scholarly journals Reclassification of Bacteroides ureolyticus as Campylobacter ureolyticus comb. nov., and emended description of the genus Campylobacter

2010 ◽  
Vol 60 (9) ◽  
pp. 2016-2022 ◽  
Author(s):  
P. Vandamme ◽  
L. Debruyne ◽  
E. De Brandt ◽  
E. Falsen
Keyword(s):  
Fatty Acid ◽  
Bacterial Species ◽  
Cellular Fatty Acid ◽  
Present Species ◽  
Published Data ◽  
Respiratory Quinone ◽  
Dna Base ◽  
Emended Description ◽  
Cellular Fatty Acid Profile ◽  
Fatty Acid Compositions

The protein profiles, genomic amplified fragment length polymorphism patterns and 16S rRNA and cpn60 gene sequences of a diverse collection of 26 Bacteroides ureolyticus strains, along with published data on their DNA base, respiratory quinone and cellular fatty acid compositions, were used to reassess the taxonomy of this bacterial species. The results demonstrate that this organism is most appropriately allocated in the genus Campylobacter. The presence of much higher amounts of 18 : 1ω7c in its cellular fatty acid profile and its ability to digest gelatin and casein are the characteristics that differentiate it from present species of the genus Campylobacter. Therefore we propose to reclassify this species incertae sedis into the genus Campylobacter as Campylobacter ureolyticus with strain LMG 6451T (=CCUG 7319T =NCTC 10941T) as the type strain.

scholarly journals Prevotella copri sp. nov. and Prevotella stercorea sp. nov., isolated from human faeces

2007 ◽  
Vol 57 (5) ◽  
pp. 941-946 ◽  
Author(s):  
Hidenori Hayashi ◽  
Kensaku Shibata ◽  
Mitsuo Sakamoto ◽  
Shinichi Tomita ◽  
Yoshimi Benno
Keyword(s):  
Fatty Acid ◽  
Sequence Similarity ◽  
Cellular Fatty Acid ◽  
Rrna Gene ◽  
Fatty Acid Profiles ◽  
Phenotypic Characteristics ◽  
Human Faeces ◽  
Gene Sequence Analysis ◽  
Type Strains ◽  
Fatty Acid Compositions

Six strains (CB7T, CB18, CB23, CB26, CB28 and CB35T) were isolated from human faeces. Based on phylogenetic analysis, phenotypic characteristics, cellular fatty acid profiles and menaquinone profiles, these strains could be included within the genus Prevotella and made up two clusters. 16S rRNA gene sequence analysis indicated that five strains were most closely related to Prevotella veroralis, sharing about 92 % sequence similarity; the remaining strain was most closely related to Prevotella shahii, sharing about 90 % sequence similarity. All six strains were obligately anaerobic, non-pigmented, non-spore-forming, non-motile, Gram-negative rods. The cellular fatty acid compositions of the six strains differed significantly from those of other Prevotella species. Five strains (CB7T, CB18, CB23, CB26 and CB28) contained dimethyl acetals and the major menaquinones of these strains were MK-11, MK-12 and MK-13. The major menaquinones of CB35T were MK-12 and MK-13. Based on phenotypic and phylogenetic findings, two novel species, Prevotella copri sp. nov. and Prevotella stercorea sp. nov., are proposed, representing the two different strain clusters. The DNA G+C contents of strains CB7T and CB35T were 45.3 and 48.2 mol%, respectively. The type strains of P. copri and P. stercorea are CB7T (=JCM 13464T=DSM 18205T) and CB35T (=JCM 13469T=DSM 18206T), respectively.

Cellular fatty acid compositions of "Achromobacter groups B and E".

1993 ◽  
Vol 31 (4) ◽  
pp. 1007-1008 ◽  
Author(s):  
B Holmes ◽  
C W Moss ◽  
M I Daneshvar
Keyword(s):  
Fatty Acid ◽  
Cellular Fatty Acid ◽  
Fatty Acid Compositions

scholarly journals Catellibacterium nanjingense sp. nov., a propanil-degrading bacterium isolated from activated sludge, and emended description of the genus Catellibacterium

2012 ◽  
Vol 62 (Pt_3) ◽  
pp. 495-499 ◽  
Author(s):  
Jun Zhang ◽  
Shu-An Chen ◽  
Jin-Wei Zheng ◽  
Shu Cai ◽  
Bao-Jian Hang ◽  
...  
Keyword(s):  
Activated Sludge ◽  
Cellular Fatty Acid ◽  
Rrna Gene ◽  
Membrane Structures ◽  
Respiratory Quinone ◽  
Degrading Bacterium ◽  
Emended Description ◽  
Major Respiratory Quinone ◽  
Ubiquinone 10 ◽  
Sequence Similarities

A novel facultatively anaerobic, non-spore-forming, non-motile, catalase- and oxidase-positive, Gram-negative and rod-shaped bacterial strain, designated Y12T, was isolated from activated sludge of a wastewater bio-treatment facility. The strain was able to degrade about 90 % of added propanil (100 mg l−1) within 3 days of incubation. Growth occurred in the presence of 0–4.5 % (w/v) NaCl (optimum 0.5 %), at 10–40 °C (optimum 28 °C) and at pH 5.5–10.0 (optimum pH 7.0). Vesicular internal membrane structures and photoheterotrophic growth were not observed. The major respiratory quinone was ubiquinone-10 and the major cellular fatty acid was summed feature 8 (C18 : 1ω6c and/or C18 : 1ω7c). The genomic DNA G+C content of strain Y12T was 63.7 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparison revealed that strain Y12T was a member of the genus Catellibacterium, as it showed highest sequence similarities to Catellibacterium caeni DCA-1T (99.1 %) and <96.0 % similarities with other species of the genus Catellibacterium. Strain Y12T showed low DNA–DNA relatedness values with C. caeni DCA-1T. Based on phenotypic, genotypic and phylogenetic properties, strain Y12T represents a novel species of the genus Catellibacterium, for which the name Catellibacterium nanjingense sp. nov. is proposed. The type strain is Y12T ( = CCTCC AB 2010218T  = KCTC 23298T). An emended description of the genus Catellibacterium is also presented.

scholarly journals Fluorescent pseudomonads capable of growth at 41 degrees C but distinct from Pseudomonas aeruginosa

1976 ◽  
Vol 4 (5) ◽  
pp. 443-449
Author(s):  
G W Ajello ◽  
A W Hoadley
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Fatty Acid ◽  
Carbon Source ◽  
Antibiotic Susceptibility ◽  
Sole Carbon Source ◽  
Cellular Fatty Acid ◽  
Fluorescent Pseudomonads ◽  
Nitrogen Gas ◽  
Fluorescent Pseudomonas ◽  
Fatty Acid Compositions

One hundred and twenty-seven apyocyanogenic fluorescent Pseudomonas strains capable of growth at 41 degrees C, but differing from Pseudomonas aeruginosa, were typed serologically and tested for pyocin production, antibiotic susceptibility, selected biochemical reactions, and utilization of selected substrates. Results were compared with those from 40 apyocyanogenic and 14 pyocyanin-producing strains of P. aeruginosa. Unidentified fluorescent Pseudomonas (UFP) strains generally were not agglutinated by P. aeruginosa antisera and showed little or no pyocin activity. In contrast to P. aeruginosa strains, UFP strains usually failed to oxidize D-gluconate or reduce nitrate to nitrogen gas. They could not use D-gluconate or D-mannitol as sole carbon source and were susceptible to kanamycin. The cellular fatty acid compositions of major UFP groups resembled those of the alcaligenes-stutzeri groups.

Proposal that the arsenite-oxidizing organisms Thiomonas cuprina and ‘Thiomonas arsenivorans’ be reclassified as strains of Thiomonas delicata, and emended description of Thiomonas delicata

2011 ◽  
Vol 61 (12) ◽  
pp. 2816-2821 ◽  
Author(s):  
Fabienne Battaglia-Brunet ◽  
Hafida El Achbouni ◽  
Marianne Quemeneur ◽  
Kevin B. Hallberg ◽  
Donovan P. Kelly ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Sequence Similarity ◽  
Bacterial Species ◽  
Single Species ◽  
Threshold Value ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Mining Sites ◽  
Emended Description ◽  
Definition Of

The three As(III)-oxidizing members of the class Betaproteobacteria Thiomonas delicata, Thiomonas cuprina and ‘Thiomonas arsenivorans’ were isolated from mining sites in geographically distinct areas, namely Japan, Germany and France, respectively. They are all able to oxidize As(III) but only ‘T. arsenivorans’ and T. cuprina show efficient autotrophic growth with As(III) and are able to grow on a sole carbon source. These two organisms are also motile, whereas T. delicata is not. Only T. cuprina can grow autotrophically on chalcopyrite. The three strains share >99 % gene sequence similarity with each other based on their 16S rRNA genes and 16S–23S ITS regions. DNA–DNA hybridization results are above, or close to, the threshold value of 70 % recommended for the definition of bacterial species. The three taxa show very similar fatty acid profiles with differences only in five minor fatty acid components. They possess phylogenetic and chemotaxonomic similarities supporting the reclassification of these taxa as a single species. We propose that ‘T. arsenivorans’ and T. cuprina be reassigned as strains of T. delicata (type strain DSM 17897T).

Effect of wastewater composition on microbial populations in biological phosphorus removal processes

1998 ◽  
Vol 38 (1) ◽  
pp. 159-166 ◽  
Author(s):  
J. C. Wang ◽  
J. K. Park
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Profile ◽  
Phosphorus Removal ◽  
Microbial Population ◽  
Profile Analysis ◽  
Cellular Fatty Acid ◽  
Phosphorus Concentration ◽  
Biological Phosphorus Removal ◽  
Biological Phosphorus ◽  
Cellular Fatty Acid Profile

Bench-scale sequential batch reactors (SBRs) were fed with glucose- and acetate-containing synthetic wastewaters to evaluate microbial population dynamics and types of phosphorus-accumulating organisms (PAOs) using a cellular fatty acid profile analysis. The phosphorus content in the sludge was 38% (w/w) for the acetate-fed SBR and 20% (w/w) for the glucose-fed SBR with a VSS/TSS ratio of 50%. Glucose-fed PAOs were found to remove phosphorus with accumulation of glycogen in cells without synthesizing poly-β-hydroxybutyrate (PHB) at influent phosphorus concentration &lt; 20 mg-P/L and nitrate concentration &lt; 2 mg-N/L. From the fatty acid profile biomarker study, it was found that the glucose-fed SBR maintained the same fatty acid profile before and after biological phosphorus removal (BPR) occurred while the acetate-fed SBR had a different fatty acid profile. The microbial population in the glucose-fed SBR was significantly different in terms of metabolic behavior and cellular fatty acid profile from that introduced in the acetate-fed SBR. Fatty acid a15:0 (anteiso methyl-branching) was abundant in the glucose-fed PAOs. Among the five PAO candidates (Acinetobacter, Pseudomonas, Arthrobacter, Aeromonas, and Micrococcus), only Arthrobacter spp. had the biomarker of fatty acid a15:0, indicating that Arthrobacter spp. may be one of the PAOs existing in the glucose-fed bioreactors.

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