scholarly journals Bright and tunable far-red chemigenetic indicators

2020 ◽  
Author(s):  
Claire Deo ◽  
Ahmed S. Abdelfattah ◽  
Hersh K. Bhargava ◽  
Adam J. Berro ◽  
Natalie Falco ◽  
...  
Keyword(s):  
Functional Imaging ◽  
Action Potentials ◽  
Chemical Properties ◽  
The Self ◽  
Red Emission ◽  
Fluorescent Indicators ◽  
Single Action ◽  
Voltage Sensors ◽  
Environmentally Sensitive ◽  
And Chemical Properties

AbstractFunctional imaging using fluorescent indicators has revolutionized biology but additional sensor scaffolds are needed to access properties such as bright, far-red emission. We introduce a new platform for ‘chemigenetic’ fluorescent indicators, utilizing the self-labeling HaloTag protein conjugated to environmentally sensitive synthetic fluorophores. This approach affords bright, far-red calcium and voltage sensors with highly tunable photophysical and chemical properties, which can reliably detect single action potentials in neurons.

scholarly journals Changes in physical and chemical properties of niosome membrane induced by cholesterol: a promising approach for niosome bilayer intervention

RSC Advances ◽  
2017 ◽  
Vol 7 (78) ◽  
pp. 49463-49472 ◽  
Author(s):  
Mohammad Hadi Nematollahi ◽  
Abbas Pardakhty ◽  
Masoud Torkzadeh-Mahanai ◽  
Mehrnaz Mehrabani ◽  
Gholamreza Asadikaram
Keyword(s):  
Self Assembly ◽  
Nonionic Surfactants ◽  
Chemical Properties ◽  
The Self ◽  
Physical And Chemical Properties ◽  
Physical And Chemical ◽  
And Chemical Properties

Recently, the self-assembly property of nonionic surfactants has been utilized to create vesicles as alternatives to liposomes.

scholarly journals Fluorescent and bioluminescent calcium indicators with tuneable colors and affinities

2021 ◽  
Author(s):  
Nicole Mertes ◽  
Marvin Busch ◽  
Magnus-Carsten Huppertz ◽  
Christina Nicole Hacker ◽  
Clara-Marie Guerth ◽  
...  
Keyword(s):  
Action Potentials ◽  
Hippocampal Neurons ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Ease Of Use ◽  
Fluorescent Indicators ◽  
Biological Assays ◽  
Single Action ◽  
Calcium Indicators ◽  
Cellular Compartments

We introduce a family of bright, rhodamine-based calcium indicators with tuneable affinities and colors. The indicators can be specifically localized to different cellular compartments and are compatible with both fluorescence and bioluminescence readouts through conjugation to HaloTag fusion proteins. Importantly, their increase in fluorescence upon localization enables no-wash live-cell imaging, which greatly facilitates their use in biological assays. Applications as fluorescent indicators in rat hippocampal neurons include the detection of single action potentials and of calcium fluxes in the endoplasmic reticulum (ER). Applications as bioluminescent indicators include the recording of the pharmacological modulation of nuclear calcium in high-throughput-compatible assays. The versatility and remarkable ease of use of these indicators make them powerful tools for bioimaging and bioassays.

scholarly journals Effects of Structural Symmetry on NMR Spectra of 3,9-Diazatetraasteranes

2021 ◽  
Author(s):  
Runzhi Sun ◽  
Xiuqing Song ◽  
Fan Xie ◽  
Huiqin Wang ◽  
Hong Yan
Keyword(s):  
Nmr Spectra ◽  
Chemical Properties ◽  
2D Nmr ◽  
The Self ◽  
Physical And Chemical Properties ◽  
X Ray Diffraction ◽  
Structural Symmetry ◽  
X Ray ◽  
Physical And Chemical ◽  
And Chemical Properties

Abstract The structural symmetry is of great significance on the unique physical and chemical properties that closely related to pharmacological applications. To investigate the effects of structural symmetry on the NMR spectra, C2-3,9-diazatetraasteranes and non-C2-3,9-diazatetraasteranes were selected as the research object. They were synthesized by the self-dimerization and cross-dimerization of diethyl 1,4-dihydropyridine-3,5-dicarboxylates, respectively. The differences and similarities in NMR spectra of these two types of 3,9-diazatetraasteranes were discussed by the 1D-NMR and 2D-NMR analysis. The single-crystal X-ray diffraction (XRD) was used to demonstrate directly their structural symmetry.

scholarly journals Bioinspired supramolecular nanosheets of zinc chlorophyll assemblies

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Sunao Shoji ◽  
Tetsuya Ogawa ◽  
Shogo Matsubara ◽  
Hitoshi Tamiaki
Keyword(s):  
Self Assembly ◽  
Chemical Properties ◽  
The Self ◽  
Nano Materials ◽  
Flat Surfaces ◽  
Model Molecule ◽  
Chlorophyll Derivative ◽  
Physical And Chemical ◽  
Antenna Systems ◽  
And Chemical Properties

Abstract Two-dimensional sheet-like supramolecules have attracted much attention from the viewpoints of their potential application as functional (nano)materials due to unique physical and chemical properties. One of the supramolecular sheet-like nanostructures in nature is visible in the self-assemblies of bacteriochlorophyll-c–f pigments inside chlorosomes, which are major components in the antenna systems of photosynthetic green bacteria. Herein, we report artificial chlorosomal supramolecular nanosheets prepared by the self-assembly of a synthetic zinc 31-methoxy-chlorophyll derivative having amide and urea groups in the substituent at the 17-position. The semi-synthetic zinc chlorophyll derivative kinetically formed dimeric species and transformed into more thermodynamically stable chlorosomal J-aggregates in the solid state. The kinetically and thermodynamically formed self-assemblies had particle-like and sheet-like supramolecular nanostructures, respectively. The resulting nanosheets of biomimetic chlorosomal J-aggregates had flat surfaces and well-ordered supramolecular structures. The artificial sheet-like nanomaterial mimicking chlorosomal bacteriochlorophyll-c–f J-aggregates was first constructed by the model molecule, and is potentially useful for various applications including artificial light-harvesting antennas and photosyntheses.

Enhanced photothermal behavior derived from controllable self-assembly of Cu1.94S microstructures

2019 ◽  
Vol 48 (14) ◽  
pp. 4495-4503
Author(s):  
Xiao Shao ◽  
Tianyong Zhang ◽  
Bin Li ◽  
Minghao Zhou ◽  
Xiaoyuan Ma ◽  
...  
Keyword(s):  
Self Assembly ◽  
Chemical Properties ◽  
The Self ◽  
Physical And Chemical Properties ◽  
Assembly Process ◽  
Physical And Chemical ◽  
And Chemical Properties

New ordered architectures or morphologies could be obtained through the self-assembly process and usually generate new physical and chemical properties.

Tribological Properties and Wear Mechanism of Self-Lubricating Ni-P/PTFE

2012 ◽  
Vol 229-231 ◽  
pp. 134-137
Author(s):  
Deng Xu ◽  
Ning Li ◽  
Ji Jie Ma ◽  
Guang Ming Cheng ◽  
Lin Lin Wang
Keyword(s):  
Tribological Properties ◽  
Chemical Properties ◽  
Hexagonal Structure ◽  
The Self ◽  
Material Structure ◽  
X Ray Diffraction ◽  
Low Friction ◽  
X Ray ◽  
Chemical Plating ◽  
And Chemical Properties

The self-lubrication Ni-P/PTFE co-coatings were obtained by chemical plating. The tribological properties of the co-coating were carried on by the MM-200 type abrasion, and the microstructure and compounds of this composition and surface morphology were described by using X-ray diffraction and scanning electron microscope separately. The Ni-P/PTFE co-coating still had a low friction coefficient and good wear resistance. This was due to the PTFE material structure with the twisted chain, helical characteristics of carbon nanotubes, and the hexagonal structure in connection with many unusual mechanical and chemical properties.

Statistical criteria of stellar population types

1966 ◽  
Vol 24 ◽  
pp. 101-110
Author(s):  
W. Iwanowska
Keyword(s):  
Stellar Population ◽  
Chemical Properties ◽  
Spectrophotometric Study ◽  
Physical And Chemical Properties ◽  
Population Type ◽  
The Galaxy ◽  
Distribution Parameters ◽  
Physical And Chemical ◽  
Statistical Criteria ◽  
And Chemical Properties

In connection with the spectrophotometric study of population-type characteristics of various kinds of stars, a statistical analysis of kinematical and distribution parameters of the same stars is performed at the Toruń Observatory. This has a twofold purpose: first, to provide a practical guide in selecting stars for observing programmes, second, to contribute to the understanding of relations existing between the physical and chemical properties of stars and their kinematics and distribution in the Galaxy.

The microstructure of a TiB2/Ti-47 Al composite material

1989 ◽  
Vol 47 ◽  
pp. 674-675
Author(s):  
O. Popoola ◽  
A.H. Heuer ◽  
P. Pirouz
Keyword(s):  
Composite Material ◽  
Ion Beam ◽  
Chemical Properties ◽  
Intermetallic Alloys ◽  
Transmission Electron ◽  
Diamond Polishing ◽  
Al Composite ◽  
The Matrix ◽  
Argon Ion ◽  
And Chemical Properties

The addition of fibres or particles (TiB2, SiC etc.) into TiAl intermetallic alloys could increase their toughness without compromising their good high temperature mechanical and chemical properties. This paper briefly discribes the microstructure developed by a TiAl/TiB2 composite material fabricated with the XD™ process and forged at 960°C.The specimens for transmission electron microscopy (TEM) were prepared in the usual way (i.e. diamond polishing and argon ion beam thinning) and examined on a JEOL 4000EX for microstucture and on a Philips 400T equipped with a SiLi detector for microanalyses.The matrix was predominantly γ (TiAl with L10 structure) and α2(TisAl with DO 19 structure) phases with various morphologies shown in figure 1.

Tailoring microstructures of materials through biomimetics

1993 ◽  
Vol 51 ◽  
pp. 500-501
Author(s):  
Mehmet Sarikaya ◽  
Ilhan A. Aksay
Keyword(s):  
Chemical Properties ◽  
Design And Synthesis ◽  
Structure Sensitive ◽  
Macro Scale ◽  
Methods Of Synthesis ◽  
Successive Level ◽  
Engineering Materials ◽  
Physical And Chemical ◽  
And Chemical Properties ◽  
Synthesis Of Materials

Biomimetics involves investigation of structure, function, and methods of synthesis of biological composite materials. The goal is to apply this information to the design and synthesis of materials for engineering applications.Properties of engineering materials are structure sensitive through the whole spectrum of dimensions from nanometer to macro scale. The goal in designing and processing of technological materials, therefore, is to control microstructural evolution at each of these dimensions so as to achieve predictable physical and chemical properties. Control at each successive level of dimension, however, is a major challenge as is the retention of integrity between successive levels. Engineering materials are rarely fabricated to achieve more than a few of the desired properties and the synthesis techniques usually involve high temperature or low pressure conditions that are energy inefficient and environmentally damaging.In contrast to human-made materials, organisms synthesize composites whose intricate structures are more controlled at each scale and hierarchical order.

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