Relationship between SW velocity and muscle activation is inconsistent across different muscle types
ABSTRACTThere is an increasing use of shear wave ultrasound elastography to quantify material properties of muscles under various conditions such as muscle length, levels of activation, in healthy and pathological muscle. However, little is known about the variability in shear wave velocity among muscles. The purpose of this study was to assess the variability of the relationship between SWV and activation across muscles with different architecture, specifically pennation angle: biceps brachii (parallel), tibialis anterior (moderately pennate), gastrocnemius (highly pennate). We tested these muscles at matching levels of activation and similar muscle lengths, relative to optimal length, to limit differences in force across muscles. Shear wave velocity-squared increased with higher levels of muscle activation (p<0.001), but the relationship between shear wave velocity-squared and activation was different across all three muscles (p<0.001). This indicates that SWV was inconsistent across muscle types, even when considering similar levels of activation and torque production. Under passive conditions, shear wave velocity-squared covaried with muscle length and was different across muscles (p<0.001), even at shortest lengths, when differences due to passive force were minimal. Variation of shear wave velocity-squared was only partially explained by changes in pennation angle during contraction (p = 0.004, R2 = 0.4). Our findings suggest that the shear wave velocity varies across muscles under passive and active conditions even when relative length is considered and that shear wave velocity may be dependent on other muscle properties, other than pennation angle, that are different between the three muscles.