scholarly journals Gradient of Tactile Properties in the Rat Whisker Pad

2020 ◽  
Author(s):  
Erez Gugig ◽  
Hariom Sharma ◽  
Rony Azouz
Keyword(s):  
Object Localization ◽  
Neuronal Responses ◽  
Systematic Map ◽  
Tactile Information ◽  
First Order ◽  
Sensing System ◽  
Whisker Length ◽  
Whisker Pad ◽  
Higher Sensitivity

AbstractThe array of vibrissae on a rat’s face is the first stage in a high-resolution tactile sensing system. Progressing from rostral to caudal in any vibrissae row results in an increase in whisker length and thickness. This in turn may provide a systematic map of separate tactile channels governed by the mechanical properties of the whiskers. To examine whether this map is expressed in a location-dependent transformation of tactile signals into whisker vibrations and neuronal responses, we monitored whiskers’ movements across various surfaces and edges. We found a robust rostral-caudal gradient of tactile information transmission in which rostral shorter vibrissae displayed a higher sensitivity and a bigger differences in response to the different textures, whereas longer caudal vibrissae were less sensitive to the different textures. Nonetheless, we found that texture identity is not represented spatially across the whisker pad. Based on the responses of first-order sensory neurons, we found that neurons innervating rostral whiskers were better suited for textures discrimination, whereas neurons innervating caudal whiskers were more suited for edge detection. To examine the functional role of this organization, we monitored the whisking activity of awake rats foraging for food. We found a caudal-rostral gradient of whisking angle movements in which the longer caudal vibrissae spanned a larger space making them more suitable for object localization, whereas the rostral shorter vibrissae hardly moved. These results suggest that the whisker array in rodents forms a sensory structure in which different facets of tactile information are transmitted through location-dependent gradient of vibrissae on the rat’s face.

Exploring Baryon Rich Matter with Heavy-Ion Collisions

2019 ◽  
Vol 64 (7) ◽  
pp. 583 ◽  
Author(s):  
S. Harabasz
Keyword(s):  
Center Of Mass ◽  
Heavy Ion ◽  
State Density ◽  
Heavy Nuclei ◽  
First Order ◽  
Center Of Mass Energy ◽  
Ion Collisions ◽  
Deconfinement Phase Transition ◽  
Ground State Density

Collisions of heavy nuclei at (ultra-)relativistic energies provide a fascinating opportunity to re-create various forms of matter in the laboratory. For a short extent of time (10-22 s), matter under extreme conditions of temperature and density can exist. In dedicated experiments, one explores the microscopic structure of strongly interacting matter and its phase diagram. In heavy-ion reactions at SIS18 collision energies, matter is substantially compressed (2–3 times ground-state density), while moderate temperatures are reached (T < 70 MeV). The conditions closely resemble those that prevail, e.g., in neutron star mergers. Matter under such conditions is currently being studied at the High Acceptance DiElecton Spectrometer (HADES). Important topics of the research program are the mechanisms of strangeness production, the emissivity of matter, and the role of baryonic resonances herein. In this contribution, we will focus on the important experimental results obtained by HADES in Au+Au collisions at 2.4 GeV center-of-mass energy. We will also present perspectives for future experiments with HADES and CBM at SIS100, where higher beam energies and intensities will allow for the studies of the first-order deconfinement phase transition and its critical endpoint.

scholarly journals A9 DORSAL ROOT GANGLIA NEURONAL RESPONSES AND SUBSTANCE P PRODUCTION ARE HIGHER IN MALE MICE

2021 ◽  
Vol 4 (Supplement_1) ◽  
pp. 10-11
Author(s):  
J Pujo ◽  
G De Palma ◽  
J Lu ◽  
S M Collins ◽  
P Bercik
Keyword(s):  
Abdominal Pain ◽  
Substance P ◽  
Gut Microbiota ◽  
Sensory Neurons ◽  
Dorsal Root ◽  
Neuronal Response ◽  
Neuronal Responses ◽  
Visceral Sensitivity ◽  
Germ Free

Abstract Background Abdominal pain is a common complaint in patients with chronic gastrointestinal disorders. Accumulating evidence suggests that gut microbiota is an important determinant of gut function, including visceral sensitivity. Germ-free (GF) mice have been shown to display visceral hypersensitivity, which normalizes after colonization. Sex also appears to play a key role in visceral sensitivity, as women report more abdominal pain than men. Thus, both gut bacteria and sex are important in the regulation of gut nociception, but the underlying mechanisms remain poorly understood. Aims To investigate the role of gut microbiota and sex in abdominal pain. Methods We used primary cultures of sensory neurons from dorsal root ganglia (DRG) of female and male conventionally raised (SPF) or germ-free (GF) mice (7–18 weeks old). To study the visceral afferent activity in vitro, calcium mobilization in DRG sensory neurons was measured by inverted fluorescence microscope using a fluorescent calcium probe Fluo-4 (1mM). Two parameters were considered i) the percentage of responding neurons ii) the intensity of the neuronal response. First, DRG sensory neurons were stimulated by a TRPV1 agonist capsaicin (12.5nM, 125nM and 1.25µM) or by a mixture of G-protein coupled receptors agonist (GPCR: bradykinin, histamine and serotonin; 1µM, 10µM and 100µM). We next measured the neuronal production of substance P and calcitonin gene-related peptide (CGRP), two neuropeptides associated with nociception, in response to capsaicin (1.25µM) or GPCR agonists (100µM) by ELISA and EIA, respectively. Results The percentage of neurons responding to capsaicin and GPCR agonists was similar in male and female SPF and GF mice. However, the intensity of the neuronal response was higher in SPF male compared to SPF female in response to capsaicin (125nM: p=0.0336; 1.25µM: p=0.033) but not to GPCR agonists. Neuronal activation was similar in GF and SPF mice of both sexes after administration of capsaicin or GPCR agonists. Furthermore, substance P and CGRP production by sensory neurons induced by capsaicin or GPCR agonists was similar in SPF and GF mice, regardless of sex. However, while the response to capsaicin was similar, the GPCR agonists-induced production of substance P was higher in SPF male mice compared to SPF females (p=0.003). The GPCR agonists-induced production of CGRP was similar in SPF male and female mice. Conclusions Our data suggest that at the level of DRG neurons, the absence of gut microbiota does not predispose to visceral hypersensitivity. The intensity of DRG neuronal responses to capsaicin and the GPCR agonists-induced production of substance P are higher in male compared to female mice, in contrast to previously published studies in various models of acute and chronic pain. Further studies are thus needed to investigate the role of sex in visceral sensitivity. Funding Agencies CIHR

scholarly journals Ganglioside incorporation and release by the isolated perfused rat liver

1991 ◽  
Vol 274 (2) ◽  
pp. 581-585 ◽  
Author(s):  
S C Kivatinitz ◽  
A Miglio ◽  
R Ghidoni
Keyword(s):  
Rat Liver ◽  
The Other ◽  
Regulatory Role ◽  
Isolated Perfused Rat Liver ◽  
Order Kinetic ◽  
Perfused Rat Liver ◽  
Ganglioside Gm1 ◽  
First Order ◽  
Pseudo First Order

The fate of exogenous ganglioside GM1 labelled in the sphingosine moiety, [Sph-3H]GM1, administered as a pulse, in the isolated perfused rat liver was investigated. When a non-recirculating protocol was employed, the amount of radioactivity in the liver and perfusates was found to be dependent on the presence of BSA in the perfusion liquid and on the time elapsed after the administration of the ganglioside. When BSA was added to the perfusion liquid, less radioactivity was found in the liver and more in the perfusate at each time tested, for up to 1 h. The recovery of radioactivity in the perfusates followed a complex course which can be described by three pseudo-first-order kinetic constants. The constants, in order of decreasing velocity, are interpreted as: (a) the dilution of the labelled GM1 by the constant influx of perfusion liquid; (b) the washing off of GM1 loosely bound to the surface of liver cells; (c) the release of gangliosides from the liver. Process (b) was found to be faster in the presence of BSA, probably owing to the ability of BSA to bind gangliosides. The [Sph-3H]GM1 in the liver underwent metabolism, leading to the appearance of products of anabolic (GD1a, GD1b) and catabolic (GM2, GM3) origin; GD1a appeared before GM2 and GM3 but, at times longer than 10 min, GM2 and GM3 showed more radioactivity than GD1a. At a given time the distribution of the radioactivity in the perfusates was quite different from that of the liver. In fact, after 60 min GD1a was the only metabolite present in any amount, the other being GM3, the quantity of which was small. This indicates that the liver is able to release newly synthesized gangliosides quite specifically. When a recirculating protocol was used, there were more catabolites and less GD1a than with the non-recirculating protocol. A possible regulatory role of ganglioside re-internalization on their own metabolism in the liver is postulated.

Olfactory modulation of barrel cortex activity during active whisking and passive whisker stimulation

2021 ◽  
Author(s):  
Anthony Renard ◽  
Evan Harrell ◽  
Brice Bathallier
Keyword(s):  
Facial Nerve ◽  
Calcium Imaging ◽  
Tactile Stimulation ◽  
Barrel Cortex ◽  
Neuronal Responses ◽  
Population Activity ◽  
Tactile Information ◽  
Large Populations ◽  
Whisker Stimulation ◽  
The Impact

Abstract Rodents depend on olfaction and touch to meet many of their fundamental needs. The joint significance of these sensory systems is underscored by an intricate coupling between sniffing and whisking. However, the impact of simultaneous olfactory and tactile inputs on sensory representations in the cortex remains elusive. To study these interactions, we recorded large populations of barrel cortex neurons using 2-photon calcium imaging in head-fixed mice during olfactory and tactile stimulation. We find that odors alter barrel cortex activity in at least two ways, first by enhancing whisking, and second by central cross-talk that persists after whisking is abolished by facial nerve sectioning. Odors can either enhance or suppress barrel cortex neuronal responses, and while odor identity can be decoded from population activity, it does not interfere with the tactile representation. Thus, barrel cortex represents olfactory information which, in the absence of learned associations, is coded independently of tactile information.

Neuronal responses to static texture patterns in area V1 of the alert macaque monkey

1992 ◽  
Vol 67 (4) ◽  
pp. 961-980 ◽  
Author(s):  
J. J. Knierim ◽  
D. C. van Essen
Keyword(s):  
Contrast Effect ◽  
Macaque Monkey ◽  
Average Difference ◽  
Contrast Effects ◽  
Neuronal Responses ◽  
Suppression Effect ◽  
Orientation Contrast ◽  
Classical Receptive Field ◽  
Oriented Line

1. We recorded responses from neurons in area V1 of the alert macaque monkey to textured patterns modeled after stimuli used in psychophysical experiments of pop-out. Neuronal responses to a single oriented line segment placed within a cell's classical receptive field (CRF) were compared with responses in which the center element was surrounded by rings of elements placed entirely outside the CRF. The orientations of the surround elements either matched the center element, were orthogonal to it, or were random. 2. The addition of the textured surround tended to suppress the response to the center element by an average of 34%. Overall, almost 80% of the 122 cells analyzed in detail were significantly suppressed by at least one of the texture surrounds. 3. Cells tended to respond more strongly to a stimulus in which there was a contrast in orientation between the center and surround than to a stimulus lacking such contrast. The average difference was 9% of the response to the optimally oriented center element alone. For the 32% of the cells showing a statistically significant orientation contrast effect, the average difference was 28%. 4. Both the general suppression and orientation contrast effects originated from surround regions at the ends of the center bar as well as regions along the sides of the center bar. 5. The amount of suppression induced by the texture surround decreased as the density of the texture elements decreased. 6. Both the general suppression and the orientation contrast effects appeared early in the population response to the stimuli. The general suppression effect took approximately 7 ms to develop, whereas the orientation contrast effect took 18-20 ms to develop. 7. These results are consistent with a possible functional role of V1 cells in the mediation of perceptual pop-out and in the segregation of texture borders. Possible anatomic substrates of the effects are discussed.

The Propagation Mechanism of a Vortex on the β Plane

2007 ◽  
Vol 37 (9) ◽  
pp. 2316-2330 ◽  
Author(s):  
Peter Jan van Leeuwen
Keyword(s):  
Coriolis Force ◽  
Opposite Sign ◽  
Propagation Mechanism ◽  
Reduced Gravity ◽  
Background Current ◽  
Background Flow ◽  
Coriolis Parameter ◽  
First Order ◽  
Flow Changes

Abstract The propagation velocity and propagation mechanism for vortices on a β plane are determined for a reduced-gravity model by integrating the momentum equations over the β plane. Isolated vortices, vortices in a background current, and initial vortex propagation from rest are studied. The propagation mechanism for isolated anticyclones as well as cyclones, which has been lacking up to now, is presented. It is shown that, to first order, the vortex moves to generate a Coriolis force on the mass anomaly of the vortex to compensate for the force on the vortex due to the variation of the Coriolis parameter. Only the mass anomaly of the vortex is of importance, because the Coriolis force due to the motion of the bulk of the layer moving with the vortex is almost fully compensated by the Coriolis force on the motion of the exterior flow. Because the mass anomaly of a cyclone is negative the force and acceleration have opposite sign. The role of dipolar structures in steadily moving vortices is discussed, and it is shown that their overall structure is fixed by the steady westward motion of the mass anomaly. Furthermore, it is shown that reduced-gravity vortices are not advected with a background flow. The reason for this behavior is that the background flow changes the ambient vorticity gradient such that the vortex obtains an extra self-propagation term that exactly cancels the advection by the background flow. Last, it is shown that a vortex initially at rest will accelerate equatorward first, after which a westward motion is generated. This result is independent of the sign of the vortex.

On the role of Ramsey quantifiers in first order arithmetic

1982 ◽  
Vol 47 (2) ◽  
pp. 423-435 ◽  
Author(s):  
James H. Schmerl ◽  
Stephen G. Simpson
Keyword(s):  
Formal System ◽  
Intended Meaning ◽  
Completeness Proof ◽  
First Order ◽  
Uncountable Cardinal ◽  
Consistent Extension ◽  
Witness Set ◽  
Order Arithmetic ◽  
Infinite Set

The purpose of this paper is to study a formal system PA(Q2) of first order Peano arithmetic, PA, augmented by a Ramsey quantifier Q2 which binds two free variables. The intended meaning of Q2xx′φ(x, x′) is that there exists an infinite set X of natural numbers such that φ(a, a′) holds for all a, a′ Є X such that a ≠ a′. Such an X is called a witness set for Q2xx′φ(x, x′). Our results would not be affected by the addition of further Ramsey quantifiers Q3, Q4, …, Here of course the intended meaning of Qkx1 … xkφ(x1,…xk) is that there exists an infinite set X such that φ(a1…, ak) holds for all k-element subsets {a1, … ak} of X.Ramsey quantifiers were first introduced in a general model theoretic setting by Magidor and Malitz [13]. The system PA{Q2), or rather, a system essentially equivalent to it, was first defined and studied by Macintyre [12]. Some of Macintyre's results were obtained independently by Morgenstern [15]. The present paper is essentially self-contained, but all of our results have been directly inspired by those of Macintyre [12].After some preliminaries in §1, we begin in §2 by giving a new completeness proof for PA(Q2). A by-product of our proof is that for every regular uncountable cardinal k, every consistent extension of PA(Q2) has a k-like model in which all classes are definable. (By a class we mean a subset of the universe of the model, every initial segment of which is finite in the sense of the model.)

Role of Single Word Variables in Recall of Statistical Approximations to English

1966 ◽  
Vol 19 (2) ◽  
pp. 627-634 ◽  
Author(s):  
Mari J. K. Brown
Keyword(s):  
Free Recall ◽  
Higher Order ◽  
Order Of Approximation ◽  
Single Word ◽  
First Order ◽  
Original Order ◽  
Sequential Organization ◽  
The Individual

Free recall of lists at different orders of approximation to English was compared to the recall of the same lists when the order of the words had been scrambled to destroy their sequential organization. Recall of the organized lists showed the typical improvement with increasing order of approximation. Recall of the scrambled lists was unrelated to the original order of approximation. The results indicate that increased recall with increasing order of approximation to English is not produced by systematic differences in the characteristics of the individual words comprising the approximations. When recall of the organized lists was scored in terms of the number of longer sequences present in recall, the number of recalled sequences of any given length increased as order of approximation to English increased, with the first order list showing proportionally less organization in recall than the second and higher order lists.

scholarly journals Reasoning with Time Intervals: A Logical and Computational Perspective

2012 ◽  
Vol 2012 ◽  
pp. 1-19 ◽  
Author(s):  
Guido Sciavicco
Keyword(s):  
Temporal Reasoning ◽  
Point Of View ◽  
Time Intervals ◽  
First Order ◽  
Modal Interval ◽  
The Family ◽  
First Results ◽  
Comprehensive Survey ◽  
Classical Point

The role of time in artificial intelligence is extremely important. Interval-based temporal reasoning can be seen as a generalization of the classical point-based one, and the first results in this field date back to Hamblin (1972) and Benhtem (1991) from the philosophical point of view, to Allen (1983) from the algebraic and first-order one, and to Halpern and Shoham (1991) from the modal logic one. Without purporting to provide a comprehensive survey of the field, we take the reader to a journey through the main developments in modal and first-order interval temporal reasoning over the past ten years and outline some landmark results on expressiveness and (un)decidability of the satisfiability problem for the family of modal interval logics.

scholarly journals SM22α Loss Contributes to Apoptosis of Vascular Smooth Muscle Cells via Macrophage-Derived circRasGEF1B

2021 ◽  
Vol 2021 ◽  
pp. 1-20
Author(s):  
Pin Lv ◽  
Ya-Juan Yin ◽  
Peng Kong ◽  
Li Cao ◽  
Hao Xi ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Abdominal Aortic Aneurysm ◽  
Aortic Aneurysm ◽  
Vascular Smooth Muscle ◽  
Inflammatory Cell ◽  
Activated Macrophages ◽  
Abdominal Aortic ◽  
Specific Manner ◽  
Higher Sensitivity

Vascular smooth muscle cell (VSMC) apoptosis is a major defining feature of abdominal aortic aneurysm (AAA) and mainly caused by inflammatory cell infiltration. Smooth muscle (SM) 22α prevents AAA formation through suppressing NF-κB activation. However, the role of SM22α in VSMC apoptosis is controversial. Here, we identified that SM22α loss contributed to apoptosis of VSMCs via activation of macrophages. Firstly, deficiency of SM22α enhanced the interaction of VSMCs with macrophages. Macrophages were retained and activated by Sm22α-/- VSMCs via upregulating VCAM-1 expression. The ratio of apoptosis was increased by 1.62-fold in VSMCs treated with the conditional media (CM) from activated RAW264.7 cells, compared to that of the control CM ( P < 0.01 ), and apoptosis of Sm22α-/- VSMCs was higher than that of WT VSMCs ( P < 0.001 ). Next, circRasGEF1B from activated macrophages was delivered into VSMCs promoting ZFP36 expression via stabilization of ZFP36 mRNA. Importantly, circRasGEF1B, as a scaffold, guided ZFP36 to preferentially bind to and decay Bcl-2 mRNA in a sequence-specific manner and triggered apoptosis of VSMCs, especially in Sm22α-/- VSMCs. These findings reveal a novel mechanism by which the circRasGEF1B-ZFP36 axis mediates macrophage-induced VSMC apoptosis via decay of Bcl-2 mRNA, whereas Sm22α-/- VSMCs have a higher sensitivity to apoptosis.

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