scholarly journals Factors affecting HPLC analysis of vitamin K in serum, food and dietary supplements

2011 ◽  
Vol 25 (S1) ◽  
Author(s):  
Neal E Craft ◽  
Heasoon Park
Keyword(s):  
Dietary Supplements ◽  
Vitamin K ◽  
Hplc Analysis ◽  
Factors Affecting

P2753Anticoagulation control and all-cause death in patients with operated valvular heart disease with and without atrial fibrillation receiving vitamin K antagonists

2019 ◽  
Vol 40 (Supplement_1) ◽  
Author(s):  
H Zulkifly ◽  
D Pastori ◽  
G Y H Lip ◽  
D Lane
Keyword(s):  
Atrial Fibrillation ◽  
Heart Disease ◽  
Vitamin K ◽  
Therapeutic Range ◽  
Valvular Heart Disease ◽  
Prosthetic Valve ◽  
Factors Affecting ◽  
Anticoagulation Control ◽  
Bleeding History

Abstract Introduction Good quality of anticoagulation in patients with valvular heart disease (VHD) is needed to reduce ischaemic complications. There is limited evidence on factors affecting anticoagulation control in patients implanted with mechanical or tissue prosthetic valve(s). Objective To examine quality, factors affecting anticoagulation control and all-cause death in VHD patients with and without atrial fibrillation (AF) receiving a vitamin K antagonist (VKA) oral anticoagulant. The relationship between INR control with all-cause death and ≥1 adverse clinical events (ACE) [thromboembolism, bleeding, cardiovascular hospitalisation and all-cause death] were explored. Methods Anticoagulation control of 456 VHD patients [164 (36%) with AF and 290 (64%) without AF] referred to a hospital-based anticoagulation clinic were assessed retrospectively by time in therapeutic range (TTR) (Rosendaal) and percentage of INRs in range (PINRR) for a median of (IQR) 6.2 years (3.3–8.5). VHD was defined by the presence of mechanical or tissue prosthetic valve at either the mitral or aortic site or both. Results Mean (SD) age 51 (14.7), 64.5% male, mean (SD) CHA2DS2-VASc score 2.0 (1.4), 96.1% mechanical prosthesis and 64% aortic valve replacement. VHD patients with AF had lower mean TTR and PINRR, lower proportion of optimal TTR (i.e.≥70%) despite similar number of INR tests compared to VHD patients without AF [Table 1]. Predictors of poor TTR on multivariate logistic regression analysis were female sex, AF and anaemia/bleeding history. Significantly higher proportions of VHD patients with AF died [Table 1]. More deaths (13.1% vs. 4.1%; p=0.011) and ≥1 ACE (42.7% vs. 27.6%; p=0.006) were seen in VHD patients with TTR <70% vs. TTR≥70%, respectively. Table 1 N (%) Total (N=456) AF (N=164) No AF (N=290) p-value Mean (SD) TTR 58.5 (14.6) 55.7 (14.2) 60.1 (14.6) 0.002 TTR ≥70% 98 (21.5) 23 (14.0) 75 (25.7) 0.004 Mean (SD) PINRR 50.1 (13.8) 47.4 (13.5) 51.6 (13.7) 0.002 Mean (SD) INR tests 96.2 (55.3) 100.7 (58.8) 93.7 (53.1) 0.19 All-cause death 51 (11.2) 34 (20.7) 17 (5.8) <0.001 AF: Atrial fibrillation; IQR: interquartile range; PINRR: percentage of INRs in range; SD: standard deviation; TTR: Time in therapeutic range. Conclusion The quality of anticoagulation in VHD patients with AF was low. The presence of AF, anaemia/bleeding history and female sex independently predicted poor TTR. All-cause death was more common in VHD patients with AF and poor TTR. Closer INR monitoring is needed especially in VHD patients with AF to improve anticoagulation control and prevent adverse clinical outcomes. Acknowledgement/Funding Kementerian Pendidikan Malaysia and Universiti Teknologi MARA for PhD study but not directly for work under consideration

scholarly journals FACTORS AFFECTING THE RECOVERY YIELD AND THE PURITY OF N-ACETYL GLUCOSAMINE FROM CHITIN HYDROLYSATE Dang

2018 ◽  
Vol 54 (4A) ◽  
pp. 23
Author(s):  
Dang Thi Huong
Keyword(s):  
Hplc Analysis ◽  
Optimal Concentration ◽  
Absolute Ethanol ◽  
Factors Affecting ◽  
Recovery Yield ◽  
Optimal Ratio ◽  
Optimal Value ◽  
Lower Recovery

In this study, different ratio of ethanol to hydrolysates obtained by hydrolyzing chitin by chitinase as well as concentration of hydrolysates was investigated to find the optimal value. The result showed that the optimal ratio of absolute ethanol to hydrolysates was 9:1. The further increase of this ratio up to 11:1 resulted only in insignificant increase of N-acetylglucosamine (NAG) recovery yield from 74.47 % to 75.39 % and NAG purity from 93.25 % to 93.44 %. Concentration of hydrolysate also affected the NAG recovery yield and NAG purity. The impurity of crystallization by cooling down the hydrolysates appeared only when hydrolysate was concentrated at a certain level. The mass of crystallization impurity was increased sharply from 0 to 0.082 mg/ml when hydrolysate’s concentration was increased significantly from 1 to 7.9 fold, then slightly from 0.082 to 0.093 mg/ml as its concentration further increased up to 15.7 fold. Higher concentration of hydrolysate resulted in lower recovery yield but purer product. According to HPLC analysis, 93.25 % of NAG purity with recovery yield 74.47% was obtained at optimal ethanol/hydrolysates ratio and optimal concentration of hydrolysate.

scholarly journals Determination of Vitamins K1, K2 MK-4, MK-7, MK-9 and D3 in Pharmaceutical Products and Dietary Supplements by TLC-Densitometry

Processes ◽  
2020 ◽  
Vol 8 (7) ◽  
pp. 870
Author(s):  
Urszula Hubicka ◽  
Agnieszka Padiasek ◽  
Barbara Żuromska-Witek ◽  
Marek Szlósarczyk
Keyword(s):  
Dietary Supplements ◽  
Vitamin K ◽  
Limit Of Detection ◽  
Volume Ratio ◽  
Pharmaceutical Products ◽  
Limit Of Quantification ◽  
Intermediate Precision ◽  
Clotting Cascade ◽  
Fat Soluble Vitamins ◽  
Analysis Of Vitamins

Vitamin K is a group of lipophilic molecules. Forms of vitamin K play an essential role in the activation of specific proteins involved in blood clotting cascade or bone metabolism. Another molecule belonging to the fat-soluble vitamins group that also plays an important role in calcium metabolism is vitamin D3. The dietary supplements containing vitamins K and D3 are one of the most frequently consumed by patients. The objective of this work was to develop a simple, fast and sensitive thin-layer chromatography (TLC)-densitometric procedure for the simultaneous quantitative analysis of vitamins K and D3 in pharmaceutical products and dietary supplements. The analysis of vitamins was performed on the silica gel RP-18 F₂₅₄s plates with methanol-ethanol-isopropanol in a volume ratio of 15:1:4 as a mobile phase. The densitometric measurements were made at 254 nm. The method was validated by checking the specificity, linearity, precision, recovery, limit of detection, limit of quantification and robustness in accordance with International Conference on Harmonization (ICH) guidelines. The method was shown to be specific, accurate (recoveries were from 95.78 to 104.96%), linear over the tested range (correlation coefficient, exceeding 0.99), and precise (precision and intermediate precision RSD below 2.70% for all analytes). The satisfactory results of the validation of the method indicate that it can be used in the quality control of dietary supplements and pharmaceutical products containing vitamins K and D3.

HPLC Analysis of Catechins, Theaflavins, and Alkaloids in Commercial Teas and Green Tea Dietary Supplements: Comparison of Water and 80% Ethanol/Water Extracts

2006 ◽  
Vol 71 (6) ◽  
pp. C328-C337 ◽  
Author(s):  
Mendel Friedman ◽  
Carol E. Levin ◽  
Suk-Hyun Choi ◽  
Etsuko Kozukue ◽  
Nobuyuki Kozukue
Keyword(s):  
Dietary Supplements ◽  
Green Tea ◽  
Hplc Analysis ◽  
Water Extracts

scholarly journals Influence of Renal Impairment and Genetic Subtypes on Warfarin Control in Japanese Patients

Genes ◽  
2021 ◽  
Vol 12 (10) ◽  
pp. 1537
Author(s):  
Tomotaka Tanaka ◽  
Masafumi Ihara ◽  
Kazuki Fukuma ◽  
Haruko Yamamoto ◽  
Kazuo Washida ◽  
...  
Keyword(s):  
Renal Impairment ◽  
Vitamin K ◽  
Optimal Algorithm ◽  
Linear Regression Analysis ◽  
Patient Characteristics ◽  
Japanese Patients ◽  
Multiple Linear Regression Analysis ◽  
Dietary Vitamin ◽  
Factors Affecting ◽  
Stroke Center

The genotypes of vitamin K epoxide reductase complex 1 (VKORC1) and cytochrome P450 2C9 (CYP2C9) can influence therapeutic warfarin doses. Conversely, nongenetic factors, especially renal function, are associated with warfarin maintenance doses; however, the optimal algorithm for considering genes and renal dysfunction has not been established. This single-center prospective cohort study aimed to evaluate the factors affecting warfarin maintenance doses and develop pharmacogenetics-guided algorithms, including the factors of renal impairment and others. To commence, 176 outpatients who were prescribed warfarin for thromboembolic stroke prophylaxis in the stroke center, were enrolled. Patient characteristics, blood test results, dietary vitamin K intake, and CYP2C9 and VKORC1 (−1639G > A) genotypes were recorded. CYP2C9 and VKORC1 (−1639G>A) genotyping revealed that 80% of the patients had CYP2C9*1/*1 and VKORC1 mutant AA genotypes. Multiple linear regression analysis demonstrated that the optimal pharmacogenetics-based model comprised age, body surface area, estimated glomerular filtration rate (eGFR), genotypes, vitamin K intake, aspartate aminotransferase levels, and alcohol intake. eGFR exercised a significant impact on the maintenance doses, as an increase in eGFR of 10 mL/min/1.73 m2 escalated the warfarin maintenance dose by 0.6 mg. Reduced eGFR was related to lower warfarin maintenance doses, independent of VKORC1 and CYP2C9 genotypes in Japanese patients.

Rapid and simple HPLC analysis of vitamin K in food, tissues and blood

2000 ◽  
Vol 68 (2) ◽  
pp. 219-221 ◽  
Author(s):  
E Jakob ◽  
I Elmadfa
Keyword(s):  
Vitamin K ◽  
Hplc Analysis

scholarly journals Lipidomic Profile and Enzymes Activity in Hepatic Microsomes of Rats in Physiological and Pathological Conditions

2021 ◽  
Vol 23 (1) ◽  
pp. 442
Author(s):  
Tomasz Lepionka ◽  
Małgorzata Białek ◽  
Marian Czauderna ◽  
Michał Szlis ◽  
Agnieszka Białek
Keyword(s):  
Fatty Acids ◽  
Dietary Supplements ◽  
Raw Materials ◽  
Folk Medicine ◽  
Antagonistic Effect ◽  
Pathological State ◽  
Sprague Dawley ◽  
Factors Affecting ◽  
Hepatic Microsomes ◽  
Cox 2

Among the risk factors affecting the development of cancer, nutritional factors occupy a significant place. Pomegranate seed oil (PSO) and bitter melon extract (BME), used for ages in folk medicine, are nowadays used in the prevention of many diseases and as ingredients of dietary supplements. Despite numerous publications on these raw materials or their active substances, their mechanism of action in various pathological states has not been recognized yet, nor has the safety of their simultaneous use been evaluated. The study aimed to assess how dietary supplementation with either PSO, with BME, or both, affects fatty acids’ profiles and their metabolism in hepatic microsomes, as well as the activity of selected microsomal enzymes (COX-2 and CYP1B1). Experimental animals (Sprague-Dawley rats) were divided into eight parallel experimental groups, differing in applied dietary modifications (control, PSO, BME and both PSO and BME) and introduction of chemical carcinogen—7,12-dimethylbenz[a]nthracene. Obtained results indicated the pronounced effect of the cancerous process on lipid metabolism and demonstrated the antagonistic effect of applied dietary supplements on the content of individual fatty acids and the activity of CYP1B1 and COX-2. The applied broad analytical approach and chemometric data analysis confirmed that raw materials, for which potential cancer prevention has been previously demonstrated, may differ in effects depending on the coexisting pathological state.

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