pegs, taproots, and hypocotyls) were blackened and rotted. Orange-to-reddish spherical fruiting bodies appeared on the lesions of the basal stems and pegs of peanut. Disease incidence was approximately 20%. A fungus was consistently isolated from the edge of lesions on potato dextrose agar (PDA) amended with streptomycin and incubated at 25°C. The fungus produced white-to-pale buff mycelia with a yellowish brown pigment. Optimum growth of the fungus on PDA was at 25 to 30°C. Conidiophores were borne laterally on a stipe that terminated in a hyaline, globose vesicle measuring 5.5 × 10.9 μm in diameter. Conidia were hyaline, cylindrical, rounded at both ends, slightly wider at the base than at the apex, with one to three septa (mostly one septa), and measured 27.3 to 70.9 × 4.1 to 8.2 μm. Orange-to-reddish perithecia were readily formed in old cultures. The perithecia were subglobose to oval or obovate and measured 215.6 to 609.4 × 309.4 to 496.9 μm. The asci were hyaline, clavate, thin walled, long stalked, with each containing eight ascospores. Ascospores were hyaline, falcate, had one septum, and measured 27.3 to 54.5 × 4.1 to 6.8 μm. The fungus was identified as Cylindrocladium parasiticum Crous, M.J. Wingfield, & Alfenas (teleomorph Calonectria ilicicola) (1,2). The beta-tubulin gene fragment was amplified using the T1/Bt2b primers (3) and sequenced. The sequences of three isolates (GenBank Accession Nos. JF343965, JF429656, and JF429657), when compared with existing sequences in GenBank, had 95 to 99% sequence identity with Calonectria ilicicola (GenBank Accession Nos. AY725643 and AY725639). Pathogenicity tests were conducted by first culturing the fungus on wheat kernels for 2 weeks. Inoculated kernels were then used as inoculum and mixed with sterilized soil in a proportion of 1:20 by weight in plastic pots (10 × 9 cm). Noninoculated wheat kernels were mixed with sterilized soil in the same proportion and served as the control. Two-week-old peanut seedlings (cv. Yueyou No. 7) were transplanted into inoculated or noninoculated pots. There were five plants per pot and each treatment was replicated four times. The plants were incubated in a greenhouse at 25 ± 2°C. All of the treated plants exhibited typical basal stem and root rot symptoms of CBR 2 weeks after inoculation, while all of the control plants remained healthy. C. parasiticum was reisolated from the diseased plants. To our knowledge, this is the first report of CBR on peanut in Fujian Province in Eastern China. The disease has been previously reported in Guangdong Province in Southern China but is not known elsewhere (4). This pathogen may pose a serious threat to peanut production in China, where peanut is an important crop. References: (1) D. K. Bell and E. K. Sobers. Phytopathology 56:1361, 1966. (2) P. W. Crous et al. Mycol. Res. 97:889, 1993. (3) P. W. Crous et al. Can. J. Bot. 77:1813, 1999. (4) R. Pan et al. Plant Pathol. 58:1176, 2009.

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First Report of Cylindrocladium Black Rot of Peanut Caused by Cylindrocladium parasiticum (Teleomorph Calonectria ilicicola) in Jiangxi Province, China

Plant Disease ◽

10.1094/pdis-11-11-1010 ◽

2012 ◽

Vol 96 (4) ◽

pp. 586-586 ◽

Cited By ~ 5

Author(s):

Y. Gai ◽

Q. Deng ◽

R. Pan ◽

X. Chen ◽

M. Deng

Keyword(s):

Southern China ◽

Disease Incidence ◽

Jiangxi Province ◽

Black Rot ◽

Brown Pigment ◽

Fruiting Bodies ◽

Calonectria Ilicicola ◽

Cylindrocladium Parasiticum ◽

Stem Lesions ◽

Reddish Orange

In July 2010, a serious disease of peanut (Arachis hypogaea) resembling Cylindrocladium black rot (CBR) was found in Longnan County, Jiangxi Province, China. Symptoms included chlorotic, yellowish and blighted leaves, and wilting of the plants. Taproots and hypocotyls were blackened and rotted. Clusters of reddish orange spherical fruiting bodies appeared in the lesions present on basal stems, pegs, pods, and roots of peanut. Disease incidence reached as much as 50% in some patches of the field. Plants with symptoms were sampled from fields. Microscopic examination revealed that the reddish orange, spherical fruiting bodies were the perithecia and measured 461.6 (337.5 to 609.4) × 395.5 (309.4 to 496.9) μm. With gentle pressure, asci and ascospores were exuded from perithecia. The asci were hyaline, thin walled, and long stalked. Ascospores were hyaline, falcate with one septum, and measured 43.5 (27.3 to 54.5) × 5.6 (4.1 to 6.8) μm with a length/width (L/W) ratio of 7.8 ± 1.3. A fungus with white-to-pale buff border mycelia and yellowish brown pigment was consistently isolated from the edge of basal stem lesions on potato dextrose agar at 25°C. Mycelia grew at temperatures ranging from 8 to 32°C and the optimum was 25 to 26°C. To determine the species, single-conidial isolates of the fungus were cultured on carnation leaf agar for 7 days at 25°C and 12 h of light/dark conditions. Conidia were hyaline, cylindrical with one to three septa (mostly three septa), and measured 49.3 (27.3 to 70.9) × 5.9 (4.1 to 6.8) μm with L/W ratio of 8.4 ± 1.6. Vesicles were globose and measured 5.5 to 10.9 μm in diameter. The fungus was identified as Cylindrocladium parasiticum (teleomorph Calonectria ilicicola) (1,2). A PCR assay was conducted on one representative isolate (JXLN32) by analyzing multilocus sequences of the TUB2 (coding β-tubulin protein), ACT (coding actin), and CaM gene (coding calmodulin protein) and were amplified and sequenced using the primers reported by Crous et al. (3). Sequences of the studied DNA regions were submitted to GenBank (Accession Nos. TUB2: JF429649; ACT: JQ070809; and CaM: JQ070808). BLAST searches with the existing sequences in GenBank showed that there was 99 to 100% identity with the existing sequences of C. ilicicola (GenBank Accession Nos. TUB2: AY725643; ACT: GQ280446; and CaM: GQ267402). To complete Koch's postulates, inoculum was prepared by mixing the microsclerotia (MS) suspension of the isolate (JXLN32) with soil at a proportion of 10 MS per g of soil. Ten replicate plastic pots containing five peanut seeds (cv. Yueyou 7) each were planted and placed in a glasshouse at 25 ± 2°C. The same number of peanut seeds was used as an uninoculated control. Typical basal stem and roots rot symptoms of CBR were observed in 2 months and C. parasiticum was reisolated from these inoculated diseased plants. No symptoms were detected on the control plants. To our knowledge, this is the first finding of Cylindrocladium black rot in Jiangxi Province, which is the main peanut-producing area in China. The disease has been previously reported in Guangdong Province in southern China but is not known elsewhere (4). Because of its ability to spread through seed and soil and its destructive potential, this pathogen may pose a serious threat to peanut production in China. References: (1) D. K. Bell and E. K. Sobers. Phytopathology 56:1361, 1966. (2) P. W. Crous et al. Mycol. Res. 97:889, 1993. (3) P. W. Crous et al. Stud. Mycol. 50:415, 2004. (4) R. Pan et al. Plant Pathol. 58:1176, 2009.

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Phylogenetic diversity of Calonectria ilicicola causing Cylindrocladium black rot of peanut and red crown rot of soybean in southern China

Journal of General Plant Pathology ◽

10.1007/s10327-017-0724-9 ◽

2017 ◽

Vol 83 (5) ◽

pp. 273-282 ◽

Cited By ~ 4

Author(s):

Yunpeng Gai ◽

Qiaowen Deng ◽

Xinglong Chen ◽

Mingfang Guan ◽

Xiang Xiao ◽

...

Keyword(s):

Phylogenetic Diversity ◽

Southern China ◽

Crown Rot ◽

Black Rot ◽

Calonectria Ilicicola ◽

Red Crown Rot

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First Report of Red Crown Rot of Soybeans Caused by Calonectria ilicicola (Anamorph: Cylindrocladium parasiticum) in Illinois

Plant Disease ◽

10.1094/pdis-01-19-0105-pdn ◽

2019 ◽

Vol 103 (7) ◽

pp. 1777-1777

Author(s):

N. Kleczewski ◽

D. Plewa ◽

C. Kangas ◽

E. Phillippi ◽

V. Kleczewski

Keyword(s):

Crown Rot ◽

First Report ◽

Calonectria Ilicicola ◽

Cylindrocladium Parasiticum ◽

Red Crown Rot

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Occurrence and source apportionment of perfluoroalkyl acids (PFAAs) in the atmosphere in China

Atmospheric Chemistry and Physics ◽

10.5194/acp-19-14107-2019 ◽

2019 ◽

Vol 19 (22) ◽

pp. 14107-14117 ◽

Cited By ~ 3

Author(s):

Deming Han ◽

Yingge Ma ◽

Cheng Huang ◽

Xufeng Zhang ◽

Hao Xu ◽

...

Keyword(s):

Degradation Products ◽

Southern China ◽

Eastern China ◽

Northern China ◽

Central China ◽

Western China ◽

Perfluoroalkyl Acids ◽

Average Value ◽

Pmf Model ◽

Air Sampler

Abstract. Perfluoroalkyl acids (PFAAs) are a form of toxic pollutant that can be transported across the globe and accumulated in the bodies of wildlife and humans. A nationwide geographical investigation considering atmospheric PFAAs via a passive air sampler (PAS) based on XAD (a styrene–divinylbenzene copolymer) was conducted in 23 different provinces/municipalities/autonomous regions in China, which provides an excellent chance to investigate their occurrences, spatial trends, and potential sources. The total atmospheric concentrations of 13 PFAAs (n=268) were 6.19–292.57 pg m−3, with an average value of 39.84±28.08 pg m−3, which were higher than other urban levels but lower than point source measurements. Perfluorooctanoic acid (PFOA) was the dominant PFAA (20.6 %), followed by perfluorohexanoic acid (PFHxA), perfluorooctane sulfonate (PFOS), and perfluoroheptanoic acid (PFPeA). An increasing seasonal trend of PFAA concentrations was shown as summer < autumn < spring < winter, which may be initiated by stagnant meteorological conditions. Spatially, the content of PFAAs displayed a declining gradient trend of central China > northern China > eastern China > north-eastern China > south-western China > north-western China > southern China, and Henan contributed the largest proportion of PFAAs. Four sources of PFAAs were identified using a positive matrix factorization (PMF) model, including PFOS-based products (26.1 %), products based on PFOA and perfluorononanoic acid (PFNA; 36.6 %), degradation products of fluorotelomer-based products (15.5 %), and an unknown source (21.8 %).

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Patterns and trends of high-impact weather in China during 1959–2014

Natural Hazards and Earth System Science ◽

10.5194/nhess-16-855-2016 ◽

2016 ◽

Vol 16 (3) ◽

pp. 855-869 ◽

Cited By ~ 9

Author(s):

Jun Shi ◽

Kangmin Wen ◽

Linli Cui

Keyword(s):

Southern China ◽

Eastern China ◽

High Impact ◽

Northeastern China ◽

Central China ◽

Western China ◽

Tibet Plateau ◽

High Mountain ◽

Mitigation And Adaptation ◽

High Impact Weather

Abstract. The spatial and temporal characteristics of the frequencies of four types of high-impact weather (HIW), i.e. snowfall, thunderstorms, fog and hailstorms, were analysed in China during 1959–2014. Results indicate a significant decrease in the number of snowfall days, thunderstorm days and thunderstorm spells in all six regions of China, with regional decreasing rates of 0.1–3.4 days, 1.6–5.1 days and 0.23–0.77 times per decade respectively. The number of foggy days, hailstorm days and snowfall spells decreased at rates of 0.2–1.8 days, 0.1–0.7 days and 0.14–0.44 times per decade respectively in almost all regions and fog and hailstorm spells decreased at rates of 0.06–0.17 and 0.001–0.043 times per decade respectively in most regions of China. Spatially, there was more snowfall in northeastern China and western China, and more thunderstorms in southern China and southwestern China. The number of fog events was larger in some high mountain stations, eastern China and central China. Hailstorms were concentrated on Qinghai–Tibet Plateau. Over the past 56 years, snowfall days, thunderstorm days and thunderstorm spells decreased in most parts of China, and hailstorm days decreased in northeastern China, most parts of northern China and Tibet, southern Qinghai and western Sichuan. The spatial trends of foggy days, foggy spells, snowfall spells and hailstorm spells were not significant in most parts of China. With global warming, some types of HIW are likely to increase in their intensities, so more mitigation and adaptation strategies are still essential for local government and the public in China.

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First Report of Soybean Red Crown Rot Caused by Calonectria ilicicola in Taiwan

Plant Disease ◽

10.1094/pdis-06-19-1164-pdn ◽

2020 ◽

Vol 104 (3) ◽

pp. 979

Author(s):

H. H. Liu ◽

Y. M. Shen ◽

H. X. Chang ◽

M. N. Tseng ◽

Y. H. Lin

Keyword(s):

Crown Rot ◽

First Report ◽

Calonectria Ilicicola ◽

Red Crown Rot

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A 5-yr Climatology of Severe Convective Wind Events over China

Weather and Forecasting ◽

10.1175/waf-d-16-0101.1 ◽

2017 ◽

Vol 32 (4) ◽

pp. 1289-1299 ◽

Cited By ~ 7

Author(s):

Xinlin Yang ◽

Jianhua Sun ◽

Yongguang Zheng

Keyword(s):

High Frequency ◽

North China ◽

Eastern China ◽

Warm Season ◽

Guangdong Province ◽

Bimodal Distribution ◽

Western China ◽

Seasonal And Diurnal Variations ◽

Report Data ◽

Wind Events

Abstract A method using cloud-to-ground lightning was developed to retrieve severe convective wind (SCW) events from significant weather report data over China during the period 2010–14. The results showed that SCW events were a feature of local weather activity, and their distribution showed clear seasonal and diurnal variations. The SCW events mainly occurred over eastern China during the midafternoon in the warm season and rarely occurred over western China. The highest frequency of SCW events was recorded in north China and Guangdong Province. There was also a high frequency of SCW events in the middle and lower reaches of the Yangtze River. The most frequent occurrence of SCW events was in Guangdong Province in spring, while a high frequency of SCW events was observed in both north China and Guangdong Province during the summer months. The peak month for SCW events was July over the whole of China and June in north China. The pattern in Guangdong Province had a bimodal distribution, with the peak months being May and August. The majority of SCW events occurred between 1200 and 2000 local time.

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A Functional Variant of CXCL16 Predisposes to Sepsis and MODS in Traumatic Patients

10.21203/rs.3.rs-71609/v1 ◽

2020 ◽

Author(s):

Jianhui Sun ◽

Huacai Zhang ◽

Di Liu ◽

Li Cui ◽

Qiang Wang ◽

...

Keyword(s):

Major Trauma ◽

Southern China ◽

Eastern China ◽

Guizhou Province ◽

Western China ◽

Morbidity Rate ◽

Trauma Patients ◽

Chinese Han ◽

Increased Risk

Abstract Background: CXC chemokine famliy genes play an important role in inflammatory and immune diseases. CXCL16 affects the occurrence and development of inflammation through leukocyte chemotaxis, leukocyte adhesion and endotoxin clearance. We selected a set of tagSNPs in CXCL16 gene and investigated their clinical relevance in relation to the development of sepsis and MODS in patients with major trauma in three independent Chinese Han populations.Methods: A total of 1,620 major trauma patients were enrolled in this study. Among which, 920 patients came from Chongqing in western China, 350 patients came from Zhejiang province in eastern China and 350 patients came from Guizhou province in southern China. Improved multiplex ligation detection reaction (iMLDR) was used in the genotyping and genetic association study was used to analyze the association between CXCL16 haplotypes and sepsis morbidity rate and higher MOD Scores in three cohorts. Results: CXCL16 T123V181 haplotype was associated with an increased risk for sepsis morbidity rate and higher MOD Scores in three cohorts. In vitro chemotactic experiment showed that T123V181 protein enhanced the chemotaxis of immunocytes. The adhesion ability of THP-1 cells which expressing T123V181 to immunocytes was also stronger than that of the other three haplotypes. T123 and V181 altered the structure of CXCL16 active center, which led to the change of protein function and the change of adhesion and chemotaxis of CXCL16 expressing immunocytes. The structural change might be the cause of the increased incidence of sepsis and higher score of MODS.Conclusions: We demonstrate that CXCL16 genetic variation regulates sepsis morbidity rate and explore a paradigm for the prewarning diagnosis of sepsis tailored by individual genetic information.Trial registration： ClinicalTrials.gov , NCT01713205. Registered 18 October 2012, retrospectively registered.

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