Tuwongella immobilis gen. nov., sp. nov., a novel non-motile bacterium within the phylum Planctomycetes

Planctomycetes are bacteria that were long thought to be unculturable, of low abundance, and therefore neglectable in the environment. This view changed in recent years, after it was shown that members of the phylum Planctomycetes can be abundant in many aquatic environments, e.g., in the epiphytic communities on macroalgae surfaces. Here, we analyzed three different macroalgae from the North Sea and show that Planctomycetes is the most abundant bacterial phylum on the alga Fucus sp., while it represents a minor fraction of the surface-associated bacterial community of Ulva sp. and Laminaria sp. Especially dominant within the phylum Planctomycetes were Blastopirellula sp., followed by Rhodopirellula sp., Rubripirellula sp., as well as other Pirellulaceae and Lacipirellulaceae, but also members of the OM190 lineage. Motivated by the observed abundance, we isolated four novel planctomycetal strains to expand the collection of species available as axenic cultures since access to different strains is a prerequisite to investigate the success of planctomycetes in marine environments. The isolated strains constitute four novel species belonging to one novel and three previously described genera in the order Pirellulales, class Planctomycetia, phylum Planctomycetes.

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Gliding Motility Revisited: How Do the Myxobacteria Move without Flagella?

Microbiology and Molecular Biology Reviews ◽

10.1128/mmbr.00043-09 ◽

2010 ◽

Vol 74 (2) ◽

pp. 229-249 ◽

Cited By ~ 89

Author(s):

Emilia M. F. Mauriello ◽

Tâm Mignot ◽

Zhaomin Yang ◽

David R. Zusman

Keyword(s):

Biofilm Formation ◽

Protein Localization ◽

Myxococcus Xanthus ◽

Gliding Motility ◽

Solid Surfaces ◽

Biological Functions ◽

Fruiting Body Formation ◽

Body Formation ◽

Periplasmic Flagella ◽

Motile Bacterium

SUMMARY In bacteria, motility is important for a wide variety of biological functions such as virulence, fruiting body formation, and biofilm formation. While most bacteria move by using specialized appendages, usually external or periplasmic flagella, some bacteria use other mechanisms for their movements that are less well characterized. These mechanisms do not always exhibit obvious motility structures. Myxococcus xanthus is a motile bacterium that does not produce flagella but glides slowly over solid surfaces. How M. xanthus moves has remained a puzzle that has challenged microbiologists for over 50 years. Fortunately, recent advances in the analysis of motility mutants, bioinformatics, and protein localization have revealed likely mechanisms for the two M. xanthus motility systems. These results are summarized in this review.

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Characterization of Planctomyces limnophilus and Development of Genetic Tools for Its Manipulation Establish It as a Model Species for the Phylum Planctomycetes

Applied and Environmental Microbiology ◽

10.1128/aem.05132-11 ◽

2011 ◽

Vol 77 (16) ◽

pp. 5826-5829 ◽

Cited By ~ 59

Author(s):

Christian Jogler ◽

Frank Oliver Glöckner ◽

Roberto Kolter

Keyword(s):

Eukaryotic Cells ◽

Cellular Structures ◽

Model Species ◽

Content Type ◽

Genetic Tools ◽

Carbon And Nitrogen ◽

Nitrogen Cycles ◽

Global Carbon ◽

The Phylum Planctomycetes

ABSTRACTPlanctomycetesrepresent a remarkable clade in the domainBacteriabecause they play crucial roles in global carbon and nitrogen cycles and display cellular structures that closely parallel those of eukaryotic cells. Studies onPlanctomyceteshave been hampered by the lack of genetic tools, which we developed forPlanctomyces limnophilus.

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The Polar Flagellar Motor of Vibrio cholerae Is Driven by an Na+ Motive Force

Journal of Bacteriology ◽

10.1128/jb.181.6.1927-1930.1999 ◽

1999 ◽

Vol 181 (6) ◽

pp. 1927-1930 ◽

Cited By ~ 79

Author(s):

Seiji Kojima ◽

Koichiro Yamamoto ◽

Ikuro Kawagishi ◽

Michio Homma

Keyword(s):

Energy Source ◽

Vibrio Cholerae ◽

Specific Inhibitor ◽

Motive Force ◽

Flagellar Motor ◽

Genome Sequencing Project ◽

Genome Database ◽

Sequencing Project ◽

Organelle Motility ◽

Motile Bacterium

ABSTRACT Vibrio cholerae is a highly motile bacterium which possesses a single polar flagellum as a locomotion organelle. Motility is thought to be an important factor for the virulence ofV. cholerae. The genome sequencing project of this organism is in progress, and the genes that are highly homologous to the essential genes of the Na+-driven polar flagellar motor of Vibrio alginolyticus were found in the genome database of V. cholerae. The energy source of its flagellar motor was investigated. We examined the Na+ dependence and the sensitivity to the Na+ motor-specific inhibitor of the motility of the V. cholerae strains and present the evidence that the polar flagellar motor of V. choleraeis driven by an Na+ motive force.

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Updates to the recently introduced family Lacipirellulaceae in the phylum Planctomycetes: isolation of strains belonging to the novel genera Aeoliella, Botrimarina, Pirellulimonas and Pseudobythopirellula and the novel species Bythopirellula polymerisocia and Posidoniimonas corsicana

Antonie van Leeuwenhoek ◽

10.1007/s10482-020-01486-3 ◽

2020 ◽

Vol 113 (12) ◽

pp. 1979-1997

Author(s):

Sandra Wiegand ◽

Mareike Jogler ◽

Christian Boedeker ◽

Anja Heuer ◽

Stijn H. Peeters ◽

...

Keyword(s):

Type Strain ◽

Novel Species ◽

The Novel ◽

Generation Times ◽

The Family ◽

Resource Collection ◽

The Baltic ◽

Mallorca Island ◽

Costa Brava ◽

The Phylum Planctomycetes

AbstractEight novel strains of the phylum Planctomycetes were isolated from different aquatic habitats. Among these habitats were the hydrothermal vent system close to Panarea Island, a public beach at Mallorca Island, the shore of Costa Brava (Spain), and three sites with brackish water in the Baltic Sea. The genome sizes of the novel strains range from 4.33 to 6.29 Mb with DNA G+C contents between 52.8 and 66.7%. All strains are mesophilic (Topt 24–30 °C) and display generation times between 17 and 94 h. All eight isolates constitute novel species of either already described or novel genera within the family Lacipirellulaceae. Two of the novel species, Posidoniimonas polymericola (type strain Pla123aT = DSM 103020T = LMG 29466T) and Bythopirellula polymerisocia (type strain Pla144T = DSM 104841T = VKM B-3442T), belong to established genera, while the other strains represent the novel genera Aeoliella gen. nov., Botrimarina gen. nov., Pirellulimonas gen. nov. and Pseudobythopirellula gen. nov. Based on our polyphasic analysis, we propose the species Aeoliella mucimassa sp. nov. (type strain Pan181T = DSM 29370T = LMG 31346T = CECT 9840T = VKM B-3426T), Botrimarina colliarenosi sp. nov. (type strain Pla108T = DSM 103355T = LMG 29803T), Botrimarina hoheduenensis sp. nov. (type strain Pla111T = DSM 103485T = STH00945T, Jena Microbial Resource Collection JMRC), Botrimarina mediterranea sp. nov. (type strain Spa11T = DSM 100745T = LMG 31350T = CECT 9852T = VKM B-3431T), Pirellulimonas nuda sp. nov. (type strain Pla175T = DSM 109594T = CECT 9871T = VKM B-3448T) and Pseudobythopirellula maris sp. nov. (type strain Mal64T = DSM 100832T = LMG 29020T).

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Glanders in horses in some selected areas of Bangladesh and comparison between CFT and Immunoblot used for the screening of glanders

Indian Journal of Animal Research ◽

10.18805/ijar.b-976 ◽

2018 ◽

Author(s):

Md. Siddiqur Rahman ◽

Palash Kumar Bhattacharjee ◽

Roma Rani Sarker ◽

Mst. Sonia Parvin ◽

Sayra Tasnin ◽

...

Keyword(s):

Screening Test ◽

Zoonotic Disease ◽

Field Conditions ◽

Prevalence Study ◽

Serum Samples ◽

Immunoblot Assay ◽

Gram Negative ◽

Mucous Membranes ◽

Preliminary Study ◽

Motile Bacterium

Glanders is a fatal infectious and notifiable zoonotic disease of equines caused by the Gram-negative non-motile bacterium Burkholderia (B.) mallei, which is responsible for chronic suppurative lesions of the skin and mucous membranes, pneumonia and septicemia in equines. Glanders in horses is worldwide distributed and reported from many countries. But no prevalence study was done in Bangladesh so far. Therefore, this preliminary study was conducted to determine the seroprevalence of glanders in horses using CFT and immunoblot assay. A total of 301 serum samples from horses were collected foe the detection of glanders antibodies from Mymensingh, Tangail and Jamalpur districts in Bangladesh. By CFT 105 samples were found positive and 23 samples were suspicious. The immunoblot confirmed 26 of these samples but 3 remained suspicious. The overall seroprevalence of glanders was 34.9% based on CFT and 24.8% based on immunoblot. Higher prevalence was found in Jamalpur (11.81%). CFT is considered to be a suitable screening test for the diagnosis of glanders in field conditions in Bangladesh.

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The Phylum Planctomycetes

The Prokaryotes ◽

10.1007/978-3-642-38954-2_155 ◽

2014 ◽

pp. 759-810 ◽

Cited By ~ 1

Author(s):

Noha H. Youssef ◽

Mostafa S. Elshahed

Keyword(s):

The Phylum Planctomycetes

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THE REMOVAL OF AGGLUTININ FROM SENSITIZED MOTILE BACTERIA

Journal of Experimental Medicine ◽

10.1084/jem.50.3.377 ◽

1929 ◽

Vol 50 (3) ◽

pp. 377-386 ◽

Cited By ~ 1

Author(s):

John B. Nelson

Keyword(s):

Free Water ◽

Bacterial Agglutination ◽

Motile Bacterium ◽

Motile Bacteria

The salt-free water washings of a sensitized motile bacterium (B. aertrycke) were found to cause a floccular agglutination in the presence of both whole and deflagellated antigen. Evidence was presented that the water washings when salt-free contained flagella and flagellar agglutinin and that clumping occurred upon the addition of saline. The floccular reaction in the presence of deflagellated bacteria was regarded as the agglutination of flagella present in the washings. In the presence of whole bacteria, however, actual bacterial agglutination resulted.

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