scholarly journals Determination of growth stages and metabolic profiles in Brachypodium distachyon for comparison of developmental context with Triticeae crops

2015 ◽  
Vol 282 (1811) ◽  
pp. 20150964 ◽  
Author(s):  
Yoshihiko Onda ◽  
Kei Hashimoto ◽  
Takuhiro Yoshida ◽  
Tetsuya Sakurai ◽  
Yuji Sawada ◽  
...  
Keyword(s):  
Life Cycle ◽  
Knowledge Integration ◽  
Brachypodium Distachyon ◽  
Chemical Properties ◽  
Stress Conditions ◽  
Growth Stages ◽  
Metabolome Analysis ◽  
Developmental Context ◽  
Chinese Spring Wheat ◽  
Model Grass

Brachypodium distachyon is an emerging model plant for studying biological phenomena in temperate grasses. Study of the growth scale is essential to analyse spatio-temporal changes in molecular factors throughout the life cycle. For sensitive and robust staging based on morphology in B. distachyon , we demonstrated the utility of the BBCH (Biologische Bundesanstalt, Bundessortenamt and CHemical industry) scale, which is comparable to the Zadoks scale conventionally used for Triticeae crops. We compared the chronological progression of B. distachyon accessions Bd21 and Bd3-1, in addition to the progression of Chinese Spring wheat. The comparison of growth stages illustrates the morphological similarities and differences in the timing of life cycle events. Furthermore, we compared metabolite accumulation patterns across different growth stages and across different stress conditions using a widely targeted metabolome analysis. Metabolic profiling determined commonalities and specificities in chemical properties that were dependent on organisms, growth stages and/or stress conditions. Most metabolites accumulated equivalently in B. distachyon and wheat. This qualitative similarity indicated the superiority of B. distachyon as a model for Triticeae crops. The growth scale of B. distachyon should provide a conceptual framework for comparative analysis and for knowledge integration between this model grass and crops in the Pooideae subfamily.

QTLs for resistance to the false brome rust Puccinia brachypodii in the model grass Brachypodium distachyon L.

Genome ◽  
2012 ◽  
Vol 55 (2) ◽  
pp. 152-163 ◽  
Author(s):  
Mirko Barbieri ◽  
Thierry C. Marcel ◽  
Rients E. Niks ◽  
Enrico Francia ◽  
Marianna Pasquariello ◽  
...  
Keyword(s):  
Quantitative Resistance ◽  
Rust Fungus ◽  
Brachypodium Distachyon ◽  
Transgressive Segregation ◽  
Snp Markers ◽  
Growth Stages ◽  
Resistance Qtls ◽  
Ssr And Snp Markers ◽  
Model Grass ◽  
Genomic Regions

The potential of the model grass Brachypodium distachyon L. (Brachypodium) for studying grass–pathogen interactions is still underexploited. We aimed to identify genomic regions in Brachypodium associated with quantitative resistance to the false brome rust fungus Puccinia brachypodii . The inbred lines Bd3-1 and Bd1-1, differing in their level of resistance to P. brachypodii, were crossed to develop an F2 population. This was evaluated for reaction to a virulent isolate of P. brachypodii at both the seedling and advanced growth stages. To validate the results obtained on the F2, resistance was quantified in F2-derived F3 families in two experiments. Disease evaluations showed quantitative and transgressive segregation for resistance. A new AFLP-based Brachypodium linkage map consisting of 203 loci and spanning 812 cM was developed and anchored to the genome sequence with SSR and SNP markers. Three false brome rust resistance QTLs were identified on chromosomes 2, 3, and 4, and they were detected across experiments. This study is the first quantitative trait analysis in Brachypodium. Resistance to P. brachypodii was governed by a few QTLs: two acting at the seedling stage and one acting at both seedling and advanced growth stages. The results obtained offer perspectives to elucidate the molecular basis of quantitative resistance to rust fungi.

scholarly journals Genetic Variation for Seed Metabolite Levels in Brachypodium distachyon

2019 ◽  
Vol 20 (9) ◽  
pp. 2348 ◽  
Author(s):  
Yoshihiko Onda ◽  
Komaki Inoue ◽  
Yuji Sawada ◽  
Minami Shimizu ◽  
Kotaro Takahagi ◽  
...  
Keyword(s):  
Population Structure ◽  
Vitamin B6 ◽  
Genetic Factors ◽  
Brachypodium Distachyon ◽  
Inbred Lines ◽  
Genetic Dissection ◽  
Metabolome Analysis ◽  
Ril Population ◽  
Model Grass ◽  
Metabolite Qtl

Metabolite composition and concentrations in seed grains are important traits of cereals. To identify the variation in the seed metabolotypes of a model grass, namely Brachypodium distachyon, we applied a widely targeted metabolome analysis to forty inbred lines of B. distachyon and examined the accumulation patterns of 183 compounds in the seeds. By comparing the metabolotypes with the population structure of these lines, we found signature metabolites that represent different accumulation patterns for each of the three B. distachyon subpopulations. Moreover, we found that thirty-seven metabolites exhibited significant differences in their accumulation between the lines Bd21 and Bd3-1. Using a recombinant inbred line (RIL) population from a cross between Bd3-1 and Bd21, we identified the quantitative trait loci (QTLs) linked with this variation in the accumulation of thirteen metabolites. Our metabolite QTL analysis illustrated that different genetic factors may presumably regulate the accumulation of 4-pyridoxate and pyridoxamine in vitamin B6 metabolism. Moreover, we found two QTLs on chromosomes 1 and 4 that affect the accumulation of an anthocyanin, chrysanthemin. These QTLs genetically interacted to regulate the accumulation of this compound. This study demonstrates the potential for metabolite QTL mapping in B. distachyon and provides new insights into the genetic dissection of metabolomic traits in temperate grasses.

scholarly journals Grain dormancy and light quality effects on germination in the model grass Brachypodium distachyon

2011 ◽  
Vol 193 (2) ◽  
pp. 376-386 ◽  
Author(s):  
José M. Barrero ◽  
John V. Jacobsen ◽  
Mark J. Talbot ◽  
Rosemary G. White ◽  
Stephen M. Swain ◽  
...  
Keyword(s):  
Light Quality ◽  
Brachypodium Distachyon ◽  
Grain Dormancy ◽  
Model Grass

Phenological growth stages of Brachypodium distachyon: codification and description

Weed Research ◽  
2011 ◽  
Vol 51 (6) ◽  
pp. 612-620 ◽  
Author(s):  
S-Y HONG ◽  
J-H PARK ◽  
S-H CHO ◽  
M-S YANG ◽  
C-M PARK
Keyword(s):  
Brachypodium Distachyon ◽  
Growth Stages

scholarly journals Methionine-induced regulation of growth, secondary metabolites and oxidative defense system in sunflower (Helianthus annuus L.) plants subjected to water deficit stress

PLoS ONE ◽  
2021 ◽  
Vol 16 (12) ◽  
pp. e0259585
Author(s):  
Gull Mehak ◽  
Nudrat Aisha Akram ◽  
Muhammad Ashraf ◽  
Prashant Kaushik ◽  
Mohamed A. El-Sheikh ◽  
...  
Keyword(s):  
Drought Stress ◽  
Helianthus Annuus ◽  
Water Deficit ◽  
Soluble Sugars ◽  
Foliar Spray ◽  
Shoot Length ◽  
Stress Conditions ◽  
Growth And Yield ◽  
Water Deficit Stress ◽  
Growth Stages

Optimum water availability at different growth stages is one the major prerequisites of best growth and yield production of plants. Exogenous application of plant growth regulators considered effective for normal functioning of plants under water-deficit conditions. A study was conducted to examine the influence of exogenously applied L-methionine on sunflower (Helianthus annuus L.) plants grown under water-deficit conditions. Twenty-five-day old seedlings of four sunflower cultivars, FH331, FH572, FH652 and FH623 were exposed to control (100% F.C.) and drought stress (60% F.C.) conditions. After 30-day of drought stress, L-methionine (Met; 20 mg/L) was applied as a foliar spray to control and drought stressed plants. Water deficit stress significantly reduced shoot fresh and dry weights shoot and root lengths, and chlorophyll a content in all four cultivars. While a significant increase was observed due to water deficiency in relative membrane permeability (RMP), malondialdehyde (MDA), total soluble proteins (TSP), total soluble sugars (TSS), ascorbic acid (AsA) and activity of peroxidase (POD). Although, exogenously applied Met was effective in decreasing RMP, MDA and H2O2 contents, it increased the shoot fresh weight, shoot length, chlorophyll a, chlorophyll a/b ratio, proline contents and the activities of SOD, POD and CAT enzymes in all four cultivars under water deficit stress. No change in AsA and total phenolics was observed due to foliar-applied Met under water stress conditions. Of all sunflower cultivars, cv. FH-572 was the highest and cv. FH-652 the lowest of all four cultivars in shoot fresh and dry weights as well as shoot length under drought stress conditions. Overall, foliar applied L-methionine was effective in improving the drought stress tolerance of sunflower plants that was found to be positively associated with Met induced improved growth attributes and reduced RMP, MDA and H2O2 contents under water deficit conditions.

Tightening the Life-Cycle Construct: A Taxonomic Study of Growth Stage Configurations in High-Technology Organizations

1994 ◽  
Vol 18 (2) ◽  
pp. 5-29 ◽  
Author(s):  
Steven H. Hanks ◽  
Collin J. Watson ◽  
Erik Jansen ◽  
Gaylen N. Chandler
Keyword(s):  
Cluster Analysis ◽  
Life Cycle ◽  
Growth Stage ◽  
High Technology ◽  
Life Cycle Stage ◽  
Growth Stages ◽  
Taxonomic Study ◽  
Organization Context ◽  
Organization Life Cycle

Over the years much has been written about the organization life cycle, yet there has been remarkably little attention given to the underlying construct of a life-cycle stage. It is proposed in this study that each life-cycle stage consists of a unique configuration of variables related to organization context and structure. Cluster analysis is used to derive a taxonomy of growth stage configurations in a sample of 126 high-technology organizations. The derived configurations suggest a sequence of four growth stages.

Maturity in Outsourcing Relationships

2010 ◽  
pp. 98-117
Author(s):  
Hans Solli-Sæther ◽  
Petter Gottschalk
Keyword(s):  
Life Cycle ◽  
Information Systems ◽  
Product Life Cycle ◽  
Growth Models ◽  
It Outsourcing ◽  
Growth Stages ◽  
Stage Model ◽  
Stages Of Growth ◽  
Systems Planning ◽  
Living Organisms

Stages of growth models have been used widely in both organizational research and information technology management research. According to King and Teo (1997), these models describe a wide variety of phenomena – the organizational life cycle, product life cycle, biological growth, stages of growth in information systems, growth model for integration between business planning and information systems planning, electronic commerce evolution, stages of knowledge management technology, and a number of other interesting developments in time perspectives. These models assume that predictable patterns (conceptualized in terms of stages or levels) exist in the growth of organizations and organizational parts, the sales levels of products, and the growth of living organisms. These stages are (1) sequential in nature, (2) occur as a hierarchical progression that is not easily reversed, and (3) evolve a broad range of organizational activities and structures. This chapter starts with an introduction to stages of growth models. In the following sections we present the three-stage model for the evolution of IT outsourcing relationships (Gottschalk & Solli-Sæther, 2006). The three stages are labelled cost stage, resource stage, and partnership stage respectively. Theory-based benchmark variables for measuring maturity in IT outsourcing relationships are presented, followed by the stage hypothesis and a description of how benchmark variables are used to indicate characteristics at each stage of growth. Finally in this chapter, we present results from an exploratory study testing the stage model. The purpose of this chapter is to develop a framework for improved understanding of the current situation in an IT outsourcing relationship in terms of a specific stage, to develop strategies for moving to a higher stage in the future, and to learn from earlier stage experience.

scholarly journals Centromeric DNA characterization in the model grass Brachypodium distachyon provides insights on the evolution of the genus

2018 ◽  
Vol 93 (6) ◽  
pp. 1088-1101 ◽  
Author(s):  
Yinjia Li ◽  
Sheng Zuo ◽  
Zhiliang Zhang ◽  
Zhanjie Li ◽  
Jinlei Han ◽  
...  
Keyword(s):  
Brachypodium Distachyon ◽  
Centromeric Dna ◽  
Dna Characterization ◽  
Model Grass

scholarly journals A novel transcription factor-like gene SbSDR1 acts as a molecular switch and confers salt and osmotic endurance to transgenic tobacco

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Vijay Kumar Singh ◽  
Avinash Mishra ◽  
Intesaful Haque ◽  
Bhavanath Jha
Keyword(s):  
Transcription Factor ◽  
Transgenic Plants ◽  
Soluble Sugars ◽  
Membrane Integrity ◽  
Molecular Switch ◽  
Stress Conditions ◽  
Growth And Yield ◽  
Physiological Status ◽  
Growth Stages ◽  
Potential Candidate

Abstract A salt- and drought-responsive novel gene SbSDR1 is predominantly localised to the nucleus, up-regulated under abiotic stresses and is involved in the regulation of metabolic processes. SbSDR1 showed DNA-binding activity to genomic DNA, microarray analysis revealed the upregulation of host stress-responsive genes and the results suggest that SbSDR1 acts as a transcription factor. Overexpression of SbSDR1 did not affect the growth and yield of transgenic plants in non-stress conditions. Moreover, the overexpression of SbSDR1 stimulates the growth of plants and enhances their physiological status by modulating the physiology and inhibiting the accumulation of reactive oxygen species under salt and osmotic stress. Transgenic plants that overexpressed SbSDR1 had a higher relative water content, membrane integrity and concentration of proline and total soluble sugars, whereas they showed less electrolyte leakage and lipid peroxidation than wild type plants under stress conditions. In field conditions, SbSDR1 plants recovered from stress-induced injuries and could complete their life cycle. This study suggests that SbSDR1 functions as a molecular switch and contributes to salt and osmotic tolerance at different growth stages. Overall, SbSDR1 is a potential candidate to be used for engineering salt and drought tolerance in crops without adverse effects on growth and yield.

Sign in / Sign up

Export Citation Format

Share Document