scholarly journals Estimating densities of larval Salmonflies (Pteronarcys californica) through multiple pass removal of post-emergent exuvia in Colorado rivers

2019 ◽  
Author(s):  
Brian D. Heinold ◽  
Dan A. Kowalski ◽  
R. Barry Nehring

AbstractTraditional methods of collecting and sorting benthic macroinvertebrate samples are useful for stream biomonitoring and ecological studies; however, these methods are time consuming, expensive, and require taxonomic expertise. Estimating larval densities through collection of post-emergent exuvia can be a practical and time efficient alternative. We evaluated the use of multiple pass depletion techniques of the post-emergent exuvia of Pteronarcys californica to estimate larval densities at ten sites in three Colorado rivers. Exuvia density was highly correlated with both final-instar larval density (R2 = 0.90) and total larval density (R2 = 0.88) and the multiple pass removal technique performed well. Exuvia surveys found P. californica at three low density sites where benthic sampling failed to detect it. At moderate and high density sites the exuvia surveys always produced lower density estimates than benthic surveys. Multiple pass depletion estimates of exuvia proved to be an accurate and efficient technique at estimating larval densities and provided an effective alternative for traditional benthic sampling when objectives are monitoring P. californica and detecting populations, especially at low density sites.

1989 ◽  
Vol 24 (3) ◽  
pp. 344-347
Author(s):  
G. David Buntin ◽  
David J. Isenhour

The accuracy, precision and efficiency of stem-count and sweep-net techniques were compared for sampling the pea aphid, Acyrthosiphon pisum (Harris), in alfalfa. Density estimates by both techniques were highly correlated (r = 0.87). Both techniques were similar in sample precision and efficiency, but stem counts provided more accurate density estimates than the sweep net technique. The stem count technique is an accurate and efficient alternative to the sweep net for sampling pea aphids in alfalfa.


1961 ◽  
Vol 2 (2) ◽  
pp. 249-271 ◽  
Author(s):  
B. Burnet

An account is given of the habitat and ecology of a wrackbed population of Coelopa frigida (Fab.). The spatial distribution of the third-instar larvae was found to be patchy. This is due to a preference of adult flies for oviposition sites in zones of high temperature within the wrackbed. The relationship between larval density and temperature was found to account only in part for the patchy distribution, a second factor being the formation of family aggregates within each temperature zone. The effect of this type of spatial distribution on the interpretation of genetic sampling methods for the population is discussed.Samples of third-instar larvae from the wrackbed population have been analysed for the presence of recessive embryonic lethals in three consecutive years from 1957 to 1959. From a total of 528 gametes tested, 44 recessive embryonic lethals were isolated. Cross tests for allelism showed that certain lethals were repeated within samples taken in the same year.The gross frequency of embryonic lethals is shown to be independent of seasonal changes in population density. Estimates of gene frequency are made for certain individual lethals and these are shown to be greater than expected on the basis of the census size of the population. Possible causes of this discrepancy are examined.


Author(s):  
A. N. Kolesnikov ◽  
A. V. Dubovaya ◽  
Yu. V. Udovitchenko

The article presents data of experimental and clinical studies devoted to the influence of vitamin D on the origin and progression of the cardiovascular diseases: arterial hypertension, atherosclerosis, coronary heart disease, cardiac rhythm disturbances. The results of the studies indicate that a pronounced deficiency of vitamin D is highly correlated with sudden cardiac death, cardiovascular catastrophes, and overall mortality. The decrease in vitamin D is followed by an increase in total cholesterol, low-density lipoproteins and triglycerides, an increase in the heart rate and the level of systolic blood pressure. The Meta-analyzes of randomized controlled trials demonstrated that the addition of vitamin D to treatment and rehabilitation measures of the patients with cardiovascular disease helps to reduce overall mortality.


1995 ◽  
Vol 41 (2) ◽  
pp. 232-240 ◽  
Author(s):  
J R McNamara ◽  
T G Cole ◽  
J H Contois ◽  
C A Ferguson ◽  
J M Ordovas ◽  
...  

Abstract Low-density lipoprotein (LDL) cholesterol can not be calculated from other lipid measurements when samples are obtained from nonfasting individuals or when triglycerides are > or = 4.0 g/L. We have evaluated a direct LDL cholesterol assay for analyzing 115 fresh serum samples obtained from fasting and nonfasting dyslipidemic patients with triglycerides < or = 35.85 g/L, who were receiving diet and (or) drug treatments. Results were highly correlated with those by ultracentrifugation (r = 0.97), with a mean/median bias of -2.9%/0.7% (-0.001/0.010 g/L) and an absolute bias of 9.5%/6.4% (0.119/0.090 g/L). The assay correctly classified LDL cholesterol concentrations < 1.30 g/L 81% of the time, 1.30-1.60 g/L 76% of the time, and > or = 1.60 g/L 94% of the time. Precision studies provided within- and between-run CVs in the range of 1.2-3.8% and 2.0-5.1%, respectively. Our data indicate that this assay is an accurate method for measuring LDLC directly from fresh serum obtained from fasting or nonfasting subjects with a wide range of triglyceride values.


<em>Abstract.</em>—We evaluated several approaches for measuring natural and anthropogenic habitat characteristics to predict benthic macroinvertebrate assemblages over a range of urban intensity at 85 stream sites in the Santa Clara Valley, California. Land cover was summarized as percentage urban land cover and impervious area within upstream buffers and the upstream subwatersheds. Field measurements characterized water chemistry, channel slope, sediment, and riparian canopy. In addition to applying the visual-based habitat assessment in U.S. Environmental Protection Agency’s rapid bioassessment protocol, we developed a simplified urban habitat assessment index based on turbidity, fine sediment deposition, riparian condition, and channel modification. Natural and anthropogenic habitat variables covaried along longitudinal stream gradients and were highly correlated with elevation. At the scale of the entire watershed, benthic macroinvertebrate measures were equally correlated with variables expressing natural gradients and urbanization effects. When natural gradients were reduced by partitioning sites into ecoregion subsection groupings, habitat variables most highly correlated with macroinvertebrate measures differed between upland and valley floor site groups. Among the valley floor sites, channel slope and physical modification of channel and riparian habitats appeared more important than upstream land cover or water quality in determining macroinvertebrate richness and ordination scores. Among upland sites, effects of upstream reservoir releases on habitat quality appeared important. Rapid habitat evaluation methods appeared to be an effective method for describing habitat features important to benthic macroinvertebrates when adapted for the region and the disturbance of interest.


Author(s):  
Tate Fegley ◽  
Lisa Growette Bostaph

Purpose The purpose of this paper is to investigate whether the nature of policing services allows for economies of scale to be realized. It is also a replication of Southwick (2005). Design/methodology/approach This study replicates the methodology used by Southwick (2005) to estimate police production and demand in order to determine whether there are economies of scale among police departments in a western state. Southwick’s (2005) method is unique in that it incorporates measures of market power to predict police efficiency. The present study is unique in that it involves data from a low-density, low-population western state. Findings Southwick’s results for New York State are markedly different from the results found for Idaho, thus questioning the external validity of Southwick’s model as applied to a relatively low-population state. The findings also indicate that, controlling for relevant variables, crime in Idaho is highly correlated with population, suggesting that police departments in low density/population states would not achieve efficiency gains through consolidation. Research limitations/implications The implications of this study include validating police performance measures and evaluating applicability of market power to police departments. Practical implications No evidence was found to support the contention that consolidation of police departments results in efficiency gains. Originality/value This is the first study of economies of scale in policing to use data from a low-density, low-population western state.


2018 ◽  
Author(s):  
Io Long Chan ◽  
Oliver J. Rando ◽  
Colin C. Conine

ABSTRACTBleaching gravid C. elegans followed by a short period of starvation of the L1 larvae is a routine method performed by worm researchers for generating synchronous populations for experiments. During the process of investigating dietary effects on gene regulation in L1 stage worms by single-worm RNA-Seq, we found that the density of resuspended L1 larvae affects expression of many mRNAs. Specifically, a number of genes related to metabolism and signalling are highly expressed in worms arrested at low density, but are repressed at higher arrest densities. We generated a GFP reporter strain based on one of the most density-dependent genes in our dataset – lips-15 – and confirmed that this reporter was expressed specifically in worms arrested at relatively low density. Finally, we show that conditioned media from high density L1 cultures was able to downregulate lips-15 even in L1 animals arrested at low density, and experiments using daf-22 mutant animals demonstrated that this effect is not mediated by the ascaroside family of signalling pheromones. Together, our data implicate a soluble signalling molecule in density sensing by L1 stage C. elegans, and provide guidance for design of experiments focused on early developmental gene regulation.


Metabolites ◽  
2012 ◽  
Vol 2 (3) ◽  
pp. 458-478 ◽  
Author(s):  
Maria-Louisa Izamis ◽  
Korkut Uygun ◽  
Nripen S. Sharma ◽  
Basak Uygun ◽  
Martin L. Yarmush ◽  
...  

1983 ◽  
Vol 115 (11) ◽  
pp. 1437-1443 ◽  
Author(s):  
S. B. Ramaswamy ◽  
R. T. Cardé ◽  
J. A. Witter

AbstractLarval densities and catch of adult male Choristoneura fumiferana (Clemens) in pheromone-baited covered funnel traps in the same year were highly correlated. Such traps avoid saturation problems associated with conventional sticky traps. Release of marked males resulted in recapture rates of up to 21% in covered funnel traps. The findings suggest that non-saturating pheromone-baited traps could be used to monitor population densities and trends of the spruce budworm.


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