scholarly journals Polygala myrtifolia as a New Natural Host of Cucumber mosaic virus

Plant Disease ◽  
2002 ◽  
Vol 86 (12) ◽  
pp. 1403-1403 ◽  
Author(s):  
M. Tessitori ◽  
A. Reina ◽  
V. Catara ◽  
G. Polizzi
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Invasive Plant ◽  
Leaf Tissue ◽  
Impatiens Necrotic Spot Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
New Host ◽  
Polyclonal Antisera ◽  
Polygala Myrtifolia ◽  
Das Elisa

Cucumber mosaic virus (CMV), Tomato spotted wilt virus (TSWV), and Impatiens necrotic spot virus (INSV) are among the most important viral pathogens of ornamental plants (1). Polygala myrtifolia L. (myrtle-leaf milkwort), originating from South Africa, and a member of the Polygalaceae, was recently introduced in Italy as a cultivated ornamental shrub for its fast and attractive free-flowering growth and drought-resistant characteristics. It can become an invasive plant and is now considered a serious problem in coastal areas of Australia where it was introduced as a garden plant. In Italy, P. myrtifolia is propagated by cuttings in commercial nurseries during the summer. In the winter of 2002, plants of P. myrtifolia growing in pots in an ornamental nursery in Sicily showed virus-like mosaic and malformation of leaves that appeared lanceolate with a lack of flowering. Leaf tissue was analyzed by double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) with polyclonal antisera to CMV, TSWV (Lettuce type), and INSV. Positive ELISA results were obtained only with the CMV polyclonal antisera. Complete remission of symptoms was observed on new flushes after pruning and incubation of infected plants at warm temperatures (30 and 20°C, day and night, respectively). This evidence led to the hypothesis that the disease or virus was disseminated by transportation and propagation of plants without visible symptoms during the hot season. A survey was also performed in two historical gardens of Catania (Sicily) where a group of apparently healthy P. myrtifolia plants, from the previously mentioned ornamental nursery in Sicily, were introduced as a single specimen or to form a hedge. These plants showed the same leaf malformations and mosaic symptoms observed in the nursery. DAS-ELISA confirmed the presence of CMV but not TSWV and INSV. To our knowledge, this is the first report of CMV on P. myrtifolia and it adds a new host to over 1,000 species (85 plant families) infected by this virus. Reference: (1) M. L. Daughtrey et al. Plant Dis. 81:1220, 1997.

scholarly journals Lamium maculatum is a Natural Host for Cucumber mosaic virus

Plant Disease ◽  
2013 ◽  
Vol 97 (1) ◽  
pp. 150-150 ◽  
Author(s):  
R. Bešta-Gajević ◽  
A. Jerković-Mujkić ◽  
S. Pilić ◽  
I. Stanković ◽  
A. Vučurović ◽  
...  
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Impatiens Necrotic Spot Virus ◽  
Natural Occurrence ◽  
Rt Pcr ◽  
Natural Ecosystems ◽  
New Host ◽  
Total Rna ◽  
Negative Controls ◽  
Das Elisa

Lamium maculatum L. (spotted dead-nettle) is a flowering perennial ornamental that is commonly grown as a landscape plant for an effective ground cover. In June 2010, severe mosaic accompanied by reddish brown necrosis and leaf deformation was noticed on 80% of L. maculatum growing in shade under trees and shrubs in Sarajevo (Bosnia and Herzegovina). Leaves from 10 symptomatic L. maculatum plants were sampled and analyzed by double-antibody sandwich (DAS)-ELISA using commercial diagnostic kits (Bioreba AG, Reinach, Switzerland) against Cucumber mosaic virus (CMV), Tomato spotted wilt virus (TSWV), and Impatiens necrotic spot virus (INSV), the most important viral pathogens of ornamental plants (1,2). Commercial positive and negative controls and extracts from healthy L. maculatum leaves were included in each assay. All samples tested negative for TSWV and INSV and positive for CMV. The virus was mechanically transmitted to test plants and young virus-free plants of L. maculatum using 0.01 M phosphate buffer (pH 7). The virus caused chlorotic local lesions on Chenopodium quinoa, while systemic mosaic was observed on Capsicum annuum ‘Rotund,’ Nicotiana rustica, N. glutinosa, N. tabacum ‘White Burley,’ and Phaseolus vulgaris ‘Top Crop.’ The virus was transmitted mechanically to L. maculatum and induced symptoms resembling those observed on the source plants. Inoculated plants were assayed by DAS-ELISA and all five inoculated plants of each species tested positive for CMV. The presence of CMV in L. maculatum as well as mechanically infected N. glutinosa plants was further confirmed by RT-PCR. Total RNA from symptomatic leaves was isolated using RNeasy Plant Mini Kit (Qiagen, Hilden, Germany) and RT-PCR was performed with the One-Step RT-PCR Kit (Qiagen) following the manufacturer's instructions. The primer pair, CMVAu1u/CMVAu2d, that amplifies the entire coat protein (CP) gene and part of 3′- and 5′-UTRs was used for both amplification and sequencing (4). Total RNA obtained from the Serbian CMV isolate from pumpkin (GenBank Accession No. HM065510) and a healthy L. maculatum plant were used as positive and negative controls, respectively. All naturally and mechanically infected plants as well as the positive control yielded an amplicon of the expected size (850 bp). No amplicon was observed in the healthy control. The amplified product derived from isolate 3-Lam was purified (QIAquick PCR Purification Kit, Qiagen), directly sequenced in both directions and deposited in GenBank (JX436358). Sequence analysis of the CP open reading frame (657 nt), conducted with MEGA5 software, revealed that the isolate 3-Lam showed the highest nucleotide identity of 99.4% (99.1% amino acid identity) with CMV isolates from Serbia, Australia, and the USA (GQ340670, U22821, and U20668, respectively). To our knowledge, this is the first report of the natural occurrence of CMV on L. maculatum worldwide and it adds a new host to over 1,241 species (101 plant families) infected by this virus (3). This is also an important discovery for the ornamental industry since L. maculatum is commonly grown together with other ornamental hosts of CMV in nurseries and the urban environment as well as in natural ecosystems. References: (1) Y. K. Chen et al. Arch. Virol. 146:1631, 2001. (2) M. L. Daughtrey et al. Plant Dis. 81:1220, 1997. (3) M. Jacquemond. Adv. Virus Res. 84:439, 2012. (4) I. Stankovic et al. Acta Virol. 55:337, 2011.

scholarly journals First Report of Cucumber mosaic virus Infecting Solanum jasminoides in Italy

Plant Disease ◽  
2008 ◽  
Vol 92 (11) ◽  
pp. 1585-1585 ◽  
Author(s):  
S. Davino ◽  
F. Di Serio ◽  
G. Polizzi ◽  
M. Tessitori
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Sandwich Elisa ◽  
Leaf Tissue ◽  
Potato Spindle Tuber Viroid ◽  
Impatiens Necrotic Spot Virus ◽  
Rt Pcr ◽  
New Host ◽  
First Report ◽  
Natural Hosts

Solanum jasminoides Paxton (potato vine or jasmine nightshade) is a vegetatively propagated ornamental species within the Solanaceae family. Recently, symptomless plants of this species were reported as natural hosts of the quarantine pest, Potato spindle tuber viroid (PSTVd) in Italy (1). In January 2008, approximately 1,000 potted, 2-year-old plants of S. jasminoides growing in an ornamental nursery in Sicily showed virus-like mosaic and malformation of leaves. Symptoms were observed on approximately 60% of the plants. Leaf tissue, collected from 30 symptomatic and 10 symptomless plants, was analyzed by double-antibody sandwich-ELISA with polyclonal antisera specific to Cucumber mosaic virus (CMV), Tomato spotted wilt virus, and Impatiens necrotic spot virus (Loewe Biochemica, Sauerlach, Germany). The same samples were also analyzed by tissue-printing hybridization with a PSTVd-specific digoxigenin-labelled riboprobe. All the symptomatic samples tested positive only with antisera against CMV, but negative in all other tests. The symptomless samples were negative in all the performed tests. To confirm the association of CMV with the diseased plants, total RNA was extracted from the same samples (RNeasy Plant Mini Kit; Qiagen, Hilden, Germany) and analyzed by reverse transcription (RT)-PCR using CMV-specific primers MP+5′-CATGGCTTTCCAAGGTACCAG-3′ and MP-5′-CTAAAGACCGTTAACCACCTGC-3′ that amplify a 844-bp fragment from the MP gene (2). The expected fragment was amplified only from samples of symptomatic tissue. CMV was also detected in mother plants grown in the same nursery and showing same mosaic symptoms. Definitive identification of the pathogen was obtained by cloning and sequencing the RT-PCR product. The obtained sequence (GenBank Accession No. EU828783) had 99 and 98% similarity with the subgroup I-A isolates CMV-LUN (GenBank Accession No. EU432183) and CMV-Fny (GenBank Accession No. DI0538), respectively. To our knowledge, this is the first report of CMV infecting S. jasminoides and it adds a new host to the more than 1,000 species (85 plant families) infected by this virus. The high incidence of the disease in the nursery could be due to propagation of cuttings from an infected source. References: (1) F. Di Serio. J. Plant Pathol. 89:297, 2007. (2) H. X. Lin et al. J. Virol. 78:6666, 2004.

Dalmatian Toadflax (Linaria dalmatica): New Host for Cucumber Mosaic Virus

2007 ◽  
Vol 21 (1) ◽  
pp. 41-44 ◽  
Author(s):  
Courtney L. Pariera Dinkins ◽  
Sue K. Brumfield ◽  
Robert K. D. Peterson ◽  
William E. Grey ◽  
Sharlene E. Sing
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Aphid Transmission ◽  
Negative Control ◽  
Enzyme Linked Immunosorbent Assay ◽  
New Host ◽  
Tobacco Plants ◽  
Linaria Dalmatica ◽  
Mechanical Methods ◽  
Dalmatian Toadflax

To date, there have been no reports of Dalmatian toadflax serving as a host for cucumber mosaic virus (CMV). Infestations of Dalmatian toadflax may serve as a reservoir of CMV, thereby facilitating aphid transmission of CMV to both agricultural crops and native plants. The goal of this study was to determine whether Dalmatian toadflax is a host for CMV. Dalmatian toadflax seedlings were randomly assigned to two treatments (18 replicates/treatment): no inoculation (control) and inoculation with CMV (Fast New York strain). The Dalmatian toadflax seedlings were inoculated by standard mechanical methods and tested for the presence of CMV using enzyme-linked immunosorbent assay (ELISA). Ten of the 18 CMV-inoculated toadflax plants tested positive for the virus; 6 of the 18 displayed systemic mosaic chlorosis and leaf curling. All control plants tested negative. Transmission electron microscopy obtained from CMV-positive plants confirmed the presence of CMV based on physical properties. To verify CMV infestation, tobacco plants were assigned to the following treatments (six replicates/treatment): no inoculation (control), CMV-negative (control) inoculation, and a CMV-positive inoculation. Plants were inoculated by standard methods. Five of the 6 tobacco plants treated with the CMV-positive inoculum tested positive for CMV using ELISA. All control plants tested negative for the virus.

scholarly journals First Report of Cucumber mosaic virus Infecting Peperomia tuisana in Serbia

Plant Disease ◽  
2013 ◽  
Vol 97 (7) ◽  
pp. 1004-1004 ◽  
Author(s):  
K. Milojević ◽  
I. Stanković ◽  
A. Vučurović ◽  
D. Ristić ◽  
D. Milošević ◽  
...  
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Disease Incidence ◽  
Impatiens Necrotic Spot Virus ◽  
Rt Pcr ◽  
Test Species ◽  
First Report ◽  
Cp Gene ◽  
Negative Controls ◽  
Das Elisa

Peperomia tuisana C.DC. ex Pittier (family Piperaceae) is an attractive succulent grown as an ornamental. Despite its tropical origins, it can be successfully grown indoors in any climate. In March 2012, three samples of P. tuisana showing virus-like symptoms were collected from a commercial greenhouse in Zemun (District of Belgrade, Serbia) in which estimated disease incidence was 80%. Infected plants showed symptoms including necrotic ringspots and line patterns that enlarged and caused necrosis of leaves. A serious leaf drop led to growth reduction and even death of the plant. Leaves from three symptomatic P. tuisana plants were sampled and analyzed by double-antibody sandwich (DAS)-ELISA using commercial diagnostic kits (Bioreba AG, Reinach, Switzerland) against the most common viral pathogens of ornamentals: Cucumber mosaic virus (CMV), Tomato spotted wilt virus (TSWV), and Impatiens necrotic spot virus (INSV) (1,2). Commercial positive and negative controls were included in each ELISA. Serological analyses showed that all plants were positive for CMV and negative for TSWV and INSV. The ELISA-positive sample (isolate 1-12) was mechanically inoculated onto five plants each of three test species as well as of healthy young P. tuisana using 0.01 M phosphate buffer (pH 7). Chlorotic local lesions on Chenopodium quinoa and severe mosaic and leaf malformations were observed on all inoculated Nicotiana tabacum ‘Samsun’ and N. glutinosa. Also, the virus was successfully mechanically transmitted to P. tuisana that reacted with symptoms identical to those observed on the original host plants. All mechanically inoculated plants were positive for CMV in DAS-ELISA. For further confirmation of CMV infection, reverse transcription (RT)-PCR was performed on extracts made from symptomatic P. tuisana, N. tabacum ‘Samsun,’ and N. glutinosa leaf materials. Total RNAs were extracted with the RNeasy Plant Mini Kit (Qiagen, Hilden, Germany) and RT-PCR was carried out using One-Step RT-PCR Kit (Qiagen). A CMV-specific primer pair, CMVCPfwd and CMVCPrev (3), which amplifies an 871-bp fragment of the entire coat protein (CP) gene and part of 3′- and 5′-UTRs, were used for both amplification and sequencing. Total RNAs obtained from the Serbian CMV isolate (HM065510) and healthy P. tuisana were used as positive and negative controls, respectively. A product of the correct predicted size was obtained in all naturally and mechanically infected plants, as well as positive control. No amplicon was recorded in the healthy control. The amplified product derived from isolate 1-12 was purified (QIAquick PCR Purification Kit, Qiagen), directly sequenced in both directions, deposited in GenBank (KC505441), and analyzed by MEGA5 software (4). Sequence comparison of the complete CP gene (657 nt) revealed that the Serbian isolate 1-12 shared the highest nucleotide identity of 99.1% (99.5% amino acid identity) with the Japanese isolate (AB006813). To our knowledge, this is the first report on the occurrence of CMV in P. tuisana in Serbia. This is also an important discovery since P. tuisana is commonly grown together with other ornamental hosts of CMV, and thus could represent a serious threat for future expansion of CMV in the greenhouse floriculture industry in Serbia. References: (1) M. L. Daughtrey et al. Plant Dis. 81:1220, 1997. (2) S. Flasinski et al. Plant Dis. 79:843, 1995. (3) K. Milojevic et al. Plant Dis. 96:1706, 2012. (4) K. Tamura et al. Mol. Biol. Evol. 28:2731, 2011.

scholarly journals First Report of a Cucumber mosaic virus-Associated Satellite RNA in Lobelia erinus

Plant Disease ◽  
2001 ◽  
Vol 85 (7) ◽  
pp. 802-802 ◽  
Author(s):  
S. G. P. Nameth ◽  
J. R. Fisher
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Leaf Tissue ◽  
Late Summer ◽  
Culture Collection ◽  
Northern Hybridization ◽  
Enzyme Linked Immunosorbent Assay ◽  
Satellite Rna ◽  
First Report ◽  
Dna Labeling

Lobelia (Lobelia erinus L.) is a common herbaceous annual used in flower beds and hanging baskets. The plant blooms from early to late summer. In the summer of 2000, Lobelia plants expressing virus-like symptoms were collected from a greenhouse-based production site in Ohio. Affected plants expressed a mild leaf mosaic and stunting. Viral-associated dsRNA was isolated from 7 g of symptomatic leaf tissue (1). Four dsRNAs were observed at 3.9, 3.0, 2.25, and 1.05 kb indicating the presence of a Cucumovirus. A fifth dsRNA at 0.75 kb also was observed, consistent with the presence of a satellite RNA. Enzyme-linked immunosorbent assay (ELISA) analysis (Agdia, Inc., Elkhart, IN) of symptomatic Lobelia tissue confirmed the presence of Cucumber mosaic virus (CMV). A (S)CARNA-5 (-) cDNA clone (American Type Culture Collection #45124) was labeled with digoxygenin (DIG) as per the manufacturer's instructions (Genius II DIG-DNA Labeling Kit, Boehringer Mannheim) and used as a diagnostic probe to detect this satellite RNA. Northern hybridization confirmed the identity of the satellite RNA (2). This is the first report of any satellite RNA associated with a virus infection in Lobelia and the first report of CMV in this host in Ohio. References: (1) J. R. Fisher and S. G. P. Nameth. HortScience 35:230–234, 2000. (2) R. A. Valverde et.al. Plant Dis. 74:255–258, 1990.

scholarly journals Spathiphyllum sp.: A New Natural Host of Impatiens necrotic spot virus

Plant Disease ◽  
2001 ◽  
Vol 85 (4) ◽  
pp. 448-448 ◽  
Author(s):  
A. Materazzi ◽  
E. Triolo
Keyword(s):  
Mosaic Virus ◽  
Alfalfa Mosaic Virus ◽  
Systemic Infection ◽  
Impatiens Necrotic Spot Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Necrotic Spot ◽  
Spot Virus ◽  
New Host ◽  
Arabis Mosaic Virus ◽  
Wilt Virus

In September 1999, several Spathiphyllum plants grown in a greenhouse in Tuscany (Italy) showed leaf symptoms in the form of concentric chlorotic ringspots, line patterns, and irregular chlorotic blotches. These symptoms developed into localized necrosis. Crude sap of tissues showing symptoms was mechanically inoculated to young symptomless Spathiphyllum plants and to Nicotiana benthamiana and N. clevelandii. Samples drawn from symptomatic and symptomless tissues of naturally or artificially infected Spathiphyllum and Nicotiana plants were tested for the presence of Alfalfa mosaic virus (AMV), Arabis mosaic virus (ArMV), Cucumber mosaic virus (CMV), Dasheen mosaic virus (DsMV), Impatiens necrotic spot virus (INSV), Potato X virus (PVX), Potato Y virus (PVY), Tobacco mosaic virus (TMV), and Tomato spotted wilt virus (TSWV) by double-antibody sandwich enzyme-linked immunosorbent assay carried out with commercial antisera. The symptomatic tissues obtained from Spathiphyllum and Nicotiana plants gave a positive reaction only for INSV. The symptomless samples obtained from various parts of the infected Spathiphyllum plants gave a negative reaction, even after 1 year from the appearance of localized necrosis, suggesting a non-systemic infection in this new host. This is the first report of infection of Spathiphyllum sp. by INSV.

scholarly journals Two New Hosts for Poinsettia Mosaic Virus

Plant Disease ◽  
1999 ◽  
Vol 83 (4) ◽  
pp. 399-399 ◽  
Author(s):  
E. Floeistad ◽  
D. R. Blystad
Keyword(s):  
Mosaic Virus ◽  
Ricinus Communis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Euphorbia Pulcherrima ◽  
Graft Union ◽  
New Host ◽  
Ricinus Communis L ◽  
Symptomless Infection ◽  
New Hosts ◽  
Das Elisa

Poinsettia mosaic virus (PnMV), a possible member of the genus Tymovirus, commonly infects the potted flower crop Euphorbia pulcherrima Willd. ex Klotzsch (1). Two new host species for this virus were identified during grafting experiments with E. pulcherrima and other Euphorbia spp. E. cornastra (Dressler) A. Radcliffe-Smith was reciprocally grafted with PnMV-positive E. pulcherrima cv. Eckespoint Lilo. PnMV was detected by double antibody sandwich-enzyme-linked immunosorbent assay (DAS-ELISA) in E. cornastra leaves directly below the graft union 4 weeks after grafting. Infection was not fully systemic 6 weeks after grafting when screened by DAS-ELISA with antibodies specific to PnMV (DSMZ, Braunschweig, Germany). The symptomless infection in E. cornastra persisted in cuttings from grafted plants after a 1-year observation period. E. bubalina Boiss. anatomy differs from that of E. pulcherrima. The two species did not produce a viable graft union. However, in an experiment with two attempted graftings, the E. pulcherrima scions remained turgid for 14 to 18 days. As a result of one grafting, the E. bubalina rootstock tested positive for PnMV. The virus induced a mild mosaic in E. bubalina, but no reduction in growth. To confirm virus presence in E. cornastra and E. bubalina, both DAS-ELISA and immunosorbent electron microscopy were used. Non-grafted controls remained PnMV negative. PnMV was re-isolated from both species by sap inoculation to Nicotiana benthamiana. E. coulescens Haw., E. xylophyllides Brogn. ex Lem., E. marlothiana N. E. Br., and Ricinus communis L. were not infected by PnMV after similar grafting attempts. Reference: (1) A. A. Brunt et al., eds. 1996. Viruses of Plants. CAB Int., Wallingford, UK.

scholarly journals Mosaic Symptoms Induced by Cucumber mosaic virus in Polygala myrtifolia in France and New Zealand

Plant Disease ◽  
2005 ◽  
Vol 89 (5) ◽  
pp. 527-527 ◽  
Author(s):  
L. Cardin ◽  
J.-P. Onesto ◽  
B. Delecolle ◽  
B. Moury
Keyword(s):  
New Zealand ◽  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Polyclonal Antibodies ◽  
Virus Titer ◽  
Cut Flowers ◽  
Total Recovery ◽  
Voucher Specimen ◽  
Polygala Myrtifolia ◽  
Das Elisa

Myrtle-leaf milkwort or sweet pea shrub (Polygala myrtifolia L.), family Polygalaceae, is a shrub from South Africa and is well adapted to Mediterranean-type conditions and used as an ornamental plant in gardens and pots or as cut flowers. During 2002 and 2003, mosaic symptoms and leaf distortion were observed in P. myrtifolia in Menton, Roquebrune-Cap Martin, Golfe Juan, and Antibes (Alpes Maritimes Department, France) in public gardens and potted plants. Occasionally, white streaks were observed in flowers. Cucumber mosaic virus (CMV) was identified in samples collected from the four locations on the basis of transmission to and symptoms exhibited by a range of diagnostic host plants (1), observation of isometric particles (≅30 nm) in crude sap preparations from the infected plants by electron microscopy, and positive reaction using double-antibody sandwich enzyme-linked immunosorbent assays (DAS-ELISA) with polyclonal antibodies raised against CMV (2). Each isolate was shown to be a group II CMV strain (3) using double-immunodiffusion analysis. During 2004, CMV was also detected using DAS-ELISA in P. myrtifolia samples collected in New Zealand (Christchurch, Akaroa, and Roturoa). To confirm that CMV was responsible for pathogenicity, the Menton isolate was isolated from local lesions on Vigna unguiculata, amplified in Nicotiana tabacum cv. Xanthi-nc, and then mechanically inoculated into 1-year-old P. myrtifolia, P. myrtifolia cv. Grandiflora, and P. myrtifolia cv. Compacta (synonymous to cv. Nana) plants. The D strain of CMV, a reference tomato strain from subgroup I (2), was used for comparison. All experimental plants were propagated from cuttings, grown hydroponically and all tested negative for CMV using DAS-ELISA prior to inoculation. At 12 weeks postinoculation, systemic symptoms were observed on leaves from all inoculated plants (10 plants per genotype for the Menton isolate and 5 plants per genotype for the D strain), except for two P. myrtifolia plants inoculated with the Menton isolate. CMV was detected in apical, noninoculated leaves using DAS-ELISA in all symptomatic plants. A total recovery from symptoms was observed in P. myrtifolia and P. myrtifolia cv. Grandiflora but not in P. myrtifolia cv. Compacta at 6 months postinoculation (mpi) in 7 of 15, 10 of 15, and 15 of 15 DAS-ELISA positive plants, respectively. At 7 mpi, the plants were pruned and planted in soil and at 8 mpi, CMV was detected using DAS-ELISA in most of the plants, and symptoms developed in a few stems of some of the plants. Tessitori et al. (4) described similar symptoms and have detected CMV in P. myrtifolia from Italy, but they did not reproduce the disease in healthy plants. Our results show that CMV is responsible for the symptoms observed and that both CMV subgroups are infectious in P. myrtifolia. Since P. myrtifolia is generally vegetatively propagated by cuttings, frequent CMV tests on the mother stock plants are recommended because of fluctuations in virus titer and symptom expression in some genotypes. To our knowledge, this is the first report of this CMV host in France and New Zealand. A voucher specimen will be deposited at the Station de Pathologie Végétale at INRA, Montfavet. References: (1) L. Cardin et al. Plant Dis. 87:1263, 2003. (2) J. C. Devergne and L. Cardin. Ann. Phytopathol. 7:225, 1975. (3) M. J. Roossinck. J. Virol. 76:3382, 2002. (4) M. Tessitori et al. Plant Dis. 86:1403, 2002.

scholarly journals Occurrence of Cucumber mosaic virus Infecting Parsley (Petroselinum crispum) in Turkey

2011 ◽  
Vol 39 (1) ◽  
pp. 30 ◽  
Author(s):  
Mehmet Ali SEVIK ◽  
Cemile AKCUCURA
Keyword(s):  
Virus Infection ◽  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Petroselinum Crispum ◽  
Black Sea Region ◽  
Public Markets ◽  
Winter Crops ◽  
Leaf Distortion ◽  
Das Elisa

Parsley plants are grown throughout Turkey as summer and winter crops. Diseased plants having typical of a virus infection such as mosaic, mottling, and leaf distortion symptoms were frequently observed in most of the parsley fields and vegetable public markets in the Middle Black Sea Region of Turkey in 2010. Using double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA), Cucumber mosaic virus (CMV) was detected on the diseased parsley plants. However, using farmers and commercial seed lots, CMV was not detected in seeds or germinating seedlings.

scholarly journals UJI KETAHANAN TIGA VARIETAS TOMAT (SOLANUM LYCOPERSICUM L.) TERHADAP SERANGAN CMV (CUCUMBER MOSAIC VIRUS) DENGAN METODE DAS-ELISA

2017 ◽  
Vol 1 (2) ◽  
pp. 101
Author(s):  
Lia Agturani Tudaryati ◽  
Febi Nurilmala ◽  
Krisna Dwiharniati
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Solanum Lycopersicum ◽  
Incubation Period ◽  
Positive Control ◽  
Elisa Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Tomato Plants ◽  
Solanum Lycopersicum L ◽  
Das Elisa

Endurance Test Three Tomato Variety (Solanum lycopersicum L.) Against Attacks CMV (Cucumber Mosaic Virus) DAS-ELISA Method          Tomato plantation intensification can be done by controlling nuisance organism plant causes disease in tomato, such as CMV. CMV can be transmitted mechanically, and therefore testing of tomato varieties resistance to CMV can be performed with CMV isolates infect mechanically to healthy tomato plants (variety Marta F1, San Marino, and Viccario). CMV isolates derived from two sources, namely a positive tomato plants attacked by CMV (plant sap) and the positive control CMV AGDIA producers commonly used as a positive control test DAS-ELISA (Double Antibody Sandwich-Enzyme Linked immunosorbent assay). This study aims to test three varieties of tomato resistance against CMV attacks. Research conducted in the screen house and laboratory virology Central Agricultural Quarantine Standards Test. Identification of CMV infection was done by observing the incubation period, symptoms appeared, and continued with DAS-ELISA test. Structured treatment completely randomized design (CRD) with 6 replications. Data processed with fingerprint analysis and continued with various multiple Duncan test at 5% level test. The results showed the disease symptoms appeared only on the varieties of San Marino from CMV infected plant sap. Symptoms caused a reduction of leaf rolling and leaf lamina 8-10 day incubation period and symptoms of stunted stems with 14-28 day incubation period, the San Marino CMV causes the reduction of plant height by 33%. Sensitivity of tomato varieties against CMV San Marino sap from plants was quantitatively evidenced by positive results on the DAS-ELISA test. Meanwhile, F1 and Marta varieties resistant to Viccario CMV infection sap from plants and the positive control CMV AGDIA producers. Keywords : Tomato (Solanum Lycopersicum l.), Cucumber Mosaic Virus, DAS-ELISA method ABSTRAK                 Intensifikasi perkebunan tomat dapat dilakukan dengan mengendalikan organisme pengganggu tanaman (OPT) penyebab penyakit pada tomat, seperti CMV. CMV dapat ditularkan secara mekanis, oleh karena itu pengujian ketahanan varietas tomat terhadap CMV dapat dilakukan dengan menularkan isolat CMV secara mekanik kepada tanaman tomat sehat (varietas Marta F1, San Marino, dan Viccario). Isolat CMV berasal dari dua sumber, yaitu tanaman tomat yang positif terserang CMV (sap tanaman) dan kontrol positif CMV produsen AGDIA yang biasa digunakan sebagai kontrol positif pengujian DAS-ELISA (Double Antibody Sandwich-Enzyme Linked Immunosorbent assay). Penelitian ini bertujuan untuk menguji ketahanan tiga varietas tomat terhadap serangan CMV. Penelitian dilaksanakan di screen house dan laboratorium virologi Balai Besar Uji Standar Karantina Pertanian pada bulan Januari sampai April 2008. Identifikasi hasil penularan CMV dilakukan dengan mengamati periode inkubasi, gejala yang muncul, dan dilanjutkan dengan pengujian DAS-ELISA. Perlakuan disusun dengan rancangan acak lengkap (RAL) dengan 6 ulangan. Data diolah dengan analisis sidik ragam dan dilanjutkan dengan uji berganda Duncan pada taraf uji 5%. Hasil penelitian menunjukkan gejala penyakit hanya muncul pada varietas San Marino yang ditulari CMV asal sap tanaman. Gejala yang ditimbulkan berupa daun menggulung dan reduksi lamina daun dengan masa inkubasi 8-10 hari, serta gejala batang kerdil dengan masa inkubasi 14-28 hari, CMV pada San Marino menyebabkan reduksi tinggi tanaman sebesar 33%. Kesensitifan tomat varietas San Marino terhadap CMV asal sap tanaman secara kuantitatif dibuktikan dengan hasil yang positif pada pengujian DAS-ELISA. Sedangkan, varietas Marta F1 dan Viccario tahan terhadap penularan CMV asal sap tanaman maupun kontrol positif CMV produsen AGDIA.Kata kunci : Tomat (Solanum lycopersicum  L.), Cucumber Mosaic Virus, metode DAS-ELISA

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