scholarly journals User guide to the red blood cell model (RCM), a multiplatform JAVA-based model of human red blood cell homeostasis

2020 ◽  
Author(s):  
Simon Rogers ◽  
Virgilio L. Lew
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Cell Model ◽  
Complex Processes ◽  
Wide Range ◽  
Experimental Protocols ◽  
Health And Disease ◽  
Cell Dehydration ◽  
Wide Group ◽  
Protein Crowding

AbstractWe introduce here a new multiplatform JAVA-based mathematical-computational model of RBC homeostasis for investigating the dynamics of changes in RBC homeostasis in health and disease. We provide a brief overview on the homeostasis of human RBCs and on the general biophysical principles guiding the modelling design. By way of a detailed tutorial we apply the model to investigate in depth the multiple effects associated with RBC dehydration induced by potassium permeabilization, a necessary preliminary for understanding the pathophysiology of a wide group of inherited haemolytic anaemias, a subject of intense current research and clinical interest. Using the red cell model (RCM), we design and run in silico representations of experimental protocols to study global RBC responses to calcium and potassium permeabilization covering a wide range of experimental, physiological and pathological conditions. Model outputs report the evolution in time of all the homeostatic variables in the system allowing, for the first time, a detailed and comprehensive account of the complex processes shaping global cell responses. Analysis of the results explains the mechanisms by which the entangled operation of all the RBC components link cell dehydration and protein crowding to cell acidification and to the generation of hypertonic, alkaline effluents. Open access to the RCM in a GitHub repository, together with the tutorial primed for a specific investigation pave the way for researchers and clinicians to apply the model on many different aspects of RBC physiology and pathology.

A Red Blood Cell Model to Estimate the Hemolysis Fingerprint of Cardiovascular Devices

2017 ◽  
Vol 42 (1) ◽  
pp. 58-67 ◽  
Author(s):  
Riccardo Toninato ◽  
Giuseppe Fadda ◽  
Francesca Maria Susin
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Cell Model ◽  
Cardiovascular Devices

scholarly journals Numerical simulations of blood cell flow in non-Newtonian fluid

2020 ◽  
Vol 306 ◽  
pp. 01006
Author(s):  
Kazuhiro Shitara ◽  
Toru Hyakutake
Keyword(s):  
Blood Cell ◽  
Red Blood Cells ◽  
Newtonian Fluid ◽  
Red Blood Cell ◽  
Blood Cells ◽  
Cell Model ◽  
Flow Behavior ◽  
Shear Thinning ◽  
Mutual Interference ◽  
Axial Concentration

We investigated how non-Newtonian viscosity behavior affects the flow characteristics of blood cells. Our findings offer insight about how shear thinning affects the dispersion of liposome-encapsulated hemoglobin and red blood cells in blood. The lattice Boltzmann method was used for fluid calculations, and the rheological properties of the non-Newtonian fluid were modeled with power-law relationships. The deformable three-dimensional red blood cell model was applied. First, we investigated the effects of shear thinning on the flow behavior of single blood cell. Simulation results indicate that shear thinning promotes the axial concentration of red blood cells. Next, varied the hematocrit to see how mutual interference between blood cells affects flow. At low hematocrit, shear thinning clearly promotes the axial concentration of red blood cells. As the hematocrit increases, in contrast, mutual interference has a greater effect, which counteracts shear thinning so the red blood cell distribution resembles the distribution within a Newtonian fluid.

Mechanical response of human red blood cells in health and disease: Some structure-property-function relationships

2006 ◽  
Vol 21 (8) ◽  
pp. 1871-1877 ◽  
Author(s):  
S. Suresh
Keyword(s):  
Molecular Structure ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Mechanical Response ◽  
Structure Property ◽  
Human Red Blood Cells ◽  
Health And Disease ◽  
Cell Elasticity ◽  
Altered Cell ◽  
Structure Properties

Aspects of mechanical deformability and biorheology of the human red blood cell are known to play a pivotal role in influencing organ function as well as states of overall health and disease. In this article, consequences of alterations to the membrane and cytoskeletal molecular structure of the human red blood cell are considered in the context of an infectious disease,Plasmodium falciparummalaria, and several hereditary hemolytic disorders: spherocytosis, elliptocytosis, and sickle cell anemia. In each of these cases, the effects of altered cell shape or molecular structure on cell elasticity, motility, and biorheology are examined. These examples are used to gain broad perspectives on the connections among cell and subcellular structure, properties, and disease at the intersections of engineering, biology, and medicine.

scholarly journals Exploring the gating mechanisms of aquaporin-3: new clues for the design of inhibitors?

2016 ◽  
Vol 12 (5) ◽  
pp. 1564-1573 ◽  
Author(s):  
A. de Almeida ◽  
A. P. Martins ◽  
A. F. Mósca ◽  
H. J. Wijma ◽  
C. Prista ◽  
...  
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Cell Model ◽  
Aquaporin 3 ◽  
Gating Mechanisms ◽  
First Time

The pH gating of human AQP3 and its effects on both water and glycerol permeabilities have been fully characterized for the first time using a human red blood cell model (hRBC).

scholarly journals Probing red blood cell mechanics, rheology and dynamics with a two-component multi-scale model

2014 ◽  
Vol 372 (2021) ◽  
pp. 20130389 ◽  
Author(s):  
Xuejin Li ◽  
Zhangli Peng ◽  
Huan Lei ◽  
Ming Dao ◽  
George Em Karniadakis
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Cell Model ◽  
Microfluidic Channel ◽  
Scale Model ◽  
Cross Sectional ◽  
Whole Cell ◽  
Multi Scale ◽  
Rbc Membrane ◽  
Two Component

This study is partially motivated by the validation of a new two-component multi-scale cell model we developed recently that treats the lipid bilayer and the cytoskeleton as two distinct components. Here, the whole cell model is validated and compared against several available experiments that examine red blood cell (RBC) mechanics, rheology and dynamics. First, we investigated RBC deformability in a microfluidic channel with a very small cross-sectional area and quantified the mechanical properties of the RBC membrane. Second, we simulated twisting torque cytometry and compared predicted rheological properties of the RBC membrane with experimental measurements. Finally, we modelled the tank-treading (TT) motion of a RBC in a shear flow and explored the effect of channel width variation on the TT frequency. We also investigated the effects of bilayer–cytoskeletal interactions on these experiments and our simulations clearly indicated that they play key roles in the determination of cell membrane mechanical, rheological and dynamical properties. These simulations serve as validation tests and moreover reveal the capabilities and limitations of the new whole cell model.

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