Study, Nonylphenol Analysis of Textile Liquid Discharges of the City of Marrakech

Nonyphenols, especially the ethoxylated derivatives of nonylphenol (NPE) known as ethoxylated alkylphenols (APEs), are synthesized for their surfactant properties, which allow a better dispersion of liquids and the miscibility of certain substances such as oil and water. More than 40 years have been used as detergents, emulsifiers, wetting agents and dispersing agents and even cosmetic products such as shampoos. These organic molecules are very persistent and well known by their predatory toxicities on the skin, the eye, the respiratory tract, the kidneys and the liver, they are endocrine disruptors. The textile industry via some ennobling chemicals, tissue contamination and liquid discharges by these molecules remains a concern for the industry and the quality of these discharges. Our goal is to verify the contamination of liquid discharges from a textile industry using certain chemicals in the dyeing process.

Amputation

The decision to amputate rather than reconstruct a severely injured limb (‘mangled extremity’) has historically been one of the most difficult choices faced by a trauma surgeon. The surgeon’s responsibility is heightened by the knowledge that delayed or incorrect decision-making may lead to worse outcomes. Unfortunately, hard data upon which to base reliable decisions remain elusive. A prospective analysis of the use of scoring systems including the Limb Salvage Index, the Predictive Salvage Index, the Hanover Fracture Scale, and the NISSSA (Nerve injury, Ischaemia, Soft-tissue contamination, Skeletal damage, Shock, Age) and MESS (Mangled Extremity Severity Score) scores did not validate the clinical utility of any of the scoring algorithms.

BACTERIOLOGICAL CHARACTERISTICS OF THE EFFECTIVENESS OF VARIOUS METHODS OF ACCIDENTAL WOUND DRAINAGE

The article presents data on the bacteriological control of the effectiveness of various methods of draining fresh accidental wounds with a wide area of damage in different species of animals: semipermeable membranes and tubular perforated drains. Semi-permeable cellulose membranes (experimental group) were inserted into the wound of animals with absolute indications for drainage at the final stage of surgical treatment.These membranes were filled with a hyperosmolar dialysis solution containing antibacterial preparations. The comparison group used the introduction of antiseptics through perforated tubular drains. In order to study the quantitative and qualitative composition of microflora in the wound process, bacteriological studies of swabbings from the wound surface and from the wound canal were performed on 1-3-7-9 days. As the result, it was established that the degree of tissue contamination depends on the duration of surgical debridement ,type and conditions of animals. The study of quantitative composition of micro flora in 1 g of tissues, in farm animals, with accidental wounds was up to 105 . The appliance of wound dialysis gave rapid destruction of associations of 2 and 3 types of microorganisms, in contrast to the control group of animals. The effect of applying membrane dialysis devices, in case of accidental wounds, allows to achieve positive results for 3-4 days. Bacteriological studies confirm the high antibacterial activity of dialysate, which was provided by the introduction of antibiotics and antiseptics into its composition. The introduction of antibiotics and antiseptics into the membrane drainage cavity once a day in the amount of a single therapeutic dose, allows for 7.1±1.3 days (P>0.001) to suppress the microflora in the wound. Higher antiseptic effectiveness of dialysates from semipermeable membranes is connected with their ability to create a constant high concentration of antibacterial substances in the wound focus. The mechanism of therapeutic effect is in a gradual and constant flow of antiseptics into the paravulnar tissues, by diffusion. This improves the conditions for the development of the body’s own phylactic power against infection and raises regenerative processes in the wound.

1191. Hand Hygiene Education: Why Is It so Critical to Tissue Banking?

Background In the United States, the most commonly transplanted tissues are bones, tendons, skin, heart valves, vessels and corneas. Over two million tissue grafts are distributed each year.Efforts to advance tissue transplant safety are less defined compared with organ transplant, and continuously being improved and regulated by national agencies. Environmental contamination, postmortem translocation of microorganisms, cross-contamination, and tissue processing are critical components which can be prevented with hand hygiene education thus preventing the transmission of infectious diseases. Microorganisms from technicians’ skin can potentially result in infection and nontransplantable tissue. This is particularly true of regenerative tissue, such as Vivigen, that undergo no sterilization process. Methods A review was done of all 2018 ViviGen culture with these organisms: Bacillus, Cornebacterium, Diptheroids, Propionibacterium, Staphylococcus Coagulase negative, and Streptococcus viridians defined as Category 1. Results In 2018, 384 out of 1418 ViviGen donors were discarded due to positive cultures. Of the 384, 190 were due the presence of a Cat. 1 microorganism in post-processing cultures, for a discard rate of 17.6%. In these donors, preprocessing cultures were initially negative, thus indicating contamination was due to technician contamination. In the first quarter of 2019, and since implementation of Hand Hygiene education, we have already start to see a decline in acquisition of Cat 1 organism for a total reduction of 6% from Q1 of 2018 compared with 2019. Conclusion Tissue banking is a complex process of many steps to assure quality and safety of the tissue. Contamination is critical even when the microorganisms are considered low-virulent skin flora (i.e., Cat 1). This is especially important if these Cat 1 organisms are found in Post-processing cultures of grafts not terminally irradiated or sterilized. Implementation of proper hand hygiene education and aseptic techniques among tissue recovery and processing staff can significantly reduce the bioburden. Furthermore, a reduction in bioburden equates to higher likelihood in honoring the gift of donation in this selfless act of the donor and their loved one while also decreasing the financial loss due to culture results. Disclosures All authors: No reported disclosures.

Genetic variants and copy number changes in soft tissue leiomyosarcoma detected by targeted amplicon sequencing

AimsLeiomyosarcomas (LMSs) occur in various tissues and harbour potential for metastases. The genomic landscape of LMS is poorly understood. In an effort to improve understanding of the LMS genome, we analysed 11 LMSs of somatic soft tissue including matching tissue of normal phenotype.MethodsDNA derived from microdissected tumour domains and matching normal tissue underwent amplicon sequencing of 409 tumour suppressors and oncogenes using the Ion Torrent Comprehensive Cancer Panel.ResultsGenomic changes were heterogeneous with few recurrent abnormalities detected. Coding variants were identified in genes involved in signal transduction, transcriptional regulation and DNA repair. There were variants in several genes related to angiogenesis and GPR124 variants (TEM5) were confirmed by immunohistochemical analysis of a LMS tissue microarray. Surprisingly, there were shared coding variants in tumour and corresponding normal tissue.ConclusionsLMSs are a very heterogeneous population lacking recurrent somatic abnormalities. The presence of damaging mutations in normal tissue may reflect either a germline predisposition or field effect rather than tissue contamination. Hopeful therapeutic targets appear to be those related to AKT/MTOR pathway.

Virtual Screening for the Development of New Effective Compounds Against Staphylococcus aureus

Staphylococcus aureus is a notorious pathogenic bacterium causing a wide range of diseases from soft-tissue contamination, to more serious and deep-seated infections. This species is highlighted by its ability to express several kinds of virulence factors and to acquire genes related to drug resistance. Target this number of factors to design any drug is not an easy task. In this review, we discuss the importance of computational methods to impulse the development of new drugs against S. aureus. The application of docking methods to screen large libraries of natural or synthetic compounds and to provide insights into action mechanisms is demonstrated. Particularly, the studies that validated in silico results with biochemical and microbiological assays are highlighted. We also comment on the computer-aided design of new molecules using some known inhibitors. The confirmation of in silico results with biochemical and microbiological assays allowed the identification of lead molecules that could be used for drug design such as rhodomyrtone, quinuclidine, berberine (and their derivative compounds). The fast development of the computational methods is essential to improve our ability to discover new drugs, as well as to expand understanding about drug-target interactions.