The application of coenzyme-dependent enzymes in biotechnology

1983 ◽  
Vol 300 (1100) ◽  
pp. 335-353 ◽  
Keyword(s):  
Dual Problem ◽  
Immobilized Enzyme ◽  
Specific Activity ◽  
Biological Systems ◽  
Covalent Immobilization ◽  
Future Prospects ◽  
The Future

The principal problems associated with the application of immobilized coenzymcdependent enzymes in biotechnology are discussed. Particular emphasis is laid on the problems encountered in the covalent immobilization of the nicotinamide nucleotide oxidoreductases and on the special problems posed by the freely dissociable coenzyme. Thus the influence of the immobilization regime on the specific activity and stability of such enzymes and the techniques available for the immobilization, retention and regeneration of the coenzyme moiety are discussed. The solution of the dual problem of retention and regeneration by co-immobilized enzyme-coenzyme systems and the applications of enzyme-coenzyme systems in industry, medicine and analysis are also given. Finally, this report speculates on the future prospects for enzyme-coenzyme systems in biotechnology, how some of the problems may be resolved and how, in some cases, quasi-biological or non-biological systems may represent useful alternatives.

scholarly journals Understanding Hydrogen Sulfide in Inflammation: Opportunities and Challenges

2019 ◽  
Author(s):  
Madhav Bhatia
Keyword(s):  
Hydrogen Sulfide ◽  
Tissue Damage ◽  
Adaptive Response ◽  
Inflammatory Diseases ◽  
Biological Systems ◽  
Future Prospects ◽  
Disease Research ◽  
The Future

Inflammation is an adaptive response to injury, but uncontrolled inflammation can lead to tissue damage and disease. Research in our laboratory (since confirmed in different laboratories worldwide) has shown that hydrogen sulfide (H2S) acts as a mediator of inflammation in different disease conditions. Learning about a novel mediator of inflammation results in unique opportunities with which to approach inflammatory diseases. At the same time, the complexity of biological systems and translation of research from the bed to the bedside also presents challenges. This Editorial aims to discuss the opportunities and challenges in relation to the role of H2S in inflammation, and the future prospects for this research.

Freeze-fracture cytochemistry: A goal set by Russell Steere in 1957

1993 ◽  
Vol 51 ◽  
pp. 60-61
Author(s):  
Nicholas J Severs
Keyword(s):  
Chemical Nature ◽  
Biological Systems ◽  
Freeze Fracture ◽  
Structural Components ◽  
Major Goal ◽  
Membrane Biology ◽  
Routine Application ◽  
The Future ◽  
Freeze Etching

In his pioneering demonstration of the potential of freeze-etching in biological systems, Russell Steere assessed the future promise and limitations of the technique with remarkable foresight. Item 2 in his list of inherent difficulties as they then stood stated “The chemical nature of the objects seen in the replica cannot be determined”. This defined a major goal for practitioners of freeze-fracture which, for more than a decade, seemed unattainable. It was not until the introduction of the label-fracture-etch technique in the early 1970s that the mould was broken, and not until the following decade that the full scope of modern freeze-fracture cytochemistry took shape. The culmination of these developments in the 1990s now equips the researcher with a set of effective techniques for routine application in cell and membrane biology.Freeze-fracture cytochemical techniques are all designed to provide information on the chemical nature of structural components revealed by freeze-fracture, but differ in how this is achieved, in precisely what type of information is obtained, and in which types of specimen can be studied.

On the Preparation of Bovine α-Thrombin

1978 ◽  
Vol 39 (01) ◽  
pp. 193-200 ◽  
Author(s):  
Erwin F Workman ◽  
Roger L Lundblad
Keyword(s):  
Immobilized Enzyme ◽  
Catalytic Properties ◽  
Specific Activity ◽  
Guanidine Hydrochloride ◽  
Low Molecular Weight ◽  
Reversible Process ◽  
Gel Beads ◽  
Tissue Thromboplastin ◽  
C 50 ◽  
Hydrolysis Of

SummaryAn improved method for the preparation of bovine α-thrombin is described. The procedure involves the activation of partially purified prothrombin with tissue thromboplastin followed by chromatography on Sulfopropyl-Sephadex C-50. The purified enzyme is homogeneous on polyacrylamide discontinuous gel electrophoresis and has a specific activity toward fibrinogen of 2,200–2,700 N.I.H. U/mg. Its stability on storage in liquid media is dependent on both ionic strenght and temperature. Increasing ionic strength and decreasing temperature result in optimal stability. The denaturation of α-thrombin by guanidine hydrochloride was found to be a partially reversible process with the renatured species possessing properties similar to “aged” thrombin. In addition, the catalytic properties of a-thrombin covalently attached to agarose gel beads were also examined. The activity of the immobilized enzyme toward fibrinogen was affected to a much greater extent than was the hydrolysis of low molecular weight, synthetic substrates.

Deadlock or new opening? Current state and prospects for development of Lithuanian-Belarusian relations

2021 ◽  
Vol 19 (3) ◽  
pp. 121-141
Author(s):  
Justyna Olędzka
Keyword(s):  
International Relations ◽  
Presidential Election ◽  
Nuclear Power ◽  
Future Prospects ◽  
Bilateral Relations ◽  
Current State ◽  
The Future ◽  
Multi Level ◽  
The Moment ◽  
The Eu

The purpose of this article is to discuss the trajectory of Belarusian-Lithuanian relations with a particular focus on the period after the 2020 Belarusian presidential election, which resulted in a change in international relations in the region. This was the moment that redefined the Lithuanian-Belarusian relations, which until 2020 were satisfactory for both sides (especially in the economic aspect). However, Lithuania began to pursue a reactive policy of promoting the democratisation of Belarus and provided multi-level support to Belarusian opposition forces. The current problems in bilateral relations (e.g., the future of Belarusian Nuclear Power Plant located in Astravyets) have been put on the agenda for discussion at the EU level, while the instruments of a hybrid conflict in the form of an influx of immigrants into Lithuania, controlled by the Belarusian regime, have become a key issue for the future prospects of relations between Belarus and Lithuania.

scholarly journals The History and the Future Prospects of a Durable High Rust Preventive

2017 ◽  
Vol 68 (1) ◽  
pp. 38-42
Author(s):  
Tsuyoshi MATSUMOTO ◽  
Tsuyoshi MIYASHITA
Keyword(s):  
Future Prospects ◽  
The Future

scholarly journals Islamophobia The New Dimensions In 21st Century

2021 ◽  
Vol 3 (2) ◽  
Author(s):  
Dr. Rani Erum
Keyword(s):  
Life History ◽  
Present State ◽  
21St Century ◽  
Future Prospects ◽  
Human Psychology ◽  
The World ◽  
The Future ◽  
Unique Approach ◽  
The Common ◽  
Harmful Effects

Anti-Muslim emotions are not new for the world. It was present since the rise of Islam. West was furious after facing Muslims in battle ground and constantly defeated by those who were less equipped but obtained high morals. Initially they were frightened due to the novelty and unique approach of faith and its execution, therefore, they try to fabricate the original manuscripts, making false stories and molesting the last prophet’s life history. Islamophobia transformed after 9/11 and become more intensified and dangerous. It effected the common men worldwide without any boundaries. The respective research is based on the fact that hatred is the negative notion whether it related with nobility or wicked perception. It creates harmful effects on human psychology which subsequently created abusive mindset and actions. When any form of ideology identified as phobia means uncontrolled envy combine with the concept and turn it into a form of frenzied connotation. The fight between East and West was ancient which now convert among religions particularly Islam and others. The research is intend to provide the journey of anti-Islamism from past to present as well its significant elements and its present state. It also discusses the future prospects of clash of religions.  

scholarly journals Covalent Immobilization of Thiol Proteinases on Chitosan

2020 ◽  
Vol 2 (1) ◽  
pp. 7
Author(s):  
Svetlana Olshannikova ◽  
Victoria Koroleva ◽  
Marina Holyavka ◽  
Alexander Pashkov ◽  
Valeriy Artyukhov
Keyword(s):  
Specific Activity ◽  
Crosslinking Agent ◽  
Total Activity ◽  
Covalent Immobilization ◽  
Tropical Plants ◽  
Chitosan Matrix ◽  
Plant Enzymes ◽  
Thiol Proteases ◽  
Thiol Proteinases ◽  
Wide Substrate Specificity

Plant enzymes such as ficin (EC 3.4.22.3), papain (EC 3.4.22.2) and bromelain (EC 3.4.22.4) are obtained from tropical plants. These biocatalysts belong to thiol proteases, in the active center of which cysteine is contained. Ficin, papain and bromelain have a wide substrate specificity, which provides a demand for their use in various industries. Enzymes in the free state are less commonly used; immobilized biocatalysts are the preferred form. The aim of this work was to determine the optimal concentration of a crosslinking agent in the covalent immobilization of ficin, papain and bromelain on a chitosan matrix. Ficin, papain and bromelain (Sigma) were chosen as objects of study. An acid-soluble chitosan (350 kDa, Bioprogress CJSC) was used as an immobilization carrier. The concentration range of glutaraldehyde (crosslinking agent) ranged from 1 to 25%. Suitable concentrations of glutaraldehyde for covalent immobilization were identified by the optimal ratio of protein content (mg per g of carrier), total activity (in units per ml of solution) and specific activity (in units per mg of protein). It was shown that for covalent immobilization of ficin and bromelain on a chitosan matrix, it is most promising to use 10% glutaraldehyde. For immobilization of papain on chitosan by covalent means, the concentration of glutaraldehyde equal to 20% is optimal.

Sign in / Sign up

Export Citation Format

Share Document