scholarly journals First Report of Cucumber mosaic virus in Eryngium yuccifolium (Rattlesnake Master) in Ohio

Plant Disease ◽  
2004 ◽  
Vol 88 (12) ◽  
pp. 1384-1384 ◽  
Author(s):  
K. R. Whitten ◽  
S. G. P. Nameth
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Negative Control ◽  
Ohio State University ◽  
Enzyme Linked Immunosorbent Assay ◽  
State University ◽  
Double Stranded Rna ◽  
First Report ◽  
Random Samples ◽  
The Ohio State University

Rattlesnake master (Eryngium yuccifolium) is a wildflower that is native to Ohio. In recent years, native wildflowers have become very popular with home gardeners, and conservationists have begun to reestablish these plants in their native ranges. As native perennial wildflowers become more common, it is important to determine if they might serve as possible perennial reservoirs of viruses. A plot of 20 species native to Ohio was established on the Waterman Agricultural and Natural Resources Laboratory, The Ohio State University, Columbus, Ohio. In the summers of 1999 and 2001, random samples were collected from established plantings. Some sampled plants did not show symptoms of virus infection; however, all samples of E. yuccifolium appeared chlorotic, slightly mottled, and stunted. Using double-stranded RNA (dsRNA) analysis (1), these plants were assayed for viral infection. dsRNA profiles obtained from symptomatic E. yuccifolium resembled that of Cucumber mosaic virus (CMV). These results were confirmed with an enzyme-linked immunosorbent assay (ELISA; Agdia Inc., Elkhart, IN) for CMV. dsRNA-containing samples of E. yuccifolium produced ELISA absorbance values (A405) of 0.231 to 0.713 when compared with the negative control. All 14 samples of E. yuccifolium tested positive for CMV. To our knowledge, this is the first report of CMV in E. yuccifolium, which should serve as the basis for a more extensive survey, since CMV can potentially infect a wide variety of ornamental and nonornamental hosts. Reference: (1) R. Valverde et al. Plant Dis. 74:255, 1990.

scholarly journals Cucumber Mosaic Virus Associated with Leaf Malformation and Stunting in Adiantum pedatum

Plant Disease ◽  
1997 ◽  
Vol 81 (11) ◽  
pp. 1333-1333 ◽  
Author(s):  
S. T. Nameth ◽  
J. Steininger
Keyword(s):  
Virus Infection ◽  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Ohio State University ◽  
Enzyme Linked Immunosorbent Assay ◽  
State University ◽  
Double Stranded Rna ◽  
Symptomatic Tissue ◽  
The Ohio State University ◽  
General Appearance

Adiantum pedatum (maidenhair fern) is a fern commonly used in landscapes and interiorscapes for its attractive foliage. A. pedatum is quite hardy and under conditions of good soil fertility it can become highly intrusive if left unchecked. Maidenhair fern showing symptoms associated with possible virus infection were collected from the Chadwick Arboretum on the campus of The Ohio State University, Columbus. The leaves of the affected plants were slightly malformed such that the pinnules were arranged irregularly at the pinna. The sequence of the pinnae on the rachis and the general appearance of the rachis were distorted. Overall, the symptoms observed were not as severe as those described by Nienhaus et al. on other species of ferns (1). Viral-associated double-stranded RNA (dsRNA) analysis was used to analyze tissue from symptomatic and asymptomatic plants for evidence of virus infection. Results of dsRNA analysis gave evidence of a possible cucumovirus. There was no evidence of dsRNA in the asymptomatic tissue. Symptomatic tissue was subsequently tested for cucumber mosaic virus (CMV) with a direct antibody sandwich, enzyme-linked immunosorbent assay (ELISA). ELISA results were positive for CMV in symptomatic tissue and negative for CMV in asymptomatic tissue. This is the first report of a virus associated with a disease in A. pedatum. Reference: (1) F. Nienhaus et al. Z. Pflanzenkrankh. Pflanzenschutz 9:533, 1974.

scholarly journals First Report of Cucumber mosaic virus Subgroup II Infecting Lycopersicon esculentum in India

Plant Disease ◽  
2006 ◽  
Vol 90 (11) ◽  
pp. 1457-1457 ◽  
Author(s):  
N. Sudhakar ◽  
D. Nagendra-Prasad ◽  
N. Mohan ◽  
K. Murugesan
Keyword(s):  
Coat Protein ◽  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Tamil Nadu ◽  
Indian Subcontinent ◽  
Enzyme Linked Immunosorbent Assay ◽  
Infected Leaf ◽  
Polymorphism Analysis ◽  
First Report ◽  
Subgroup Ii

During a survey in January 2006 near Salem in Tamil Nadu (south India), Cucumber mosaic virus was observed infecting tomatoes with an incidence of more than 70%. Plants exhibiting severe mosaic, leaf puckering, and stunted growth were collected, and the virus was identified using diagnostic hosts, evaluation of physical properties of the virus, compound enzyme-linked immunosorbent assay (ELISA) (ELISA Lab, Washington State University, Prosser), reverse-transcription polymerase chain reaction (RT-PCR), and restriction fragment length polymorphism analysis (DSMZ, S. Winter, Germany). To determine the specific CMV subgroup, total RNA was extracted from 50 infected leaf samples using the RNeasy plant RNA isolation kit (Qiagen, Hilden, Germany) and tested for the presence of the complete CMV coat protein gene using specific primers as described by Rizos et al. (1). A fragment of the coat protein was amplified and subsequently digested with MspI to reveal a pattern of two fragments (336 and 538 bp), indicating CMV subgroup II. No evidence of mixed infection with CMV subgroup I was obtained when CMV isolates representing subgroups I (PV-0419) and II (PV-0420), available at the DSMZ Plant Virus Collection, were used as controls. Only CMV subgroup I has been found to predominantly infect tomato in the Indian subcontinent, although Verma et al. (2) identified CMV subgroup II infecting Pelargonium spp., an ornamental plant. To our knowledge, this is the first report of CMV subgroup II infecting tomato crops in India. References: (1) H. Rizos et al. J. Gen. Virol. 73:2099, 1992. (2) N. Verma et al. J. Biol. Sci. 31:47, 2006.

scholarly journals Cucumber Mosaic Virus, Tobacco Streak Virus, and Cucumber Mosaic Virus Satellite RNA Associated with Mosaic and Ringspot Symptoms in Ajuga reptans in Ohio

Plant Disease ◽  
1997 ◽  
Vol 81 (10) ◽  
pp. 1214-1214 ◽  
Author(s):  
J. R. Fisher ◽  
S. T. Nameth
Keyword(s):  
United States ◽  
Molecular Weight ◽  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
The United States ◽  
Negative Control ◽  
Enzyme Linked Immunosorbent Assay ◽  
Low Molecular Weight ◽  
Streak Virus ◽  
Ajuga Reptans

Creeping bugleweed (Ajuga reptans L.) is a perennial ornamental commonly grown as a ground cover in temperate climates. Commercial samples of the A. reptans cultivars Royalty, var. Atropurpurea Bronze, Bronze Beauty, and Burgundy Glow showing mosaic and ringspot symptoms were tested for the presence of virus infection by direct antibody sandwich enzyme-linked immunosorbent assay (ELISA) and viral-associated double-stranded (ds) RNA analysis. Cucumber mosaic cucumovirus (CMV) was detected by ELISA and dsRNA analysis in symptomatic samples of all cultivars tested. ELISA values were considered positive if the absorbance values were twice the negative control. Negative control values were established with asymptomatic tissue of the cv. Bronze Beauty. Tobacco streak ilarvirus (TSV) was detected only by ELISA in symptomatic samples of all cultivars except Royalty. No dsRNA suggestive of TSV was detected. Alfalfa mosaic virus (AMV) was detected by ELISA and dsRNA analysis in symptomatic samples of all cultivars tested except Royalty and var. Atropurpurea Bronze. dsRNA analysis also indicated the presence of a low molecular weight, possible satellite (sat) RNA associated with all symptomatic and asymptomatic Royalty and var. Atropurpurea Bronze plants tested. Northern (RNA) blot analysis with a digoxigenin-labeled full-length clone of the (S) CARNA-5 (-) CMV satRNA (ATCC no. 45124) confirmed that the low molecular weight RNA associated with the Royalty and var. Atropurpurea Bronze cultivars was indeed CMV satRNA. Only AMV has been previously reported in A. reptans in the United States (1). This is the first report of CMV and its satRNA, as well as TSV, in A. reptans in the United States. Reference: (1) W. T. Schroeder and R. Provvidenti. Plant Dis. Rep. 56:285, 1972.

Dalmatian Toadflax (Linaria dalmatica): New Host for Cucumber Mosaic Virus

2007 ◽  
Vol 21 (1) ◽  
pp. 41-44 ◽  
Author(s):  
Courtney L. Pariera Dinkins ◽  
Sue K. Brumfield ◽  
Robert K. D. Peterson ◽  
William E. Grey ◽  
Sharlene E. Sing
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Aphid Transmission ◽  
Negative Control ◽  
Enzyme Linked Immunosorbent Assay ◽  
New Host ◽  
Tobacco Plants ◽  
Linaria Dalmatica ◽  
Mechanical Methods ◽  
Dalmatian Toadflax

To date, there have been no reports of Dalmatian toadflax serving as a host for cucumber mosaic virus (CMV). Infestations of Dalmatian toadflax may serve as a reservoir of CMV, thereby facilitating aphid transmission of CMV to both agricultural crops and native plants. The goal of this study was to determine whether Dalmatian toadflax is a host for CMV. Dalmatian toadflax seedlings were randomly assigned to two treatments (18 replicates/treatment): no inoculation (control) and inoculation with CMV (Fast New York strain). The Dalmatian toadflax seedlings were inoculated by standard mechanical methods and tested for the presence of CMV using enzyme-linked immunosorbent assay (ELISA). Ten of the 18 CMV-inoculated toadflax plants tested positive for the virus; 6 of the 18 displayed systemic mosaic chlorosis and leaf curling. All control plants tested negative. Transmission electron microscopy obtained from CMV-positive plants confirmed the presence of CMV based on physical properties. To verify CMV infestation, tobacco plants were assigned to the following treatments (six replicates/treatment): no inoculation (control), CMV-negative (control) inoculation, and a CMV-positive inoculation. Plants were inoculated by standard methods. Five of the 6 tobacco plants treated with the CMV-positive inoculum tested positive for CMV using ELISA. All control plants tested negative for the virus.

scholarly journals First Report of Cucumber Mosaic Virus in Eryngium amethystinum, Canna spp., and Aquilegia hybrids in Ohio

Plant Disease ◽  
1997 ◽  
Vol 81 (11) ◽  
pp. 1331-1331 ◽  
Author(s):  
J. R. Fisher ◽  
M.-C. Sanchez-Cuevas ◽  
S. T. Nameth ◽  
V. L. Woods ◽  
C. W. Ellett
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Plant Viruses ◽  
Early Summer ◽  
Reservoir Host ◽  
Enzyme Linked Immunosorbent Assay ◽  
Herbaceous Plant ◽  
First Report ◽  
Severe Stunting ◽  
Banding Profile

Eryngium amethystinum (amethyst sea holly) is a herbaceous plant commonly grown as an ornamental perennial in U.S.D.A. hardiness zones 3 to 8. The plant thrives in dry areas with infertile soils and the flowers are often used in dried floral arrangements. Canna spp. (Canna), soft perennials (U.S.D.A. zone 9 and above), are becoming popular flowering plants because of their bright flowers and spectacular foliage. There are a variety of species that fall under the heading Canna spp., of which the most popular are C. glauca, C. indica, C. edulis, and C. iridiflora. Hybrids of Aquilegia (garden columbine), a hardy perennial (U.S.D.A. zones 3 to 9), flower in late spring through early summer. The genus is made up of a wide variety of cultivars. E. amethystinum exhibiting severe mosaic, yellowing, and stunting, along with Canna plants exhibiting severe stunting, chlorotic and distorted foliage, and mosaic, and garden columbine plants exhibiting stunting, leaf curl, chlorosis, and mosaic, collected from commercial plantings throughout the central Ohio area, were analyzed for the presence of virus infection with viral-associated, double-stranded RNA (dsRNA) analysis. dsRNA analysis resulted in a banding profile typical of that seen with members of the cucumovirus family of plant viruses. Plants positive for cucumovurus-like dsRNA were tested with a direct antibody sandwich enzyme-linked immunosorbent assay (ELISA). ELISA results confirmed the presence of cucumber mosaic virus (CMV) in all symptomatic plants tested. No evidence of dsRNA or CMV was found in any of the asymptomatic plants tested. Because all of these hosts are common in the perennial garden, they could serve as a reservoir host of CMV for other plants in the garden. This is the first report of CMV in E. amethystinum, Canna spp., and Aquilegia hybrids in Ohio.

scholarly journals First Report of a Cucumber mosaic virus-Associated Satellite RNA in Lobelia erinus

Plant Disease ◽  
2001 ◽  
Vol 85 (7) ◽  
pp. 802-802 ◽  
Author(s):  
S. G. P. Nameth ◽  
J. R. Fisher
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Leaf Tissue ◽  
Late Summer ◽  
Culture Collection ◽  
Northern Hybridization ◽  
Enzyme Linked Immunosorbent Assay ◽  
Satellite Rna ◽  
First Report ◽  
Dna Labeling

Lobelia (Lobelia erinus L.) is a common herbaceous annual used in flower beds and hanging baskets. The plant blooms from early to late summer. In the summer of 2000, Lobelia plants expressing virus-like symptoms were collected from a greenhouse-based production site in Ohio. Affected plants expressed a mild leaf mosaic and stunting. Viral-associated dsRNA was isolated from 7 g of symptomatic leaf tissue (1). Four dsRNAs were observed at 3.9, 3.0, 2.25, and 1.05 kb indicating the presence of a Cucumovirus. A fifth dsRNA at 0.75 kb also was observed, consistent with the presence of a satellite RNA. Enzyme-linked immunosorbent assay (ELISA) analysis (Agdia, Inc., Elkhart, IN) of symptomatic Lobelia tissue confirmed the presence of Cucumber mosaic virus (CMV). A (S)CARNA-5 (-) cDNA clone (American Type Culture Collection #45124) was labeled with digoxygenin (DIG) as per the manufacturer's instructions (Genius II DIG-DNA Labeling Kit, Boehringer Mannheim) and used as a diagnostic probe to detect this satellite RNA. Northern hybridization confirmed the identity of the satellite RNA (2). This is the first report of any satellite RNA associated with a virus infection in Lobelia and the first report of CMV in this host in Ohio. References: (1) J. R. Fisher and S. G. P. Nameth. HortScience 35:230–234, 2000. (2) R. A. Valverde et.al. Plant Dis. 74:255–258, 1990.

scholarly journals The First Report of Cucumber Mosaic Virus Infecting Water Chestnut in China

Plant Disease ◽  
2014 ◽  
Vol 98 (1) ◽  
pp. 164-164 ◽  
Author(s):  
J. Liu ◽  
Y. F. Wang ◽  
N. Hong ◽  
G. P. Wang ◽  
L. P. Wang
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Negative Control ◽  
Post Inoculation ◽  
Flanking Sequence ◽  
Nicotiana Glutinosa ◽  
First Report ◽  
Water Chestnut ◽  
Pcr Product ◽  
Commercial Kits

Water chestnut (Eleocharis dulcis), which is cultivated worldwide today, first originated in India and China. It is a popular seasonal aquatic vegetable valuable to people for its sweet crisp taste and rich nutrition. In October 2012, field-grown water chestnut seedlings (E. dulcis) showing mosaic, chlorotic, dwarfing, and malformed symptoms were observed in Fanggaoping Town, Tuanfeng County, Hubei Province, China. Sap from leaf-like stems of two symptomatic seedlings (BQ6 and BQ7) were mechanically inoculated onto Nicotiana glutinosa plants using 0.01 M phosphate buffer (pH 7.4) to investigate whether viral etiology was responsible for the disease. Typical symptoms of chlorosis and systemic mosaic similar to that inflicted by Cucumber mosaic virus (CMV) were observed on inoculated N. glutinosa leaves 13 days post inoculation, whereas mock inoculated seedlings remained symptomless. Three naturally field-grown symptomatic water chestnut and the inoculated N. glutinosa seedlings, together with a healthy water chestnut plant as negative control, were sampled. Double-antibody sandwich (DAS)-ELISA with antisera against CMV using commercial kits (Agdia, Elkhart, IN) was carried out to detect and confirm the presence of CMV. The symptomic water chestnut and inoculated N. glutinosa seedlings tested positive for CMV. Total RNAs were extracted using the SDS column isolation method from leaves of the inoculated N. glutinosa and stems of 13 field-grown symptomatic water chestnuts. The extracted RNAs were subjected to reverse transcription. The first-round PCR was carried out using the obtained cDNAs as template with the CMV specific primer set CMV-3F (5′-GCGATGYCGTGTTGAGAAG-3′) and CMV-3R (5′-TTTAGCCGTAAGCTGGATGGA-3′) targeting a 983-bp fragment covering 657 nt of the whole CP and partial flanking sequence within RNA3 referred as ‘Fny’ strain in GenBank (Accession No. D10538). The resulting amplicons were diluted 1:20 and further amplified with the nested-primer set CMV-P1 (5′-ATGGACAAATCTGAATCAACC-3′) and CMV-P2 (5′-TAAGCTGGATGGACAACCCGT-3′) targeting a fragment of 777 bp corresponding to the complete CP followed by part of 3′-UTRs of RNA3 (1). The amplicons of the expected size of ~777-bp were consistently amplified from 13 naturally infected water chestnuts and inoculated N. glutinosa. The PCR product derived from BQ6 isolate was cloned and three clones sequenced in both directions. The sequence (GenBank Accession No. KF268463) was analyzed by MEGA5 software (3). Sequence comparison of the complete CP gene of BQ6 isolate showed 98% nt and 99% amino acid (aa) identity with CMV isolate RP6 from South Korea (GenBank Accession No. KC527735) in subgroup I and had low similarities of 76% nt and 80% aa to that of CMV isolate infecting Trifolium from Hungary (GenBank Accession No. L15336) belonging to subgroup II of CMV. Phylogenetic analysis showed BQ6 isolate was more closely related to the isolates belonging to IB subgroup of CMV (GenBank Accession Nos. EF153739, DQ302715, and KC576805) (2). To our knowledge, this is the first report of CMV infecting water chestnut (E. dulcis) in China. CMV infection may pose a significant threat to water chestnut production. This result provide information to the producer that the CMV-free seedlings should be chosen for cultivation of water chestnut. References: (1) P. Palukaifis et al. Adv. Virus Res. 41:281, 1992. (2) S. K. Raj et al. Plant Dis. 92:171, 2008. (3) K. Tamura et al. Mol. Biol. Evol. 28:2731, 2011.

scholarly journals First Report of Cucumber mosaic virus in Garlic Mustard in Ohio

Plant Disease ◽  
2000 ◽  
Vol 84 (9) ◽  
pp. 1047-1047 ◽  
Author(s):  
M. J. Boehm ◽  
S. T. Nameth
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Plant Species ◽  
Enzyme Linked Immunosorbent Assay ◽  
Garlic Mustard ◽  
Weed Species ◽  
Alternate Host ◽  
First Report ◽  
Molecular Weights ◽  
Symptomatic Tissue

Garlic mustard (Alliaria officinalis) is a common weed species associated with woodland borders, hedge rows, and suburban gardens. Garlic mustard plants expressing foliar symptoms of leaf mosaic and vein banding were collected from Franklin and Cuyahoga counties in Ohio. Analysis of symptomatic tissue using viral-associated double-stranded RNA (dsRNA) analysis on 5% polyacrylamide gels and stained with ethidium bromide resulted in the production of a banding profile (four dsRNA bands with molecular weights of 2.6, 2.0, 1.5, and 0.7 × 106 daltons) similar to that of Cucumber mosaic virus (CMV) (1). Symptomatic tissue suspected of being infected with CMV was analyzed with an indirect enzyme-linked immunosorbent assay (iELISA) employing commercially produced antiserum (Agdia Inc.) against the common strain of CMV antiserum confirmed the presence of CMV. Nonsymptomatic tissue reacted negatively to CMV. This is the first report of CMV in garlic mustard in Ohio. Due to the extensive range of this weed and the wide host range of CMV in ornamental and food-plant species, garlic mustard could serve as an alternate host for CMV in many commercially important plant species. Reference: (1) T. J. Morris et al. Plant Mol. Biol. Rep. 1:27–30, 1983.

Identification of Three Distinct Classes of Satellite RNAs Associated With Two Cucumber mosaic virus Serotypes from the Ornamental Groundcover Vinca minor

2012 ◽  
Vol 13 (1) ◽  
pp. 18 ◽  
Author(s):  
John R. Fisher
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
The United States ◽  
Enzyme Linked Immunosorbent Assay ◽  
Rt Pcr ◽  
Pcr Cloning ◽  
Cloning And Sequencing ◽  
First Report ◽  
Vinca Minor ◽  
Satellite Rnas

Cucumber mosaic virus (CMV) is a cosmopolitan virus which may also have small satellite RNAs (satRNA) associated with it affecting symptom development. Vinca minor (periwinkle) plants exhibiting subtle mosaic symptoms tested positive for CMV by enzyme linked immunosorbent assay (ELISA). Double-stranded ribonucleic acid (dsRNA) analysis of CMV-Vinca field isolates in Nicotiana tabacum ‘Glurk’ suggested two sizes of putative satRNA associated with CMV. Immunocapture RT-PCR, cloning, and sequencing of the movement protein, coat protein, and satRNAs demonstrated serogroup 1A and serogroup 2 CMV helper strains and three distinct classes of satRNAs of four sizes. Further, two classes of satRNAs could be distinguished by their necrosis domains. Previously CMV was reported in V. minor in New Jersey. This is the first report of CMV in V. minor in Ohio and the first report of satRNA associated with CMV in V. minor in the United States. Accepted for publication 1 February 2012. Published 12 April 2012.

scholarly journals Inhibitory Effect of Chitosan and Phosphate Cross-linked Chitosan against Cucumber Mosaic Virus and Pepper Mild Mottle Virus

2021 ◽  
Vol 37 (6) ◽  
pp. 632-640
Author(s):  
Venkata Subba Reddy Gangireddygari ◽  
Bong Nam Chung ◽  
In-Sook Cho ◽  
Ju-Yeon Yoon
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Economic Loss ◽  
Crop Productivity ◽  
Inhibitory Effect ◽  
Negative Control ◽  
Cysteine Protease Inhibitor ◽  
Mottle Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Pepper Mild Mottle Virus

Cucumber mosaic virus (CMV) and Pepper mild mottle virus (PMMoV) causes severe economic loss in crop productivity of both agriculture and horticulture crops in Korea. The previous surveys showed that naturally available biopolymer material – chitosan (CS), which is from shrimp cells, reduced CMV accumulation on pepper. To improve the antiviral activity of CS, it was synthesized to form phosphate cross-linked chitosan (PCS) and compared with the original CS. Initially, the activity of CS and PCS (0.01%, 0.05%, and 0.1% concentration) compound against PMMoV infection and replication was tested using a half-leaf assay on Nicotiana glutinosa leaves. The total number of local lesions represented on a leaf of N. glutinosa were counted and analyzed with phosphate buffer treated leaves as a negative control. The leaves treated with a 0.1% concentration of CS or PCS compounds exhibited an inhibition effect by 40-75% compared with the control leaves. The same treatment significantly reduced about 40% CMV accumulation measured by double antibody sandwich enzyme-linked immunosorbent assay and increased the relative expression levels of the NPR1, PR-1, cysteine protease inhibitor gene, LOX, PAL, SRC2, CRF3 and ERF4 genes analyzed by quantitative reverse transcriptase-polymerase chain reaction, in chili pepper plants.

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