scholarly journals Biosorption potential and molecular characterization of metal resistant autochthonous microbes from tannery solid waste

2021 ◽  
Author(s):  
Hajira Younas ◽  
Aisha Nazir ◽  
Zakia Latif ◽  
Janice E Thies ◽  
Muhammad Shafiq ◽  
...  
Keyword(s):  
Solid Waste ◽  
Its Region ◽  
Metal Resistance ◽  
Molecular Techniques ◽  
Bacterial Strains ◽  
Fungal Strains ◽  
Resistant Strains ◽  
Tannery Solid Waste ◽  
Clean Metal

This study encompasses isolation and screening of heavy metal-resistant fungal and bacterial strains from tannery solid waste (TSW). Twelve fungal strains and twenty-five bacterial strains were isolated from TSW. The growth of fungal strains was observed against different heavy metals ranging from 10 mg L -1 to 1050 mg L -1 and the growth of bacteria was observed in metal concentrations ranging from 10 mg L -1 to 1200 mg L -1 . Five multi-metal resistant fungal isolates belonging to the genus Trichoderma and ten bacterial isolates belonging to the genus Bacillus showed good metal resistance and biosorption potential. They were identified through molecular techniques, fungi based on ITS region ribotyping, and bacteria based on 16S rRNA ribotyping. The fungal strains were characterized as T. hamatum (TSWF-06), T. harzianum (TSWF-11), T. lixii (TSWF-02) and T. pseudokoningii (TSWF-03, TSWF-10). The bacterial strains were characterized as Bacillus xiamenensis (TSW-02), B. velezensis (TSW-05), B. piscis (TSW-06), B. safensis (TSW-10), B. subtilis (TSW-14, TSW-15, TSW-17) B. licheniformis (TSW-19), B. cereus (TSW-20) and B. thuringiensis (TSW-22). The fungal strains namely, T. pseudokoningii (TSWF-03) and T. harzianum proved to be two multi-metal resistant strains with good biosorption efficiency. Unlike fungi, bacterial strains showed metal specific resistance. The strains Bacillus xiamenensis , B. subtilis (TSW-14) and B. subtilis (TSW-15) showed good biosorption efficiency against Cr, B. safensis against Cu, B. piscis and B. subtilis (TSW-17) against Pb and B. licheniformis and B. thuringiensis against Zn. The autochthonous fungal and bacterial strains can therefore be employed to clean metal contaminated environments.

scholarly journals Synthesis and Characterization of Substituted Bisbenzoxazine as Potent Antimicrobial Agent

2014 ◽  
Vol 40 ◽  
pp. 73-80 ◽  
Author(s):  
Kishor R. Lalcheta ◽  
Bhvin B. Dhaduk ◽  
Jaymin P. Mendapara
Keyword(s):  
Antimicrobial Activity ◽  
Antimicrobial Agent ◽  
Synthesis And Characterization ◽  
Bacterial Strains ◽  
Fungal Strains

Bisbenoxazines derivatives were synthesized by refluxing substituted bisphenol-C, substituted aniline and formaldehyde in presence of 1,4-dioxane with good yields. All the synthesized compounds were characterized by mass, NMR and IR and also evaluated for antimicrobial activity against four different bacterial and two fungal strains. The compounds 1c, 1h, 1j and 1l has found comparatively good active against all the bacterial strains.

scholarly journals Synthesis and Characterization of Fully Substituted Pyrimidines by Using Ketene Dithioacetal as Potent Antimicrobial Agent

2013 ◽  
Vol 24 ◽  
pp. 134-141
Author(s):  
Hasmukh R. Khunt ◽  
Piyush P. Pipaliya ◽  
Satish M. Ghelani ◽  
Jayesh S. Babariya ◽  
Yogesh T. Naliapara
Keyword(s):  
Antimicrobial Activity ◽  
Antimicrobial Agent ◽  
Synthesis And Characterization ◽  
Bacterial Strains ◽  
Fungal Strains ◽  
Ketene Dithioacetals ◽  
Guanidine Nitrate ◽  
Ketene Dithioacetal

Various ketene dithioacetals of acetoacetanilides were reacted with guanidine nitrate in the presence of base to produce the 2-amino-4-isopropyl-6-alkoxy-N-arylpyrimidine-5-carboxamide derivatives with good yields. All the synthesized compounds were characterized by mass, NMR and IR and also evaluated for antimicrobial activity against five different bacterial and fungal strains. The compounds 4i, 4k and 4l has found comparatively good active against all the bacterial strains.

scholarly journals Molecular Characterization of Brevibacillus laterosporus and Its Potential Use in Biological Control

2004 ◽  
Vol 70 (11) ◽  
pp. 6657-6664 ◽  
Author(s):  
Edmar Justo de Oliveira ◽  
Leon Rabinovitch ◽  
Rose Gomes Monnerat ◽  
Liana Konovaloff Jannotti Passos ◽  
Viviane Zahner
Keyword(s):  
Dna Analysis ◽  
Biomphalaria Glabrata ◽  
Molecular Techniques ◽  
Anticarsia Gemmatalis ◽  
Anthonomus Grandis ◽  
Bacterial Strains ◽  
Isolation And Identification ◽  
Pathogenic Potential ◽  
Brevibacillus Laterosporus

ABSTRACT Thirty-three strains of Brevibacillus laterosporus, including three novel strains isolated from Brazilian soil samples, were examined for genetic variability by the use of different PCR-based methods. Molecular markers that could characterize bacterial strains with regards to their pathogenic potential were investigated. In addition, toxicity was assessed by the use of insects belonging to the orders Lepidoptera and Coleoptera and the mollusk Biomphalaria glabrata. Among the targets tested, Biomphalaria glabrata demonstrated the highest degree of sensitivity to B. laterosporus, with some strains inducing 90 to 100% mortality in snails aged 3 and 12 days posteclosion. Larvae of the coleopteron Anthonomus grandis were also susceptible, presenting mortality levels of between 33 and 63%. Toxicity was also noted towards the lepidopteron Anticarsia gemmatalis. In contrast, no mortality was recorded among test populations of Tenebrio molitor or Spodoptera frugiperda. The application of intergenic transcribed spacer PCR and BOX-PCR generated 15 and 17 different genotypes, respectively. None of the molecular techniques allowed the identification of a convenient marker that was associated with any entomopathogenic phenotype. However, a 1,078-bp amplicon was detected for all strains of B. laterosporus when a primer for amplification of the BOXA1R region was used. Similarly, a 900-bp amplicon was generated from all isolates by use of the primer OPA-11 for randomly amplified polymorphic DNA analysis. These amplicons were not detected for other phenotypically related Brevibacillus species, indicating that they represent markers that are specific for B. laterosporus, which may prove useful for the isolation and identification of new strains of this species.

scholarly journals Production and Characterization of Biomass Briquettes from Tannery Solid Waste

Recycling ◽  
2017 ◽  
Vol 2 (4) ◽  
pp. 17 ◽  
Author(s):  
Imeh Onukak ◽  
Ibrahim Mohammed-Dabo ◽  
Alewo Ameh ◽  
Stanley Okoduwa ◽  
Opeoluwa Fasanya
Keyword(s):  
Solid Waste ◽  
Tannery Solid Waste

Physico-chemical analysis of tannery solid waste and structural characterization of its isolated humic acids after composting

2008 ◽  
Vol 160 (2-3) ◽  
pp. 448-455 ◽  
Author(s):  
Soumia Amir ◽  
Fatima Benlboukht ◽  
Nadia Cancian ◽  
Peter Winterton ◽  
Mohamed Hafidi
Keyword(s):  
Chemical Analysis ◽  
Solid Waste ◽  
Humic Acids ◽  
Structural Characterization ◽  
Physico Chemical ◽  
Tannery Solid Waste

scholarly journals Synthesis and Characterization of Substituted Bisbenzoxazine as Potent Antimicrobial Agent

2015 ◽  
Vol 43 ◽  
pp. 41-48
Author(s):  
Kishor R. Lalcheta ◽  
Bhvin B. Dhaduk
Keyword(s):  
Antimicrobial Activity ◽  
Antimicrobial Agent ◽  
Synthesis And Characterization ◽  
Bacterial Strains ◽  
Fungal Strains

Bisbenoxazines derivatives were synthesized by refluxing substituted bisphenol-C, substituted aniline and formaldehyde in presence of 1, 4-dioxane with good yields. All the synthesized compounds were characterized by mass, NMR and IR and also evaluated for antimicrobial activity against four different bacterial and two fungal strains. The compounds 1c, 1h, 1j and 1l has found comparatively good active against all the bacterial strains.

Extraction and characterization of sea anemones compound and its Anti bacterial and hemolytic studies

2020 ◽  
Vol 10 (4) ◽  
pp. 93-97
Author(s):  
Anil Kumar A ◽  
Raja Sheker K ◽  
Naveen B ◽  
Abhilash G ◽  
Akila CR
Keyword(s):  
Antibacterial Activity ◽  
Focal Point ◽  
Haemolytic Activity ◽  
Klebsiella Pneumonia ◽  
Bacterial Strains ◽  
Sea Anemones ◽  
Marine Plants ◽  
Heteractis Magnifica ◽  
Trididemnum Solidum

Seas assets that give us a variety of characteristic items to control bacterial, contagious and viral ailment and mostly utilized for malignancy chemotherapy practically from spineless creatures, for example, bryozoans, wipes, delicate corals, coelenterates, ocean fans, ocean bunnies, molluscs and echinoderms. In the previous 30 - 40 years, marine plants and creatures have been the focal point of overall endeavours to characterize the regular results of the marine condition. Numerous marine characteristic items have been effectively exceptional to the last phases of clinical preliminaries, including dolastatin-10, a group of peptides disengaged from Indian ocean rabbit, Dollabella auricularia. Ecteinascidin-743 from mangrove tunicate Ecteinascidia turbinata, Didemnins was isolated from Caribbean tunicate Trididemnum solidum and Conopeptides from cone snails (Conus sp.), and a developing number of up-and-comers have been chosen as promising leads for expanded pre-clinical appraisals. Sea anemones possess numerous tentacles containing stinging cells or cnidocytes. The stinging cells are equipped with small organelles known as nematocysts. The two species of sea anemones namely, Heteractis magnificaandStichodactyla haddoni, were collected from Mandapam coastal waters of Ramanathapuram district, Tamilnadu, India. The Nematocyst was collected and centrifuged, and the supernatant was lyophilized and stored for further analysis. The amount of protein from Heteractis Magnifica and Stichodactyla haddoni was estimated. The crude extract has shown haemolytic activity on chicken blood and goat blood. In the antibacterial activity of the sea anemone against six bacterial strains Staphylococcus aureus, Salmonella typhii, Salmonella paratyphii, Klebsiella pneumonia, Vibrio cholerae, Pseudomonas aeruginosa. Antibacterial activity of H. Magnifica and S.haddoni was measured as the radius of the zone of inhibition.

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