scholarly journals Biological and Molecular Analyses of the Acibenzolar-S-Methyl-Induced Systemic Acquired Resistance in Flue-Cured Tobacco Against Tomato spotted wilt virus

2008 ◽  
Vol 98 (2) ◽  
pp. 196-204 ◽  
Author(s):  
B. Mandal ◽  
S. Mandal ◽  
A. S. Csinos ◽  
N. Martinez ◽  
A. K. Culbreath ◽  
...  
Keyword(s):  
Tomato Spotted Wilt Virus ◽  
Systemic Acquired Resistance ◽  
Field Experiments ◽  
Acquired Resistance ◽  
Systemic Infection ◽  
Inverse Correlation ◽  
Challenge Inoculation ◽  
Tomato Spotted Wilt ◽  
High Level ◽  
Wilt Virus

Tomato spotted wilt virus (TSWV) is an economically important virus of flue-cured tobacco. Activation of systemic acquired resistance (SAR) by acibenzolar-S-methyl (ASM) in flue-cured tobacco was studied under greenhouse conditions by challenge inoculation with a severe isolate of TSWV. ASM restricted virus replication and movement, and as a result reduced systemic infection. Activation of resistance was observed within 2 days after treatment with ASM and a high level of resistance was observed at 5 days onward. Expression of the pathogenesis-related (PR) protein gene, PR-3, and different classes of PR proteins such as PR-1, PR-3, and PR-5 were detected at 2 days post-ASM treatment which inversely correlated with the reduction in the number of local lesions caused by TSWV. Tobacco plants treated with increased quantities of ASM (0.25, 0.5, 1.0, 2.0, and 4.0 g a.i./7,000 plants) showed increased levels of SAR as indicated by the reduction of both local and systemic infections by TSWV. The highest level of resistance was at 4 g a.i., but this rate of ASM also caused phytotoxicity resulting in temporary foliar spotting and stunting of plants. An inverse correlation between the TSWV reduction and phytotoxicity was observed with the increase of ASM concentration. ASM at the rate of 1 to 2 g a.i./7,000 plants activated a high level of resistance and minimized the phytotoxicity. Use of gibberellic acid in combination with ASM reduced the stunting caused by ASM. Present findings together with previous field experiments demonstrate that ASM is a potential option for management of TSWV in flue-cured tobacco.

scholarly journals EVALUATION OF LYCOPERSICON GERMPLASM COLLECTION FOR RESISTANCE TO TOMATO SPOTTED WILT VIRUS

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1077b-1077
Author(s):  
S.J. Scott ◽  
M. Stevens ◽  
R.C. Gergerich
Keyword(s):  
Immunosorbent Assay ◽  
Tomato Spotted Wilt Virus ◽  
Germplasm Collection ◽  
Systemic Infection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Additional Species ◽  
Tomato Spotted Wilt ◽  
Lycopersicon Species ◽  
Wilt Virus

Seedlings of eight accessions of L. hirsutum and susceptible L. esculentum `VF Pink' controls were spray inoculated twice in the greenhouse with tomato spotted wilt virus (TSWV) Arkansas 85-9. Plants lacking symptoms were reinoculated, then evaluated for TSWV by enzyme-linked immunosorbent assay (ELISA). Controls were consistently infected; sixty noninfected L. hirsutum were propagated by cuttings and inoculated with TSWV isolates T2 (lettuce), G-87 (gloxinia), 87-34 (tomato) and a mixture of the four isolates. All selections became infected in at least one test, but systemic infection was often delayed. Additional wild Lycopersicon species and numbers of accessions evaluated for resistance to TSWV include L. cheesmanii (9), L. chmielewskii (17), L. hirsutum (24), L. hirsutum f. glabratum (17), L. parviflorum (4) and L. pennellii (44). No new sources of strong resistance have been identified yet. Evaluation of additional species and accessions is continuing.

Response of Eight Peanut (Arachis hypogaea L.) Cultivars to the Herbicide AC 263,222

1995 ◽  
Vol 22 (1) ◽  
pp. 76-80 ◽  
Author(s):  
John S. Richburg ◽  
John W. Wilcut ◽  
Albert K. Culbreath ◽  
Craig K. Kvien
Keyword(s):  
Tomato Spotted Wilt Virus ◽  
Field Experiments ◽  
Stem Rot ◽  
Lower Percentage ◽  
Tomato Spotted Wilt ◽  
Arachis Hypogaea L ◽  
Main Effect ◽  
Peanut Cultivars ◽  
Ac 263,222 ◽  
Wilt Virus

Abstract Field experiments conducted in 1992 and 1993 in Georgia evaluated the response of the peanut cultivars Florunner, Georgia Runner, Goldkist GK 7, NC 7, NC-V11, Southern Runner, Sunrunner, and AgraTech VC-1 to AC 263,222 applied early-postemergence (EPOST) at 71 g ha-1. Visual injury, which was less than 13%, was independent of cultivar, and injury was not evident on any cultivar midseason. AC 263,222 slightly reduced canopy width consistently on all cultivars. The only significant effect on percentage fancy pods, medium, No. 1, sound splits, damaged, and other kernels was the main effect of cultivar. There was a significant cultivar, AC 263,222, and cultivar-by-AC 263,222 effect on extra large kernels (ELK) and jumbo kernel percentages. Southern Runner and NC-V11 had significantly lower percentage jumbo and ELK, respectively when treated with AC 263,222. In addition, Florunner and Sunrunner also had numerically less percentage jumbo kernels. Reductions in percentages of ELK and jumbo were probably the result of AC 263,222-induced maturity delays. However, these reductions were not reflected in peanut yields. The occurence of late and early leaf spot, tomato spotted wilt virus (TSWV), and southern stem rot was independent of both cultivar and AC 263,222.

scholarly journals Natural Resources Resistance to Tomato Spotted Wilt Virus (TSWV) in Tomato (Solanum lycopersicum)

2021 ◽  
Vol 22 (20) ◽  
pp. 10978
Author(s):  
Shiming Qi ◽  
Shijie Zhang ◽  
Md. Monirul Islam ◽  
Ahmed H. El-Sappah ◽  
Fei Zhang ◽  
...  
Keyword(s):  
Solanum Lycopersicum ◽  
Resistance Genes ◽  
Theoretical Basis ◽  
Tomato Spotted Wilt Virus ◽  
Natural Resistance ◽  
High Concentration ◽  
Tomato Spotted Wilt ◽  
Global Spread ◽  
High Level ◽  
Wilt Virus

Tomato spotted wilt virus (TSWV) is one of the most destructive diseases affecting tomato (Solanum lycopersicum) cultivation and production worldwide. As defenses against TSWV, natural resistance genes have been identified in tomato, including Sw-1a, Sw-1b, sw-2, sw-3, sw-4, Sw-5, Sw-6, and Sw-7. However, only Sw-5 exhibits a high level of resistance to the TSWV. Thus, it has been cloned and widely used in the breeding of tomato with resistance to the disease. Due to the global spread of TSWV, resistance induced by Sw-5 decreases over time and can be overcome or broken by a high concentration of TSWV. How to utilize other resistance genes and identify novel resistance resources are key approaches for breeding tomato with resistance to TSWV. In this review, the characteristics of natural resistance genes, natural resistance resources, molecular markers for assisted selection, and methods for evaluating resistance to TSWV are summarized. The aim is to provide a theoretical basis for identifying, utilizing resistance genes, and developing tomato varieties that are resistant to TSWV.

scholarly journals Influence of Paraquat on Yield and Tomato Spotted Wilt Virus for Georgia-02C and Georgia-03L Peanut

2010 ◽  
Vol 37 (1) ◽  
pp. 39-43 ◽  
Author(s):  
R. S. Tubbs ◽  
E. P. Prostko ◽  
R. C. Kemerait ◽  
T. B. Brenneman ◽  
D. Q. Wann
Keyword(s):  
Tomato Spotted Wilt Virus ◽  
Field Experiments ◽  
Yield Reduction ◽  
Visible Injury ◽  
Growing Seasons ◽  
Tomato Spotted Wilt ◽  
Herbicide Treatments ◽  
Treatment Interaction ◽  
Peanut Production ◽  
Wilt Virus

Abstract Paraquat is a common herbicide used in peanut production; however, visible injury and reduced yield have been observed in some instances. Most research regarding paraquat injury on peanut has taken place on cultivars that are no longer available and were more susceptible to tomato spotted wilt virus (TSWV) than current cultivars. Field experiments were conducted over three growing seasons to determine the effect of paraquat on yield and TSWV incidence in two moderately TSWV-resistant cultivars (Georgia-02C and Georgia-03L). Paraquat and paraquat plus bentazon were evaluated against a non-treated control at four application timings [7, 14, 21, and 28 d after ground cracking (DAGC)]. There were no yield differences among herbicide treatments or application timings for Georgia-02C peanut, but there was a treatment interaction with Georgia-03L for yield. The majority of interaction comparisons showed no yield differences, but the non-treated control had higher yields than the herbicide treatments when significance did occur. Yields were similar for the 7 DAGC timing in all comparisons. In all instances when differences occurred for both cultivars, TSWV was higher in non-treated plots than where herbicides were applied. This data supports the use of paraquat in Georgia-02C and Georgia-03L peanut since there is minimal chance of yield reduction and may also reduce TSWV incidence; however, additional studies are required.

scholarly journals Molecular Co-Chaperone SGT1 Is Critical for Cell-to-Cell Movement and Systemic Infection of Tomato Spotted Wilt Virus in Nicotiana benthamiana

Viruses ◽  
2018 ◽  
Vol 10 (11) ◽  
pp. 647 ◽  
Author(s):  
Xin Qian ◽  
Qing Xiang ◽  
Tongqing Yang ◽  
Hongyu Ma ◽  
Xin Ding ◽  
...  
Keyword(s):  
Virus Infection ◽  
Nicotiana Benthamiana ◽  
Tomato Spotted Wilt Virus ◽  
Rna Virus ◽  
Cell Movement ◽  
Systemic Infection ◽  
Plant Viruses ◽  
Host Factors ◽  
Tomato Spotted Wilt ◽  
Wilt Virus

Tospovirus is a tripartite negative stranded RNA virus and is considered as one of the most devastating plant viruses. Successful virus infection in plant requires many host factors. To date, very few host factors have been identified as important in Tospovirus infection in plants. We reported earlier that NSm protein encoded by Tomato spotted wilt virus (TSWV), a type species of the genus Orthotospovirus, plays critical roles in viral cell-to-cell and long-distance movement. In this study, we determined that molecular co-chaperone NbSGT1 interacted with TSWV NSm in Nicotiana benthamiana. TSWV infection significantly upregulated the expression of NbSGT1 gene and transient overexpression of NbSGT1 in N. benthamiana leaves accelerated TSWV infection. In contrast, silencing the NbSGT1 gene expression using a virus-induced gene silencing (VIGS) approach strongly inhibited TSWV NSm cell-to-cell movement, as well as TSWV local and systemic infection in N. benthamiana plants. Furthermore, NbSGT1 was found to regulate the infection of both American and Euro/Asia type tospoviruses in N. benthamiana plant. Collectively, our findings presented in this paper and the results published previously indicated that molecular co-chaperone NbSGT1 plays important roles in modulating both positive stranded and tripartite negative stranded RNA virus infection in plants.

scholarly journals Characterization of the Roles of SGT1/RAR1, EDS1/NDR1, NPR1, and NRC/ADR1/NRG1 in Sw-5b-Mediated Resistance to Tomato Spotted Wilt Virus

Viruses ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 1447
Author(s):  
Zhengqiang Chen ◽  
Qian Wu ◽  
Cong Tong ◽  
Hongyu Chen ◽  
Dan Miao ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Tomato Spotted Wilt Virus ◽  
Tobacco Rattle Virus ◽  
Systemic Infection ◽  
Systemic Resistance ◽  
Central Component ◽  
Virus Induced Gene Silencing ◽  
Specific Domain ◽  
Tomato Spotted Wilt ◽  
Wilt Virus

The tomato Sw-5b gene confers resistance to tomato spotted wilt virus (TSWV) and encodes a nucleotide-binding leucine-rich repeat (NLR) protein with an N-terminal Solanaceae-specific domain (SD). Although our understanding of how Sw-5b recognizes the viral NSm elicitor has increased significantly, the process by which Sw-5b activates downstream defense signaling remains to be elucidated. In this study, we used a tobacco rattle virus (TRV)-based virus-induced gene silencing (VIGS) system to investigate the roles of the SGT1/RAR1, EDS1/NDR1, NPR1, and NRC/ADR1/NRG1 genes in the Sw-5b-mediated signaling pathway. We found that chaperone SGT1 was required for Sw-5b function, but co-chaperone RAR1 was not. Sw-5b-mediated immune signaling was independent of both EDS1 and NDR1. Silencing NPR1, which is a central component in SA signaling, did not result in TSWV systemic infection in Sw-5b-transgenic N. benthamiana plants. Helper NLR NRCs (NLRs required for cell death) were required for Sw-5b-mediated systemic resistance to TSWV infection. Suppression of NRC2/3/4 compromised the Sw-5b resistance. However, the helper NLRs ADR1 and NRG1 may not participate in the Sw-5b signaling pathway. Silencing ADR1, NRG1, or both genes did not affect Sw-5b-mediated resistance to TSWV. Our findings provide new insight into the requirement for conserved key components in Sw-5b-mediated signaling pathways.

scholarly journals Resistance Response of the Recently Discovered Species Nicotiana mutabilis to Potato virus Y (PVY) and Tomato spotted wilt virus (TSWV) Compared to Other Sources of Resistance

Agronomy ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1617
Author(s):  
Anna Depta ◽  
Teresa Doroszewska ◽  
Anna Czubacka
Keyword(s):  
Potato Virus ◽  
Tomato Spotted Wilt Virus ◽  
Potato Virus Y ◽  
Susceptibility Gene ◽  
Systemic Infection ◽  
Sources Of Resistance ◽  
Resistance Response ◽  
Tomato Spotted Wilt ◽  
Wilt Virus ◽  
Das Elisa

Nicotiana mutabilis is a recently discovered species within the genus Nicotiana. The aim of the present study was to evaluate its resistance to Potato virus Y (PVY) and Tomato spotted wilt virus (TSWV). Molecular analysis was performed to detect the Va gene determining susceptibility to PVY and the SCAR marker associated with resistance to TSWV. Resistance tests were carried out under greenhouse conditions through artificial inoculation with one TSWV and two PVY isolates. In order to confirm the presence of the viruses in plants, DAS-ELISA tests were performed using antibodies against PVY and TSWV. The results indicated the absence of the PVY susceptibility gene and the presence of the TSWV resistance gene in the genome of N. mutabilis. This species was considered tolerant to the two PVY isolates tested because, despite the positive DAS-ELISA results, the infected plants showed vein clearing and chlorotic spots but no vein necrosis. As a result of TSWV inoculation, N. mutabilis showed a hypersensitive response; however, after four months, 30% of the inoculated plants showed systemic infection. This species extends the genetic variation in the genus Nicotiana and, because of its tolerance to PVY and partial resistance to TSWV, it may be a potential source of resistance to these viruses.

scholarly journals Tomato spotted wilt virus Can Infect Resistant Tomato when Western Flower Thrips Inoculate Blossoms

Plant Disease ◽  
2017 ◽  
Vol 101 (9) ◽  
pp. 1666-1670 ◽  
Author(s):  
J. L. Houle ◽  
G. G. Kennedy
Keyword(s):  
Tomato Spotted Wilt Virus ◽  
Frankliniella Occidentalis ◽  
Field Experiments ◽  
Control Method ◽  
Western Flower Thrips ◽  
Effective Control ◽  
Flower Thrips ◽  
Tomato Spotted Wilt ◽  
Systemic Infections ◽  
Wilt Virus

Tomato spotted wilt is a major disease of crops worldwide. Resistant cultivars carrying the Sw-5 allele for resistance to tomato spotted wilt disease (TSW) provide the most effective control method in tomato (Solanum lycopersicum). However, infections of fruit on Sw-5+ tomato plants suggest the virus resistance may not be fully expressed in blossoms or developing fruit. The objective of this study was to determine if the thrips vector, the western flower thrips (Frankliniella occidentalis), can transmit non-resistance breaking Tomato spotted wilt virus (TSWV) isolates when confined to blossoms on plants with and without the Sw-5 resistance allele. Twenty-one percent of 33 Sw-5+ plants inoculated by adult thrips feeding on blossom clusters or small fruit developed infections in the reproductive tissue, whereas 68% of 25 Sw-5– plants developed infections. Systemic infections also occurred following inoculation of blossoms in host genotypes with and without Sw-5. These results were further supported by field experiments that showed high proportions of infected fruit as well as a limited infection of foliage on the same stem as the infected fruit in Sw-5+ plants when F. occidentalis were abundant in blossoms. These findings help to explain observations of abundant late season infections of Sw-5 cultivars in commercial plantings and suggest that management of F. occidentalis infestations during the bloom period may be important for effective management of TSWV in susceptible tomato cultivars as well as cultivars expressing the Sw-5 allele for TSW resistance.

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