scholarly journals First Report of a Cucumber mosaic virus-Associated Satellite RNA in Lobelia erinus

Plant Disease ◽  
2001 ◽  
Vol 85 (7) ◽  
pp. 802-802 ◽  
Author(s):  
S. G. P. Nameth ◽  
J. R. Fisher
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Leaf Tissue ◽  
Late Summer ◽  
Culture Collection ◽  
Northern Hybridization ◽  
Enzyme Linked Immunosorbent Assay ◽  
Satellite Rna ◽  
First Report ◽  
Dna Labeling

Lobelia (Lobelia erinus L.) is a common herbaceous annual used in flower beds and hanging baskets. The plant blooms from early to late summer. In the summer of 2000, Lobelia plants expressing virus-like symptoms were collected from a greenhouse-based production site in Ohio. Affected plants expressed a mild leaf mosaic and stunting. Viral-associated dsRNA was isolated from 7 g of symptomatic leaf tissue (1). Four dsRNAs were observed at 3.9, 3.0, 2.25, and 1.05 kb indicating the presence of a Cucumovirus. A fifth dsRNA at 0.75 kb also was observed, consistent with the presence of a satellite RNA. Enzyme-linked immunosorbent assay (ELISA) analysis (Agdia, Inc., Elkhart, IN) of symptomatic Lobelia tissue confirmed the presence of Cucumber mosaic virus (CMV). A (S)CARNA-5 (-) cDNA clone (American Type Culture Collection #45124) was labeled with digoxygenin (DIG) as per the manufacturer's instructions (Genius II DIG-DNA Labeling Kit, Boehringer Mannheim) and used as a diagnostic probe to detect this satellite RNA. Northern hybridization confirmed the identity of the satellite RNA (2). This is the first report of any satellite RNA associated with a virus infection in Lobelia and the first report of CMV in this host in Ohio. References: (1) J. R. Fisher and S. G. P. Nameth. HortScience 35:230–234, 2000. (2) R. A. Valverde et.al. Plant Dis. 74:255–258, 1990.

scholarly journals First Report of Cucumber mosaic virus Subgroup II Infecting Lycopersicon esculentum in India

Plant Disease ◽  
2006 ◽  
Vol 90 (11) ◽  
pp. 1457-1457 ◽  
Author(s):  
N. Sudhakar ◽  
D. Nagendra-Prasad ◽  
N. Mohan ◽  
K. Murugesan
Keyword(s):  
Coat Protein ◽  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Tamil Nadu ◽  
Indian Subcontinent ◽  
Enzyme Linked Immunosorbent Assay ◽  
Infected Leaf ◽  
Polymorphism Analysis ◽  
First Report ◽  
Subgroup Ii

During a survey in January 2006 near Salem in Tamil Nadu (south India), Cucumber mosaic virus was observed infecting tomatoes with an incidence of more than 70%. Plants exhibiting severe mosaic, leaf puckering, and stunted growth were collected, and the virus was identified using diagnostic hosts, evaluation of physical properties of the virus, compound enzyme-linked immunosorbent assay (ELISA) (ELISA Lab, Washington State University, Prosser), reverse-transcription polymerase chain reaction (RT-PCR), and restriction fragment length polymorphism analysis (DSMZ, S. Winter, Germany). To determine the specific CMV subgroup, total RNA was extracted from 50 infected leaf samples using the RNeasy plant RNA isolation kit (Qiagen, Hilden, Germany) and tested for the presence of the complete CMV coat protein gene using specific primers as described by Rizos et al. (1). A fragment of the coat protein was amplified and subsequently digested with MspI to reveal a pattern of two fragments (336 and 538 bp), indicating CMV subgroup II. No evidence of mixed infection with CMV subgroup I was obtained when CMV isolates representing subgroups I (PV-0419) and II (PV-0420), available at the DSMZ Plant Virus Collection, were used as controls. Only CMV subgroup I has been found to predominantly infect tomato in the Indian subcontinent, although Verma et al. (2) identified CMV subgroup II infecting Pelargonium spp., an ornamental plant. To our knowledge, this is the first report of CMV subgroup II infecting tomato crops in India. References: (1) H. Rizos et al. J. Gen. Virol. 73:2099, 1992. (2) N. Verma et al. J. Biol. Sci. 31:47, 2006.

scholarly journals First Report of Cucumber Mosaic Virus in Eryngium amethystinum, Canna spp., and Aquilegia hybrids in Ohio

Plant Disease ◽  
1997 ◽  
Vol 81 (11) ◽  
pp. 1331-1331 ◽  
Author(s):  
J. R. Fisher ◽  
M.-C. Sanchez-Cuevas ◽  
S. T. Nameth ◽  
V. L. Woods ◽  
C. W. Ellett
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Plant Viruses ◽  
Early Summer ◽  
Reservoir Host ◽  
Enzyme Linked Immunosorbent Assay ◽  
Herbaceous Plant ◽  
First Report ◽  
Severe Stunting ◽  
Banding Profile

Eryngium amethystinum (amethyst sea holly) is a herbaceous plant commonly grown as an ornamental perennial in U.S.D.A. hardiness zones 3 to 8. The plant thrives in dry areas with infertile soils and the flowers are often used in dried floral arrangements. Canna spp. (Canna), soft perennials (U.S.D.A. zone 9 and above), are becoming popular flowering plants because of their bright flowers and spectacular foliage. There are a variety of species that fall under the heading Canna spp., of which the most popular are C. glauca, C. indica, C. edulis, and C. iridiflora. Hybrids of Aquilegia (garden columbine), a hardy perennial (U.S.D.A. zones 3 to 9), flower in late spring through early summer. The genus is made up of a wide variety of cultivars. E. amethystinum exhibiting severe mosaic, yellowing, and stunting, along with Canna plants exhibiting severe stunting, chlorotic and distorted foliage, and mosaic, and garden columbine plants exhibiting stunting, leaf curl, chlorosis, and mosaic, collected from commercial plantings throughout the central Ohio area, were analyzed for the presence of virus infection with viral-associated, double-stranded RNA (dsRNA) analysis. dsRNA analysis resulted in a banding profile typical of that seen with members of the cucumovirus family of plant viruses. Plants positive for cucumovurus-like dsRNA were tested with a direct antibody sandwich enzyme-linked immunosorbent assay (ELISA). ELISA results confirmed the presence of cucumber mosaic virus (CMV) in all symptomatic plants tested. No evidence of dsRNA or CMV was found in any of the asymptomatic plants tested. Because all of these hosts are common in the perennial garden, they could serve as a reservoir host of CMV for other plants in the garden. This is the first report of CMV in E. amethystinum, Canna spp., and Aquilegia hybrids in Ohio.

scholarly journals Multigenic System Controlling Viral Systemic Infection Determined by the Interactions Between Cucumber mosaic virus Genes and Quantitative Trait Loci of Soybean Cultivars

2011 ◽  
Vol 101 (5) ◽  
pp. 575-582 ◽  
Author(s):  
Shizen Ohnishi ◽  
Issei Echizenya ◽  
Eri Yoshimoto ◽  
Kim Boumin ◽  
Tsuyoshi Inukai ◽  
...  
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Quantitative Trait ◽  
Single Gene ◽  
Systemic Infection ◽  
Northern Hybridization ◽  
Enzyme Linked Immunosorbent Assay ◽  
Cdna Clones ◽  
Long Distance ◽  
Chimeric Rnas

Soybean ‘Harosoy’ is resistant to Cucumber mosaic virus soybean strain C (CMV-SC) and susceptible to CMV-S strain D (CMV-SD). Using enzyme-linked immunosorbent assay and Northern hybridization, we characterized the Harosoy resistance and found that CMV-SC did not spread systemically but was restricted to the inoculated leaves in Harosoy. Harosoy resistance was not controlled by either a dominant or recessive single gene. To dissect this system controlling long-distance movement of CMV in soybean, we constructed infectious cDNA clones of CMV-SC and CMV-SD. Using these constructs and the chimeric RNAs, we demonstrated that two viral components were required for systemic infection by the virus. The region including the entire 2b gene and the 5′ region of RNA3 (mainly the 5′ untranslated region) together were required. By quantitative trait locus (QTL) analysis using an F2 population and the F3 families derived from Harosoy and susceptible ‘Nemashirazu’, we also showed that at least three QTLs affected systemic infection of CMV in soybean. Our study on Harosoy resistance to CMV-SC revealed an interesting mechanism, in which multiple host and viral genes coordinately controlled viral systemic infection.

scholarly journals First report of cucumber mosaic virus subgroups I and II with satellite RNA infecting Nicotiana glauca in Mexico

2019 ◽  
Vol 101 (3) ◽  
pp. 801-801
Author(s):  
Candelario Ortega-Acosta ◽  
Daniel L. Ochoa-Martínez ◽  
Javier Hernández-Morales
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Satellite Rna ◽  
Nicotiana Glauca ◽  
First Report

scholarly journals Transgenic Tomato Plants Expressing Satellite RNA Are Tolerant to Some Strains of Cucumber Mosaic Virus

1994 ◽  
Vol 119 (3) ◽  
pp. 642-647 ◽  
Author(s):  
P.B. McGarvey ◽  
M.S. Montasser ◽  
J.M. Kaper
Keyword(s):  
Transgenic Plants ◽  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Unit Length ◽  
Transgenic Tomato ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Satellite Rna ◽  
Tomato Plants ◽  
Transgenic Tomato Plants

Transgenic tomato plants (Lycopersicon esculentum Mill.) expressing cucumber mosaic virus (CMV) satellite RNA fused to a gene for β-glucuronidase were produced using Agrobacterium-mediated transformation. The R1 progeny of self-crossed R0 plants were challenge-inoculated with virion or RNA preparations of CMV or tomato aspermy virus (TAV). The transgenic plants challenged with CMV-1 showed mild disease symptoms in the first 2 weeks postchallenge followed by a decrease in symptoms, resulting in little difference between the transgenic and uninfected control group by the fourth week. Enzyme-linked immunosorbent assay results showed about a 10-fold decrease in virus accumulation in the transgenic plants compared to controls. Tolerance was evident only in plants that contained the recombinant insert and produced mature unit-length satellite RNA after CMV infection. Plants challenged with TAV showed no significant tolerance to virus-induced symptoms.

scholarly journals Cucumber mosaic virus Diagnosed in Desert Rose in Florida

Plant Disease ◽  
2003 ◽  
Vol 87 (8) ◽  
pp. 1007-1007 ◽  
Author(s):  
C. A. Baker ◽  
D. Achor ◽  
S. Adkins
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Chenopodium Quinoa ◽  
Enzyme Linked Immunosorbent Assay ◽  
Satellite Rna ◽  
Cmv Infection ◽  
Southwest Florida ◽  
Foliar Symptoms ◽  
The Family ◽  
Dark Green

Desert rose (Adenium obesum (Forssk) Roem. & Schult.) is a member of the family Apocynaceae and characterized by fleshy leaves and stems and colorful flowers. This popular, exotic ornamental, originally from southeastern Africa, is propagated vegetatively and is a perennial in warm climates. Virus-like foliar symptoms, including a mosaic with dark green islands surrounding the veins and chlorosis on the leaf margins, were observed on desert rose samples from two southwest Florida nurseries in November 2002. Cucumber mosaic virus (CMV) was identified in symptomatic plants by serological testing for the presence of CMV coat protein with a commercially available ImmunoStrip test (Agdia, Elkhart, IN). A third sample expressing similar symptoms was observed in southeastern Florida in February 2003. The presence of CMV was confirmed by serological detection with a commercially available double-antibody sandwich enzyme-linked immunosorbent assay (Agdia). An agent was mechanically transmitted from the third sample to Chenopodium quinoa, resulting in the formation of chlorotic local lesions. Examination of inoculated C. quinoa leaves by double-stranded (ds) RNA analysis and electron microscopy (leaf dips) revealed the presence of a typical cucumovirus dsRNA profile and spherical virions ~28 nm in diameter, respectively, providing additional confirmation of a CMV infection. A possible satellite RNA of ~350 nucleotides was also observed by dsRNA analysis. To our knowledge, this represents the first report of CMV infection of desert rose.

scholarly journals Polygala myrtifolia as a New Natural Host of Cucumber mosaic virus

Plant Disease ◽  
2002 ◽  
Vol 86 (12) ◽  
pp. 1403-1403 ◽  
Author(s):  
M. Tessitori ◽  
A. Reina ◽  
V. Catara ◽  
G. Polizzi
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Invasive Plant ◽  
Leaf Tissue ◽  
Impatiens Necrotic Spot Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
New Host ◽  
Polyclonal Antisera ◽  
Polygala Myrtifolia ◽  
Das Elisa

Cucumber mosaic virus (CMV), Tomato spotted wilt virus (TSWV), and Impatiens necrotic spot virus (INSV) are among the most important viral pathogens of ornamental plants (1). Polygala myrtifolia L. (myrtle-leaf milkwort), originating from South Africa, and a member of the Polygalaceae, was recently introduced in Italy as a cultivated ornamental shrub for its fast and attractive free-flowering growth and drought-resistant characteristics. It can become an invasive plant and is now considered a serious problem in coastal areas of Australia where it was introduced as a garden plant. In Italy, P. myrtifolia is propagated by cuttings in commercial nurseries during the summer. In the winter of 2002, plants of P. myrtifolia growing in pots in an ornamental nursery in Sicily showed virus-like mosaic and malformation of leaves that appeared lanceolate with a lack of flowering. Leaf tissue was analyzed by double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) with polyclonal antisera to CMV, TSWV (Lettuce type), and INSV. Positive ELISA results were obtained only with the CMV polyclonal antisera. Complete remission of symptoms was observed on new flushes after pruning and incubation of infected plants at warm temperatures (30 and 20°C, day and night, respectively). This evidence led to the hypothesis that the disease or virus was disseminated by transportation and propagation of plants without visible symptoms during the hot season. A survey was also performed in two historical gardens of Catania (Sicily) where a group of apparently healthy P. myrtifolia plants, from the previously mentioned ornamental nursery in Sicily, were introduced as a single specimen or to form a hedge. These plants showed the same leaf malformations and mosaic symptoms observed in the nursery. DAS-ELISA confirmed the presence of CMV but not TSWV and INSV. To our knowledge, this is the first report of CMV on P. myrtifolia and it adds a new host to over 1,000 species (85 plant families) infected by this virus. Reference: (1) M. L. Daughtrey et al. Plant Dis. 81:1220, 1997.

scholarly journals First Report of Cucumber mosaic virus in Garlic Mustard in Ohio

Plant Disease ◽  
2000 ◽  
Vol 84 (9) ◽  
pp. 1047-1047 ◽  
Author(s):  
M. J. Boehm ◽  
S. T. Nameth
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Plant Species ◽  
Enzyme Linked Immunosorbent Assay ◽  
Garlic Mustard ◽  
Weed Species ◽  
Alternate Host ◽  
First Report ◽  
Molecular Weights ◽  
Symptomatic Tissue

Garlic mustard (Alliaria officinalis) is a common weed species associated with woodland borders, hedge rows, and suburban gardens. Garlic mustard plants expressing foliar symptoms of leaf mosaic and vein banding were collected from Franklin and Cuyahoga counties in Ohio. Analysis of symptomatic tissue using viral-associated double-stranded RNA (dsRNA) analysis on 5% polyacrylamide gels and stained with ethidium bromide resulted in the production of a banding profile (four dsRNA bands with molecular weights of 2.6, 2.0, 1.5, and 0.7 × 106 daltons) similar to that of Cucumber mosaic virus (CMV) (1). Symptomatic tissue suspected of being infected with CMV was analyzed with an indirect enzyme-linked immunosorbent assay (iELISA) employing commercially produced antiserum (Agdia Inc.) against the common strain of CMV antiserum confirmed the presence of CMV. Nonsymptomatic tissue reacted negatively to CMV. This is the first report of CMV in garlic mustard in Ohio. Due to the extensive range of this weed and the wide host range of CMV in ornamental and food-plant species, garlic mustard could serve as an alternate host for CMV in many commercially important plant species. Reference: (1) T. J. Morris et al. Plant Mol. Biol. Rep. 1:27–30, 1983.

scholarly journals First Report of Cucumber mosaic virus on Jatropha curcas in India

Plant Disease ◽  
2008 ◽  
Vol 92 (1) ◽  
pp. 171-171 ◽  
Author(s):  
S. K. Raj ◽  
S. Kumar ◽  
S. K. Snehi ◽  
U. Pathre
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Jatropha Curcas ◽  
Leaf Tissue ◽  
Mosaic Disease ◽  
Biodiesel Fuel ◽  
Rt Pcr ◽  
Specific Primers ◽  
First Report ◽  
Gel Diffusion

Jatropha curcas L. is a major commercial biodiesel fuel crop grown on 98 million acres (39.66 million ha) in India. Severe mosaic disease accompanied by yellow spots was noticed on 15% of J. curcas growing in the experimental plots of NBRI, Lucknow, India, during October of 2006. Inoculations with sap from symptomatic plants resulted in systemic mosaic on three of seven J. curcas seedlings. Gel diffusion tests were performed with antiserum to Cucumber mosaic virus (CMV), Tobacco ringspot virus, and Chrysanthemum virus B (PVAS-242a, PVAS-157, and PVAS-349, respectively; ATCC, Manassas, VA). Leaf sap of infected plants reacted only with PVAS-242a, indicating the presence of CMV. Reverse transcription (RT)-PCR assays with CMV coat protein gene specific primers (Genbank Accession Nos. AM180922 and AM180923) and total nucleic acid extracted from symptomatic J. curcas leaf tissue yielded the expected ~650-bp amplicon, which was cloned and sequenced (GenBank Accession No. EF153739). BLAST analysis indicated 98 to 99% nucleotide identity with CMV isolates (GenBank Accession Nos. DQ914877, DQ640743, AF350450, AF281864, X89652, AF198622, DQ152254, DQ141675, and DQ028777). Phylogenetic analysis showed that the J. curcas isolate was more closely related to Indian isolates of CMV belonging to subgroup Ib. Literature surveys revealed records of Jatropha mosaic virus on J. gossypiifolia in Puerto Rico (1) and on J. curcas in India (2). To our knowledge, this is the first report of CMV on J. curcas. References: (1) J. K. Brown et al. Arch. Virol. 146:1581, 2001. (2) D. S. A. Narayana et al. Curr. Sci. 91:584, 2006.

Identification of Three Distinct Classes of Satellite RNAs Associated With Two Cucumber mosaic virus Serotypes from the Ornamental Groundcover Vinca minor

2012 ◽  
Vol 13 (1) ◽  
pp. 18 ◽  
Author(s):  
John R. Fisher
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
The United States ◽  
Enzyme Linked Immunosorbent Assay ◽  
Rt Pcr ◽  
Pcr Cloning ◽  
Cloning And Sequencing ◽  
First Report ◽  
Vinca Minor ◽  
Satellite Rnas

Cucumber mosaic virus (CMV) is a cosmopolitan virus which may also have small satellite RNAs (satRNA) associated with it affecting symptom development. Vinca minor (periwinkle) plants exhibiting subtle mosaic symptoms tested positive for CMV by enzyme linked immunosorbent assay (ELISA). Double-stranded ribonucleic acid (dsRNA) analysis of CMV-Vinca field isolates in Nicotiana tabacum ‘Glurk’ suggested two sizes of putative satRNA associated with CMV. Immunocapture RT-PCR, cloning, and sequencing of the movement protein, coat protein, and satRNAs demonstrated serogroup 1A and serogroup 2 CMV helper strains and three distinct classes of satRNAs of four sizes. Further, two classes of satRNAs could be distinguished by their necrosis domains. Previously CMV was reported in V. minor in New Jersey. This is the first report of CMV in V. minor in Ohio and the first report of satRNA associated with CMV in V. minor in the United States. Accepted for publication 1 February 2012. Published 12 April 2012.

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