scholarly journals Whole-genome sequencing analysis reveals the population history of Mus musculus in Madagascar

2021 ◽  
Author(s):  
Kazumichi Fujiwara ◽  
Marie C Ranorosoa ◽  
Satoshi D Ohdachi ◽  
Satoru Arai ◽  
Yuki Sakuma ◽  
...  
Keyword(s):  
House Mouse ◽  
Mus Musculus ◽  
Population History ◽  
House Mice ◽  
Whole Genome ◽  
Sequencing Analysis ◽  
Mouse Population ◽  
Wide Range ◽  
History Of ◽  
Ancient Hybridization

AbstractIn Madagascar, the house mouse (Mus musculus) is thought to have colonized along with humans and is now one of the most successfully colonized rodents on the island. In this study, we determined the whole-genome sequences of the Madagascar house mouse captured from the wild. We examined the evolutionary history of its population regarding the mitochondrial and autosomal genomes. We confirmed that in the mitochondrial genomes of Madagascar house mice, a monophyletic clade forms a basal origin within the species. An analysis of autosomal genomic sequences indicates that the Madagascar house mouse population is genetically a member of M. m. castaneus (CAS). It also contains genetic elements of M. m. domesticus (DOM) resulting from ancient hybridization. The signature of a strong population bottleneck 1000–3000 years ago was observed in the mitochondrial and autosomal genomic data. We also show that the divergence of the Madagascar population from the CAS population occurred approximately 50,000–99,000 years ago. Madagascar house mice show strong genetic affinity to many CAS samples across a wide range of Indian Ocean coastal regions. However, our results suggest that they would not have originated directly from the Indonesian islands, where Austronesian-speaking people in Madagascar originated. Because the ancient hybridization signature with DOM did not appear in the Indonesian and other CAS samples, we propose that Madagascar house mice were not directly brought by Austronesian-speaking people but came from somewhere around the Middle East or South Asia soon after the colonization of initial farmers.

scholarly journals Insights into mammalian biology from the wild house mouse Mus musculus

eLife ◽  
2015 ◽  
Vol 4 ◽  
Author(s):  
Megan Phifer-Rixey ◽  
Michael W Nachman
Keyword(s):  
House Mouse ◽  
Mus Musculus ◽  
Genetic Model ◽  
Inbred Strains ◽  
Mendelian Inheritance ◽  
Model Organisms ◽  
House Mice ◽  
Small Subset ◽  
Wild Mice ◽  
Wide Range

The house mouse, Mus musculus, was established in the early 1900s as one of the first genetic model organisms owing to its short generation time, comparatively large litters, ease of husbandry, and visible phenotypic variants. For these reasons and because they are mammals, house mice are well suited to serve as models for human phenotypes and disease. House mice in the wild consist of at least three distinct subspecies and harbor extensive genetic and phenotypic variation both within and between these subspecies. Wild mice have been used to study a wide range of biological processes, including immunity, cancer, male sterility, adaptive evolution, and non-Mendelian inheritance. Despite the extensive variation that exists among wild mice, classical laboratory strains are derived from a limited set of founders and thus contain only a small subset of this variation. Continued efforts to study wild house mice and to create new inbred strains from wild populations have the potential to strengthen house mice as a model system.

scholarly journals Mus musculus populations in Western Australia lack VKORC1 mutations conferring resistance to first generation anticoagulant rodenticides: Implications for conservation and biosecurity

2020 ◽  
Author(s):  
Bridget J.M.L. Duncan ◽  
Annette Koenders ◽  
Quinton Burnham ◽  
Michael T. Lohr
Keyword(s):  
House Mouse ◽  
Western Australia ◽  
Mus Musculus ◽  
House Mice ◽  
Target Species ◽  
Mouse Population ◽  
Anticoagulant Rodenticides ◽  
D Loop ◽  
Rodent Populations ◽  
Vkorc1 Gene

AbstractBackgroundHumans routinely attempt to manage pest rodent populations with anticoagulant rodenticides (ARs). We require information on resistance to ARs within rodent populations to have effective eradication programs that minimise exposure in non-target species. Mutations to the VKORC1 gene have been shown to confer resistance in rodents with high proportions of resistance in mice found in all European populations tested. We screened mutations in Mus musculus within Western Australia, by sampling populations from the capital city (Perth) and a remote island (Browse Island). These are the first Australian mouse populations screened for resistance using this method. Additionally, the mitochondrial D-loop of house mice was sequenced to explore population genetic structure, identify the origin of Western Australian mice, and to elucidate whether resistance was linked to certain haplotypes.ResultsNo resistance-related VKORC1 mutations were detected in either house mouse population. A genetic introgression in the intronic sequence of the VKORC1 gene of Browse Island house mouse was detected which is thought to have originated through hybridisation with the Algerian mouse (Mus spretus). Analysis of the mitochondrial D-loop reported two haplotypes in the house mouse population of Perth, and two haplotypes in the population of Browse Island.ConclusionsBoth house mouse populations exhibited no genetic resistance to ARs, in spite of free use of ARs in Western Australia. Therefore weaker anticoagulant rodenticides can be employed in pest control and eradication attempts, which will result in reduced negative impacts on non-target species. Biosecurity measures must be in place to avoid introduction of resistant house mice, and new house mouse subspecies to Western Australia.

scholarly journals Clonal Lineages of Erysipelothrix rhusiopathiae Responsible for Acute Swine Erysipelas in Japan Identified by Using Genome-Wide Single-Nucleotide Polymorphism Analysis

2017 ◽  
Vol 83 (11) ◽  
Author(s):  
Yohsuke Ogawa ◽  
Kazumasa Shiraiwa ◽  
Yoshitoshi Ogura ◽  
Tadasuke Ooka ◽  
Sayaka Nishikawa ◽  
...  
Keyword(s):  
Polymorphism Analysis ◽  
Whole Genome ◽  
Sequencing Analysis ◽  
Erysipelothrix Rhusiopathiae ◽  
Single Nucleotide ◽  
Content Type ◽  
Genome Wide ◽  
Wide Range ◽  
Swine Erysipelas ◽  
Lineage Iv

ABSTRACTErysipelothrix rhusiopathiaecauses swine erysipelas, an important infectious disease in the swine industry. In Japan, the incidence of acute swine erysipelas due toE. rhusiopathiaeserovar 1a has recently increased markedly. To study the genetic relatedness of the strains from the recent cases, we analyzed 34E. rhusiopathiaeserovar 1a swine isolates collected between 1990 and 2011 and further investigated the possible association of the live Koganei 65-0.15 vaccine strain (serovar 1a) with the increase in cases. Pulsed-field gel electrophoresis analysis revealed no marked variation among the isolates; however, sequencing analysis of a hypervariable region in the surface-protective antigen A gene (spaA) revealed that the strains isolated after 2007 exhibited the samespaAgenotype and could be differentiated from older strains. Phylogenetic analysis based on genome-wide single-nucleotide polymorphisms (SNPs) revealed that the Japanese strains examined were closely related, showing a relatively small number of SNPs among them. The strains were classified into four major lineages, with Koganei 65-0.15 (lineage III) being phylogenetically separated from the other three lineages. The strains isolated after 2007 and the two older strains constituted one major lineage (lineage IV) with a specificspaAgenotype (M203/I257-SpaA), while the recent isolates were further divided into two geographic groups. The remaining older isolates belonged to either lineage I, with the I203/L257-SpaA type, or lineage II, with the I203/I257-SpaA type. These results indicate that the recent increased incidence of acute swine erysipelas in Japan is associated with two sublineages of lineage IV, which have independently evolved in two different geographic regions.IMPORTANCEUsing large-scale whole-genome sequence data fromErysipelothrix rhusiopathiaeisolates from a wide range of hosts and geographic origins, a recent study clarified the existence of three distinct clades (clades 1, 2, and 3) that are found across multiple continents and host species, representing both livestock and wildlife, and an “intermediate” clade between clade 2 and the dominant clade 3 within the species. In this study, we found that theE. rhusiopathiaeJapanese strains examined exhibited remarkably low levels of genetic diversity and confirmed that all of the Japanese and Chinese swine isolates examined in this study belong to clonal lineages within the intermediate clade. We report thatspaAgenotyping ofE. rhusiopathiaestrains is a practical alternative to whole-genome sequencing analysis of theE. rhusiopathiaeisolates from eastern Asian countries.

scholarly journals Host subspecific viral strains in European house mice: Murine cytomegalovirus in the Eastern (Mus musculus musculus) and Western house mouse (Mus musculus domesticus)

Virology ◽  
2018 ◽  
Vol 521 ◽  
pp. 92-98 ◽  
Author(s):  
Dagmar Čížková ◽  
Stuart J.E. Baird ◽  
Jana Těšíková ◽  
Sebastian Voigt ◽  
Ďureje Ľudovít ◽  
...  
Keyword(s):  
House Mouse ◽  
Mus Musculus ◽  
Murine Cytomegalovirus ◽  
House Mice ◽  
Mus Musculus Domesticus ◽  
Mus Musculus Musculus

scholarly journals Diverse tumour susceptibility in Collaborative Cross mice: identification of a new mouse model for human gastric tumourigenesis

Gut ◽  
2019 ◽  
Vol 68 (11) ◽  
pp. 1942-1952 ◽  
Author(s):  
Pin Wang ◽  
Yunshan Wang ◽  
Sasha A Langley ◽  
Yan-Xia Zhou ◽  
Kuang-Yu Jen ◽  
...  
Keyword(s):  
Inflammatory Response ◽  
Mouse Model ◽  
Rna Sequencing ◽  
Genetic Association ◽  
Association Analysis ◽  
Collaborative Cross ◽  
Sequencing Analysis ◽  
Genetic Association Analysis ◽  
Mouse Population ◽  
Wide Range

ObjectiveThe Collaborative Cross (CC) is a mouse population model with diverse and reproducible genetic backgrounds used to identify novel disease models and genes that contribute to human disease. Since spontaneous tumour susceptibility in CC mice remains unexplored, we assessed tumour incidence and spectrum.DesignWe monitored 293 mice from 18 CC strains for tumour development. Genetic association analysis and RNA sequencing were used to identify susceptibility loci and candidate genes. We analysed genomes of patients with gastric cancer to evaluate the relevance of genes identified in the CC mouse model and measured the expression levels of ISG15 by immunohistochemical staining using a gastric adenocarcinoma tissue microarray. Association of gene expression with overall survival (OS) was assessed by Kaplan-Meier analysis.ResultsCC mice displayed a wide range in the incidence and types of spontaneous tumours. More than 40% of CC036 mice developed gastric tumours within 1 year. Genetic association analysis identified Nfκb1 as a candidate susceptibility gene, while RNA sequencing analysis of non-tumour gastric tissues from CC036 mice showed significantly higher expression of inflammatory response genes. In human gastric cancers, the majority of human orthologues of the 166 mouse genes were preferentially altered by amplification or deletion and were significantly associated with OS. Higher expression of the CC036 inflammatory response gene signature is associated with poor OS. Finally, ISG15 protein is elevated in gastric adenocarcinomas and correlated with shortened patient OS.ConclusionsCC strains exhibit tremendous variation in tumour susceptibility, and we present CC036 as a spontaneous laboratory mouse model for studying human gastric tumourigenesis.

scholarly journals Field trials of calciferol combined with warfarin against wild house-mice (Mus musculus L.)

1974 ◽  
Vol 73 (3) ◽  
pp. 353-360 ◽  
Author(s):  
F. P. Rowe ◽  
F. J. Smith ◽  
T. Swinney
Keyword(s):  
Vitamin D ◽  
House Mouse ◽  
Mus Musculus ◽  
Corn Oil ◽  
Field Trials ◽  
Post Treatment ◽  
House Mice ◽  
Effective Combination ◽  
Wild House Mice ◽  
Farm Buildings

SummaryA combination of calciferol (vitamin D2) and warfarin, each at 0·025% in medium oatmeal bait, failed to control six of seven house-mouse (Mus musculus L.) populations infesting urban and farm buildings. In three further treatments with both calciferol and warfarin at 0·05 % in dehusked canary seed bait plus 5% corn oil, mortality, estimated from the consumption of pre- and post-treatment census bait, ranged between 94·2 and 97·4%. Finally, among sixteen treatments done with calciferol at 0·1% and warfarin at 0·025% in various cereal baits, the best results (97·0–100%) were obtained in six treatments where the bait-base was whole canary seed; this was so whether the poison bait was applied directly or after a 3-day pre-baiting period. It is concluded that calciferol at 0·1 % plus warfarin at 0·025 % is an effective combination against house-mice, especially when used with whole canary seed. The role played by warfarin in the poison mixture needs to be investigated further.

scholarly journals The toxicity and acceptability of the sodium salt of pindone, an anti-coagulant rodenticide, to the house-mouse (Mus musculus L.)

1961 ◽  
Vol 59 (3) ◽  
pp. 335-341 ◽  
Author(s):  
F. P. Rowe
Keyword(s):  
House Mouse ◽  
Sodium Salt ◽  
Mus Musculus ◽  
House Mice ◽  
Wild House Mice ◽  
The Difference

1. A 0·005% solution of the sodium salt of pindone was found to kill wild house-mice (Mus musculus) in 4–6 days. Mice offered a choice between this solution and water drank more water, but the difference in consumption was not statistically significant.2. The addition of 1% sugar did not appreciably alter the palatability of a 0·005% solution, but a solution of the anti-coagulant containing 10% sugar was more readily accepted than water. This preference was maintained with solution 4 months old.

scholarly journals Field trials of the rodenticide 5-p-chlorophenyl silatrane against wild house mice (Mus musculus L.)

1974 ◽  
Vol 73 (1) ◽  
pp. 49-52 ◽  
Author(s):  
F. P. Rowe ◽  
T. Swinney ◽  
A. Bradfield
Keyword(s):  
House Mouse ◽  
Mus Musculus ◽  
Corn Oil ◽  
Treatment Success ◽  
Field Trials ◽  
Zinc Phosphide ◽  
House Mice ◽  
Before And After ◽  
Wild House Mice

SUMMARYThe performance of the rodenticide 5-p-chlorophenyl silatrane at 0.5% in a wholemeal flour/pinhead oatmeal/corn oil bait was compared with that of zinc phosphide at 3% in the same base in poison treatments carried out against urban infestations of the house mouse (Mus musculus L.). Each poison treatment was conducted for 1 day and after 3 days' pre-baiting. The success of the treatments was assessed from census baitings conducted before and after treatment. Treatment success varied considerably with both poisons used but in general 5-p-chlorophenyl silatrane proved to be at least as effective as zinc phosphide, a commonly used acute rodenticide for the control of mice.

scholarly journals Trials of the anticoagulant rodenticide WBA 8119 against confined colonies of warfarin-resistant house mice (Mus musculus L.)

1976 ◽  
Vol 77 (3) ◽  
pp. 427-431 ◽  
Author(s):  
F. P. Rowe ◽  
A. Bradfield
Keyword(s):  
House Mouse ◽  
Mus Musculus ◽  
Toxicity Tests ◽  
House Mice ◽  
Anticoagulant Rodenticide ◽  
Laboratory Findings ◽  
Anticoagulant Rodenticides ◽  
Choice Feeding ◽  
Better Than ◽  
Family Groups

SUMMARYThe efficacy of the newly developed anticoagulant rodenticide WBA 8119 was evaluated against the house mouse (Mus musculus L.) using individual and family groups of warfarin-resistant animals. WBA 8119 at 0·002 %, O % and 0.01 % in pinhead oatmeal bait gave complete kills of mice in ‘no-choice’ feeding tests carried out in cages and small pens. In replicated 21-day treatments on families of mice confined in larger pens and conditioned to feeding on plain foods, the overall mortalities obtained using the three formulated poison baits were 71/72, 62/63 and 57/57 respectively.The results of the WBA 8119 toxicity tests are considered in relation to previous findings on other anticoagulant rodenticides, particularly difenacoum. In equivalent tests, WBA 8119 performed better than difenacoum. The data thus support the laboratory findings that WBA 8119 is the most active anticoagulant so far tested for the control of warfarin-resistant house mice.

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