Processed purple‐fleshed potato prevents and protects against high‐fat diet elevated oxidative stress and inflammation markers in vivo in a pig model

Objective: The aim of the present study was to investigate the in vivo antioxidant potential of 50% ethanolic extract of Sesamum indicum against high-fat diet-induced rats. Methods: Animals were treated with plant extract for 30 d, and a high-fat diet was given to all groups except plain control, throughout, out the study. And alpha-tocopherol acetate (Vit, E) was used as standard. Pre-treatment with 16 mg/100 gm of body weight of 50% ethanolic extract of Sesamum indicum improved the Superoxide dismutase, catalase, glutathione, and lipid peroxidation levels significantly as compared to control group. Results: The present studies revealed that Sesamum indicum has significant in vivo antioxidant activity and can be used to protect tissue from oxidative stress. The result showed that the activities of SOD, catalase, lipid peroxidase, and glutathione, in the group treated with high-fat diet declined significantly than that of normal group. Conclusion: 50% ethanolic extract of in the dose of Sesamum indicum 16 mg/100 gm of body weight, has improved the SOD, catalase, glutathione, and lipid peroxidase levels significantly, which were comparable with high-fat-diet-induced rats. Based on this study we conclude that the 50% ethanolic extract of Sesamum indicum possesses in vivo antioxidant activity and can be employed in protecting tissue from oxidative stress.

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A novel method to detect in vivo oxidant production reveals increased susceptibility to high‐fat diet induced penile oxidative stress in GSNOR knockout mice (LB688)

The FASEB Journal ◽

10.1096/fasebj.28.1_supplement.lb688 ◽

2014 ◽

Vol 28 (S1) ◽

Author(s):

Justin La Favor ◽

Arthur Burnett

Keyword(s):

Oxidative Stress ◽

Knockout Mice ◽

High Fat Diet ◽

High Fat ◽

Novel Method ◽

Oxidant Production ◽

Increased Susceptibility

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LC-MS/MS Analysis and Hepatoprotective Activity of Artichoke (Cynara scolymusL.) Leaves Extract against High Fat Diet-Induced Obesity in Rats

BioMed Research International ◽

10.1155/2019/4851279 ◽

2019 ◽

Vol 2019 ◽

pp. 1-12 ◽

Cited By ~ 3

Author(s):

Maryem Ben Salem ◽

Kamilia Ksouda ◽

Raouia Dhouibi ◽

Slim Charfi ◽

Mouna Turki ◽

...

Keyword(s):

Oxidative Stress ◽

Lipid Profile ◽

High Fat Diet ◽

Ethanol Extract ◽

Hepatoprotective Activity ◽

Hepatic Tissue ◽

High Fat ◽

Cynara Scolymus ◽

Antioxidative Effects

Cynara scolymusL. (Artichoke) has been used for the treatment of metabolic disorders. The purpose of the present study was to investigate the hepatoprotective effect ofCynara scolymusleaves extract against a high fat diet (HFD) induced rats. This study investigated the most abundant phenolic compounds richCynara scolymusleaves extract and it is antihypercholesterolemic and antioxidative effectsin vivo. The hypercaloric high fat diet (HFD) was treated with 200 mg/kg and 400 mg/kg of ethanol extract (EEA) from leaves ofCynaraand atorvastatin (ATOR) (10 mg/kg/day) during an 8-week period. Lipid profile was measured and oxidative stress systematic in hepatic tissue was determined. Our data revealed that HFD-induced hepatic dysfunction manifested by significant abnormal levels of AST, ALT, ALP, LDH, and OCT was accompanied by increasing levels of oxidative stress biomarker (ROS, MDA, and AOPP) while decreasing in antioxidant status. Coadministration of EEA significantly reduced serum lipid profile and hepatic disorders which was confirmed to be histological by reducing the fatty liver deposition in hepatic lobule. These findings suggest thatCynaraleaves exert antiobesity and antioxidant liver effects in HFD-induced obese rats.

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A Novel Multi-Ingredient Supplement Activates a Browning Program in White Adipose Tissue and Mitigates Weight Gain in High-Fat Diet-Fed Mice

Nutrients ◽

10.3390/nu13113726 ◽

2021 ◽

Vol 13 (11) ◽

pp. 3726

Author(s):

Joshua P. Nederveen ◽

Katherine Manta ◽

Adam L. Bujak ◽

Alexander C. Simone ◽

Matthew R. Fuda ◽

...

Keyword(s):

Adipose Tissue ◽

Fat Mass ◽

White Adipose Tissue ◽

High Fat Diet ◽

Fatty Acid Transport ◽

Acid Transport ◽

Inflammation Markers ◽

High Fat ◽

Brown Adipose

We investigated the effects of a novel multi-ingredient supplement comprised of polyphenol antioxidants and compounds known to facilitate mitochondrial function and metabolic enhancement (ME) in a mouse model of obesity. In this study, 6-week-old male C57/BL6J mice were placed on a high-fat diet (HFD; ~60% fat) for 6 weeks, with subsequent allocation into experimentalgroups for 4 weeks: HFD control, HFD + ME10 (10 components), HFD + ME7 (7 components), HFD + ME10 + EX, HFD + EX (where ‘+EX’ animals exercised 3 days/week), and chow-fed control. After the intervention, HFD control animals had significantly greater body weight and fat mass. Despite the continuation of HFD, animals supplemented with multi-ingredient ME or who performed exercise training showed an attenuation of fat mass and preservation of lean body mass, which was further enhanced when combined (ME+EX). ME supplementation stimulated the upregulation of white and brown adipose tissue mRNA transcripts associated with mitochondrial biogenesis, browning, fatty acid transport, and fat metabolism. In WAT depots, this was mirrored by mitochodrial oxidative phosphorylation (OXPHOS) protein expression, and increased in vivo fat oxidation measured via CLAMS. ME supplementation also decreased systemic and local inflammation markers. Herein, we demonstrated that novel multi-ingredient nutritional supplements induced significant fat loss independent of physical activity while preserving muscle mass in obese mice. Mechanistically, these MEs appear to act by inducing a browning program in white adipose tissue and decreasing other pathophysiological impairments associated with obesity, including mitochondrial respiration alterations induced by HFD.

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Indicaxanthin from Opuntia ficus-indica Fruit Ameliorates Glucose Dysmetabolism and Counteracts Insulin Resistance in High-Fat-Diet-Fed Mice

Antioxidants ◽

10.3390/antiox11010080 ◽

2021 ◽

Vol 11 (1) ◽

pp. 80

Author(s):

Simona Terzo ◽

Alessandro Attanzio ◽

Pasquale Calvi ◽

Flavia Mulè ◽

Luisa Tesoriere ◽

...

Keyword(s):

Oxidative Stress ◽

Insulin Resistance ◽

Adipose Tissue ◽

High Fat Diet ◽

High Fat ◽

Opuntia Ficus Indica ◽

Beneficial Effects ◽

Reduced Body

Obesity-related dysmetabolic conditions are amongst the most common causes of death globally. Indicaxanthin, a bioavailable betalain pigment from Opuntia ficus-indica fruit, has been demonstrated to modulate redox-dependent signalling pathways, exerting significant anti-oxidative and anti-inflammatory effects in vitro and in vivo. In light of the strict interconnections between inflammation, oxidative stress and insulin resistance (IR), a nutritionally relevant dose of indicaxanthin has been evaluated in a high-fat diet (HFD) model of obesity-related IR. To this end, biochemical and histological analysis, oxidative stress and inflammation evaluations in liver and adipose tissue were carried out. Our results showed that indicaxanthin treatment significantly reduced body weight, daily food intake and visceral fat mass. Moreover, indicaxanthin administration induced remarkable, beneficial effects on HFD-induced glucose dysmetabolism, reducing fasting glycaemia and insulinaemia, improving glucose and insulin tolerance and restoring the HOMA index to physiological values. These effects were associated with a reduction in hepatic and adipose tissue oxidative stress and inflammation. A decrease in RONS, malondialdehyde and NO levels, in TNF-α, CCL-2 and F4-80 gene expression, in p65, p-JNK, COX-2 and i-NOS protein levels, in crown-like structures and hepatic inflammatory foci was, indeed, observed. The current findings encourage further clinical studies to confirm the effectiveness of indicaxanthin to prevent and treat obesity-related dysmetabolic conditions.

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In vivo anti-oxidant activity of Dhatrinisha churna and Manjisthadi churna in high fat diet induced oxidative stress

Free Radicals and Antioxidants ◽

10.5530/ax.2012.2.2.4 ◽

2012 ◽

Vol 2 (2) ◽

pp. 19-23 ◽

Cited By ~ 1

Author(s):

R. Patel Vishal ◽

K. Patel Rakesh

Keyword(s):

Oxidative Stress ◽

High Fat Diet ◽

High Fat ◽

Anti Oxidant

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Effects of resveratrol on oxidative stress in high fat diet /streptozocin induced diabetic wistar albino rats

International Journal of Basic & Clinical Pharmacology ◽

10.18203/2319-2003.ijbcp20190651 ◽

2019 ◽

Vol 8 (3) ◽

pp. 482 ◽

Cited By ~ 2

Author(s):

Smita Das ◽

Jayanti Prava Behera ◽

Y. Rojaramani ◽

Rashmi Ranjan Mohanty

Keyword(s):

Oxidative Stress ◽

Blood Glucose ◽

High Fat Diet ◽

Fasting Blood Glucose ◽

Diabetic Control ◽

Albino Rats ◽

High Fat ◽

Common Chronic Disease ◽

Wistar Albino Rats

Background: Diabetes mellitus is a common chronic disease. One of the pathophysiology is found to be oxidative stress. This study aims to evaluate the effect of resveratrol on oxidative stress in high fat diet (HFD)/streptozotocin induced diabetic rats.Methods: Wistar albino rats, fed with HFD rendered diabetic with streptozotocin, were divided into 6 groups, namely the diabetic control treated with vehicle (DC), standard control which received metformin (SC), test groups treated with 5,10, and 20 mg/kg b.w. of resveratrol and combination of half dose of metformin and resveratrol (10 mg/kg)(TC). A group of six normal animals served as normal control (NC), another six as HFD control. Fasting blood glucose, lipid profile and serum MDA and SOD were measured one week after induction of diabetes. The animals were then treated orally for 2 weeks after which the same parameters were repeated. The in-vivo results were analysed by one way ANOVA followed by Tukey’s multiple comparison test.Results: The DC group demonstrated a increase in the fasting blood glucose compared to NC, HFD control while a significant decrease in the fasting blood glucose was observed with SC, Test groups (p<0.05) as compared to the DC group. TC showed a significant improvement in dyslipidemia compared to their baseline values (p<0.05). There was significant change in the serum MDA level and SOD activity.Conclusions: Resveratrol improves oxidative stress in diabetic rats.

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EVALUATION OF IN VIVO ANTIOXIDANT ACTIVITY OF HYDRO ALCOHOLIC EXTRACT OF LINUM USITATISSIMUM L. (TUKHM-E-KATAN) AGAINST HIGH FAT DIET INDUCED RATS

INDIAN DRUGS ◽

10.53879/id.56.02.11522 ◽

2019 ◽

Vol 56 (02) ◽

pp. 64-72

Author(s):

Z. J Khan ◽

◽

N. A. Khan ◽

I Naseem ◽

S. A. A. Nami

Keyword(s):

Oxidative Stress ◽

Antioxidant Activity ◽

Superoxide Dismutase ◽

Linum Usitatissimum ◽

High Fat Diet ◽

Ethanolic Extract ◽

Control Group ◽

Alcoholic Extract ◽

High Fat

The aim of the present study was to evaluate the in vivo antioxidant activity of 50% ethanolic extract of Linum usitatissimum against high fat diet induced rats. Animals were treated with plant extract for 30 days, and high fat diet was given to all groups except plain control through, out the study, and alpha tocopherol acetate (Vit, E) was used as standard. pre-treatment with 23 mg/100 gm of body weight of 50% ethanolic extract of Linum usitatissimum significantly improved the superoxide dismutase, catalase, glutathione, and lipid peroxidation levels as compared to control group. The present studies revealed that the in vivo antioxidant activity of Linum usitatissimum was significant, and can be used to protect tissue from oxidative stress. The result showed that the superoxide dismutase, catalase, lipid peroxidase, and glutathione reductase activities significantly declined in group treated with high fat diet than that of normal group. Based on this investigation, it was concluded that the 50% ethanolic extract of Linum usitatissimum has good in vivo antioxidant activity and can be used in protecting tissue from oxidative stress.

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