The comparative power of alcohol, ether, and chloroform as measured by their action upon isolated muscle
The object of the following communication is twofold: (1) to present the results of a careful comparison of the physiological effectiveness of certain narcotics, and (2) to illustrate the degree of accuracy of which such comparisons are susceptible by the systematic use of the sartorius muscle of the frog as an indicator. Method .—The two sartorius muscles of a frog are dissected out and the portions of bone to which they are attached are ligatured with fine copper wires serving as conductors. The muscles are set up in the two vessels V, V and connected with two myographic levers that record their movements on two smoked plates L, R. The connections with the secondary coil of an inductorium (Berne model) are as given in the diagram, so that both muscles are traversed in series by the same current in the same direction. The muscles are directly excited once every 10 seconds by maximal break induction shocks. Each observation consists of three parts: a first part consisting of the normal responses of the muscle immersed in normal saline (0·6 per 100 NaCl in tap water); a second part consisting of the responses while the muscle is immersed in an experimental solution; a third part consisting of the responses while the muscle is replaced in normal saline. The solutions are changed by being run off through a tap and run in from a pipette, care being taken that the volume of fluid is always the same. The induction currents are kept going automatically throughout an experiment, excepting during the short periods required for changing the solution. The apparatus used for this purpose consists of: (1) a Berne coil fed by a 2-volt accumulator; (2) a Brodie clock with interruptions set at six per minute; and (3) a relay key, i. e. that shown by G. R. Mines at the July, 1908, meeting of the Physiological Society.