scholarly journals Isolation and Partial Nucleic Acid Characterization of a New Isolate of Potato virus V with Distinct Biological and Serological Properties

Plant Disease ◽  
2004 ◽  
Vol 88 (4) ◽  
pp. 368-372 ◽  
Author(s):  
P. J. Shiel ◽  
L. Miller ◽  
S. A. Slack ◽  
P. H. Berger
Keyword(s):  
Potato Virus ◽  
The United States ◽  
In Vitro Cultures ◽  
The World ◽  
Distinct Strain ◽  
Pathogen Screening ◽  
Polyclonal Antisera ◽  
The Common

In vitro cultures of potato (Solanum tuberosum cv. Papa Amarillo) were imported into the United States and were subjected to routine pathogen screening for newly introduced varieties. These cultures indexed positive for several of the six viruses tested and thus were treated using standard methods for eliminating viruses in in vitro cultures. Although the therapy apparently freed the potato of the tested viruses, some of the cultures continued to exhibit mosaic symptoms when planted in the greenhouse. When examined by electron microscopy, these plants contained flexuous rods. Tested samples did not index positive with any potato virus-specific polyclonal antisera but reacted with the “universal” potyvirus monoclonal antibody. These results indicated that these samples were likely infected with an as yet undescribed potyvirus. Further investigation indicated that this virus is a new strain of Potato virus V (PVV) that is serologically distinct from the common strain. This is the first description of a distinct strain of PVV. This particular strain has diverged sufficiently from other isolates of PVV to no longer be detectable by some commonly used antisera, and therefore is a concern for the future restriction of PVV spread into regions of the world where it is not currently present.

Virulence and Genetic Characterization of Six Baculovirus Strains Isolated From Different Populations of Spodoptera Frugiperda (Lepidoptera: Noctuidae)

2021 ◽  
Author(s):  
Ingrid Zanella-Saenz ◽  
Elisabeth A. Herniou ◽  
Jorge E. Ibarra ◽  
Ma.Cristina Del Rincón-Castro ◽  
Ilse Alejandra Huerta-Arredondo
Keyword(s):  
Biological Control ◽  
Cell Cultures ◽  
Spodoptera Frugiperda ◽  
Fall Armyworm ◽  
The United States ◽  
Infection Mechanisms ◽  
Different Populations

Abstract Fall armyworm (FAW), Spodoptera frugiperda (Smith, 1797), is a polyphagous, voracious, and economically important agricultural pest. Biological control of FAW is a strategy that must be further explored. This study evaluated six baculovirus strains isolated from infected FAW larvae from Mexico, Argentina, Honduras, and the United States. Five alphabaculoviruses (SfNPV-An2, SfNPV-Arg, SfNPV-Fx, SfNPV-Ho and SfNPV-Sin) and one betabaculovirus (SfGV-RV), were tested against FAW larvae, showing a wide diversity of virulence levels among strains when their estimated LC50s were compared, being SfNPVArg, SfNPV-Ho and SfNPV-Fx more virulent than SfNPV-An 2 , SfNPV-Sin and SfGV-RV. To determine any virulence difference in vitro studies of these isolates, Sf9 cell cultures were used. Interestingly, only ODVs from four of the test SfNPV strains showed infectivity on Sf9 cell cultures, and some differences in virulence were observed. Genomic restriction analyses and partial sequences of lef-8, lef-9 , and polh/granulin genes showed little variability among alphabaculoviruses, both, among them and with previously reported sequences. However, sequences from SfGV-RV were closer to previously reported sequences from the SfGVVG008 strain than the SfGV-Arg and SfGV-VG014 strains. The great difference in the in vivo virulence was not correlated with great similarity among the isolates. The characterization of these six baculoviruses isolates offers the basis for exploring their potential as biological control agents against S. frugiperda, as well the initial studies on their specific infection mechanisms, evolution, and ecology.

scholarly journals Characterization of Pythium Species Collected from a Multiple Time-Point Sampling of Cucurbits in South Carolina

Plant Disease ◽  
2020 ◽  
Vol 104 (11) ◽  
pp. 2832-2842
Author(s):  
Sean M. Toporek ◽  
Anthony P. Keinath
Keyword(s):  
South Carolina ◽  
The United States ◽  
Common Species ◽  
Multiple Time ◽  
Pythium Species ◽  
Multiple Time Point ◽  
Mitochondrial Cytochrome Oxidase ◽  
Time Point

Species of Pythium cause root and stem rot in cucurbits, but no formal surveys have been conducted in the United States to identify which species are responsible. The cucurbit hosts bottle gourd, cucumber, Hubbard squash, and watermelon were transplanted in May, July, September, and November into sentinel plots in four and five different fields in 2017 and 2018, respectively, in South Carolina. Eight of the nine fields were replanted in March 2019. Isolates (600) were collected and identified by sequencing DNA of the mitochondrial cytochrome oxidase I region. The four most common species were P. spinosum (45.6% of all isolates), P. myriotylum (20.0%), P. irregulare (15.3%), and P. aphanidermatum (12.8%). P. myriotylum and P. aphanidermatum were predominantly isolated in May, July, and September, whereas P. spinosum and P. irregulare were predominantly isolated in November and March. Isolates of P. ultimum, P. irregulare, and P. spinosum were more virulent than isolates of P. myriotylum and P. aphanidermatum at 25°C. Representative isolates were screened in vitro for sensitivity to three fungicides: mefenoxam, propamocarb, and oxathiapiprolin. All isolates were sensitive to mefenoxam and propamocarb, but these same isolates were insensitive to oxathiapiprolin, except those classified taxonomically in Pythium clade I.

The Unity of the West and Free Trade: A Report From Washington

Worldview ◽  
1970 ◽  
Vol 13 (6) ◽  
pp. 14-16
Author(s):  
Lionel Gelber
Keyword(s):  
United States ◽  
Free Trade ◽  
Western Europe ◽  
The United States ◽  
Common Market ◽  
The West ◽  
The World ◽  
The Future ◽  
The Common ◽  
De Gaulle

When the United States fostered the recovery and underwrote the security of Western Europe she had more than sentiment to impel her. That salient zone is a pivotal sector of the world balance, and while she may station fewer of her own troops upon its soil, she can entertain no total disengagement from it. But there is another West European item, the future of the Common Market, which calls for a fresh American scrutiny. The West will be better off if Western Europe acquires more of an ability to stand on its own feet. Gaullism, however, revealed a less modest goal, one that was not confined to France and did not vanish with the departure of General de Gaulle. On the contrary, it may have gained new leverage from his downfall.

scholarly journals Characterization of Vancomycin-Resistant Enterococcus faecium Isolates from the United States and Their Susceptibility In Vitro to Dalfopristin-Quinupristin

1998 ◽  
Vol 42 (5) ◽  
pp. 1088-1092 ◽  
Author(s):  
G. M. Eliopoulos ◽  
C. B. Wennersten ◽  
H. S. Gold ◽  
T. Schülin ◽  
M. Souli ◽  
...  
Keyword(s):  
United States ◽  
Enterococcus Faecium ◽  
Multiple Drug Resistance ◽  
The United States ◽  
Multiple Drug ◽  
Data Set ◽  
Vancomycin Resistant ◽  
High Level

ABSTRACT In the course of clinical studies with the investigational streptogramin antimicrobial dalfopristin-quinupristin, isolates of vancomycin-resistant Enterococcus faecium were referred to our laboratory from across the United States. Seventy-two percent of the strains were of the VanA type, phenotypically and genotypically, while 28% were of the VanB type. High-level resistance to streptomycin or gentamicin was observed in 86 and 81%, respectively, of the VanA strains but in only 69 and 66%, respectively, of the VanB strains. These enterococci were resistant to ampicillin (MIC for 50% of the isolates tested [MIC50] and MIC90, 128 and 256 μg/ml, respectively) and to the other approved agents tested, with the exception of chloramphenicol (MIC90, 8 μg/ml) and novobiocin (MIC90, 1 μg/ml). Considering all of the isolates submitted, dalfopristin-quinupristin inhibited 86.4% of them at concentrations of ≤1 μg/ml and 95.1% of them at ≤2 μg/ml. However, for the data set comprised of only the first isolate submitted for each patient, 94.3% of the strains were inhibited at concentrations of ≤1 μg/ml and 98.9% were inhibited at concentrations of ≤2 μg/ml. Multiple drug resistance was very common among these isolates of vancomycin-resistant E. faecium, while dalfopristin-quinupristin inhibited the majority at concentrations that are likely to be clinically relevant.

The Oneness-Trinitarian Pentecostal Dialogue: Exploring the Diversity of Apostolic Faith

2010 ◽  
Vol 103 (3) ◽  
pp. 329-349
Author(s):  
Frank D. Macchia
Keyword(s):  
Subsequent Development ◽  
The United States ◽  
World Council Of Churches ◽  
Study Program ◽  
Starting Point ◽  
The World ◽  
World Council ◽  
The Common ◽  
Significant Measure ◽  
Nicene Creed

The Nicene Creed and the subsequent development of Trinitarian orthodoxy have been regarded by many as essential to the apostolic faith of the churches. For example, the Nicene-Constantinopolitan Creed without the filioque clause was made the starting point of the World Council of Churches' Commission on Faith and Order study program entitled, “Towards the Common Expression of the Apostolic Faith Today.” Not so well known, however, is the existence of a growing movement of Pentecostal Christians globally that seeks to preserve the apostolic faith of the churches in significant measure by rejecting the Nicene-Constantinopolitan Creed along with the Trinitarian dogma that historically it had supported. Commonly called Oneness or Apostolic Pentecostals, they are estimated to have from 14 to over 17 million followers globally and growing rapidly in Mexico, China, and the United States.1

Molecular Characterization of Potato leafroll virus, Potato virus A, and Potato virus X Isolates from Potatoes in Alaskan Cities and Villages

2011 ◽  
Vol 12 (1) ◽  
pp. 39 ◽  
Author(s):  
Nancy L. Robertson ◽  
Jeffrey Smeenk ◽  
Jodie M. Anderson
Keyword(s):  
Molecular Characterization ◽  
Potato Virus ◽  
Potato Virus X ◽  
Potato Leafroll Virus ◽  
First Report ◽  
Potato Virus A ◽  
Potato Viruses ◽  
The World ◽  
Leafroll Virus

Although all three viruses are commonly found in potatoes throughout the world, this is the first report of potato viruses from Alaska to be sequenced and molecularly analyzed for comparisons with known viruses. Accepted for publication 17 January 2011. Published 9 February 2011.

Characterization of cytotoxic cells generated from in vitro cultures of murine bone marrow cells

1985 ◽  
Vol 92 (1) ◽  
pp. 1-13 ◽  
Author(s):  
Gary R. Klimpel ◽  
Marcella Sarzotti ◽  
Victor E. Reyes ◽  
Kathleen D. Klimpel
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
In Vitro Cultures ◽  
Murine Bone Marrow ◽  
Cytotoxic Cells ◽  
Marrow Cells

scholarly journals Characterization of Anaplasma marginale Isolated from North American Bison

2003 ◽  
Vol 69 (8) ◽  
pp. 5001-5005 ◽  
Author(s):  
José de la Fuente ◽  
Elizabeth J. Golsteyn Thomas ◽  
Ronald A. Van Den Bussche ◽  
Robert G. Hamilton ◽  
Elaine E. Tanaka ◽  
...  
Keyword(s):  
New World ◽  
Surface Protein ◽  
Anaplasma Marginale ◽  
The United States ◽  
Bison Bison ◽  
Infected Cattle ◽  
Major Surface Protein ◽  
The World ◽  
Major Surface

ABSTRACT Anaplasma marginale (Rickettsiales: Anaplasmataceae), a tick-borne pathogen of cattle, is endemic in tropical and subtropical regions of the world. Although serologic tests have identified American bison, Bison bison, as being infected with A. marginale, the present study was undertaken to confirm A. marginale infection and to characterize isolates obtained from naturally infected bison in the United States and Canada. Major surface protein (MSP1a and MSP4) sequences of bison isolates were characterized in comparison with New World cattle isolates. Blood from one U.S. bison was inoculated into a susceptible, splenectomized calf, which developed acute anaplasmosis, demonstrating infectivity of this A. marginale bison isolate for cattle. The results of this study showed that these A. marginale isolates obtained from bison were similar to ones from naturally infected cattle.

scholarly journals University vs. Society in a Period of Nation Building: The Hebrew University in Pre-State Israel

2007 ◽  
pp. 81-110
Author(s):  
Uri Cohen
Keyword(s):  
Political Leadership ◽  
Jewish People ◽  
The United States ◽  
It Service ◽  
The World ◽  
Hebrew University ◽  
Close Cooperation ◽  
The Common ◽  
The University ◽  
Important Building Block

This article examines the process by which the Hebrew University of Jerusalem was established, from 1925-1948, during the period of the British Mandate in Palestine. It finds that the World Zionist Organization was deeply involved in this project, viewing a university as an important building block in the creation of a Jewish nationality in the Land of Israel. But alongside this Zionist role, Jewish communities around the world, particularly in Europe and the United States, also played an important academic and financial role in the establishment of the Hebrew University. This study reveals that, in contrast to the common pattern of close cooperation that is characteristic of relations between academic institutions and cohering national elites, the Hebrew University consistently pursued an academic policy that generated acute tension vis-à-vis the political leadership in Jewish Palestine. The University deliberately nurtured an elitist policy while rejecting attempts to make it service the general public needs, as defined by the local political leadership. Rather, the University saw its central role as being a “university for the dispersed nation,” that is, a university for all of the Jewish people.

Pluralism in Religion and American Education

2018 ◽  
Author(s):  
Diana L. Eck ◽  
Brendan Randall
Keyword(s):  
United States ◽  
Civic Education ◽  
Religious Diversity ◽  
The United States ◽  
First Century ◽  
American Education ◽  
The Past ◽  
The World ◽  
Established Fact ◽  
The Common

The United States is among the most religiously diverse countries in the world. Although such diversity is not a new phenomenon, its degree and visibility have increased dramatically in the past fifty years, reigniting the debate over a fundamental civic question: What is the common identity that binds us together? How we respond to religious diversity in the context of education has enormous implications for our democratic society. To the extent that previous frameworks such as exclusion or assimilation ever were desirable or effective, they no longer are. Increased religious diversity is an established fact and growing trend. The United States needs a more inclusive and robust civic framework for religious diversity in the twenty-first century—pluralism—and this framework should be an essential component of civic education.

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