THE COMPARATIVE MARKETING ANALYSIS OF VITAMINS FROM ASSORTMENT GROUPS OF MEDICINAL DRUGS AND BIOLOGICALLY ACTIVE ADDITIVES

The article presents the results of a marketing study of an assortment of medicinal products and biologically active additives of vitamin action prescribed in a clinical children's hospital. Vitamin preparations are prescribed not only for the prevention of various diseases, but also in complex. Materials and research methods: The following methods were used in the work: content analysis, SWOT analysis, STEP analysis, statistical, graphic, sociological. The main information materials were: the Vidal reference book, the State Register of Medicines, price lists of pharmaceutical companies. Results: This article is about a marketing analysis of drugs and biologically active additives of vitamin action was carried out, consisting of a comparative analysis of the assortment, SWOT analysis, which revealed the competitiveness of dietary supplements in relation to drugs with such "strengths" as a large range, composition of natural origin and " opportunities ", in the form of the absence of restrictions in promotion. The negative characteristics of “weaknesses” and “threats” of dietary supplements scored more indicators. For STEP-analysis, we ranked the factors that influence the external environment. It was found that the economic component has the greatest impact on the implementation of drugs and dietary supplements of vitamin action, and the social one is the least significant. At the final stage, a comparative point assessment was carried out, which made it possible to single out vitamins - “leaders” from the assortment under study. Сonclusion: The obtained results are important for understanding consumer behavior and the formation of information material for the doctors.

Determination of Vitamins K1, K2 MK-4, MK-7, MK-9 and D3 in Pharmaceutical Products and Dietary Supplements by TLC-Densitometry

Vitamin K is a group of lipophilic molecules. Forms of vitamin K play an essential role in the activation of specific proteins involved in blood clotting cascade or bone metabolism. Another molecule belonging to the fat-soluble vitamins group that also plays an important role in calcium metabolism is vitamin D3. The dietary supplements containing vitamins K and D3 are one of the most frequently consumed by patients. The objective of this work was to develop a simple, fast and sensitive thin-layer chromatography (TLC)-densitometric procedure for the simultaneous quantitative analysis of vitamins K and D3 in pharmaceutical products and dietary supplements. The analysis of vitamins was performed on the silica gel RP-18 F₂₅₄s plates with methanol-ethanol-isopropanol in a volume ratio of 15:1:4 as a mobile phase. The densitometric measurements were made at 254 nm. The method was validated by checking the specificity, linearity, precision, recovery, limit of detection, limit of quantification and robustness in accordance with International Conference on Harmonization (ICH) guidelines. The method was shown to be specific, accurate (recoveries were from 95.78 to 104.96%), linear over the tested range (correlation coefficient, exceeding 0.99), and precise (precision and intermediate precision RSD below 2.70% for all analytes). The satisfactory results of the validation of the method indicate that it can be used in the quality control of dietary supplements and pharmaceutical products containing vitamins K and D3.

Simultaneous Determination of Vitamins D2 and D3 by Electrospray Ionization LC/MS/MS in Infant Formula and Adult Nutritionals: First Action 2012.11

A method was developed for the analysis of vitamins D2 and D3 in a variety of nutritional products. To extract vitamins D2 and D3 from products containing substantial amounts of fat, a saponification with alcoholic potassium hydroxide is required to release the vitamin D. Trideuterium- labeled vitamin D is added to the sample prior to saponification, and quantitation is achieved using linear regression of the ratio of peak response for 2H3-D and vitamin D. Acceptable linearity was achieved between 0.6 and 27 μg/100 g with a correlation requirement of >0.999. The method detection limit of 0.02 μg/100 g was verified by spiking placebo products carried through the saponification and extraction steps of the method. At the quantitation limit (0.12 μg/100 g), the signal was easily distinguished from the background. Vitamin D3 spike recoveries ranged from 107 to 119% at the low level and 104 to 116% at the high-level spike. Vitamin D2 recoveries were 105 to 116% and 91 to 110% for the low- and high-level spikes, respectively. SRM 1849a has a certified concentration of 11.1 ± 1.7 μg/100 g; using this standard reference material, the range of 9.4 to 12.8 μg/100 g was met on each of the 6 days. Method repeatability, determined in 12 vitamin D3 product matrixes over 6 days, ranged from 3.9 to 48%. The adult nutrition-milk protein sample was the most notable; it failed within-day, as well as day-to-day, precision requirements. There was no attempt to optimize the sample preparation to accommodate any problem matrix.