scholarly journals Screening and Field Trials of Virus Resistant Sources in Capsicum spp.

Plant Disease ◽  
2003 ◽  
Vol 87 (7) ◽  
pp. 779-783 ◽  
Author(s):  
Kazumi Suzuki ◽  
Tomohisa Kuroda ◽  
Yoshio Miura ◽  
Jun Murai
Keyword(s):  
Mosaic Virus ◽  
Field Tests ◽  
Field Trials ◽  
Hypersensitive Reaction ◽  
Tomato Mosaic Virus ◽  
Systemic Necrosis ◽  
Tomato Aspermy Virus ◽  
Tomato Spotted Wilt ◽  
Capsicum Spp ◽  
Wilt Virus

Thirty-seven Capsicum accessions containing cultivated and wild species were screened for resistance to Cucumber mosaic virus (CMV), and were also investigated for their response to Tomato aspermy virus (TAV), Tomato mosaic virus (ToMV), Pepper mild mottle virus (PMMoV), and Tomato spotted wilt virus (TSWV). C. baccatum PI 439381-1-3 (PI 439381-1-3), C. frutescens LS 1839-2-4 (LS 1839-2-4), and C. frutescens cv. Tabasco (cv. Tabasco) showed a hypersensitive reaction against CMV-Y, and thus were not systemically infected. Only inoculated leaves of C. annuum cv. Sapporo-oonaga and cv. Nanbu-oonaga were infected with CMV-Y, and viral infection did not spread systemically. These five accessions (PI 439381-1-3, LS 1839-2-4, cv. Tabasco, cv. Sapporo-oonaga, and cv. Nanbu-oonaga) were considered resistant to CMV-Y. These accessions were also resistant to other CMV isolates, but not to the TAV isolate. PI 439381-1-3, LS1839-2-4, cv. Sapporo-oonaga, and cv. Nanbu-oonaga were susceptible to PMMoV, while PI 439381-1-3 and LS1839-2-4 showed systemic necrosis. All CMV-resistant accessions were susceptible to TSWV. Field tests of eight Capsicum accessions, including CMV, PMMoV, and/or TSWV-resistant accessions, demonstrated that most of the PI 439381-1-3 plants were not infected with CMV and PMMoV among the virus-infested fields. As occurred with mechanical inoculation, LS 1839-2-4, cv. Tabasco, cv. Sapporo-oonaga, and cv. Nanbu-oonaga were hard to infect with CMV in the field.

scholarly journals New Breeding Lines Resistant to Tomato Mosaic Virus and Tomato Spotted Wilt Virus within the ‘De la Pera’ Tomato Type: UMH 1353 and UMH 1354

HortScience ◽  
2016 ◽  
Vol 51 (4) ◽  
pp. 456-458 ◽  
Author(s):  
Santiago García-Martínez ◽  
Adrián Grau ◽  
Aranzazu Alonso ◽  
Fernando Rubio ◽  
Pedro Carbonell ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Tomato Spotted Wilt Virus ◽  
Tomato Mosaic Virus ◽  
Breeding Lines ◽  
Tomato Spotted Wilt ◽  
Wilt Virus

Development of Reverse Transcription Thermostable Helicase-Dependent DNA Amplification for the Detection of Tomato Spotted Wilt Virus

2016 ◽  
Vol 99 (6) ◽  
pp. 1596-1599 ◽  
Author(s):  
Xinghai Wu ◽  
Chanfa Chen ◽  
Xizhi Xiao ◽  
Ming Jun Deng
Keyword(s):  
Reverse Transcriptase ◽  
Mosaic Virus ◽  
Reverse Transcription ◽  
Tomato Spotted Wilt Virus ◽  
Tomato Mosaic Virus ◽  
N Gene ◽  
Impatiens Necrotic Spot Virus ◽  
Rt Pcr ◽  
Tomato Spotted Wilt ◽  
Wilt Virus

Abstract A protocol for the reverse transcription-helicase-dependent amplification (RT–HDA) of isothermal DNA was developed for the detection of tomato spotted wilt virus (TSWV). Specific primers, which were based on the highly conserved region of the N gene sequence in TSWV, were used for the amplification of virus's RNA. The LOD of RT–HDA, reverse transcriptase-loop-mediated isothermal amplification (RT-LAMP), and reverse transcriptase-polymerase chain reaction (RT-PCR) assays were conducted using 10-fold serial dilution of RNA eluates. TSWV sensitivity in RT–HDA and RT-LAMP was 4 pg RNA compared with 40 pg RNA in RT-PCR. The specificity of RT–HDA for TSWV was high, showing no cross-reactivity with other tomato and Tospovirus viruses including cucumber mosaic virus (CMV), tomato black ring virus (TBRV), tomato mosaic virus (ToMV), or impatiens necrotic spot virus (INSV). The RT–HDA method is effective for the detection of TSWV in plant samples and is a potential tool for early and rapid detection of TSWV.

scholarly journals First Report of Tomato spotted wilt virus on Tobacco in Campania, Italy

Plant Disease ◽  
2011 ◽  
Vol 95 (5) ◽  
pp. 611-611 ◽  
Author(s):  
R. Carrieri ◽  
R. Sorrentino ◽  
E. Lahoz ◽  
D. Alioto
Keyword(s):  
Mosaic Virus ◽  
Tomato Spotted Wilt Virus ◽  
Agricultural Research ◽  
Sandwich Elisa ◽  
The United States ◽  
Systemic Necrosis ◽  
Nucleocapsid Gene ◽  
Datura Metel ◽  
Tomato Spotted Wilt ◽  
Wilt Virus

During the spring and summer of 2010, a survey for viruses was conducted in two tobacco field trials at the Agricultural Research and Experimentation Council in Scafati, Campania, Italy. A total of 1,392 symptomatic and asymptomatic tobacco plants (cv. Burley) were sampled, and leaves were analyzed by double-antibody sandwich-ELISA using polyclonal antisera against five tobacco-infecting viruses: Tobacco mosaic virus, Potato virus Y, Cucumber mosaic virus, Alfalfa mosaic virus, and Tomato spotted wilt virus (TSWV) (Loewe, Munich, Germany). Only one plant was positive to TSWV. Symptoms on this plant were severe necroses on the stem with a few chlorotic/necrotic leaflets on the top of the plant. This result was subsequently confirmed by reverse transcription (RT)-PCR. The primers (5′-ATGTCTAAGGTTAAGCTC-3′ forward and 5′-TTAAGCAAGTTCTGTGAG-3′ reverse) targeted the nucleocapsid gene of TSWV and amplified the expected product of approximately 800 bp (2). The resultant sequence (GenBank Accession No. JF290419) was aligned and edited using BlastN, displaying 99.9% identity with deposited TSWV nucleocapsid gene sequences in GenBank, with no similarity to any other targets, which confirmed the presence of TSWV in tobacco. Leaf homogenate from the tobacco symptomatic plants was inoculated onto three plants of Nicotiana benthamiana, N. glutinosa, and Datura metel. All plants developed a systemic necrosis after 7 days. Inoculation from N. glutinosa back to cv. White Burley tobacco produced symptoms similar to those observed in the field. Two plants from each species were used as noninoculated controls, which remained asymptomatic. TSWV infection has been responsible for severe epidemics on tobacco throughout the United States and Greece, with losses estimated as much as 85% (1,3). The presence of TSWV in Italy could therefore represent a serious threat for tobacco in the region, especially considering that it is prevalent in other crops in the area and vectors are widespread. References: (1) E. K. Chatzivassiliou. Plant Dis. 92:1012, 2008. (2) R. K. Jain et al. Plant Dis. 82:900, 1998. (3) B. Mandal et al. Ann. Appl. Biol. 151:67, 2007.

scholarly journals UMH 1422 and UMH 1415: Two Fresh-market Tomato Breeding Lines Resistant to Tomato Mosaic Virus and Tomato Spotted Wilt Virus

HortScience ◽  
2014 ◽  
Vol 49 (11) ◽  
pp. 1465-1466 ◽  
Author(s):  
Santiago García-Martínez ◽  
Adrián Grau ◽  
Aranzazu Alonso ◽  
Fernando Rubio ◽  
Manuel Valero ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Tomato Spotted Wilt Virus ◽  
Tomato Mosaic Virus ◽  
Fresh Market ◽  
Breeding Lines ◽  
Tomato Spotted Wilt ◽  
Tomato Breeding ◽  
Wilt Virus

scholarly journals Natural incidence of bean viruses in the northwest of Iran

2017 ◽  
Vol 109 (2) ◽  
pp. 331
Author(s):  
Mina Rastgou ◽  
Masoumeh Jalali
Keyword(s):  
Mosaic Virus ◽  
Bean Common Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Tomato Mosaic Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Growing Seasons ◽  
Tomato Spotted Wilt ◽  
Northwest Of Iran ◽  
Wilt Virus ◽  
Leaf Curl Virus

<p><span style="font-family: Times New Roman;">Bean is considered as one of the most important legumes around the world. Viral diseases are a major yield reducing factor in bean production. Bean samples with virus-like symptoms like severe or mild mosaic, vein banding, leaf curling, blistering and necrosis were collected from different bean fields in Urmia (Northwest of Iran) during the growing seasons of 2013 and 2014. <em>Bean common mosaic virus</em> (BCMV), <em>Bean common mosaic necrosis virus</em> (BCMNV), <em>Bean yellow mosaic virus</em> (BYMV), <em>Cucumber mosaic virus</em> (CMV), <em>Tomato spotted wilt virus</em> (TSWV), <em>Tomato mosaic virus</em> (ToMV) and <em>Tomato yellow leaf curl virus</em> (TYLCV) were detected by double antibody sandwich enzyme-linked-immunosorbent assay. Mixed infection of BCMV and BCMNV were found. BCMNV was the most frequent virus in this region whereas BYMV and TYLCV were each detected just in one sample. This is the first report of BCMNV, BCMV, BYMV, TSWV, TMV and TYLCV incidence on bean in Urmia, Iran.</span></p>

Effect of Imazapic and 2,4-DB on Peanut Yield, Grade, and Seed Germination

2010 ◽  
Vol 37 (1) ◽  
pp. 78-82 ◽  
Author(s):  
W. H. Faircloth ◽  
E. P. Prostko
Keyword(s):  
Seed Germination ◽  
Tomato Spotted Wilt Virus ◽  
Seed Quality ◽  
Field Trials ◽  
The Other ◽  
Peanut Seed ◽  
Stand Establishment ◽  
South Georgia ◽  
Tomato Spotted Wilt ◽  
Wilt Virus

Abstract Recurrent problems with peanut seed germination and stand establishment have raised concerns about the potential effects of herbicides on peanut seed quality. Field trials were conducted in south Georgia in 2005 and 2006 to evaluate the effects of imazapic and 2,4-DB on peanut yield, grade, incidence of tomato spotted wilt virus (TSWV), and seed germination. Imazapic at 71 g ai ha−1, applied at 30 and 40 days after planting (DAP), and 2,4-DB at 270 g ai ha−1, applied at 75 or 90 DAP, had no effect on peanut yield, grade, incidence of TSWV, and seed germination when averaged over two locations (Dawson and Tifton) and three cultivars (Georgia Green, Georgia-01R, and C-99R). Normal cultivar yield variations were observed; however, Georgia-01R had reduced standard (25 C) and cold (15 C) germination when compared to the other cultivars.

scholarly journals Incidence of viruses infecting pepper in Thailand

2019 ◽  
Vol 10 (1) ◽  
pp. 184-193
Author(s):  
Amisa Laprom ◽  
Somrudee Nilthong ◽  
Ekachai Chukeatirote
Keyword(s):  
Mosaic Virus ◽  
Mixed Infection ◽  
Tomato Spotted Wilt Virus ◽  
Potato Virus Y ◽  
Enzyme Linked Immunosorbent Assay ◽  
Mixed Infections ◽  
The North ◽  
Tomato Spotted Wilt ◽  
Wilt Virus ◽  
Chilli Veinal Mottle Virus

AbstractThis study was conducted to determine the incidence, diversity and distribution of viruses infecting pepper (Capsicum spp.) in the central, northern and northeastern parts of Thailand. During a survey in 2016 - 2019, a total of 2,149 leaf samples from symptomatic and asymptomatic peppers were collected randomly from farmer’s fields, and preliminary tested by an enzyme-linked immunosorbent assay (ELISA) using 7 antibodies specific for cucumber mosaic virus (CMV), chilli veinal mottle virus (ChiVMV), tomato necrotic ringspot virus (TNRV), tobacco mosaic virus (TMV), potato virus Y (PVY), tomato spotted wilt virus (TSWV), and begomoviruses. Our data revealed that the incidence of the viruses infecting pepper in Thailand was high, accounting for nearly 70% (1,482 infected samples). The highest viral incidence was found in the central part (96%), followed by the north (74.4%) and the northeastern (52.8%), respectively. Begomoviruses, CMV, ChiVMV, and TNRV were detected in the samples at varying rates, whereas PVY, TMV, and TSWV were not detected. Of these, the most frequently found virus was Begomoviruses accounting for nearly 33%, with the highest rate (ca. 82%) in the central Provinces of Thailand. In addition, of the 1,482 infected samples, mixed infections among the four viruses were also found in 616 samples (ca. 42%), and CMV + ChiVMV (approximately 11%) was the most common mixed infection. This is the first report describing an occurrence of viruses in pepper of Thailand, and the results obtained have revealed that viruses infecting pepper are widespread, which may pose a threat to pepper production in Thailand.

scholarly journals Bakterijske i virusne bolesti rajčice i paprike u Hrvatskoj

2019 ◽  
Vol 42 (3) ◽  
pp. 36-42
Author(s):  
Dario Ivić
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Pseudomonas Syringae ◽  
Tomato Spotted Wilt Virus ◽  
Clavibacter Michiganensis ◽  
Tomato Spotted Wilt ◽  
Wilt Virus

U proteklih nekoliko godina u Hrvatskoj su zabilježene relativno visoke štete od pojedinih bakterijskih i virusnih bolesti u proizvodnji rajčice i paprike. Uzročnici tih bolesti bile su bakterije Pseudomonas syringae pv. tomato i Clavibacter michiganensis ssp. michiganensis te virusi Tomato spotted wilt virus (TSWV) i Cucumber mosaic virus (CMV). U članku su ukratko opisani spomenuti patogeni, njihovo širenje te mjere zaštite.

scholarly journals Occurrence of viruses on pepper plantations in the Czech Republic – Short communication

2012 ◽  
Vol 39 (No. 3) ◽  
pp. 139-143 ◽  
Author(s):  
J. Svoboda ◽  
L. Svobodová-Leišová
Keyword(s):  
Czech Republic ◽  
Mosaic Virus ◽  
Short Communication ◽  
Alfalfa Mosaic Virus ◽  
Sources Of Resistance ◽  
Pepper Mild Mottle Virus ◽  
The Czech Republic ◽  
Tomato Spotted Wilt ◽  
Wilt Virus ◽  
Broad Bean Wilt Virus

A survey of viruses on capsicum plants in the Czech Republic was carried out in the years 2006&ndash;2010. Altogether, 375 leaf samples with symptoms suggesting viral infection were collected both from open fields and greenhouses. These samples were examined for the presence of Alfalfa mosaic virus (AMV), Broad bean wilt virus-1 (BBWV-1), Cucumber mosaic virus (CMV), Pepper mild mottle virus (PMMoV), Potato virus Y (PVY), Tobacco mosaic virus (TMV) and Tomato spotted wilt virus (TSWV) by ELISA. Viruses detected in the samples were AMV, BBWV-1, CMV and PVY. The most prevalent were CMV and PVY which were present in 24 and 29% of tested samples, respectively. In some cases a complex infection of two viruses was detected. Gene sources of resistance against CMV and PVY are mentioned. The relation of virus occurrence on aphid incidence is discussed.

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