scholarly journals Isolation of wild yeasts from Olympic National Park and Moniliella megachiliensis ONP131 physiological characterization for beer fermentation

2021 ◽  
Author(s):  
Renan Eugenio Araujo Piraine ◽  
David G. Nickens ◽  
David J. Sun ◽  
Fabio Pereira Leivas Leite ◽  
Matthew L. Bochman
Keyword(s):  
National Park ◽  
Malt Extract ◽  
Physiological Characterization ◽  
Olympic National Park ◽  
Beer Industry ◽  
Wild Yeasts ◽  
High Concentrations ◽  
Different Strains ◽  
Incubation Temperatures ◽  
Hop Acid

Thousands of yeasts have the potential for industrial application, though many were initially considered contaminants in the beer industry. However, these organisms are currently considered important components in beers because they contribute new flavors. Non-Saccharomyces wild yeasts can be important tools in the development of new products, and the objective of this work was to obtain and characterize novel yeast isolates for their ability to produce beer. Wild yeasts were isolated from environmental samples from Olympic National Park and analyzed for their ability to ferment malt extract medium and beer wort. Six different strains were isolated, of which Moniliella megachiliensis ONP131 displayed the highest levels of attenuation during fermentations. We found that M. megachiliensis could be propagated in common yeast media, tolerated incubation temperatures of 37C and a pH of 2.5, and was able to grow in media containing maltose as the sole carbon source. Yeast cultivation was considerably impacted (p<0.05) by lactic acid, ethanol, and high concentrations of maltose, but ONP131 was tolerant to high salinity and hop acid concentrations. This is one of the first physiological characterizations of M. megachiliensis, which has potential for the production of beer and other fermented beverages.

scholarly journals Isolation of wild yeasts from Olympic National Park and Moniliella megachiliensis ONP131 physiological characterization for beer fermentation

2021 ◽  
pp. 103974
Author(s):  
Renan Eugênio Araujo Piraine ◽  
David Gerald Nickens ◽  
David J. Sun ◽  
Fábio Pereira Leivas Leite ◽  
Matthew L. Bochman
Keyword(s):  
National Park ◽  
Beer Fermentation ◽  
Physiological Characterization ◽  
Olympic National Park ◽  
Wild Yeasts

Adapting to climate change at Olympic National Forest and Olympic National Park

2011 ◽  
Author(s):  
Jessica E. Halofsky ◽  
David L. Peterson ◽  
Kathy A. O’Halloran ◽  
Catherine Hawkins Hoffman
Keyword(s):  
Climate Change ◽  
National Park ◽  
National Forest ◽  
Olympic National Park

scholarly journals Isolation, Characterization, and Ecology of Sulfur-Respiring Crenarchaea Inhabiting Acid-Sulfate-Chloride-Containing Geothermal Springs in Yellowstone National Park

2007 ◽  
Vol 73 (20) ◽  
pp. 6669-6677 ◽  
Author(s):  
Eric S. Boyd ◽  
Robert A. Jackson ◽  
Gem Encarnacion ◽  
James A. Zahn ◽  
Trevor Beard ◽  
...  
Keyword(s):  
Yellowstone National Park ◽  
National Park ◽  
Hot Springs ◽  
Lipid Fraction ◽  
Carbon Sources ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Pcr Analysis ◽  
Geothermal Springs ◽  
Physiological Characterization

ABSTRACT Elemental sulfur (S0) is associated with many geochemically diverse hot springs, yet little is known about the phylogeny, physiology, and ecology of the organisms involved in its cycling. Here we report the isolation, characterization, and ecology of two novel, S0-reducing Crenarchaea from an acid geothermal spring referred to as Dragon Spring. Isolate 18U65 grows optimally at 70 to 72°C and at pH 2.5 to 3.0, while isolate 18D70 grows optimally at 81°C and pH 3.0. Both isolates are chemoorganotrophs, dependent on complex peptide-containing carbon sources, S0, and anaerobic conditions for respiration-dependent growth. Glycerol dialkyl glycerol tetraethers (GDGTs) containing four to six cyclopentyl rings were present in the lipid fraction of isolates 18U65 and 18D70. Physiological characterization suggests that the isolates are adapted to the physicochemical conditions of Dragon Spring and can utilize the natural organic matter in the spring as a carbon and energy source. Quantitative PCR analysis of 16S rRNA genes associated with the S0 flocs recovered from several acid geothermal springs using isolate-specific primers indicates that these two populations together represent 17 to 37% of the floc-associated DNA. The physiological characteristics of isolates 18U65 and 18D70 are consistent with their potential widespread distribution and putative role in the cycling of sulfur in acid geothermal springs throughout the Yellowstone National Park geothermal complex. Based on phenotypic and genetic characterization, the designations Caldisphaera draconis sp. nov. and Acidilobus sulfurireducens sp. nov. are proposed for isolates 18U65 and 18D70, respectively.

scholarly journals Survival characteristics of Salmonella enterica serovar Enteritidis in chicken egg albumen

2006 ◽  
Vol 134 (5) ◽  
pp. 967-976 ◽  
Author(s):  
H. KANG ◽  
C. LOUI ◽  
R. I. CLAVIJO ◽  
L. W. RILEY ◽  
S. LU
Keyword(s):  
Salmonella Enterica ◽  
Iron Acquisition ◽  
Foodborne Pathogen ◽  
Human Infection ◽  
Salmonella Enterica Serovar Enteritidis ◽  
Factors Affecting ◽  
Chicken Egg ◽  
Egg Albumen ◽  
High Concentrations ◽  
Incubation Temperatures

Salmonella enterica serovar Enteritidis (SE) is a major foodborne pathogen primarily causing human infection through contaminated chicken eggs. To understand how SE survives in chicken egg albumen, we systematically and quantitatively analysed the survival properties of SE in egg albumen and identified factors affecting its survival. Survival assays of SE in egg indicate that egg albumen restricted the growth of SE. A major factor that controlled SE's growth in egg albumen was iron restriction, since egg albumen supplemented with iron allowed SE to grow, and iron acquisition mutants of SE showed decreased survival in egg albumen. In addition, low pH of albumen, high concentrations of bacteria and low incubation temperatures of bacteria with albumen facilitates the survival of SE. Our results suggest that egg albumen uses multiple mechanisms to control SE including iron limitation, surface interaction and possible enzymatic activities.

Geomorphology of coastal Olympic National Park

2021 ◽  
Author(s):  
Jon Riedel ◽  
Sharon Sarrantonio ◽  
Stephen Dorsch
Keyword(s):  
National Park ◽  
Olympic National Park

Alpine disturbance studies: Olympic national park, USA

1973 ◽  
Vol 5 (1) ◽  
pp. 25-32 ◽  
Author(s):  
Katherine L. Bell ◽  
Lawrence C. Bliss
Keyword(s):  
National Park ◽  
Olympic National Park

scholarly journals Mycelial biomass cultivation of Lentinus crinitus

2020 ◽  
Vol 36 (6) ◽  
Author(s):  
Itaruã Machri Colla ◽  
Olavo Bilac Quaresma de Oliveira Filho ◽  
Janyeli Dorini Silva de Freitas ◽  
Míria Benetati Delgado Bertéli ◽  
Giani Andrea Linde ◽  
...  
Keyword(s):  
Soybean Meal ◽  
Nitrogen Concentration ◽  
Fungal Growth ◽  
Malt Extract ◽  
Biomass Growth ◽  
Mycelial Biomass ◽  
Protein Nitrogen ◽  
Temperature Growth ◽  
Nitrogen Concentrations ◽  
High Concentrations

Lentinus crinitus is a medicinal basidiomycete, little studied regarding the basic cultivation conditions, which is used in bioremediation and consumed by native Indians from the Brazilian Amazon. Also, it produces a fungal secondary metabolite panepoxydone that has been described as an essential regulator of the inflammatory and immune response. This study aimed to evaluate basic conditions of temperature, pH, and nitrogen concentration and source in the cultivation of L. crinitus mycelial biomass. In order to evaluate fungal growth temperature, 2% malt extract agar (MEA) medium, pH 5.5, was utilized from 19 to 40 °C. For pH, MEA had pH adjusted from 2 to 11 and cultivated at 28 °C. Urea or soybean meal was added to MEA to obtain final concentration from 0.5 and 16 g/L of nitrogen, pH of 5.5, cultivated at 28 °C. The best temperature growth varies from 31 to 34 ºC and the optimal one is 32.7º C, and the best pH ranges from 4.5 to 6.5 and the optimal one is 6.1. Protein or non-protein nitrogen concentration is inversely proportional to the mycelial biomass growth. Nitrogen concentrations of 2.0 g/L soybean meal and urea inhibit mycelial biomass growth in 11% and 12%, respectively, but high concentrations of 16.0 g/L nitrogen inhibit the growth in 46% and 95%, respectively. The fungus is robust and grows under extreme conditions of temperature and pH, but smaller adaptation with increasing nitrogen concentrations in the cultivation medium, mainly non-protein nitrogen.

scholarly journals DETERMINATION OF OPTIMUM CULTURE CONDITIONS FOR MYCELIAL GROWTH OF Macrolepiota procera MUSHROOM

2020 ◽  
Vol 19 (1) ◽  
pp. 11-20
Author(s):  
Aysun Pekşen ◽  
Beyhan Kibar
Keyword(s):  
Yeast Extract ◽  
Mycelial Growth ◽  
Nitrogen Sources ◽  
Culture Conditions ◽  
Malt Extract ◽  
The Public ◽  
Carbon And Nitrogen ◽  
Ph Values ◽  
Incubation Temperatures ◽  
Macrolepiota Procera

Macrolepiota procera, commonly called the Parasol Mushroom, is a delicious mushroom collected from the nature and commonly consumed by the public in many regions of Turkey. This study was conducted to determine the optimum culture conditions (pH, temperature, carbon and nitrogen sources) for mycelial growth of M. procera. Three pH values (pH 5.0, 5.5 and 6.0), four incubation temperatures (15, 20, 25 and 30°C), seven carbon (C) sources (dextrose, glucose, lactose, maltose, mannitol, sucrose and xylose) and six nitrogen (N) sources ((NH4)2HPO4, NH4NO3 and Ca(NO3)2, malt extract, peptone and yeast extract) were investigated. In the second step of the study, the effect of seven pH values (4.0, 4.5, 5.0, 5.5, 6.0, 6.5 and 7.0) on the mycelial colony diameter was examined at 20 and 25°C since these temperatures gave the best mycelial growth in the previously conducted temperature experiment. The best mycelial growth was determined at pH 6.0. The optimum temperature for mycelial growth of M. procera was found as 25°C. The use of glucose as carbon source and yeast extract and peptone as nitrogen source in the culture medium gave the best results for mycelial growth. Determining of optimum culture conditions for mycelial growth of M. procera will provide important contributions to the fortcoming studies on it’s commercially cultivation in Turkey.

scholarly journals A comparison of ten USEPA approved total coliform/E. coli tests

2007 ◽  
Vol 5 (2) ◽  
pp. 267-282 ◽  
Author(s):  
Jeremy Olstadt ◽  
James Jay Schauer ◽  
Jon Standridge ◽  
Sharon Kluender
Keyword(s):  
Environmental Protection Agency ◽  
False Positive ◽  
The United States ◽  
Total Coliform ◽  
Test System ◽  
E Coli ◽  
Test Systems ◽  
High Concentrations ◽  
Different Strains ◽  
Positive Results

Since 2002, the United States Environmental Protection Agency (USEPA) has approved ten enzyme-based total coliform and E. coli detection tests for examination of drinking water. These tests include: Colilert®, Colilert-18®, Colisure®, m-Coli Blue 24®, Readycult® Coliforms 100, Chromocult®, Coliscan®, E*Colite®, Colitag™ and MI Agar. The utility of the enzyme based test systems is based on both the ability of the test to detect the target organisms at low levels and the ability of the test system to suppress the growth of non-target organisms that might result in false positive results. Differences in the ability of some of these methods to detect total coliform and E. coli, as well as suppress Aeromonas spp., a common cause of “false positive” results, have been observed. As a result, this study was undertaken to elucidate the strengths and weaknesses of each method. Water samples were collected from three geographically and chemically diverse groundwaters in Wisconsin. One-hundred milliliter aliquots were individually spiked with both low concentrations (one to ten organisms) and high concentrations (fifty to one-hundred) of each of five different total coliform organisms (Serratia, Citrobacter, Enterobacter, E. coli, & Klebsiella). These spiked samples were used to test the capability of ten enzyme-based test systems to both detect and enumerate the spiked organisms. In addition, 100 ml samples were independently spiked with two different strains of Aeromonas spp. at six different levels, to assess the ability of each enzyme-based test to suppress Aeromonas spp. Analysis of the data indicated that wide variability exists among USEPA approved tests to detect and quantify total coliforms, as well as suppress Aeromonas spp.

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