scholarly journals Arabidopsis MADS-box transcription factor AGL21 acts as environmental surveillance for seed germination by regulating ABI5

2016 ◽  
Author(s):  
Lin-Hui Yu ◽  
Jie Wu ◽  
Zi-Qing Miao ◽  
Ping-Xia Zhao ◽  
Zhen Wang ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Seed Germination ◽  
Chromatin Immunoprecipitation ◽  
Plant Cells ◽  
Vital Role ◽  
Environmental Cues ◽  
Mads Box ◽  
Aba Signaling ◽  
Environmental Surveillance ◽  
Aba Insensitive

ABSTRACTSeed germination is a crucial checkpoint for plant survival under unfavorable environmental conditions. Abscisic acid (ABA) and its signaling play a vital role in integrating environmental information to regulate seed germination. MCM1/AGAMOUS/DEFICIENS/SRF (MADS)-box transcription factors are mainly known as key regulators of seed and flower development in Arabidopsis. However, their functions in seed germination are still poorly understood. Here we report that MADS-box transcription factor AGL21 negatively modulates seed germination and post-germination growth by controlling the expression of ABA-INSENSITIVE 5 (ABI5) in Arabidopsis. AGL21 responds to multiple environmental stresses and plant hormones. The AGL21-overexpressing plants are hypersensitive to ABA, salt and osmotic stresses during seed germination and early post-germination growth, whereas agl21 mutants are less sensitive. AGL21 positively regulates ABI5 expression in seeds. Genetic analyses reveal that AGL21 is epistatic to ABI5 in controlling seed germination. Chromatin immunoprecipitation assays further demonstrate that AGL21 could directly bind to the ABI5 promoter in plant cells. Taken together, our results suggest that AGL21 acts as a surveillance integrator that incorporates environmental cues and endogenous hormonal signals into ABA signaling to regulate seed germination and early post-germination growth.

scholarly journals Arabidopsis LSH8 Positively Regulates ABA Signaling by Changing the Expression Pattern of ABA-Responsive Proteins

2021 ◽  
Vol 22 (19) ◽  
pp. 10314
Author(s):  
Jinpeng Zou ◽  
Zhifang Li ◽  
Haohao Tang ◽  
Li Zhang ◽  
Jingdu Li ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Seed Germination ◽  
Signaling Pathway ◽  
Expression Pattern ◽  
Large Scale ◽  
Aba Signaling ◽  
Wild Type ◽  
Floral Organogenesis ◽  
Early Seedling ◽  
Aba Insensitive

Phytohormone ABA regulates the expression of numerous genes to significantly affect seed dormancy, seed germination and early seedling responses to biotic and abiotic stresses. However, the function of many ABA-responsive genes remains largely unknown. In order to improve the ABA-related signaling network, we conducted a large-scale ABA phenotype screening. LSH, an important transcription factor family, extensively participates in seedling development and floral organogenesis in plants, but whether its family genes are involved in the ABA signaling pathway has not been reported. Here we describe a new function of the transcription factor LSH8 in an ABA signaling pathway. In this study, we found that LSH8 was localized in the nucleus, and the expression level of LSH8 was significantly induced by exogenous ABA at the transcription level and protein level. Meanwhile, seed germination and root length measurements revealed that lsh8 mutant lines were ABA insensitive, whereas LSH8 overexpression lines showed an ABA-hypersensitive phenotype. With further TMT labeling quantitative proteomic analysis, we found that under ABA treatment, ABA-responsive proteins (ARPs) in the lsh8 mutant presented different changing patterns with those in wild-type Col4. Additionally, the number of ARPs contained in the lsh8 mutant was 397, six times the number in wild-type Col4. In addition, qPCR analysis found that under ABA treatment, LSH8 positively mediated the expression of downstream ABA-related genes of ABI3, ABI5, RD29B and RAB18. These results indicate that in Arabidopsis, LSH8 is a novel ABA regulator that could specifically change the expression pattern of APRs to positively mediate ABA responses.

scholarly journals Arabidopsis MADS-Box Transcription Factor AGL21 Acts as Environmental Surveillance of Seed Germination by Regulating ABI5 Expression

2017 ◽  
Vol 10 (6) ◽  
pp. 834-845 ◽  
Author(s):  
Lin-Hui Yu ◽  
Jie Wu ◽  
Zi-Sheng Zhang ◽  
Zi-Qing Miao ◽  
Ping-Xia Zhao ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Seed Germination ◽  
Mads Box ◽  
Environmental Surveillance

scholarly journals ARABIDOPSIS NITRATE REGULATED 1 acts as a negative modulator of seed germination by activating ABI3 expression

2019 ◽  
Author(s):  
Jia-Hui Lin ◽  
Lin-Hui Yu ◽  
Cheng-Bin Xiang
Keyword(s):  
Seed Germination ◽  
Chromatin Immunoprecipitation ◽  
Mads Box ◽  
Knockout Mutant ◽  
Plant Life ◽  
Similar Expression Pattern ◽  
Early Seedling ◽  
Safeguard Mechanism ◽  
Early Seedling Development ◽  
Salt And Osmotic Stress

ABSTRACTSeed germination is a crucial transition point in plant life and is tightly regulated by environmental conditions through the coordination of two phytohormones, gibberellin and abscisic acid (ABA). To avoid unfavorable conditions, plants have evolved safeguard mechanisms for seed germination. Here, we report a novel function of the Arabidopsis MADS-box transcription factor ARABIDOPSIS NITRATE REGULATED 1 (ANR1) in seed germination. ANR1 knockout mutant is insensitive to ABA, salt, and osmotic stress during the seed germination and early seedling development stages, whereas ANR1-overexpressing lines are hypersensitive. ANR1 is responsive to ABA and abiotic stresses and upregulates the expression of ABI3 to suppress seed germination. ANR1 and ABI3 have similar expression pattern during seed germination. Genetically, ABI3 acts downstream of ANR1. Chromatin immunoprecipitation and yeast-one-hybrid assays showed that ANR1 could bind to the ABI3 promoter to regulate its expression. In addition, ANR1 acts synergistically with AGL21 to suppress seed germination in response to ABA as evidenced by anr1 agl21 double mutant. Taken together, our results demonstrate that the ANR1 plays an important role in regulating seed germination and early post-germination growth. ANR1 and AGL21 together constitutes a safeguard mechanism for seed germination to avoid unfavorable conditions.

scholarly journals O-fucosylation of CPN20 by SPINDLY Derepresses Abscisic Acid Signaling During Seed Germination and Seedling Development

2021 ◽  
Vol 12 ◽  
Author(s):  
Lin Liang ◽  
Qi Wang ◽  
Zihao Song ◽  
Yaxin Wu ◽  
Qing Liang ◽  
...  
Keyword(s):  
Seed Germination ◽  
Mammalian Cells ◽  
Electron Transfer Dissociation ◽  
Seedling Development ◽  
Aba Signaling ◽  
Early Seedling ◽  
Aba Insensitive ◽  
Early Seedling Development

SPINDLY is involved in some aspects of plant development. However, the nature of this protein as an O-fucosyltransferase was recently discovered. In this study, we show that SPINDLY (SPY) interacts with CPN20 in yeast two-hybrid and split-luc assays, and the interaction is promoted by ABA. CPN20 is a chloroplast-localized co-chaperonin that negatively regulates ABAR-mediated ABA signaling. By using Electron Transfer Dissociation-MS/MS analysis, two O-fucosylation sites, e.g., 116th and 119th threonines, were detected in ectopically expressed CPN20 in mammalian cells and in Arabidopsis. The O-fucosylation at both threonine residues was confirmed by in vitro peptide O-fucosylation assay. We further show that CPN20 accumulates in the chloroplast of spy mutants, suggesting that SPY negatively regulates CPN20 localization in the chloroplast. In vivo protein degradation assay along with CPN20 localization behavior suggest that import of CPN20 into the chloroplast is negatively regulated by SPY. Genetic analysis shows that ABA insensitive phenotypes of spy-3 in terms of seed germination and early seedling development are partially suppressed by the cpn20 mutation, suggesting that CPN20 acts downstream of SPY in this ABA signaling pathway and that there may exist other pathways in parallel with CPN20. Collectively, the above data support the notion that the O-fucosylation of CPN20 by SPY fine-tunes ABA signaling in Arabidopsis.

scholarly journals MADS-Box Transcription Factor Mig1 Is Required for Infectious Growth in Magnaporthe grisea

2008 ◽  
Vol 7 (5) ◽  
pp. 791-799 ◽  
Author(s):  
Rahim Mehrabi ◽  
Shengli Ding ◽  
Jin-Rong Xu
Keyword(s):  
Transcription Factor ◽  
Map Kinases ◽  
Magnaporthe Grisea ◽  
Fluorescent Protein ◽  
Plant Cells ◽  
Normal Growth ◽  
Defense Responses ◽  
Mads Box ◽  
Plant Interactions ◽  
Plant Infection

ABSTRACT Magnaporthe grisea is a model fungus for studying fungus-plant interactions. Two mitogen-activated protein (MAP) kinase genes, PMK1 and MPS1, have been implicated in regulating plant infection processes in M. grisea. However, transcription factors activated by these MAP kinases are not well studied. In this study we functionally characterized the MIG1 gene that encodes a MADS-box transcription factor homologous to Saccharomyces cerevisiae Rlm1. In yeast two-hybrid assays, MIG1 interacts with MPS1, suggesting that MIG1 may function downstream from the MPS1 pathway. The mig1 deletion mutant had a normal growth rate and formed melanized appressoria, but it was nonpathogenic and failed to infect rice leaves through wounds. Appressoria formed by the mig1 mutant developed penetration pegs and primary infectious hyphae, but further differentiation of the secondary infectious hyphae inside live plant cells was blocked. However, the mig1 mutant formed infectious hypha-like structures in heat-killed plant cells or cellophane membranes. In transformants expressing the MIG1-GFP fusion, green fluorescent protein (GFP) signals were not detectable in vegetative hyphae and conidiophores. Mig1-GFP was localized to nuclei in conidia, appressoria, and infectious hyphae. Deletion of the MADS box had no effect on the expression and localization of the MIG1-GFP fusion but eliminated its ability to complement the mig1 mutant. These results suggest that MIG1 may be required for overcoming plant defense responses and the differentiation of secondary infectious hyphae in live plant cells. The MADS-box domain is essential for the function of MIG1 but dispensable for its nuclear localization, which may be associated with the activation of MIG1 by MPS1 during conidiation and plant infection.

scholarly journals The Multifaceted Regulation of SnRK2 Kinases

Cells ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 2180
Author(s):  
Justyna Maszkowska ◽  
Katarzyna Patrycja Szymańska ◽  
Adrian Kasztelan ◽  
Ewa Krzywińska ◽  
Olga Sztatelman ◽  
...  
Keyword(s):  
Plant Cells ◽  
Molecular Switches ◽  
Environmental Cues ◽  
Plant Responses ◽  
Controversial Issues ◽  
Aba Signaling ◽  
Independent Manner ◽  
New Information ◽  
Favorable Conditions ◽  
Short Time

SNF1-related kinases 2 (SnRK2s) are central regulators of plant responses to environmental cues simultaneously playing a pivotal role in the plant development and growth in favorable conditions. They are activated in response to osmotic stress and some of them also to abscisic acid (ABA), the latter being key in ABA signaling. The SnRK2s can be viewed as molecular switches between growth and stress response; therefore, their activity is tightly regulated; needed only for a short time to trigger the response, it has to be induced transiently and otherwise kept at a very low level. This implies a strict and multifaceted control of SnRK2s in plant cells. Despite emerging new information concerning the regulation of SnRK2s, especially those involved in ABA signaling, a lot remains to be uncovered, the regulation of SnRK2s in an ABA-independent manner being particularly understudied. Here, we present an overview of available data, discuss some controversial issues, and provide our perspective on SnRK2 regulation.

scholarly journals Expression of the Candida albicans Morphogenesis Regulator Gene CZF1 and Its Regulation by Efg1p and Czf1p

2006 ◽  
Vol 5 (5) ◽  
pp. 825-835 ◽  
Author(s):  
Marcelo D. Vinces ◽  
Christopher Haas ◽  
Carol A. Kumamoto
Keyword(s):  
Transcription Factor ◽  
Candida Albicans ◽  
Chromatin Immunoprecipitation ◽  
Physical Environment ◽  
Growth Parameters ◽  
Environmental Cues ◽  
Environmental Signals ◽  
Putative Transcription Factor ◽  
Morphological Transitions ◽  
Important Regulator

ABSTRACT The ability of Candida albicans to transit between different cellular morphologies is believed to be important for virulence. Morphological transitions occur in response to a variety of environmental signals. One such signal is encountered when cells are grown in a semisolid matrix. An important regulator of cellular morphology is the putative transcription factor CZF1. Here we demonstrate that transcription of CZF1 is responsive to growth parameters such as the temperature, carbon source, growth phase of cells, and the physical environment. In wild-type cells, a CZF1 transcript of about 4 kb was expressed when cells were grown embedded in semisolid agar medium, as well as in late exponential phase when cells were grown in liquid medium. Deletion of EFG1, a key regulator of morphogenesis, abolished CZF1 expression. Overexpression of CZF1 revealed that this gene also autoregulates its expression. Efg1p and Czf1p were shown by chromatin immunoprecipitation to act by binding to the promoter of CZF1. The coupling of environmental cues to the expression of a morphogenetic transcription factor may allow C. albicans to coordinate morphogenesis in response to specific conditions encountered in the human host.

scholarly journals Low ABA concentration promotes root growth and hydrotropism through relief of ABA INSENSITIVE 1-mediated inhibition of plasma membrane H+-ATPase 2

2021 ◽  
Vol 7 (12) ◽  
pp. eabd4113
Author(s):  
Rui Miao ◽  
Wei Yuan ◽  
Yue Wang ◽  
Irene Garcia-Maquilon ◽  
Xiaolin Dang ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Root Growth ◽  
Experimental System ◽  
Aba Signaling ◽  
Wild Type ◽  
Normal Medium ◽  
C Terminus ◽  
Quadruple Mutant ◽  
Aba Insensitive ◽  
Aba Receptors

The hab1-1abi1-2abi2-2pp2ca-1 quadruple mutant (Qabi2-2) seedlings lacking key negative regulators of ABA signaling, namely, clade A protein phosphatases type 2C (PP2Cs), show more apoplastic H+ efflux in roots and display an enhanced root growth under normal medium or water stress medium compared to the wild type. The presence of low ABA concentration (0.1 micromolar), inhibiting PP2C activity via monomeric ABA receptors, enhances root apoplastic H+ efflux and growth of the wild type, resembling the Qabi2-2 phenotype in normal medium. Qabi2-2 seedlings also demonstrate increased hydrotropism compared to the wild type in obliquely-oriented hydrotropic experimental system, and asymmetric H+ efflux in root elongation zone is crucial for root hydrotropism. Moreover, we reveal that Arabidopsis ABA-insensitive 1, a key PP2C in ABA signaling, interacts directly with the C terminus of Arabidopsis plasma membrane H+-dependent adenosine triphosphatase 2 (AHA2) and dephosphorylates its penultimate threonine residue (Thr947), whose dephosphorylation negatively regulates AHA2.

Sign in / Sign up

Export Citation Format

Share Document