scholarly journals The wettability of electron spun membranes by synovial fluid

2021 ◽  
Vol 8 (12) ◽  
Author(s):  
Ales Hrouda ◽  
Radek Jirkovec ◽  
Petra Hamrikova ◽  
Maarten Vanierschot ◽  
Kathleen Denis ◽  
...  

Aseptic loosening due to periprosthetic osteolysis has been accepted as one of the leading causes of revision procedures in patients with previous joint arthroplasty. Recently, several strategies for suppression of osteolysis were proposed, mostly based on biological treatment such as mitigation of chronic inflammatory reactions. However, these biological treatments do not stop the debris migration but only reduce the inflammatory reaction. To address this shortcoming, we propose the concept of ultrahigh molecular weighted polyethylene particles filtration storage by electrospun membranes. Firstly, the surface tension of synovial fluid (SF) is obtained by use of a pendant droplet. Secondly, the contact angle of the electrospun membranes wetted by two different liquids is measured to obtain the free surface energy using of the Owens–Wendt model. Additionally, the wettability of electrospun membranes by SF as a function of technology parameters is studied.

Author(s):  
Hamed A. A. Omer ◽  
Sawsan M. Ahmed ◽  
Roshdy I. El-Kady ◽  
Aly A. El-Shahat ◽  
Mahmoud Y. El-Ayek ◽  
...  

Abstract Background Agriculture by-products are considered a great potential value for utilization by ruminants as well as rabbits. They usually can be the maintenance and part of the production requirements. However, in developing countries, as well as in Egypt, animals suffer from shortage of feeds that are continuously increasing in costs. In general, biological treatments were shown to be the most effective and improved chemical composition of rice straw or corn stalks. Method This work aimed to investigate the possible ways of utilizing rice straws or corn stalks in rabbit feeding. The field work is designed to study the effect of biological treatment of Pleurotus ostreatus cultivated on rice straws and Trichoderma reesei cultivated on corn stalks and replacing clover hay by rice straws and corn stalks at levels of 0, 33, 66, and 100% either without or with microbes adding. Seventy-eight New Zealand White (NZW) rabbits aged 4–5 weeks (565 ± 13.57 g) were randomly divided into thirteen equal experimental groups. Results Untreated rice straws or biologically treated with Pleurotus ostreatus increased their contents of crude protein (CP) by 178.75 and 224.5% and nitrogen-free extract (NFE) by 6.30 and 24.53, respectively. Meanwhile, crude fiber (CF) content was reduced by 31.32 and 56.75%, and organic matter content was decreased by 2.81 and 5.51%, respectively, in comparison with the raw rice straws. Also, biological treatment of rice straws caused a decrease in values of neutral detergent fiber (NDF), acid detergent fiber (ADF), acid detergent lignin (ADL), and hemicellulose contents in comparison with either raw or treated rice straws. Furthermore, biological treatment with Trichoderma reesei realized a decrease in organic matter (OM), CF, NDF, and ADF and increased CP and ash contents in corn stalks. NFE content of corn stalks was decreased as a result of treatment without or with Trichoderma reesei experimental rations by 11.95% and 3.82% compared to raw corn stalks (CS). Biological treatments with fungi significantly (P < 0.05) improved average daily gain (ADG) and feed conversion. ADG and feed conversion were significantly (P < 0.05) improved when rabbits were fed diets containing rice straw (RS) compared to that fed CS. Rabbits fed diets replaced clover hay (CH) with 33% or 66% of RS or CS significantly increased ADG compared to control and that replaced 100% of both RS and CS containing rations. Levels of replacing had no significant effect on their dry matter intake (DMI) values. The highest improvement in feed conversion was recorded with rabbits that received diets replaced 33% of berseem hay (BH) by RS or CS, followed by that replaced 66% of BH by RS or CS. There were significantly interactions between biological treatments (T), roughage source (S), and replacement levels (L) (T × S × L) only on ADG. The best fed conversion was realized by rabbits fed diet replaced BH with 33% of RS that are treated by Pleurotus ostreatus (4.05 g DMI/g gain). Rabbits fed 33% biologically treated rice straw with Pleurotus ostreatus showed the highest economic efficiency (179%) followed by rabbits that received 33% of both rice straws treated without Pleurotus ostreatus and rabbits that received corn stalks biologically treated with Trichoderma reesei (161%). Conclusion Biological treatments of rice straws by Pleurotus ostreatus or corn stalks by Trichoderma reesei were safe, and it improves their chemical analysis and improved both daily gain and feed conversion, decreasing the costing of diet formulation which consequently decreased the price of 1-kg live body weight.


2017 ◽  
Vol 14 (2) ◽  
pp. 148-152 ◽  
Author(s):  
Samir K. Trehan ◽  
Lester Zambrana ◽  
Jonathan E. Jo ◽  
Ed Purdue ◽  
Athanos Karamitros ◽  
...  

2020 ◽  
Vol 11 ◽  
Author(s):  
Yuan Tian ◽  
Mohamad Alaa Terkawi ◽  
Tomohiro Onodera ◽  
Hend Alhasan ◽  
Gen Matsumae ◽  
...  

Author(s):  
Pedro Hinarejos ◽  
Ignasi Piñol ◽  
Alberto Torres ◽  
Daniel Valverde ◽  
Gabriel Gil-Gómez ◽  
...  

Lubricants ◽  
2015 ◽  
Vol 3 (2) ◽  
pp. 394-412 ◽  
Author(s):  
Michael Kung ◽  
John Markantonis ◽  
Scott Nelson ◽  
Patricia Campbell

1983 ◽  
Vol 17 (9) ◽  
pp. 645-648 ◽  
Author(s):  
William J. Cady ◽  
Bradley G. Wulf ◽  
Michael T. O'Neil ◽  
Dwight W. Burney ◽  
William R. Hamsa

Total joint arthroplasty is a common orthopedic procedure and requires prophylactic antibiotic coverage to prevent infections in the operated joint. The antibiotics routinely used for prophylaxis are the cephalosporins. This study compared bone, synovial fluid, and plasma concentrations of ceforanide with cephalothin concentrations in 30 patients undergoing elective total hip or total knee arthroplasty. Ceforanide provided significantly higher plasma concentrations for 61–110 minutes postdose than did cephalothin (p < 0.025 and p < 0.005). No difference was noted between the two antibiotics for the bone concentrations in the total hip arthroplasty group; however, cephalothin concentrated to a greater degree in the bone of patients undergoing total knee arthroplasty (p < 0.05). Cephalothin achieved higher concentrations in the synovial fluid than did ceforanide (p < 0.05). Both antibiotics were well tolerated and no postoperative infections were noted in either group.


2020 ◽  
Vol 9 (2) ◽  
pp. 516 ◽  
Author(s):  
Julian M. Rüwald ◽  
Thomas M. Randau ◽  
Cäcilia Hilgers ◽  
Werner Masson ◽  
Stephan Irsen ◽  
...  

Extracellular vesicles (EVs) comprise an as yet insufficiently investigated intercellular communication pathway in the field of revision total joint arthroplasty (RTJA). This study examined whether periprosthetic joint synovial fluid contains EVs, developed a protocol for their isolation and characterized them with respect to quantity, size, surface markers as well as documented their differences between aseptic implant failure (AIF) and periprosthetic joint infection (PJI). EV isolation was accomplished using ultracentrifugation, electron microscopy (EM) and nanoparticle tracking analysis evaluated EV presence as well as particle size and quantity. EV surface markers were studied by a bead-based multiplex analysis. Using our protocol, EM confirmed the presence of EVs in periprosthetic joint synovial fluid. Higher EV particle concentrations and decreased particle sizes were apparent for PJI. Multiplex analysis confirmed EV-typical surface epitopes and revealed upregulated CD44 and HLA-DR/DP/DQ for AIF, as well as increased CD40 and CD105. Our protocol achieved isolation of EVs from periprosthetic joint synovial fluid, confirmed by EM and multiplex analysis. Characterization was documented with respect to size, concentration and epitope surface signature. Our results indicate various differences between PJI and AIF EVs. This pilot study enables new research approaches and rising diagnostic opportunities in the field of RTJA.


2019 ◽  
Vol 25 ◽  
pp. 107602961982811 ◽  
Author(s):  
Chase Thorson ◽  
Kevin Galicia ◽  
Andrew Burleson ◽  
Olivia Bouchard ◽  
Debra Hoppensteadt ◽  
...  

Osteoarthritis, a degenerative disease of the joints, is the most common form of arthritis in the knee. Total joint arthoplasty is a commonly used treatment for joint degeneration and osteoarthritis, and due to these factors, TJA for hip and knee joints is projected to grow by 137% and 601% between 2005 and 2030. Matrix metalloproteases are enzymes found in the extracellular matrix that cleave matrix components. Normally MMPs are downregulated in tissues by Tissue Inhibitors of Metalloproteases, or TIMPs. The relative concentration of TIMPs also may denote some of the activity of the MMPs found in serum. Lubricin (proteoglycan 4) is a molecule found in the synovial fluid that protects joints by dissipating strain energy during locomotion. Lubricin synovial fluid concentration is also diminished in many patients with osteoarthritis, but not all. Given the importance of these three sets of molecules, our lab investigated the correlation between circulating lubricin, MMP levels and TIMPs levels. Blood plasma samples were obtained from de-identified subjects undergoing total joint arthroplasty at Loyola University Medical Center and the University of Utah. Normal blood plasma from pooled healthy individuals served as a control. We analyzed biomarker levels in plasma using ELISA. Our data show that MMP-1 and 9 were increased in TJA patients compared to normal controls, while MMP-2 and 13 were decreased. We also found decreased lubricin and tissue factor in surgical patients relative to controls. These data support the idea that lubricin is vital in protecting the synovial joint and that MMPs play a complex role in the destruction of the joint.


Author(s):  
Nawel Nedjah ◽  
Oualid Hamdaoui ◽  
Nabila Laskri

Algeria has a fragile ecosystem, characterized by a relative scarcity of water resources and their preservation is a vital imperative. The excessive intake in the natural environment of nitrogen and phosphate load in wastewater, from agricultural activities, domestic and industrial pollution, represents a pollution threat and a serious danger to the aquatic environment. Municipal wastewater contains various wastes, and usually consists of about 99.94% liquid and 0.06% solids. If this wastewater is not treated or improperly collected, it can affect both public health and environment. In our study, we will focus on the biological treatment by natural lagoon and activated sludge, which suits perfectly our country, where space and climate are favorable factors; our main objective is to eliminate the maximum existing phosphorus in wastewater by these biological treatments, which can actually eliminate total phosphorus. Through these modes of treatment we try to remove the maximum of organic matter and phosphorus in the wastewater. Consequently, the receiving environment is preserved.


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