scholarly journals Assessing the Involvement of Platelet Degranulation in the Therapeutic Properties of Exosome Derived from Amniotic Epithelial Cells through Enrichment and Interaction Network Analysis

2021 ◽  
Author(s):  
Mahmoud Valizadeh ◽  
Ali Haider Bangash ◽  
Donya Hayati ◽  
Ameneh Jafari ◽  
Hassan Rajabi-Maham
Keyword(s):  
Wound Healing ◽  
Epithelial Cells ◽  
Interaction Network ◽  
Biologically Active ◽  
Network Analyses ◽  
Amniotic Epithelial Cells ◽  
Background Database ◽  
Underlying Mechanisms ◽  
Analysis System ◽  
User Friendly

Platelet degranulation allows the release of large secretable pools of biologically active proteins which are critical in wound healing initiation and angiogenesis. Exosomes, which can transport a diverse suite of macromolecules, derived from amniotic epithelial cells (AEC-Exo) improve wound healing and angiogenesis. However, the underlying mechanisms are still unclear. In this investigation, we performed a user-friendly bioinformatics analysis system to identify association among the angiogenic and wound healing effects of AEC-Exo treatments. To this end, FunRich software was used and linked to the Universal Protein Resource (UniProt) as a background database. Several enrichment analyses, including biological process, cellular component, molecular function, and protein domains were conducted on AEC-Exo proteome. Furthermore, to identify the proteins involved in platelet degranulation and evaluate protein-protein association information, comparative analyses and interaction network analyses were illustrated using the NCBI BioSystems, ExoCarta, and STRING databases. Our results indicated the statistically significant association between the proteome in AEC-Exo, platelet degranulation, and their corresponding processes. Therefore, the involvement of platelet degranulation in AEC-Exo proteins may elucidate the angiogenic and wound-healing effects of AEC-Exo treatments.

scholarly journals Producing Human Amniotic Epithelial Cells-only Membrane for Transplantation

2019 ◽  
Author(s):  
Chenze Xu ◽  
Waqas Ahmed ◽  
Lili Xie ◽  
Yan Peng ◽  
Qizheng Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Epithelial Cells ◽  
Human Amniotic Membrane ◽  
Transcriptional Level ◽  
Type I ◽  
Amniotic Epithelial Cells ◽  
Human Amniotic Epithelial Cells ◽  
Conventional Methods ◽  
Clinical Potential

AbstractHuman amniotic epithelial cells (hAECs), as pluripotent stem cells, have characteristics of immune privilege and great clinical potential. Here, we produced hAECs membrane consisting of single-layer hAECs and basal membrane (BM) of human amniotic membrane (hAM). In conventional methods, hAECs were isolated from hAM by repeated trypsin digestion. In this study, collagenase I and cell scraper were used to remove human amniotic mesenchymal stem cells (hAMSCs) from hAM and hAECs-only membranes were produced. These hAECs on the membranes were evaluated by surface biomarkers including epithelial cell adhesion molecule (EpCAM), stage-specific embryonic antigen 4 (SSEA4) and endoglin (CD105), transcriptional level of biomarkers including POU class 5 homeobox 1 (OCT4), sex determining region Y-box 2 (SOX2), fibroblast growth factor 4 (FGF4), immunofluorescence of cytokeratin-8 (CK-8), alpha smooth muscle actin (α-SMA) and collagen type I alpha 1 chain (ColA1). Finally, the hAECs membrane were transplanted on skin-removed mice to evaluate its effect on wound healing. In comparison to the hAECs isolated by the conventional methods, the cells isolated by this proposed method had higher purity of hAECs, expressed higher in pluripotency related genes, and maintained an epithelium construction in a long-term culture. In mice application, the hAECs membrane effectively improved the skin wound healing. An efficient method was successfully established to produce hAECs membrane in this work which not only held promise to obtain hAECs in higher purity and quality, but also showed practical clinical potential.

scholarly journals Exosomal MicroRNAs Derived from Human Amniotic Epithelial Cells Accelerate Wound Healing by Promoting the Proliferation and Migration of Fibroblasts

2018 ◽  
Vol 2018 ◽  
pp. 1-10 ◽  
Author(s):  
Bin Zhao ◽  
Xiaodong Li ◽  
Xiaomin Shi ◽  
Xueqin Shi ◽  
Wei Zhang ◽  
...  
Keyword(s):  
Wound Healing ◽  
Epithelial Cells ◽  
Cell Communication ◽  
Rnase A ◽  
Proteinase K ◽  
Amniotic Epithelial Cells ◽  
Human Amniotic Epithelial Cells ◽  
And Migration ◽  
Proliferation And Migration

Previous work in our laboratory demonstrated that exosomes derived from human amniotic epithelial cells (hAECs) accelerated wound healing by promoting the proliferation and migration of fibroblasts. It is reported that exosomes, which are carriers of the microRNAs (miRNAs) and proteins, play an important role in the regulation of cell-to-cell communication. However, it is still unclear precisely which molecule or which group of molecules carried within hAEC-derived exosomes (hAEC-Exos) mediated wound healing. Here, we explored purified hAEC-Exos together with either proteinase K (PROse) or RNase A on the effect of fibroblasts and cutaneous wound healing. Our experiments demonstrated that hAEC-Exos were positive for exosomal markers CD9, CD63, and CD81. Also, we found that hAEC-Exos could be internalized by fibroblasts and then stimulated cell migration and proliferation. However, the promotive effect of hAEC-Exos was abolished by pretreating hAEC-Exos with RNase A, not PROse. Importantly, in vivo wound healing assay showed that local injection of hAEC-Exos or PROse pretreated hAEC-Exos at skin wounds significantly accelerated wound healing. Our findings revealed an important role of exosomal miRNAs in wound healing.

scholarly journals Amniotic Epithelial Cells Accelerate Diabetic Wound Healing by Modulating Inflammation and Promoting Neovascularization

2018 ◽  
Vol 2018 ◽  
pp. 1-10 ◽  
Author(s):  
Yongjun Zheng ◽  
Shiqing Zheng ◽  
Xiaoming Fan ◽  
Li Li ◽  
Yongqiang Xiao ◽  
...  
Keyword(s):  
Wound Healing ◽  
Epithelial Cells ◽  
Therapeutic Potential ◽  
Macrophage Polarization ◽  
M2 Macrophage ◽  
Diabetic Wound ◽  
Macrophage Phenotype ◽  
Amniotic Epithelial Cells ◽  
Diabetic Wound Healing ◽  
Diabetic Wounds

Human amniotic epithelial cells (hAECs) are nontumorigenic, highly abundant, and low immunogenic and possess multipotent differentiation ability, which make them become ideal alternative stem cell source for regenerative medicine. Previous studies have demonstrated the therapeutic potential of hAECs in many tissue repairs. However, the therapeutic effect of hAECs on diabetic wound healing is still unknown. In this study, we injected hAECs intradermally around the full-thickness excisional skin wounds of db/db mice and found that hAECs significantly accelerated diabetic wound healing and granulation tissue formation. To explore the underlying mechanisms, we measured inflammation and neovascularization in diabetic wounds. hAECs could modulate macrophage phenotype toward M2 macrophage, promote switch from proinflammatory status to prohealing status of wounds, and increase capillary density in diabetic wounds. Furthermore, we found that the hAEC-conditioned medium promoted macrophage polarization toward M2 phenotype and facilitated migration, proliferation, and tube formation of endothelial cells through in vitro experiments. Taken together, we first reported that hAECs could promote diabetic wound healing, at least partially, through paracrine effects to regulate inflammation and promote neovascularization.

ultrastructural process of intestinal wound healing

1995 ◽  
Vol 53 ◽  
pp. 1024-1025
Author(s):  
Rick L. Vaughn ◽  
Shailendra K. Saxena ◽  
John G. Sharp
Keyword(s):  
Wound Healing ◽  
Epithelial Cells ◽  
Smooth Muscles ◽  
Microscopic Level ◽  
Light Microscopic Level ◽  
Ileal Mucosa ◽  
Wound Model ◽  
Serosal Surface ◽  
Complex Process ◽  
Orderly Fashion

We have developed an intestinal wound model that includes surgical construction of an ileo-cecal patch to study the complex process of intestinal wound healing. This allows approximation of ileal mucosa to the cecal serosa and facilitates regeneration of ileal mucosa onto the serosal surface of the cecum. The regeneration of ileal mucosa can then be evaluated at different times. The wound model also allows us to determine the rate of intestinal regeneration for a known size of intestinal wound and can be compared in different situations (e.g. with and without EGF and Peyer’s patches).At the light microscopic level it appeared that epithelial cells involved in regeneration of ileal mucosa originated from the enlarged crypts adjacent to the intestinal wound and migrated in an orderly fashion onto the serosal surface of the cecum. The migrating epithelial cells later formed crypts and villi by the process of invagination and evagination respectively. There were also signs of proliferation of smooth muscles underneath the migratory epithelial cells.

SIGN IN Welcome!Log into your account your username your password Forgot your password Privacy policy PASSWORD RECOVERY Recover your password your email Struktol Home New fast measuring method for process optimization of sucrose crystallization New fast measuring method for process optimization of sucrose crystallization

2020 ◽  
pp. 49-52
Author(s):  
Trine Aabo Andersen
Keyword(s):  
Image Analysis ◽  
Process Optimization ◽  
Crystal Size ◽  
Measuring Method ◽  
Sieve Analysis ◽  
Image Analysis System ◽  
Line System ◽  
Non Invasive ◽  
Analysis System ◽  
User Friendly

A new fast measuring method for process optimization of sucrose crystallization using image analysis based on high quality images and algorithms is introduced. With the mobile, non-invasive at-line system all steps of the sucrose crystallization can be measured to determine the crystal size distribution. The image analysis system is easy to operate and is as well an efficient laboratory solution with user-friendly and customized software. In comparison to sieve analysis, image analyses performed with the ParticleTech Solution have been proven to be reliable.

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