Sequence analysis and confirmation of type IV pili-associated proteins PilY1, PilW and PilV in Acidithiobacillus thiooxidans

AbstractAcidithiobacillus thiooxidans is an acidophilic chemolithoautotrophic bacterium widely used in the mining industry due to its metabolic sulfur-oxidizing capability. The biooxidation of sulfide minerals is enhanced through the attachment of A. thiooxidans cells to the mineral surface. The Type IV pili (TfP) of At. thiooxidans may play an important role in the bacteria attachment, since among other functions, TfP play a key adhesive role in the attachment to and colonization of different surfaces. In this work, we reported for the first time the confirmed mRNA sequences of three TfP proteins from At. thiooxidans, the protein PilY1 and the TfP pilins PilW and PilV. The nucleotide sequences of these TfP proteins show changes of some nucleotide positions with respect to the corresponding annotated sequences. The bioinformatic analyses and 3D-modeling of protein structures sustain their classification as TfP proteins, as structural homologs of the corresponding proteins of P. aeruginosa, results that sustain the role of PilY1, PilW and PilV in pili assembly. Also, that PilY1 comprises the conserved Neisseria-PilC (superfamily) domain of the tip-associated adhesin, while PilW of the superfamily of putative TfP assembly proteins and PilV belongs to the superfamily of TfP assembly protein. Also, the analyses suggested the presence of specific functional domains involved in adhesion, energy transduction and signaling functions. The phylogenetic analysis indicated that the PilY1 of Acidithiobacillus genus forms a cohesive group linked with iron- and/or sulfur-oxidizing microorganisms from acid mine drainage or mine tailings. This work enriches knowledge regarding colonization, adhesion and biooxidation of inorganic sulfurs by A. thiooxidans.

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Sequence analysis and confirmation of the type IV pili-associated proteins PilY1, PilW and PilV in Acidithiobacillus thiooxidans

PLoS ONE ◽

10.1371/journal.pone.0199854 ◽

2019 ◽

Vol 14 (1) ◽

pp. e0199854

Author(s):

Elvia Alfaro-Saldaña ◽

Araceli Hernández-Sánchez ◽

O. Araceli Patrón-Soberano ◽

Marizel Astello-García ◽

J. Alfredo Méndez-Cabañas ◽

...

Keyword(s):

Sequence Analysis ◽

Type Iv Pili ◽

Acidithiobacillus Thiooxidans ◽

Type Iv ◽

Associated Proteins

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Changes in the expression of genes encoding type IV pili-associated proteins are seen when Clostridium perfringens is grown in liquid or on surfaces

BMC Genomics ◽

10.1186/s12864-020-6453-z ◽

2020 ◽

Vol 21 (1) ◽

Cited By ~ 1

Author(s):

Samantha R. Soncini ◽

Andrea H. Hartman ◽

Tara M. Gallagher ◽

Gary J. Camper ◽

Roderick V. Jensen ◽

...

Keyword(s):

Clostridium Perfringens ◽

Type Iv Pili ◽

Type Iv ◽

Expression Of Genes ◽

Genes Encoding ◽

Associated Proteins

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Electronic Waste Management by Biological Leaching

Bulletin of Scientific Research ◽

10.34256/bsr1918 ◽

2019 ◽

Vol 1 (1) ◽

pp. 54-61

Author(s):

Gokul V ◽

Rajasekaran T

Keyword(s):

Acid Medium ◽

Manufacturing Industry ◽

Mine Drainage ◽

Electronic Waste ◽

Mining Industry ◽

Negative Consequences ◽

Policy Makers ◽

Acidophilic Bacteria ◽

Sulfur Oxidizing ◽

Mining Water

The electronic industry is the largest and fastest growing manufacturing industry in the world. Electronic waste or E-waste is one of the emerging problems in developed and developing countries. Most of these e-wastes are ending up in dumping yards and recycling centers, posing a new challenge to the environment and policy makers as well. Toxic metals in the E-waste are usually non -biodegradable and they will create harmful long lasting negative consequences on the environment in general and our health particular. Currently E-waste is treated by chemical leaching, it gives more environmental issues due to usage of inorganic chemicals in treatment process. So that, the E-waste can be used for extraction of metals by the process of biological leaching. It is the process of extraction of metal from source by using biological organism. In this process involve numerous ferrous iron and sulfur oxidizing bacteria, acidophilic bacteria which is grown in acid medium. The acid mine drainage (AMD) is the outflow of acidic water from metal mines or coal mines. AMD is recognized as one of the most serious environmental problem in the mining industry. It is also toxic and difficult to managing this water. Due to the presence of sulphide metal in the ore, which is released after the mining process, will react with atmosphere and water forms sulphuric acid which poses potential harm to the environment and eco system. This acid medium is most suitable for the development of acidophilic bacteria’s. In this project, the Acidophilic bacteria in AMD can be suggested for process of recycling in biological leaching of electronic waste. This process can be used for both bioleaching of E-waste and management of mining waste water. The process will suppress the harmfulness in both E-waste and waste mining water.

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Biofilm Formation Is Crucial for Efficient Copper Bioleaching From Bornite Under Mesophilic Conditions: Unveiling the Lifestyle and Catalytic Role of Sulfur-Oxidizing Bacteria

Frontiers in Microbiology ◽

10.3389/fmicb.2021.761997 ◽

2021 ◽

Vol 12 ◽

Author(s):

Roberto A. Bobadilla-Fazzini ◽

Ignacio Poblete-Castro

Keyword(s):

Biofilm Formation ◽

Mining Industry ◽

Copper Recovery ◽

Acidithiobacillus Thiooxidans ◽

Cu Content ◽

Planktonic Form ◽

A Cell ◽

Catalytic Role ◽

Sulfur Oxidizing

Biofilm formation within the process of bioleaching of copper sulfides is a relevant aspect of iron- and sulfur-oxidizing acidophilic microorganisms as it represents their lifestyle in the actual heap/dump mining industry. Here, we used biofilm flow cell chambers to establish laminar regimes and compare them with turbulent conditions to evaluate biofilm formation and mineralogic dynamics through QEMSCAN and SEM-EDS during bioleaching of primary copper sulfide minerals at 30°C. We found that laminar regimes triggered the buildup of biofilm using Leptospirillum spp. and Acidithiobacillus thiooxidans (inoculation ratio 3:1) at a cell concentration of 106 cells/g mineral on bornite (Cu5FeS4) but not for chalcopyrite (CuFeS2). Conversely, biofilm did not occur on any of the tested minerals under turbulent conditions. Inoculating the bacterial community with ferric iron (Fe3+) under shaking conditions resulted in rapid copper recovery from bornite, leaching 40% of the Cu content after 10 days of cultivation. The addition of ferrous iron (Fe2+) instead promoted Cu recovery of 30% at day 48, clearly delaying the leaching process. More efficiently, the biofilm-forming laminar regime almost doubled the leached copper amount (54%) after 32 days. In-depth inspection of the microbiologic dynamics showed that bacteria developing biofilm on the surface of bornite corresponded mainly to At. Thiooxidans, while Leptospirillum spp. were detected in planktonic form, highlighting the role of biofilm buildup as a means for the bioleaching of primary sulfides. We finally propose a mechanism for bornite bioleaching during biofilm formation where sulfur regeneration to sulfuric acid by the sulfur-oxidizing microorganisms is crucial to prevent iron precipitation for efficient copper recovery.

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Analysis of professional incidence of workers of the mining industry of the Orenburg region

Russian Journal of Occupational Health and Industrial Ecology ◽

10.31089/1026-9428-2019-59-9-650-651 ◽

2020 ◽

pp. 650-650

Author(s):

B. L. Kolesnikov ◽

E. M. Egorova ◽

O. S. Redina

Keyword(s):

Occupational Diseases ◽

Mining Industry ◽

Orenburg Region ◽

Clinical Hospital ◽

Regional Center ◽

Mining Enterprise ◽

First Time

The analysis for the first time of the established occupational diseases to employees of the mining enterprise of the Orenburg region in the regional center of professional pathology of Orenburg Regional Clinical Hospital during 2016-2018 is carried out.

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