75: MICRORNA-1 AND MICRORNA-206 IMPROVE HUMAN SATELLITE CELL DIFFERENTIATION IN VITRO: A NOVEL APPROACH FOR TISSUE ENGINEERING OF SKELETAL MUSCLE

2011 ◽  
Vol 127 ◽  
pp. 46
Author(s):  
M Koning ◽  
MC Harmsen ◽  
RA Bank ◽  
PM Werker
Keyword(s):  
Skeletal Muscle ◽  
Tissue Engineering ◽  
Cell Differentiation ◽  
Satellite Cell ◽  
Novel Approach

scholarly journals Recycled algae-based carbon materials as electroconductive 3D printed skeletal muscle tissue engineering scaffolds

2021 ◽  
Vol 32 (7) ◽  
Author(s):  
Selva Bilge ◽  
Emre Ergene ◽  
Ebru Talak ◽  
Seyda Gokyer ◽  
Yusuf Osman Donar ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Tissue Engineering ◽  
Electrical Stimulation ◽  
Muscle Tissue ◽  
Carbonaceous Material ◽  
Hydrothermal Carbonization ◽  
Skeletal Muscle Tissue ◽  
Myotube Formation ◽  
3D Printed

AbstractSkeletal muscle is an electrically and mechanically active tissue that contains highly oriented, densely packed myofibrils. The tissue has self-regeneration capacity upon injury, which is limited in the cases of volumetric muscle loss. Several regenerative therapies have been developed in order to enhance this capacity, as well as to structurally and mechanically support the defect site during regeneration. Among them, biomimetic approaches that recapitulate the native microenvironment of the tissue in terms of parallel-aligned structure and biophysical signals were shown to be effective. In this study, we have developed 3D printed aligned and electrically active scaffolds in which the electrical conductivity was provided by carbonaceous material (CM) derived from algae-based biomass. The synthesis of this conductive and functional CM consisted of eco-friendly synthesis procedure such as pre-carbonization and multi-walled carbon nanotube (MWCNT) catalysis. CM obtained from biomass via hydrothermal carbonization (CM-03) and its ash form (CM-03K) were doped within poly(ɛ-caprolactone) (PCL) matrix and 3D printed to form scaffolds with aligned fibers for structural biomimicry. Scaffolds were seeded with C2C12 mouse myoblasts and subjected to electrical stimulation during the in vitro culture. Enhanced myotube formation was observed in electroactive groups compared to their non-conductive counterparts and it was observed that myotube formation and myotube maturity were significantly increased for CM-03 group after electrical stimulation. The results have therefore showed that the CM obtained from macroalgae biomass is a promising novel source for the production of the electrically conductive scaffolds for skeletal muscle tissue engineering.

scholarly journals Bioengineered in vitro skeletal muscles as new tools for muscular dystrophies preclinical studies

2021 ◽  
Vol 12 ◽  
pp. 204173142098133
Author(s):  
Juan M. Fernández-Costa ◽  
Xiomara Fernández-Garibay ◽  
Ferran Velasco-Mallorquí ◽  
Javier Ramón-Azcón
Keyword(s):  
Skeletal Muscle ◽  
Tissue Engineering ◽  
Electrical Stimulation ◽  
Skeletal Muscles ◽  
Screening Tools ◽  
Muscular Dystrophies ◽  
Preclinical Studies ◽  
Technological Advances ◽  
Topographical Cues

Muscular dystrophies are a group of highly disabling disorders that share degenerative muscle weakness and wasting as common symptoms. To date, there is not an effective cure for these diseases. In the last years, bioengineered tissues have emerged as powerful tools for preclinical studies. In this review, we summarize the recent technological advances in skeletal muscle tissue engineering. We identify several ground-breaking techniques to fabricate in vitro bioartificial muscles. Accumulating evidence shows that scaffold-based tissue engineering provides topographical cues that enhance the viability and maturation of skeletal muscle. Functional bioartificial muscles have been developed using human myoblasts. These tissues accurately responded to electrical and biological stimulation. Moreover, advanced drug screening tools can be fabricated integrating these tissues in electrical stimulation platforms. However, more work introducing patient-derived cells and integrating these tissues in microdevices is needed to promote the clinical translation of bioengineered skeletal muscle as preclinical tools for muscular dystrophies.

scholarly journals PEDF-derived peptide promotes skeletal muscle regeneration through its mitogenic effect on muscle progenitor cells

2015 ◽  
Vol 309 (3) ◽  
pp. C159-C168 ◽  
Author(s):  
Tsung-Chuan Ho ◽  
Yi-Pin Chiang ◽  
Chih-Kuang Chuang ◽  
Show-Li Chen ◽  
Jui-Wen Hsieh ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Cell Proliferation ◽  
Cyclin D1 ◽  
Satellite Cell ◽  
Muscle Regeneration ◽  
Muscle Fibers ◽  
Skeletal Muscle Regeneration ◽  
Satellite Cell Proliferation

In response injury, intrinsic repair mechanisms are activated in skeletal muscle to replace the damaged muscle fibers with new muscle fibers. The regeneration process starts with the proliferation of satellite cells to give rise to myoblasts, which subsequently differentiate terminally into myofibers. Here, we investigated the promotion effect of pigment epithelial-derived factor (PEDF) on muscle regeneration. We report that PEDF and a synthetic PEDF-derived short peptide (PSP; residues Ser93-Leu112) induce satellite cell proliferation in vitro and promote muscle regeneration in vivo. Extensively, soleus muscle necrosis was induced in rats by bupivacaine, and an injectable alginate gel was used to release the PSP in the injured muscle. PSP delivery was found to stimulate satellite cell proliferation in damaged muscle and enhance the growth of regenerating myofibers, with complete regeneration of normal muscle mass by 2 wk. In cell culture, PEDF/PSP stimulated C2C12 myoblast proliferation, together with a rise in cyclin D1 expression. PEDF induced the phosphorylation of ERK1/2, Akt, and STAT3 in C2C12 myoblasts. Blocking the activity of ERK, Akt, or STAT3 with pharmacological inhibitors attenuated the effects of PEDF/PSP on the induction of C2C12 cell proliferation and cyclin D1 expression. Moreover, 5-bromo-2′-deoxyuridine pulse-labeling demonstrated that PEDF/PSP stimulated primary rat satellite cell proliferation in myofibers in vitro. In summary, we report for the first time that PSP is capable of promoting the regeneration of skeletal muscle. The signaling mechanism involves the ERK, AKT, and STAT3 pathways. These results show the potential utility of this PEDF peptide for muscle regeneration.

scholarly journals Effects of intramuscular administration of 1α,25(OH)2D3 during skeletal muscle regeneration on regenerative capacity, muscular fibrosis, and angiogenesis

2016 ◽  
Vol 120 (12) ◽  
pp. 1381-1393 ◽  
Author(s):  
Ratchakrit Srikuea ◽  
Muthita Hirunsai
Keyword(s):  
Skeletal Muscle ◽  
Vitamin D ◽  
Protein Synthesis ◽  
Cell Differentiation ◽  
Satellite Cell ◽  
Muscle Regeneration ◽  
Fiber Type ◽  
Fiber Formation ◽  
Skeletal Muscle Regeneration ◽  
Type Composition

The recent discovery of the vitamin D receptor (VDR) in regenerating muscle raises the question regarding the action of vitamin D3 on skeletal muscle regeneration. To investigate the action of vitamin D3 on this process, the tibialis anterior muscle of male C57BL/6 mice (10 wk of age) was injected with 1.2% BaCl2 to induce extensive muscle injury. The bioactive form of vitamin D3 [1α,25(OH)2D3] was administered daily via intramuscular injections during the regenerative phase (days 4-7 postinjury). Physiological and supraphysiological doses of 1α,25(OH)2D3 relative to 1 μg/kg muscle wet weight and mouse body weight were investigated. Muscle samples were collected on day 8 postinjury to examine proteins related to vitamin D3 metabolism (VDR, CYP24A1, and CYP27B1), satellite cell differentiation and regenerative muscle fiber formation [myogenin and embryonic myosin heavy chain (EbMHC)], protein synthesis signaling (Akt, p70 S6K1, 4E-BP1, and myostatin), fiber-type composition (fast and slow MHCs), fibrous formation (vimentin), and angiogenesis (CD31). Administration of 1α,25(OH)2D3 at physiological and supraphysiological doses enhanced VDR expression in regenerative muscle. Moreover, CYP24A1 and vimentin expression was increased, accompanying decreased myogenin and EbMHC expression at the supraphysiological dose. However, there was no change in CYP27B1, Akt, p70 S6K1, 4E-BP1, myostatin, fast and slow MHCs, or CD31 expression at any dose investigated. Taken together, administration of 1α,25(OH)2D3 at a supraphysiological dose decreased satellite cell differentiation, delayed regenerative muscle fiber formation, and increased muscular fibrosis. However, protein synthesis signaling, fiber-type composition, and angiogenesis were not affected by either 1α,25(OH)2D3 administration at a physiological or supraphysiological dose.

Effect ofECM2expression on bovine skeletal muscle-derived satellite cell differentiation

2018 ◽  
Vol 42 (5) ◽  
pp. 525-532 ◽  
Author(s):  
Chang Liu ◽  
Huili Tong ◽  
Shufeng Li ◽  
Yunqin Yan
Keyword(s):  
Skeletal Muscle ◽  
Cell Differentiation ◽  
Satellite Cell ◽  
Bovine Skeletal Muscle

scholarly journals A simplified method for tissue engineering skeletal muscle organoids in vitro

1997 ◽  
Vol 33 (9) ◽  
pp. 659-661 ◽  
Author(s):  
Janet Shansky ◽  
Joseph Chromiak ◽  
Michael Del Tatto ◽  
Herman Vandenburgh
Keyword(s):  
Skeletal Muscle ◽  
Tissue Engineering ◽  
Simplified Method

scholarly journals Atypical Protein Kinase Cs Are the Ras Effectors That Mediate Repression of Myogenic Satellite Cell Differentiation

2002 ◽  
Vol 22 (4) ◽  
pp. 1140-1149 ◽  
Author(s):  
Yuri V. Fedorov ◽  
Nathan C. Jones ◽  
Bradley B. Olwin
Keyword(s):  
Skeletal Muscle ◽  
Cell Proliferation ◽  
Cell Differentiation ◽  
Cell Line ◽  
Satellite Cell ◽  
Myogenic Differentiation ◽  
Ectopic Expression ◽  
Dominant Negative ◽  
Dependent Inhibition ◽  
Ras Effectors

ABSTRACT Oncogenic Ha-Ras is a potent inhibitor of skeletal muscle cell differentiation, yet the Ras effector mediating this process remains unidentified. Here we demonstrate that the atypical protein kinases (aPKCs; λ and/or ζ) are downstream Ras effectors responsible for Ras-dependent inhibition of myogenic differentiation in a satellite cell line. First, ectopic expression of Ha-RasG12V induces translocation of PKCλ from the cytosol to the nucleus, suggesting that aPKCs are activated by Ras in myoblasts. The aPKCs function as downstream Ras effectors since inhibition of aPKCs by expression of a dominant negative PKCζ mutant or by treatment of cells with an inhibitor, GO6983, promotes myogenesis in skeletal muscle satellite cells expressing oncogenic Ha-Ras. Arresting cell proliferation synergistically enhances myogenic differentiation only when aPKCs are also inhibited. Thus, the repression of myogenic differentiation in a satellite cell line appears to be directly mediated by aPKCs acting as Ras effectors and indirectly mediated via stimulation of cell proliferation.

In vivo expression patterns of MyoD, p21, and Rb proteins in myonuclei and satellite cells of denervated rat skeletal muscle

2004 ◽  
Vol 287 (2) ◽  
pp. C484-C493 ◽  
Author(s):  
Minenori Ishido ◽  
Katsuya Kami ◽  
Mitsuhiko Masuhara
Keyword(s):  
Skeletal Muscle ◽  
Satellite Cell ◽  
Time Course ◽  
Kinase Inhibitor ◽  
Expression Patterns ◽  
Cell Types ◽  
Cell Nuclei ◽  
Myogenic Regulatory Factor

MyoD, a myogenic regulatory factor, is rapidly expressed in adult skeletal muscles in response to denervation. However, the function(s) of MyoD expressed in denervated muscle has not been adequately elucidated. In vitro, it directly transactivates cyclin-dependent kinase inhibitor p21 (p21) and retinoblastoma protein (Rb), a downstream target of p21. These factors then act to regulate cell cycle withdrawal and antiapoptotic cell death. Using immunohistochemical approaches, we characterized cell types expressing MyoD, p21, and Rb and the relationship among these factors in the myonucleus of denervated muscles. In addition, we quantitatively examined the time course changes and expression patterns among distinct myofiber types of MyoD, p21, and Rb during denervation. Denervation induced MyoD expression in myonuclei and satellite cell nuclei, whereas p21 and Rb were found only in myonuclei. Furthermore, coexpression of MyoD, p21, and Rb was induced in the myonucleus, and quantitative analysis of these factors determined that there was no difference among the three myofiber types. These observations suggest that MyoD may function in myonuclei in response to denervation to protect against denervation-induced apoptosis via perhaps the activation of p21 and Rb, and function of MyoD expressed in satellite cell nuclei may be negatively regulated. The present study provides a molecular basis to further understand the function of MyoD expressed in the myonuclei and satellite cell nuclei of denervated skeletal muscle.

Tissue Engineered Bone Formation with Polymer/Ceramic Composites by Press-and-Baking Method

2005 ◽  
Vol 288-289 ◽  
pp. 79-82 ◽  
Author(s):  
Young Mee Jung ◽  
Soo Hyun Kim ◽  
Sang Soo Kim ◽  
Hee Jin You ◽  
Byoung Soo Kim ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Differentiation ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Direct Interaction ◽  
Ceramic Composites ◽  
Calcium Deposition ◽  
Solvent Casting ◽  
Differentiation And Proliferation

A novel process was developed to fabricate polymer/ceramic composites for bone tissue engineering. The mixture of polylactic acid (PLA), calcium metaphosphate (CMP), and NaCl were compressed and subsequently heated. After dissolving the NaCl salts, porous biodegradable polymer/ceramic composite scaffolds were formed. The characteristics of the scaffolds were compared to those of scaffolds fabricated using a conventional solvent casting method, in terms of pore structure, pore size distribution, and mechanical properties. The scaffolds were seeded by osteoblasts and cultured in vitro or implanted into nude mice subcutaneously for up to 5 weeks. Cells were better grown to form tissue-like structures on CMP/PLA composites fabricated by the Press-and-Baking method. In addition, the alkaline phosphatase activity of and calcium deposition in the scaffolds explanted from mice were enhanced significantly for the scaffolds by Press-and-Baking compared to them by solvent casting. Taken together, these results suggest that CMP promote cell differentiation and proliferation via direct interaction with cells in the CMP/PLA composites. This novel PLA/CMP composite will be applicable for bone tissue engineering to support and cell differentiation and growth.

scholarly journals Applications of Small Molecules in Muscle Tissue Engineering

2021 ◽  
Author(s):  
Behnaz Mirza Ahmadi ◽  
Mahmood Talkhabi ◽  
Sarah Rajabi
Keyword(s):  
Skeletal Muscle ◽  
Tissue Engineering ◽  
Small Molecules ◽  
Muscle Tissue ◽  
Muscle Regeneration ◽  
Cost Effective ◽  
Clinical Approach ◽  
Muscle Injuries ◽  
Inducing Factors

Introduction: Skeletal muscles account for about 40% of the total body weight. Every year, hundreds of people lose at least part of their muscle tissue due to illness, war, and accidents. This can lead to disruption of activities such as breathing, movement, and social life. To this end, various therapeutic strategies such as medication therapy, cell therapy and tissue transplantation have been used or studied in muscle regeneration. However, there is no effective and well-defined clinical approach for treatment of muscle injuries and the severity of muscle injuries increase with age in most cases. Therefore, investigation for finding new and effective clinical approach for muscle regeneration is one of the most important issues in basic and clinical researches. Tissue engineering is considered as one of the promising and newest approach for skeletal muscle tissue regeneration and provides an appropriate model for personalized medicine and basic researches that can be used in personalized medicine and basic research. Besides biomaterials and cells, inducing factors are another element of tissue engineering. These factors influence epigenetic mechanisms and signaling pathway, thereby inducing proliferation, differentiation, and migration of cells used in muscle tissue engineering, and accelerates muscle formation in vitro. Recently, small molecules have been used as alternatives to growth factors or along with other inducing factors in muscle tissue engineering. Since they do not induce an immune reaction, penetrate easily to the cells and have a specific molecular target, therefore they have attracted much attention as the cost-effective inducing factors in tissue engineering. Conclusion:  Taken together, the effective small molecules in muscle tissue engineering can be used with different biomaterial conditions (e.g. hydrogel, decellularized tissue, and synthetic scaffolds) in both in vivo and in vitro, resulting to production of cost effective and highly efficient engineered muscle tissues that help to achieve therapeutical goals of muscle tissue engineering. Herein, we describe tissue engineering and review the small molecules used in skeletal muscle tissue engineering.

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