scholarly journals Multiple spillovers and onward transmission of SARS-Cov-2 in free-living and captive White-tailed deer (Odocoileus virginianus)

2021 ◽  
Author(s):  
Suresh V Kuchipudi ◽  
Meera Surendran-Nair ◽  
Rachel M Ruden ◽  
Michele Yon ◽  
Ruth H Nissly ◽  
...  
Keyword(s):  
Lymph Node ◽  
North America ◽  
Odocoileus Virginianus ◽  
One Health ◽  
Animal Species ◽  
Whole Genome ◽  
Rt Pcr ◽  
Retropharyngeal Lymph Node ◽  
Free Living

Many animal species are susceptible to SARS-CoV-2 and could potentially act as reservoirs, yet transmission in non-human free-living animals has not been documented. White-tailed deer (Odocoileus virginianus), the predominant cervid in North America, are susceptible to SARS-CoV-2 infection, and experimentally infected fawns transmit the virus to other captive deer. To test the hypothesis that SARS-CoV-2 may be circulating in deer, we evaluated 283 retropharyngeal lymph node (RPLN) samples collected from 151 free-living and 132 captive deer in Iowa from April 2020 through December of 2020 for the presence of SARS-CoV-2 RNA. Ninety-four of the 283 deer (33.2%; 95% CI: 28, 38.9) samples were positive for SARS-CoV-2 RNA as assessed by RT-PCR. Notably, between Nov 23, 2020, and January 10, 2021, 80 of 97 (82.5%; 95% CI 73.7, 88.8) RPLN samples had detectable SARS-CoV-2 RNA by RT-PCR. Whole genome sequencing of the 94 positive RPLN samples identified 12 SARS-CoV-2 lineages, with B.1.2 (n = 51; 54.5%), and B.1.311 (n = 19; 20%) accounting for ~75% of all samples. The geographic distribution and nesting of clusters of deer and human lineages strongly suggest multiple zooanthroponotic spillover events and deer-to-deer transmission. The discovery of sylvatic and enzootic SARS-CoV-2 transmission in deer has important implications for the ecology and long-term persistence, as well as the potential for spillover to other animals and spillback into humans. These findings highlight an urgent need for a robust and proactive One Health approach to obtaining a better understanding of the ecology and evolution of SARS-CoV-2.

scholarly journals Porcine Circovirus 3a Field Strains in Free-Living Wild Boars in Paraná State, Brazil

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1634
Author(s):  
Tatiana Carolina Gomes Dutra de Souza ◽  
Danielle Gava ◽  
Rejane Schaefer ◽  
Raquel Arruda Leme ◽  
Gisele da Silva Porto ◽  
...  
Keyword(s):  
Animal Species ◽  
Porcine Circovirus ◽  
Whole Genome ◽  
Living Animal ◽  
Pcr Assay ◽  
Serum Samples ◽  
Viral Dna ◽  
Free Living ◽  
Wild Boars ◽  
The World

Porcine circovirus 3 (PCV-3) was identified in domestic pigs worldwide. Although PCV-3 has also been detected in wild boars, information regarding its circulation in this free-living animal species is scarce. To investigate PCV-3 occurrence in free-living wild boars in Brazil, 70 serum samples collected between January 2017 and June 2019 in Paraná state, Brazil were analyzed by PCR assay. Amplicons measuring 330 bp in length were amplified in seven (10.0%) of the serum samples and confirmed to be PCV3-specific by nucleotide (nt) sequencing. As the amplified products from the serum samples yielded only intermediate levels of viral DNA, lung samples from the seven PCR-positive wild boars were also evaluated by PCR. Of these samples, five lung samples were positive and provided high levels of viral DNA. The three lung samples that presented the highest levels of viral DNA were selected for amplification and sequencing of the whole PCV-3 genome. The three full-length sequences obtained were grouped in PCV-3 clade “a”, and the sequences exhibited 100% nucleotide similarity among them. The PCV-3 field strains of this study showed nucleotide and amino acid similarities of 98.5–99.8% and 98.8–100%, respectively, with whole-genome PCV-3 sequences from around the world.

scholarly journals Screening for West Nile virus infections of susceptible animal species in Austria

2003 ◽  
Vol 131 (2) ◽  
pp. 1023-1027 ◽  
Author(s):  
H. WEISSENBÖCK ◽  
Z. HUBÁLEK ◽  
J. HALOUZKA ◽  
A. PICHLMAIR ◽  
A. MADERNER ◽  
...  
Keyword(s):  
West Nile Virus ◽  
Animal Species ◽  
Eastern European ◽  
Virus Infections ◽  
Rt Pcr ◽  
West Nile ◽  
Free Living ◽  
Susceptible Animal ◽  
Eastern European Countries

Avian mortality and encephalomyelitis in equines are considered good indicators for West Nile virus (WNV) activity. We retrospectively tested 385 horse sera for WNV antibodies and looked for WNV nucleic acid and/or WNV antigen in paraffin embedded tissues from 12 horses with aetiologically unresolved encephalomyelitis and 102 free-living birds of different species which had been found dead. With the exception of four horses originating from eastern European countries investigated on the occasion of transit through Austria, all horse sera were negative. Nested RT-PCR of the horse tissues yielded no amplification of WNV-RNA. Also, all bird samples, examined by immunohistochemistry, in situ hybridization and nested RT-PCR were negative for WNV. These results indicate that currently WNV cannot be considered a significant pathogen in Austria.

Could marten become the spotted owl of eastern Canada?

1991 ◽  
Vol 67 (2) ◽  
pp. 136-140 ◽  
Author(s):  
Ian D. Thompson
Keyword(s):  
North America ◽  
Land Use Planning ◽  
Prince Edward Island ◽  
Animal Species ◽  
Resource Planning ◽  
Eastern Canada ◽  
Spotted Owl ◽  
Old Growth Forest ◽  
Management Plans

Conservation of spotted owl habitat in western North America illustrates the difficult decisions that must be taken and the conflicts that can arise in land-use planning. In eastern North America, spotted owls are absent but marten, an animal species which prefers old-growth forest, has become rare in some areas as a result of habitat loss. The marten is a threatened species in Newfoundland, exists in small numbers in Nova Scotia, and has been extirpated in Prince Edward Island. Lack of long-term integrated forest resource planning, short rotations, and silvicultural practices that produce sub-optimal habitat may eliminate the species in Atlantic Canada. Two cases are discussed from Newfoundland and New Brunswick where unbalanced forest age structures suggest a bleak future for the marten. Other larger jurisdictions in Canada should closely examine their forest land management plans in view of the Atlantic experience.

scholarly journals 189. Potential Tiger-to-Human Transmission of SARS-CoV-2 at a Tennessee Zoo: A One Health Approach to Outbreak Investigation

2021 ◽  
Vol 8 (Supplement_1) ◽  
pp. S114-S115
Author(s):  
Heather N Grome ◽  
Becky Meyer ◽  
Erin Read ◽  
Martha Buchanan ◽  
Andrew C Cushing ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Single Nucleotide Polymorphisms ◽  
Environmental Assessment ◽  
One Health ◽  
Observation Point ◽  
Outbreak Investigation ◽  
Whole Genome ◽  
Nucleotide Polymorphisms ◽  
Rt Pcr ◽  
Single Nucleotide

Abstract Background Human-to-feline and airborne transmission among cats of Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) has been described, though documented feline-to-human transmission has not been reported. In October 2020, all 3 Malayan tigers at a Tennessee AZA accredited zoo were diagnosed with symptomatic SARS-CoV-2 infection. We investigated to determine source and prevent further transmission. Methods Tiger nasal swab specimens were tested at the National Veterinary Services Laboratories (NVSL). An environmental assessment at the zoo was completed. We interviewed 18 staff who interacted with the tigers during the 2 weeks before animal symptom onset. Confirmed human cases were defined as persons testing positive for SARS-CoV-2 by RT-PCR during September 28–October 29, with tiger interaction during their 14-day incubation period. Interviewed staff had repeat SARS-CoV-2 RT-PCR and serum IgG testing on October 29. Tigers and staff testing positive had specimens sent to CDC for genomic sequencing. Tiger sequences were compared phylogenetically with 30 geographically associated human cases collected within 2 weeks of the outbreak and > 200 background sequences from TN. Results NVSL confirmed SARS-CoV-2 infection in all 3 tigers. Environmental assessment identified fencing between humans and animals allowing airflow and an open outdoor exhibit observation point above the habitat. Confirmed cases were identified in a tiger keeper and veterinary assistant; both developed symptoms after exposure to symptomatic tigers and one sample was genotyped. Staff did not report known contact with ill visitors. All staff were negative for SARS-CoV-2 IgG. The tigers and most temporally and geographically associated cases had genetic sequences in clade 20G and B.1.2. Tiger sequences were 3-6 single nucleotide polymorphisms different from the positive tiger keeper (Figure). Figure. Whole-genome phylogenetic analysis. Whole-genome phylogenetic analysis from a portion of clade 20G showing divergence estimates from SARS-CoV-2 Wuhan-Hu-1 reference genome with sequences from humans living in Tennessee and Malayan tigers sampled during the outbreak investigation in October 2020. Sequence analysis showed 3-6 single nucleotide polymorphisms (SNPs) differences between one human tiger keeper and all three tiger sequences. Differences are indicated by one-step edges (lines) between colored dots (individual SARS-CoV-2 sequenced infections). Numbers indicate unique sequences. Note not all analyzed sequences are shown in this figure. Conclusion Using a One Health approach, we concluded the index tiger was likely infected via transmission from an ill visitor at an exhibit observation point or unidentified asymptomatic staff. Infection spread to the other 2 tigers and tiger-to-human transmission to 2 staff is possible thereafter. The zoo was advised on infection control practices for humans and animals, and no additional cases were identified. Disclosures William Schaffner, MD, VBI Vaccines (Consultant)

scholarly journals Comparison of Chronic Wasting Disease Detection Methods and Procedures: implications for free-ranging white-tailed deer (Odocoileus virginianus) surveillance and management

2021 ◽  
Author(s):  
Marc D Schwabenlander ◽  
Gage R Rowden ◽  
Manci Li ◽  
Kelsie LaSharr ◽  
Erik C Hildebrand ◽  
...  
Keyword(s):  
Lymph Node ◽  
North America ◽  
Lymph Nodes ◽  
Odocoileus Virginianus ◽  
Chronic Wasting Disease ◽  
Enzyme Linked Immunosorbent Assay ◽  
Lymphoid Tissues ◽  
Sample Type ◽  
Wasting Disease ◽  
Free Ranging

Throughout North America, chronic wasting disease (CWD) has emerged as perhaps the greatest threat to wild cervid populations, including white-tailed deer (Odocoileus virginianus). White-tailed deer are the most sought after big game species across North America with populations of various subspecies in nearly all Canadian provinces, the contiguous USA, and Mexico. Documented CWD cases have dramatically increased across the white-tailed deer range since the mid-1990s, including in Minnesota. CWD surveillance in free-ranging white-tailed deer and other cervid populations mainly depends upon immunodetection methods (e.g., immunohistochemistry [IHC] and enzyme-linked immunosorbent assay [ELISA]) on medial retropharyngeal lymph nodes and obex. More recent technologies centered on prion protein amplification methods of detection have shown promise as more sensitive and rapid CWD diagnostic tools. Here, we used blinded samples to test the efficacy of real time quaking-induced conversion (RT-QuIC) in comparison to ELISA and IHC for screening tissues, blood, and feces collected in 2019 from white-tailed deer in southeastern Minnesota, where CWD has been routinely detected since 2016. Our results support previous findings that RT-QuIC is a more sensitive tool for CWD detection than current antibody-based methods. Additionally, a CWD testing protocol that includes multiple lymphoid tissues (medial retropharyngeal lymph node, parotid lymph node, and palatine tonsil) per animal may effectively identify a greater number of CWD detections in a white-tailed deer population than a single sample type (i.e., medial retropharyngeal lymph nodes). These results reveal that the variability of CWD pathogenesis, sampling protocol, and testing platform must be considered for the effective detection and management of CWD throughout North America.

427: Long Term Results of Laparoscopic Retroperitoneal Lymph Node Dissection in Clinical Stage I NSGCT

2005 ◽  
Vol 173 (4S) ◽  
pp. 116-117
Author(s):  
Hannes Steiner ◽  
Reinhard Peschel ◽  
Tilko Müller ◽  
Christian Gozzi ◽  
Georg C. Bartsch ◽  
...  
Keyword(s):  
Lymph Node ◽  
Lymph Node Dissection ◽  
Clinical Stage ◽  
Stage I ◽  
Long Term Results ◽  
Retroperitoneal Lymph Node Dissection ◽  
Node Dissection ◽  
Retroperitoneal Lymph Node

scholarly journals Detection of SARS-CoV-2 from patient fecal samples by whole genome sequencing

Gut Pathogens ◽  
2021 ◽  
Vol 13 (1) ◽  
Author(s):  
Andreas Papoutsis ◽  
Thomas Borody ◽  
Siba Dolai ◽  
Jordan Daniels ◽  
Skylar Steinberg ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Nasopharyngeal Swab ◽  
Whole Genome ◽  
Rt Pcr ◽  
Whole Genome Analysis ◽  
Fecal Samples ◽  
Oral Transmission ◽  
Study Participants ◽  
Next Generation Sequencing Ngs ◽  
Generation Sequencing

Abstract Background SARS-CoV-2 has been detected not only in respiratory secretions, but also in stool collections. Here were sought to identify SARS-CoV-2 by enrichment next-generation sequencing (NGS) from fecal samples, and to utilize whole genome analysis to characterize SARS-CoV-2 mutational variations in COVID-19 patients. Results Study participants underwent testing for SARS-CoV-2 from fecal samples by whole genome enrichment NGS (n = 14), and RT-PCR nasopharyngeal swab analysis (n = 12). The concordance of SARS-CoV-2 detection by enrichment NGS from stools with RT-PCR nasopharyngeal analysis was 100%. Unique variants were identified in four patients, with a total of 33 different mutations among those in which SARS-CoV-2 was detected by whole genome enrichment NGS. Conclusion These results highlight the potential viability of SARS-CoV-2 in feces, its ongoing mutational accumulation, and its possible role in fecal–oral transmission. This study also elucidates the advantages of SARS-CoV-2 enrichment NGS, which may be a key methodology to document complete viral eradication. Trial registration ClinicalTrials.gov, NCT04359836, Registered 24 April 2020, https://clinicaltrials.gov/ct2/show/NCT04359836?term=NCT04359836&draw=2&rank=1).

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