Saksenaea erythrospora infection following a serious sailing accident

2014 ◽  
Vol 63 (2) ◽  
pp. 317-321 ◽  
Author(s):  
Silvia Relloso ◽  
Vanesa Romano ◽  
Maria Fernanda Landaburu ◽  
Fabian Herrera ◽  
Jorgelina Smayevsky ◽  
...  
Keyword(s):  
Clinical Sample ◽  
Its Region ◽  
Biopsy Material ◽  
Agar Block ◽  
Fungal Identification ◽  
Direct Microscopic Examination ◽  
Deficient Medium ◽  
Tissue Contamination ◽  
Identification Of Species ◽  
Soil And Water

Saksenaea erythrospora is a species of the order Mucorales recently described and reported as a cause of human mucormycosis. We report a case of S. erythrospora in a man involved in a serious sailing accident causing deep skin and soft tissue contamination with soil and water. Direct microscopic examination of the clinical sample with Giemsa stains showed hyaline and non-septate hyphae belonging to the order Mucorales. Fungal identification was performed by culture of biopsy material on SDA, and identification of species by floating an agar block containing the fungus in a nutritionally deficient medium consisting of sterile distilled water supplemented with 0.05 % yeast extract; and by sequencing the ITS region of the rDNA. This is the first report to our knowledge of infection with S. erythrospora in Argentina, confirming the presence of this fungus in this country.

scholarly journals Development of PCR Assays for the Identification of Species and Pathotypes of Elsinoë Causing Scab on Citrus

Plant Disease ◽  
2007 ◽  
Vol 91 (7) ◽  
pp. 865-870 ◽  
Author(s):  
J. W. Hyun ◽  
N. A. Peres ◽  
S.-Y. Yi ◽  
L. W. Timmer ◽  
K. S. Kim ◽  
...  
Keyword(s):  
Internal Transcribed Spacer ◽  
Sweet Orange ◽  
Random Amplified Polymorphic Dna ◽  
Its Region ◽  
The United States ◽  
Specific Primer ◽  
Identification Of Species ◽  
Orange Fruit ◽  
Pcr Assays ◽  
Primer Sets

Two scab pathogens of citrus, Elsinoë fawcettii and E. australis, cause citrus scab and sweet orange scab, respectively, and pathotypes of each species have been described. The two species cannot be readily distinguished by morphological or cultural characteristics and can be distinguished only by host range and the sequence of the internal transcribed spacer (ITS) region. In this study, random amplified polymorphic DNA (RAPD) assays clearly distinguished E. fawcettii and E. australis, and the sweet orange and natsudaidai pathotypes within E. australis also could be differentiated. We developed specific primer sets, Efaw-1 for E. fawcettii; Eaut-1, Eaut-2, Eaut-3, and Eaut-4 for E. australis; and EaNat-1 and EaNat-2 for the natsudaidai pathotype within E. australis using RAPD products unique to each species or pathotype. Other primer sets, Efaw-2 and Eaut-5, which were specific for E. fawcettii and E. australis, respectively, were designed from previously determined ITS sequences. The Efaw-1 and Efaw-2 primer sets successfully identified E. fawcettii isolates from Korea, Australia, and the United States (Florida) and the Eaut-1 to Eaut-5 primer sets identified both the sweet orange pathotype isolates of E. australis from Argentina and the natsudaidai pathotype isolates from Korea. The EaNat-1 and EaNat-2 primer sets were specific for isolates of the natsudaidai pathotype. The Efaw-1 and Efaw-2 primer sets successfully detected E. fawcettii from lesions on diseased leaves and fruit from Korea and primer pairs Eaut-1, Eaut-2, Eaut-3, Eaut-4, and Eaut-5 detected E. australis from lesions on sweet orange fruit from Brazil.

scholarly journals Characterization of South American Snails of the GenusBiomphalaria(Basommatophora: Planorbidae) andSchistosoma mansoni(Platyhelminthes: Trematoda) in Molluscs by PCR-RFLP

2016 ◽  
Vol 2016 ◽  
pp. 1-5 ◽  
Author(s):  
Roberta Lima Caldeira ◽  
Tatiana Maria Teodoro ◽  
Liana Konovaloff Jannotti-Passos ◽  
Pollanah M. Lira-Moreira ◽  
Christiane De Oliveira Goveia ◽  
...  
Keyword(s):  
Latin American ◽  
Morphological Characteristics ◽  
Its Region ◽  
Morphological Identification ◽  
Correct Identification ◽  
Lack Of Information ◽  
Pcr Rflp ◽  
Mollusc Species ◽  
Identification Of Species ◽  
Molecular Profiles

The identification of snails of the genusBiomphalariacan be done using morphological characteristics which depends on the size of the snails and skill and knowledge of researcher. These methods sometimes are not adequate for identification of species. The PCR-RFLP, using the ITS region of the rDNA, has been used to identify Brazilian species of the genusBiomphalaria. Nevertheless, there is a lack of information about snails from other Latin American countries. In addition, some snails may be infected bySchistosoma mansoniand when submitted to PCR-RFLP they show molecular profiles different from those previously standardized for the other mollusc species. In this work the molecular profiles of 15 species and the subspecies were established by PCR-RFLP of ITS-rDNA with the enzymeDdeI. Moreover, the molecular profiles of host species,B. glabrata,B. straminea,B. tenagophila, andB. prona, infected byS. mansoniwere also established. The molluscs were dissected to permit morphological identification. These results contribute to a correct identification of snails of the genusBiomphalariaand detection of these snails infected byS. mansoni.

scholarly journals Molecular Profile of Trichophyton mentagrophytes and Microsporum canis based on PCR-RFLP of Internal Transcribed Spacer

2021 ◽  
Vol 26 (1) ◽  
pp. 10
Author(s):  
Dwi Endrawati ◽  
Eni Kusumaningtyas
Keyword(s):  
Internal Transcribed Spacer ◽  
Agar Plate ◽  
Its Region ◽  
Restriction Enzymes ◽  
Trichophyton Mentagrophytes ◽  
Molecular Profile ◽  
Specific Marker ◽  
Microsporum Canis ◽  
Fungal Identification ◽  
Pcr Rflp

<p><em>Trichophyton mentagrophytes</em> and <em>Microsporum canis</em> are dermatophytes fungi which commonly infect animal and human. Conventional and molecular methods were used for identification of the fungus. The region of internal transcribed spacer (ITS) has a high probability for fungal identification. PCR-RFLP was reported as a useful method to differentiate dermatophytes fungi. The objective of the study was<em> </em>to compare molecular profile of <em>T. mentagrophytes</em> and <em>M. canis</em> based on the result of ITS fragment digestion using Dde I, Hinf I and Mva I. The molds were isolated from skin scrapping of 18 animals which showed dermatophytosis lesion. The isolated molds were grown on agar plate for 14 days of incubation at 37<sup>o</sup>C and then identified based on macro and microscopic morphologies. Amplification of chitin synthase gene was used for confirmation and separation of dermatophytes from other fungi. ITS fragment was amplified and then digested using restriction enzymes Dde I, Hinf I and Mva I. The result showed that digestion products from ITS fragment of <em>T. mentagrophytes</em> and <em>M. canis </em>were different<em>.</em> The fragment 159 bp from Dde I, 374 bp from Hinf I and 89 bp from Mva I were present in <em>T. mentagrophytes</em> but absent in <em>M. canis</em>.   Based on these results, specific RFLP profile of digestion ITS region by Dde I, Hinf I and Mva I can be used as a specific marker for species of dermatophytes fungi.</p>

Sequence-based Identification and Classification of Fungi.

2021 ◽  
pp. 198-216
Author(s):  
Andrew M. Borman ◽  
Elizabeth M. Johnson
Keyword(s):  
Dna Sequences ◽  
Its Region ◽  
Pcr Primers ◽  
Taxonomic Resolution ◽  
Its2 Region ◽  
Rrna Gene ◽  
5.8S Rrna Gene ◽  
Fungal Identification ◽  
Identification Of Fungi ◽  
Fungal Dna

Abstract This book chapter describes the advantages and limitations of the ITS Region as a universal barcode for fungal identification. The ITS region offers several practical advantages as a universal fungal barcode region. The region encompasses segments that permit resolution at different taxonomic levels as it includes the highly conserved 5.8S rRNA gene, the moderately rapidly evolving ITS2 region and the rapidly evolving ITS1 region, flanked by the highly conserved SSU and LSU genes which permit design of PCR primers that are almost panfungal. Over the last two decades the sequence-based identification of fungi has certainly come of age. The ITS region is universally accepted as the primary fungal barcoding region owing to the high barcode gap with the locus for many groups of fungi. Since the species-resolution power of ITS is poor for certain groups of fungi, and higher-level taxonomic resolution is greater with proteincoding genes, the TEF1α locus has been proposed as the universal secondary barcode region. In addition, the historical problems surrounding the reliability of fungal DNA sequences in centralized repositories are slowly being resolved by the development of an increasing number of publicly accessible, curated databases.

scholarly journals Identifikasi Spesies Fungi Microsporum gypseum dan M. nanum Penyebab Ringworm pada Sapi Bali (IDENTIFICATION OF SPECIES FUNGI MICROSPORUM GYPSEUM

2018 ◽  
Vol 19 (2) ◽  
pp. 177
Author(s):  
Putu Ayu Sisyawati Putriningsih ◽  
I Putu Gede Yudhi Arjentinia
Keyword(s):  
Topical Therapy ◽  
Hair Shaft ◽  
Microsporum Gypseum ◽  
Direct Microscopic Examination ◽  
Superficial Skin ◽  
Hair Samples ◽  
Cotton Blue ◽  
Identification Of Species ◽  
Bali Cattle ◽  
Positive Results

Ringworm is an zoonotic infectious skin disease that can infect many types of animals. This disease is caused by dermatophytes fungi. Ringworm cases in cattle were quite widely reported in various countries but a report about the dermatophytes fungi that caused ringworm in bali cattle have never been published. It is very important in the efforts to give more effective therapies. This study aimed to identify the species of dermatophytes fungi that caused ringworm in bali cattle. Eight bali cattles suspected ringworm samples were taken using superficial skin scraping and trichogram (hair pluck) technique.  The samples of skin scrapings dan hair on the area of the lesion were taken for direct microscopic examination to find the presence of fungal elements (hyphae or arthrospora). They were dropped with 10% KOH, were allowed for 10-15 minutes, then were observed using microscope. Skin scrapings dan hair samples that showed positive results were cultured on Sabauroud’s Dextrose Agar (SDA) medium for 1-3 weeks dan were identified using Lactophenol Cotton Blue with microscope. The data were analyzed descriptively. Dermatophytes fungi that isolated dan identified were Microsporum gypseum (75% or 6/8) and Microsporum nanum (25% or 2/8). Both of these fungi have ectothrix invasion/infection tipe, which is the forming of arthrospores/arthroconidia is only on the surface/superficial hair shaft therefore the topical therapy is sufficient to resolve the infection. 

scholarly journals A review on the molecular characterization of digenean parasites using molecular markers with special reference to ITS region

2015 ◽  
Vol 52 (3) ◽  
pp. 167-187 ◽  
Author(s):  
K. Choudhary ◽  
A. Kumar Verma ◽  
S. Swaroop ◽  
N. Agrawal
Keyword(s):  
Molecular Markers ◽  
Its Region ◽  
Farm Animals ◽  
New Genera ◽  
Specific Level ◽  
Rdna Sequences ◽  
Region Data ◽  
Identification Of Species ◽  
Immense Potential

Abstract The rDNA region of eukaryotes has the immense potential to resolve the evolutionary and phylogeny problems using molecular markers. As evident from the present review, ITS region data is considered for interpretation of inter and intra-specific variations of 136 studies of 33 families including 78 genus and 114 species affecting individuals worldwide. Along with ITS-1 and ITS-2 region in 29 studies 18S region, in 38 studies 28S region and in 43 studies mitochondrial genes (COI and NDI) were also analyzed. Three new genera (Allobilharzia gen. nov., Caulanus gen. nov., and Latuterus gen. nov.) and 49 new species were discovered. Only 47 studies expressed variations at intra-specific and inter-specific level in complete ITS region, ITS-1 and ITS-2 rDNA sequences due to differences in nucleotide positions. According to the findings ITS region is more reliable and precise marker for demarcation and identification of species in combination of other DNA markers. Major studies were involved around the parasites of families Fasciolidae, Schistosomatidae, Opisthorchidae, Paragonimidae and Paramphistomidae, Clinostomidae, Diplostomidae, Haploporidae, among others infecting humans, farm animals, birds, fishes, reptiles and amphibians on the clinical basis. In future, molecular and bioinformatics aspects based on genetic variations will lead to explore the untouched areas of trematodes.

scholarly journals Internal Transcribed Spacer (ITS) as Dna Barcoding to Identify Fungal Species: a Review

2016 ◽  
Vol 11 (2) ◽  
pp. 37 ◽  
Author(s):  
Nurrahmi Dewi Fajarningsih
Keyword(s):  
Dna Barcoding ◽  
Internal Transcribed Spacer ◽  
Nuclear Dna ◽  
Dna Barcode ◽  
Its Region ◽  
Fungal Species ◽  
Species Level ◽  
Coding Region ◽  
Living Things ◽  
Fungal Identification

Despite the fact that fungi are important sources of both bioactive compounds and mycotoxins, and that they are very ubiquitous in our environment, their species identification is hampered by incomplete and often unclear literature. Fungi identification is primarily based on their phenotypic and physiological characteristics. Nowadays, many molecular methods to identify fungal species have been developed. One of the methods considered as a new concept to rapidly and accurately identify unknown fungal sample is DNA Barcoding. This literature review will outline the use of DNA barcoding approach to rapidly identify fungal species and the use of ITS region that recently has been designated as primary DNA barcode for fungal kingdom. “DNA barcode” is a short, highly variable and standardized DNA region with approximately 700 nucleotides in length, which is used as a unique pattern to identify living things. Internal Transcribed Spacer (ITS) region of nuclear DNA (rDNA) has become the most sequenced region to identify fungal taxonomy at species level, and even within species. ITS region is a highly polymorphic non-coding region with enough taxonomic units. Therefore, it is able to separate sequences into species level. Even though ribosomal ITS as a universal barcode marker for fungi is still hampered by few limitations, the ITS will remain as the key choice for fungal identification. The search for alternative regions as DNA marker to improve fungal identification, especially in specific heredities, has already started. 

scholarly journals Lipomyces chichibuensis sp. nov., isolated in Japan, and reidentification of the type strains of Lipomyces kononenkoae and Lipomyces spencermartinsiae

2014 ◽  
Vol 64 (Pt_8) ◽  
pp. 2566-2572 ◽  
Author(s):  
Atsushi Yamazaki ◽  
Hiroko Kawasaki
Keyword(s):  
Large Subunit ◽  
Elongation Factor ◽  
Its Region ◽  
Rrna Gene ◽  
Culture Collections ◽  
Phenotypic Characters ◽  
Lipomyces Kononenkoae ◽  
D2 Domain ◽  
Identification Of Species ◽  
Type Strains

We isolated two strains of a novel Lipomyces species from soil collected in Chichibu forest, Saitama prefecture, Japan. Based on their morphological and biochemical characteristics, along with multilocus sequence typing using the D1/D2 domain of the large-subunit (LSU) rRNA gene, the internal transcribed spacer (ITS) region and the translation elongation factor 1 alpha gene (EF-1α), the two strains were shown to represent a novel species of the genus Lipomyces, described as Lipomyces chichibuensis sp. nov. (type strain CB08-2T = NBRC 109582T = CBS 12929T; Mycobank no. MB808164). In addition, we reidentified the type strains of Lipomyces kononenkoae and Lipomyces spencermartinsiae maintained in culture collections based on phenotypic characters and/or DNA–DNA hybridization to ensure correct future identification of species of the genus Lipomyces. The correct type strains of L. kononenkoae and L. spencermartinsiae are NBRC 107661T ( = CBS 2514T) and NBRC 10376T ( = CBS 5608T), respectively.

scholarly journals DNA-based molecular identification of Urnula mediterranea (Ascomycota, Pezizales) collected in Central Serbia

2021 ◽  
pp. 53-62
Author(s):  
Dejan Arsenijević ◽  
Stefan Blagojević ◽  
Nevena Planojević ◽  
Aleksandra Nikezić ◽  
Dejan Vidanović ◽  
...  
Keyword(s):  
Genetic Markers ◽  
Molecular Systematics ◽  
Molecular Identification ◽  
Dna Sequences ◽  
Morphological Characteristics ◽  
Its Region ◽  
28S Rrna ◽  
Dna Molecules ◽  
Identification Of Species ◽  
First Time

Molecular systematics is a branch of systematics that analyzes genetic markers for the classification of organisms. Genetic markers include target sequences of molecules DNA, RNA, and amino acids. The most often used genetic markers are DNA markers, localized on DNA molecules. The study aim was DNA-based molecular identification of species Urnula mediterranea (M. Carbone, Agnello & Baglivo) M. Carbone, Agnello & P. Alvarado (2013), by using molecular systematics methods. This species was recently discovered for the first time in Central Serbia and determined only based on morphological characteristics. For the first time, U. mediterranea sampled in Serbia, was identified with DNA molecular markers. Extraction of DNA molecules was performed from the fruiting body of U. mediterranea. ITS region and the part of the gene for 28S rRNA were amplified and sequenced by the Sanger method. Comparing the analyzed sequences with the sequences from the database, a match of 99.72% was found for the ITS region and 100% for the part of the gene for 28S rRNA. By molecular identification, it has been established that the sample belongs to the species U. mediterranea. Both analyzed DNA sequences of U. mediterranea were deposited into the NCBI database.

Localization of cell-surface and basement-membrane heparan-sulfate proteoglycans using the malaria circumsporozoite protein

1994 ◽  
Vol 52 ◽  
pp. 294-295
Author(s):  
U. Frevert ◽  
S. Sinnis ◽  
C. Cerami ◽  
V. Nussenzweig
Keyword(s):  
Heparan Sulfate ◽  
Protein A ◽  
Kidney Tissue ◽  
Plasmodium Species ◽  
Its Region ◽  
Basement Membranes ◽  
Heparan Sulfate Proteoglycans ◽  
Infected Mosquito ◽  
Complement Components ◽  
Region Ii

Malaria sporozoites, which invade hepatocytes within minutes after transmission by an infected mosquito, are covered with the circumsporozoite (CS) protein, which in all Plasmodium species contains the conserved region II-plus. This region is also found as a cell-adhesive motif in a variety of host proteins like thrombospondin, properdin and the terminal complement components.The CS protein with its region II-plus specifically binds to heparan sulfate proteoglycans (HSPG) on the basolateral surface of hepatocytes in the space of Disse (FIG. 1), to certain basolateral cell membranes and basement membranes of the kidney (FIG. 2) as well as to heparin in the granules of connective tissue mast cells. The distribution of the HSPG receptors for the CS protein was examined by incubation of Lowicryl K4M or LR White sections of liver and kidney tissue with the recombinant CS ligand, whose binding sites were detected with a monoclonal anti-CS antibody and protein A gold.

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