Cyclodextrin polymer coatings resist protein fouling, mammalian cell adhesion, and bacterial attachment

AbstractUndesired attachment of proteins, cells/bacteria, and organisms on material surfaces is problematic in industrial and health care settings. In this study, polymer coatings are synthesized from subunits of cyclodextrin, an additive/excipient found in food/pharmaceutical formulations. These unique polymers, which have been applied mainly towards sustained drug delivery applications, are evaluated in this study for their ability to mitigate non-specific protein adsorption, mammalian cell (NIH/3T3) adhesion, and bacterial cell (Staphylococcus aureus, Escherichia coli) attachment. Effects of cyclodextrin polymer composition, particularly incorporation of nonpolar crosslinks, on material properties and passive anti-biofouling performance are investigated. Results suggest that lightly-crosslinked cyclodextrin polymers possess excellent passive resistance to protein, cell, and bacterial attachment, likely due to the hydrophilic and electrically neutral surface properties of these coatings. At the same time, anti-biofouling performance decreased with increasing crosslink ratios, possibly a reflection of decreased polymer mobility, increased rigidity, and increased hydrophobic character. Cyclodextrin-based materials may be broadly useful as coatings in industrial or medical applications where biofouling-resistant and/or drug-delivering surfaces are required.

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Tuning the Density of Poly(ethylene glycol) Chains to Control Mammalian Cell and Bacterial Attachment

Polymers ◽

10.3390/polym9080343 ◽

2017 ◽

Vol 9 (12) ◽

pp. 343 ◽

Cited By ~ 9

Author(s):

Ahmed Al-Ani ◽

Hitesh Pingle ◽

Nicholas P Reynolds ◽

Peng-Yuan Wang ◽

Peter Kingshott

Keyword(s):

Ethylene Glycol ◽

Mammalian Cell ◽

Bacterial Attachment ◽

Poly Ethylene Glycol ◽

Poly Ethylene

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A novel mechanism-based mammalian cell assay for the identification of SH2-domain-specific protein-protein inhibitors

Chemistry & Biology ◽

10.1016/s1074-5521(98)90112-0 ◽

1998 ◽

Vol 5 (10) ◽

pp. 529-538 ◽

Cited By ~ 7

Author(s):

Richard J. Rickles ◽

Pamela A. Henry ◽

Wei Guan ◽

Mihai Azimioara ◽

William C. Shakespeare ◽

...

Keyword(s):

Mammalian Cell ◽

Specific Protein ◽

Sh2 Domain ◽

Protein Inhibitors ◽

Domain Specific ◽

Cell Assay

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A specific protein, p92, detected in flat revertants derived from NIH/3T3 transformed by human activated c-Ha-ras oncogene

Experimental Cell Research ◽

10.1016/0014-4827(90)90217-x ◽

1990 ◽

Vol 186 (1) ◽

pp. 115-121 ◽

Cited By ~ 13

Author(s):

Hisakazu Fujita ◽

Hiroaki Suzuki ◽

Noboru Kuzumaki ◽

Leonhard Müllauer ◽

Yoshifumi Ogiso ◽

...

Keyword(s):

Specific Protein ◽

Ras Oncogene ◽

Nih 3T3

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mDia2 Induces the Actin Scaffold for the Contractile Ring and Stabilizes Its Position during Cytokinesis in NIH 3T3 Cells

Molecular Biology of the Cell ◽

10.1091/mbc.e07-10-1086 ◽

2008 ◽

Vol 19 (5) ◽

pp. 2328-2338 ◽

Cited By ~ 124

Author(s):

Sadanori Watanabe ◽

Yoshikazu Ando ◽

Shingo Yasuda ◽

Hiroshi Hosoya ◽

Naoki Watanabe ◽

...

Keyword(s):

Rna Interference ◽

Mammalian Cell ◽

Equatorial Region ◽

Cleavage Furrow ◽

3T3 Cells ◽

Contractile Ring ◽

Binucleate Cells ◽

Dividing Cells ◽

Nih 3T3 ◽

Nih 3T3 Cells

mDia proteins are mammalian homologues of Drosophila diaphanous and belong to the formin family proteins that catalyze actin nucleation and polymerization. Although formin family proteins of nonmammalian species such as Drosophila diaphanous are essential in cytokinesis, whether and how mDia proteins function in cytokinesis remain unknown. Here we depleted each of the three mDia isoforms in NIH 3T3 cells by RNA interference and examined this issue. Depletion of mDia2 selectively increased the number of binucleate cells, which was corrected by coexpression of RNAi-resistant full-length mDia2. mDia2 accumulates in the cleavage furrow during anaphase to telophase, and concentrates in the midbody at the end of cytokinesis. Depletion of mDia2 induced contraction at aberrant sites of dividing cells, where contractile ring components such as RhoA, myosin, anillin, and phosphorylated ERM accumulated. Treatment with blebbistatin suppressed abnormal contraction, corrected localization of the above components, and revealed that the amount of F-actin at the equatorial region during anaphase/telophase was significantly decreased with mDia2 RNAi. These results demonstrate that mDia2 is essential in mammalian cell cytokinesis and that mDia2-induced F-actin forms a scaffold for the contractile ring and maintains its position in the middle of a dividing cell.

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Study on Adhesion and Biocompatibility of Copolymer of MMA-BMA-MAA for Coronary Stents Coatings

Materials Science Forum ◽

10.4028/www.scientific.net/msf.475-479.2395 ◽

2005 ◽

Vol 475-479 ◽

pp. 2395-2398 ◽

Cited By ~ 2

Author(s):

Li-Li Tan ◽

Ke Yang ◽

Bing Chun Zhang ◽

Yong Liang

Keyword(s):

Methyl Methacrylate ◽

Methacrylic Acid ◽

Polymer Coatings ◽

Metal Substrate ◽

Coronary Stents ◽

Polymer Composition ◽

Butyl Methacrylate ◽

Purification Method ◽

Adhesion Properties ◽

Copolymer Coating

Implantation of drug releasing coronary stents has been developed as an useful method for prevention of the restenosis in blood vessels. Copolymers of methyl methacrylate (MMA), butyl methacrylate (BMA) and methacrylic acid (MAA) were prepared as the coating polymer for drug releasing stent. The adhesion of the polymer with metal substrate and the biocompatibility were studied. Effect of the polymer composition on the adhesion between polymer coatings and metal substrate, as well as the effect of the polymer purification method on biocompatibility of the polymer, was studied. The results showed that the copolymer coating has good dry and wet adhesion properties, which can be improved by control of the polymer compositions. Biocompatibility of the prepared copolymer comes within the permission limit.

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Plk is an M-phase-specific protein kinase and interacts with a kinesin-like protein, CHO1/MKLP-1.

Molecular and Cellular Biology ◽

10.1128/mcb.15.12.7143 ◽

1995 ◽

Vol 15 (12) ◽

pp. 7143-7151 ◽

Cited By ~ 181

Author(s):

K S Lee ◽

Y L Yuan ◽

R Kuriyama ◽

R L Erikson

Keyword(s):

Protein Kinase ◽

Kinase Activity ◽

Maximum Level ◽

Specific Protein ◽

Cyclin B ◽

Cdc2 Kinase ◽

M Phase ◽

Nih 3T3

PLK (STPK13) encodes a murine protein kinase closely related to those encoded by the Drosophila melanogaster polo gene and the Saccharomyces cerevisiae CDC5 gene, which are required for normal mitotic and meiotic divisions. Affinity-purified antibody generated against the C-terminal 13 amino acids of Plk specifically recognizes a single polypeptide of 66 kDa in MELC, NIH 3T3, and HeLa cellular extracts. The expression levels of both poly(A)+ PLK mRNA and its encoded protein are most abundant about 17 h after serum stimulation of NIH 3T3 cells. Plk protein begins to accumulate at the S/G2 boundary and reaches the maximum level at the G2/M boundary in continuously cycling cells. Concurrent with cyclin B-associated cdc2 kinase activity, Plk kinase activity sharply peaks at the onset of mitosis. Plk enzymatic activity gradually decreases as M phase proceeds but persists longer than cyclin B-associated cdc2 kinase activity. Plk is localized to the area surrounding the chromosomes in prometaphase, appears condensed as several discrete bands along the spindle axis at the interzone in anaphase, and finally concentrates at the midbody during telophase and cytokinesis. Plk and CHO1/mitotic kinesin-like protein 1 (MKLP-1), which induces microtubule bundling and antiparallel movement in vitro, are colocalized during late M phase. In addition, CHO1/MKLP-1 appears to interact with Plk in vivo and to be phosphorylated by Plk-associated kinase activity in vitro.

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Structure and Properties of Heterocomposite Polymeric Materials and Coatings from them Obtained by Heliotechnological Method

International Journal of Recent Technology and Engineering - 2 ◽

10.35940/ijrte.c1085.1083s19 ◽

2019 ◽

Vol 8 (3S) ◽

pp. 399-402

Keyword(s):

Polymeric Materials ◽

High Viscosity ◽

Polymer Coatings ◽

Polymer Materials ◽

Polymer Composition ◽

Process Equipment ◽

Practical Implementation ◽

Structure And Properties ◽

Hydroabrasive Wear ◽

Composite Casting

The paper presents the results of the study of the creation of new hetero-composite casting polymer materials and coatings from them. The effect of ambient temperature on microhardness and impact strength of polymer coatings is studied. It has been found that treatment of the polymer composition with solar radiation is an effective way of controlling the curing reaction, saving energy in the preparation of high viscosity epoxy compositions, which is important for their practical implementation in the industry for process equipment and products operating under conditions of corrosive hydroabrasive wear.

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Signal transduction in the mammalian cell during bacterial attachment and entry

Cell ◽

10.1016/0092-8674(93)90270-z ◽

1993 ◽

Vol 73 (5) ◽

pp. 903-920 ◽

Cited By ~ 237

Author(s):

James B. Bliska ◽

Jorge E. Galán ◽

Stanley Falkow

Keyword(s):

Signal Transduction ◽

Mammalian Cell ◽

Bacterial Attachment

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Abstract 130: Developing Optogenetic Tools for Mitochondria and sarco/endoplasmic Reticulum Interactions

Circulation Research ◽

10.1161/res.121.suppl_1.130 ◽

2017 ◽

Vol 121 (suppl_1) ◽

Author(s):

Masayuki Yazawa ◽

Fan Shi

Keyword(s):

Endoplasmic Reticulum ◽

Calcium Signaling ◽

Mammalian Cell ◽

Molecular Mechanisms ◽

Calcium Handling ◽

3T3 Cells ◽

Mammalian Cell Lines ◽

Viral Transduction ◽

Nih 3T3 ◽

Human And Mouse

Interaction between mitochondria and sarco/endoplasmic reticulum (SR/ER) is essential for cardiac calcium handling, metabolism and autophagy. Here we present novel light-induced systems to control the interaction between mitochondria and SR/ER in mammalian cardiomyocytes. To develop this system, first we have tested and optimized multiple light-induced hetero dimer domains in mammalian cell lines, HEK 293T and NIH 3T3 cells. Using electron and confocal microscopes with an optimized system, next we confirm that blue light (~450-470nm, shot illumination ~1sec) could induce coupling of SR/ER with mitochondria. In addition, we developed reversible and non-reversible types of light-induced coupling of mitochondria and SR/ER for further applications in human and mouse cardiomyocytes with viral transduction. The new optogenetic tools will help us study the molecular mechanisms underlying cardiac calcium signaling, metabolism and autophagy.

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