scholarly journals Theoretical insights into the antiradical activity and copper-catalysed oxidative damage of mexidol in the physiological environment

2022 ◽  
Vol 9 (1) ◽  
Author(s):  
Nguyen Thi Hoa ◽  
Mai Van Bay ◽  
Adam Mechler ◽  
Quan V. Vo
Keyword(s):  
Oxidative Damage ◽  
Chemical Structure ◽  
Hydrogen Transfer ◽  
Therapeutic Agent ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reduction Reaction ◽  
Scavenging Activity ◽  
Hydroperoxyl Radical ◽  
Transfer Mechanisms

Mexidol ( MD , 2-ethyl-6-methyl-3-hydroxypyridine) is a registered therapeutic agent for the treatment of anxiety disorders. The chemical structure suggests that MD may also act as an antioxidant. In this study, the hydroperoxyl radical scavenging activity of MD was studied to establish baseline antioxidant activity, followed by an investigation of the effect of MD on the copper-catalysed oxidative damage in biological systems, using computational methods. It was found that MD exhibits moderate radical scavenging activity against HOO • in water and pentyl ethanoate solvents following the single electron transfer and formal hydrogen transfer mechanisms, respectively. MD can chelate Cu(II), forming complexes that are much harder to reduce than free Cu(II): MD chelation completely quenches the Cu(II) reduction by ascorbic acid and suppresses the rate of reduction reaction by O 2 ⋅ − that are the main reductants of Cu(II) in biological environments. Therefore, MD exerts its anti-HO • activity primarily as an OIL-1 inhibitor.

Comparison of the antioxidant properties of emu oil with other avian oils

2008 ◽  
Vol 48 (10) ◽  
pp. 1345 ◽  
Author(s):  
Darin C. Bennett ◽  
William E. Code ◽  
David V. Godin ◽  
Kimberly M. Cheng
Keyword(s):  
Oxidative Damage ◽  
Radical Scavenging Activity ◽  
Biological Membrane ◽  
Saturated Fatty Acids ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Thiobarbituric Acid Reactive Substance ◽  
Scavenging Activity ◽  
Triglyceride Fraction ◽  
Emu Oil

The antioxidant properties of emu oil were compared with oils derived from the fat of other avian species. We first examined their free radical scavenging activity against the 2,2-diphenyl-1-picryl hydracyl radical. The concentration of emu oil in the test solution that caused 50% neutralisation (IC50) was variable (24.5 ± 5.9 mg/mL, range 5.3–55.4 mg/mL), but similar to values obtained for other ratites (10.7 ± 5.9 mg/mL). In contrast, the IC50 values for duck and chicken oil were much higher (118.0 ± 8.1 mg/mL). The variability in the radical scavenging activity of emu oil preparations may reflect variations in the diets of the birds, the processing protocol and/or the storage age of the oil. We also evaluated some of the ratite oils for their inhibitory capacity on human erythrocyte membrane oxidation, by measuring the reduction of the thiobarbituric acid-reactive substance (TBAR) production. Emu oil had a greater effect in decreasing TBAR production than either the ostrich or rhea oil, suggesting that it offers more protection than the other ratite oils against oxidative damage. In conclusion, we demonstrated that emu oil has both antioxidant properties in vitro and a protective role against oxidative damage in a model biological membrane system. The antioxidant or radical scavenging properties of emu oil appear to be due to minor constituents in the non-triglyceride fraction of the oil, while its high ratio of unsaturated to saturated fatty acids (UFA : SFA) offers protection against oxidative damage.

scholarly journals Cytoprotective and Anti-genotoxic Effects of Xanthone Derivatives from Garcinia mangostana Against H2O2 Induced PBMC Cell and Blood Leukocytes Damage of Normal and Type 2 Diabetes Volunteers

2018 ◽  
Vol 16 (3) ◽  
pp. 143-153 ◽  
Author(s):  
Naymul KARIM ◽  
Lanchakon CHANUDOM ◽  
Jitbanjong TANGPONG
Keyword(s):  
Free Radical ◽  
Oxidative Damage ◽  
Culture Medium ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Scavenging Activity ◽  
Garcinia Mangostana ◽  
Blood Leukocytes ◽  
Xanthone Derivatives

Hyperglycemia is well-known for inducing cellular oxidative damage in type II diabetes (T2D) patients. This research addressed the cytoprotective and anti-genotoxic effect of xanthone derivatives from Garcinia mangostana against hydrogen peroxide (H2O2)-induced human peripheral blood mononuclear cell (PBMC) and blood leukocytes damage of the normal and T2D volunteers. The cytoprotective effects of an aqueous extract of xanthone (100 and 200 µg/mL) was assessed on cell viability and free radical scavenging activity using the trypan blue exclusion method on PBMC cells. Malondialdehyde (MDA) levels and lactate dehydrogenase (LDH) activity were measured as cellular oxidative damage markers and estimated from culture medium of PBMCs of normal and T2D volunteers. The anti-genotoxicity was assessed as the protective effect of xanthone against H2O2-induce DNA damage of blood leukocytes of the normal volunteers following comet assay technique. Xanthone and Gallic acid (control) concentrations 100, 200 and 100 µg/mL significantly (P < 0.05) protected from H2O2 (20 mM)-induced oxidative damage of PBMCs. It was confirmed by increased cell viability and free radical scavenging activity coupled with the decreased MDA and LDH levels in cell culture medium compared to H2O2 (20 mM)-treated group. In H2O2 (40 mM)-induced blood leukocytes of normal volunteers, different concentration xanthone (50 - 500 µg/mL) significantly (P < 0.05) improved the anti-genotoxicity effect compared to negative/positive control group by lowering comet formation. Xanthone treatments on PBMCs and blood leukocytes of the normal and T2D volunteers could attenuate the H2O2-induced cellular oxidative damage and cell death via exhibiting antioxidant and free radical scavenging activities.

scholarly journals Collagen Peptides from Swim Bladders of Giant Croaker (Nibea japonica) and Their Protective Effects against H2O2-Induced Oxidative Damage toward Human Umbilical Vein Endothelial Cells

Marine Drugs ◽  
2020 ◽  
Vol 18 (8) ◽  
pp. 430
Author(s):  
Jiawen Zheng ◽  
Xiaoxiao Tian ◽  
Baogui Xu ◽  
Falei Yuan ◽  
Jianfang Gong ◽  
...  
Keyword(s):  
Oxidative Damage ◽  
Umbilical Vein ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Extraction Process ◽  
Enzyme Concentration ◽  
Scavenging Activity ◽  
Protective Effects ◽  
Collagen Peptides ◽  
Collagen Peptide

Five different proteases were used to hydrolyze the swim bladders of Nibea japonica and the hydrolysate treated by neutrase (collagen peptide named SNNHs) showed the highest DPPH radical scavenging activity. The extraction process of SNNHs was optimized by response surface methodology, and the optimal conditions were as follows: a temperature of 47.2 °C, a pH of 7.3 and an enzyme concentration of 1100 U/g, which resulted in the maximum DPPH clearance rate of 95.44%. Peptides with a Mw of less than 1 kDa (SNNH-1) were obtained by ultrafiltration, and exhibited good scavenging activity for hydroxyl radicals, ABTS radicals and superoxide anion radicals. Furthermore, SNNH-1 significantly promoted the proliferation of HUVECs, and the protective effect of SNNH-1 against oxidative damage of H2O2-induced HUVECs was investigated. The results indicated that all groups receiving SNNH-1 pretreatment showed an increase in GSH-Px, SOD, and CAT activities compared with the model group. In addition, SNNH-1 pretreatment reduced the levels of ROS and MDA in HUVECs with H2O2-induced oxidative damage. These results indicate that collagen peptides from swim bladders of Nibea japonica can significantly reduce the oxidative stress damage caused by H2O2 in HUVECs and provides a basis for the application of collagen peptides in the food industry, pharmaceuticals, and cosmetics.

scholarly journals Enzymatic Degradation of Gracilariopsis lemaneiformis Polysaccharide and the Antioxidant Activity of Its Degradation Products

Marine Drugs ◽  
2021 ◽  
Vol 19 (5) ◽  
pp. 270
Author(s):  
Tian Fang ◽  
Xiaoqian Zhang ◽  
Shanshan Hu ◽  
Yanyan Yu ◽  
Xue Sun ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Oxidative Damage ◽  
Antioxidant Activities ◽  
Degradation Products ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Fetal Lung ◽  
Gracilariopsis Lemaneiformis ◽  
Scavenging Activity ◽  
Mitochondria Membrane Potential

Gracilariopsis lemaneiformis polysaccharides (GLP) were degraded using pectinase, glucoamylase, cellulase, xylanase, and β-dextranase into low-molecular-weight polysaccharides, namely, GPP, GGP, GCP, GXP, and GDP, respectively, and their antioxidant capacities were investigated. The degraded GLPs showed higher antioxidant activities than natural GLP, and GDP exhibited the highest antioxidant activity. After the optimization of degradation conditions through single-factor and orthogonal optimization experiments, four polysaccharide fractions (GDP1, GDP2, GDP3, and GDP4) with high antioxidant abilities (hydroxyl radical scavenging activity, DPPH radical scavenging activity, reduction capacity, and total antioxidant capacity) were obtained. Their cytoprotective activities against H2O2-induced oxidative damage in human fetal lung fibroblast 1 (HFL1) cells were examined. Results suggested that GDP pretreatment can significantly improve cell viability, reduce reactive oxygen species and malonaldehyde levels, improve antioxidant enzyme activity and mitochondria membrane potential, and alleviate oxidative damage in HFL1 cells. Thus, the enzyme degradation of GLP with β-dextranase can significantly improve its antioxidant activity, and GDP might be a suitable source of natural antioxidants.

Insights into the mechanisms and kinetics of the hydroperoxyl radical scavenging activity of Artepillin C

2021 ◽  
Author(s):  
Houssem Boulebd ◽  
Adam Mechler ◽  
Nguyen Thi Hoa ◽  
Pham Cam Nam ◽  
Duong Tuan Quang ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Single Electron ◽  
Scavenging Activity ◽  
Single Electron Transfer ◽  
Hydroperoxyl Radical ◽  
Electron Transfer Mechanism ◽  
Artepillin C ◽  
Kinetics Of

Through the single electron transfer mechanism, Artepillin C scavenges the hydroperoxyl radical in water approximately 572 times faster than Trolox.

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