scholarly journals Seroprevalence of human Brucellosis and associated risk factors among high risk occupations in Mbeya Region of Tanzania

2019 ◽  
Author(s):  
Frederick D. Sagamiko ◽  
John B. Muma ◽  
Esron D. Karimuribo ◽  
Alfred A. Mwanza ◽  
Ruth L. Mfune ◽  
...  
Keyword(s):  
Public Health ◽  
Risk Factors ◽  
Developing Countries ◽  
High Risk ◽  
Control Measures ◽  
Economic Losses ◽  
Enzyme Linked Immunosorbent Assay ◽  
Human Brucellosis ◽  
Associated Risk Factors ◽  
Abattoir Workers

AbstractBackgroundBrucellosis is an infectious zoonotic disease that affects humans, livestock and wildlife.MethodsA cross-sectional study was conducted in Mbeya region between November 2015 and January 2016 to investigate the seroprevalence of human brucellosis and identify associated risk factors among individuals in risky occupations in Mbeya Region. A total of 425 humans from six occupational categories were serially tested for Brucella antibodies using the Rose Bengal Plate Test (RBPT) and competitive Enzyme Linked Immunosorbent Assay (c-ELISA), for screening and confirmation, respectively. A questionnaire survey was administered to participants collect epidemiological data.ResultsThe overall seroprevalence among the high risk occupational individuals was 1.41% (95% CI: 0.01-0.03). Seroprevalence among the different occupations were as follows: shepherds 1.33% (95% CI: 0.14-0.22); butcher men 5.26% (95% CI: 0.10-0.17) and abattoir workers 1.08% (95% CI: 0.39-0.49). Seroprevalence was noted to vary according to occupation type, milk consumption behaviour, age and sex. Butcher men recorded the highest seroprevalence (5.0%) while individuals who consumed unboiled milk had a higher seroprevalence (1.56%) compared to those who drunk boiled milk. High seropositivity (2.25%) was observed among the age group of 1-10 years while male individuals had a higher seroprevalence (1.41%) than females (0%). Butcher men were at higher risk of exposure compared to other professions.ConclusionOur findings show the presence of brucellosis in occupationally exposed individuals in Mbeya region. There is need to sensitize the exposed individuals in order to reduce the risk of acquiring Brucella infections from animals and animal products This also calls for public health awareness about the disease, and implementation of control measures that will prevent further spread of brucellosis within and outside the study area..Author summaryBrucellosis is a bacterial zoonosis that has evolved to establish itself as an occupational and food-borne disease Worldwide. It is responsible for huge economic losses incurred by livestock keepers and poses a public health risk to humans in most developing countries. In Tanzania, which has the 3rd highest cattle population in Africa, many studies that have been done show that brucellosis exists in livestock, especially in cattle and wildlife. However, very few studies have reported on human brucellosis. The disease has been reported to occur in humans who have direct exposure to cattle or cattle products like livestock farmers, abattoir workers, veterinarians, shepherds and farm workers in many developing countries. A few studies in Tanzania have reported seroprevalences among these high-risk occupations; however, the disease has not been fully described in Mbeya region. This study was therefore aimed at filling these information gaps and contributing to the existing body of knowledge.

Seroprevalence of contagious bovine pleuropneumonia in cattle of Punjab, Pakistan and assessment of risk factors

2019 ◽  
Author(s):  
Ahsan Anjum ◽  
Asim Aslam ◽  
Raheela Akhtar ◽  
Tahir Yaqub ◽  
Junaid Naseer ◽  
...  
Keyword(s):  
Risk Factors ◽  
Cross Sectional Study ◽  
Control Measures ◽  
Export Market ◽  
Economic Losses ◽  
Enzyme Linked Immunosorbent Assay ◽  
Contagious Bovine Pleuropneumonia ◽  
Serum Samples ◽  
Cross Sectional ◽  
Associated Risk Factors

Contagious bovine pleuropneumonia (CBPP) is an important infectious disease of respiratory system of cattle, caused by Mycoplasma mycoides subspecies mycoides. A cross sectional study was conducted in Lahore, Kasur and Jhang districts of Punjab to determine the prevalence of CBPP. A total of 560 serum samples were examined for the specific antibodies against M. mycoides subspecies mycoides, using a sandwich enzyme-linked immunosorbent assay. Associated risk factors that were evaluated include age, gender and breed. In this study, the overall seroprevalence was 8.75%. The seroprevalence of CBPP in Lahore, Kasur and Jhang were 8.26, 8.20 and 10.06%, respectively, but no significance difference (P less than 0.05) was observed. Among the assessed potential predisposing factors, gender and breed were not significantly associated with the serological status of the animal (P greater than 0.05), in which the age affected significantly the positivity to CBPP (P less than 0.05). The highest seropositivity was observed in animals of more than 7 years of age (32.08%) followed by in 4-7 years of age (8.16%) and lowest in animals of less than 4 years old (0.79%). This study confirms the presence of CBPP in the state which could be a threat to livestock export market and warrants the implementation of control measures to mitigate the economic losses associated with the disease. 

scholarly journals Seroprevalence of human Brucellosis and associated risk factors among high-risk occupations in Mbeya Region of Tanzania

2020 ◽  
Vol 6 (1) ◽  
pp. 1 ◽  
Author(s):  
Frederick D. Sagamiko ◽  
Ruth L. Mfune ◽  
Bernard M. Hang’ombe ◽  
Esron D. Karimuribo ◽  
Alfred M. Mwanza ◽  
...  
Keyword(s):  
Public Health ◽  
Risk Factors ◽  
Statistical Significance ◽  
Raw Milk ◽  
Enzyme Linked Immunosorbent Assay ◽  
Human Brucellosis ◽  
P Value ◽  
Cross Sectional ◽  
Occupationally Exposed ◽  
Associated Risk Factors

Background: Brucellosis is a bacterial zoonotic disease of public health and economic importance. A cross-sectional study was conducted in Mbeya region between November 2015 and January 2016 to investigate the seroprevalence of human brucellosis and identify associated risk factors among individuals in risky occupations. Methods: A total of 425 humans from six occupational categories were serially tested for Brucella antibodies using the Rose Bengal Plate Test (RBPT) and competitive Enzyme Linked Immunosorbent Assay (c-ELISA), for screening and confirmation, respectively. A questionnaire survey was also administered to participants to collect epidemiological data. Results: The overall seroprevalence among the occupationally exposed individuals was 1.41% (95% CI: 00.64-3.12). Seroprevalence was higher among butcher men 5.6% (95% CI: 1.68-5.26), herds men 1.35% (95% CI: 0.18-9.02); and abattoir workers 1.1% (95% CI: 0.26-4.22) although there was no statistical significance. (P value = .18). Seroprevalence was also higher among men (1.8%) compared to females (0%) (P value = .19). and also, among those aged < 11 years (2.5%). Individuals who consumed raw milk had a higher seroprevalence (1.56%) compared to those who drunk boiled milk while seropositivity was 0.88% among those who assisted animals during parturition (P value = .49). Butcher men were at higher risk of exposure compared to other occupational categories. Our findings show the presence of brucellosis in occupationally exposed individuals in Mbeya region.Conclusion: There is need to sensitize the concerned professions in order to reduce the risk of acquiring Brucella infections from animals and animal products This also calls for public health awareness about the disease, and implementation of measures to prevent further spread of brucellosis within and outside the study area.

scholarly journals Seroprevalence of Toxoplasma gondii and associated risk factors in domestic pigs raised from Cuba

2021 ◽  
Author(s):  
Julio César Castillo-Cuenca ◽  
Álvaro Martínez-Moreno ◽  
José Manuel Diaz-Cao ◽  
Angel Entrena-García ◽  
Jorge Fraga ◽  
...  
Keyword(s):  
Risk Factors ◽  
Toxoplasma Gondii ◽  
Meat Products ◽  
Control Measures ◽  
Health Concern ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Cross Sectional ◽  
Weaning Pigs ◽  
Associated Risk Factors

AbstractA cross-sectional study was carried out to determine the seroprevalence of Toxoplasma gondii and associated risk factors in pigs in the largest pork-producing region in Cuba. Serum samples from 420 pigs, including 210 sows and 210 post-weaning pigs, were tested for antibodies against T. gondii using a commercial indirect enzyme-linked immunosorbent assay. Anti-T. gondii antibodies were detected in 56 animals (13.3%, 95% CI: 10.1–16.6). A generalized estimating equations model revealed that the risk factors associated with higher seropositivity in pigs were altitude (higher in farm’s location < 250 m above sea level (masl) versus ≥ 250 masl) and age (higher in sows compared to post-weaning pigs). The results indicated that this protozoan parasite is widely distributed on pig farms in the study area, which is a public health concern since the consumption of raw or undercooked pork meat products containing tissue cysts is considered one of the main routes of T. gondii transmission worldwide. Control measures should be implemented to reduce the risk of exposure to T. gondii in pigs in Cuba.

Sero - Prevalence and Risk Factors of Cattle and Human Brucellosis at Seka Chokorsa and Shebe Sonbo Jimma Zone Districts, South West Ethiopia

2019 ◽  
Author(s):  
Ftsum Assefa Tokon ◽  
Benti Deresa Gelalcha ◽  
Teferi Benti Moti ◽  
Redeat Belaneh Alemu ◽  
Hailu Degefu Awash
Keyword(s):  
Public Health ◽  
Risk Factors ◽  
Brucella Abortus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Human Brucellosis ◽  
Bacterial Disease ◽  
Serum Samples ◽  
Public Health Importance ◽  
Herd Level ◽  
Jimma Zone

Abstract Background: Brucellosis is contagious bacterial disease of major socio-economic and public health importance globally and it is also one of the priority zoonotic diseases in Ethiopia. Across-sectional epidemiological study was carried out from April 2017 to April 2018 to estimate the sero prevalence of bovine and human brucellosis and to assess the associated risk factors of brucellosis in Seka Chokorsa and Shebe Sonbo districts of Jimma zone. Results: The overall prevalence of cattle brucella infection at individual and herd level were 5.9% (95%CI: 4.1%-8.1%) and 26.6 (95%CI: 19.1%-35.3%) based on diagnosis using commercial kits of the competitive enzyme-linked Immunosorbent assay (CELISA) from Brucella abortus antibodies. Univariate logistic regression analysis at individual animal level showed that animals from large herd size and households which had a practice of introduction of new animals in their herds were 3.7 and 2.3 times more likely to be seropositive, respectively. The same scenario has been observed at herd level. Molecularly, five Brucella abortus was identified as the species affecting cattle in the study areas. From the total human serum samples tested only one serum was found to be positive for CFT and thus the prevalence is 0.42 %,( 95%CI: 0.01%-2.38%). From 238 respondents, 90% of them drink raw milk and milk products, and 70% of them also eat raw meat. Furthermore, slaughtering, assisting during delivery and poor management of aborted material is a common practice that favor transmission of the pathogen in the area. Conclusions: In conclusion, the results of this study showed that brucellosis is an important and widely distributed disease in cattle in the study areas. The finding of the infection in human indicates the public health importance of brucellosis in the area. The risk behaviors and practices observed suggests the need to design all-inclusive health programmes, such one-health approach aimed at controlling brucellosis spread in the study area.

scholarly journals Seroprevalence and Risk Factors Associated with Contagious Caprine Pleuropneumonia in Western Amhara, Northwest Ethiopia

2019 ◽  
Vol 2019 ◽  
pp. 1-7
Author(s):  
Askale Abrhaley ◽  
Mebrat Ejo ◽  
Tsegaw Fentie
Keyword(s):  
Risk Factors ◽  
Cross Sectional Study ◽  
Northwest Ethiopia ◽  
Control Measures ◽  
Enzyme Linked Immunosorbent Assay ◽  
Multivariable Logistic Regression Analysis ◽  
High Seroprevalence ◽  
Cross Sectional ◽  
Contagious Caprine Pleuropneumonia ◽  
Associated Risk Factors

Contagious caprine pleuropneumonia (CCPP) has been identified as a significant problem in goat production, especially in the arid and semiarid lowland areas of Ethiopia. Even though CCPP was reported in most of the goat rearing areas of the country, there is no adequate information on the disease in the Amhara Region. Cross-sectional study was conducted from November 2016 to April 2017 in the districts of Western Amhara to estimate the seroprevalence and identify the associated risk factors for occurrence of the CCPP. The risk factors considered included age, sex, agroclimate, and districts. A competitive enzyme-linked immunosorbent assay (cELISA) was carried out on a total of 400 goat sera samples, out of which 34 samples were found seropositive for specific antibodies against CCPP, with the overall seroprevalence of 8.5% (95% confidence interval (CI) =5.8, 11.2). Among the epidemiological factors considered, age and sex of the goats were not significantly associated with CCPP seroprevalence (p>0.05). However, the seropositivity was slightly higher in adults (9.9%) and female goats (9.0%) compared to young (6.3%) and male (7.5%) goats, respectively. The analysis of seroprevalence by district shows that the seroprevalence of CCPP in Metema (OR=14.34; 95%CI= 1.80, 114.09; p=0.012) and Fogera (OR=9.99; 95%CI= 1.10, 91.16; p= 0.041) was significantly higher compared to other study districts. Multivariable logistic regression analysis also identified the district as a risk factor for the occurrence of a high seroprevalence of CCPP. The present study revealed the seroprevalence and the distribution of CCPP in Western Amhara districts, and hence appropriate control measures including regular investigation and vaccination should be implemented to alleviate the problem.

scholarly journals Seroprevalence and Associated Risk Factors of Brucellosis in Livestock and Residents of New Valley Governorate, Egypt

2020 ◽  
pp. 531-539
Author(s):  
Mohamed Said Diab ◽  
Sherif Abd Allah Zidan ◽  
Nermin Abdalla A. Hassan ◽  
Haitham Elaadli ◽  
Ahmed Mohamed Bayoumi
Keyword(s):  
Risk Factors ◽  
Raw Milk ◽  
Cross Sectional Study ◽  
Enzyme Linked Immunosorbent Assay ◽  
Human Brucellosis ◽  
Human Occupation ◽  
Serum Samples ◽  
Cross Sectional ◽  
Significant Difference ◽  
Associated Risk Factors

Brucellosis is a worldwide zoonotic disease which is now considered endemic in most parts of Egypt. A cross-sectional study was carried out from December 2018 to February 2020 to investigate the seroprevalence of brucellosis in humans and livestock residing in two regions located in New Valley Governorate, Egypt. A total of 1254 animals (673 cattle, 348 sheep, and 233 goats) and 523 human serum samples were examined for brucellosis using Rose Bengal test (RBT) and then randomly selected sera (15 from cattle, 7 from sheep, 3 from goats, and 45 from humans) were further analyzed by complement fixation test, enzyme-linked immunosorbent assay to compare and detect the sensitivity and specificity of RBT. The prevalence of brucellosis was 0% in cattle, sheep, and goats while it was 23.9% in humans using RBT. Concerning humans, there was a higher percentage of infection in EL Kharga (33.6%). The prevalence of this infection was also at a higher level among individuals aged above 40 years (28.57%). Furthermore, men (26.11%) were more inclined to be inflicted, compared to women (22.5%) with no significant difference. Considering the human occupation, abattoir workers were the most predominant group of people at risk (33.3%), followed by farmers (31.25%) and animal keepers (20.6%) while the lowest prevalence was demonstrated in the housewives where the prevalence was 18.8 %. As a result, risk factors of the age range, locality, time of infection, contact with animals, and occupational groups could significantly affect the prevalence of human brucellosis in the New Valley Governorate. In conclusion, brucellosis is an alarming problem among residents of the New Valley Governorate. Thus, reducing the prevalence in humans and animals in the region of study may include restriction of the marketing the raw milk and enhancing public health awareness.

scholarly journals Epidemiology and Associated Risk Factors of Hepatitis E Virus Infection in Plateau State, Nigeria

2014 ◽  
Vol 5 ◽  
pp. VRT.S15422 ◽  
Author(s):  
Surajudeen A. Junaid ◽  
Samuel E. Agina ◽  
Khadijah A. Abubakar
Keyword(s):  
Public Health ◽  
Risk Factors ◽  
Pregnant Women ◽  
Waste Disposal ◽  
Hepatitis E Virus ◽  
Public Health Problem ◽  
Hepatitis E ◽  
Enzyme Linked Immunosorbent Assay ◽  
Associated Risk Factors ◽  
Animal Handlers

A cross-sectional study in Nigeria was undertaken to determine the epidemiology, seroprevalence, and associated risk factors, of hepatitis E virus (HEV). A total of 462 subjects were used for the study, categorized into four groups: apparently healthy persons, pregnant women, HIV positive subjects, and animal handlers. Information was obtained from subjects using interviewer-administered questionnaire. Blood samples were collected and analyzed for HEV antibodies (IgG and IgM) using enzyme-linked immunosorbent assay (ELISA) technique. Results obtained were analyzed using Statistical Package for Social Sciences (SPSS) version 17.0 statistical software. The overall seroprevalence of IgG and IgM was 42.7 and 0.9%, respectively. Animal handlers had the highest seroprevalence (66.7%). The associated risk factors for IgM seroprevalence were rural dwelling ( P = 0.039, odds ratio (OR) 3.3, 95% confidence interval (CI) 0.7–15.4), blood transfusion ( P < 0.001, OR 9.6, 95% CI 2.6–35.6), attending to animals ( P = 0.032, OR 4.9, 95% CI 0.9–26.6), and waste disposal ( P < 0.001). Factors associated with IgG were age ( P = 0.044), location ( P < 0.001), marital status ( P < 0.001), formal education ( P < 0.001), farming as occupation ( P < 0.001), rural dwelling ( P = 0.001), waste disposal ( P < 0.001), alcohol consumption ( P = 0.001, OR 2.4, 95% CI 1.4–4.0), open defecation ( P < 0.001, OR 2.9, 95% CI 1.4–5.7), attending to animals ( P < 0.001, OR 2.3, 95% CI 1.6–3.4), consuming unwashed fruits/vegetables ( P < 0.001, OR 4.2, 95% CI 0.3–54.1), and stream/river as a source of drinking water ( P < 0.001, OR 3.6, 95% CI 1.6–7.8). Preventive public health measures should be reinforced among all communities, particularly domestic animal handlers and pregnant women. Potable water should be provided for all communities. Data suggest that HEV remains an under-recognized and significant public health problem, warranting further attention and research.

scholarly journals Seroprevalence and Associated Risk Factors of Bovine Brucellosis in District Gujranwala, Punjab, Pakistan

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1744
Author(s):  
Muhammad Rashid Khan ◽  
Abdul Rehman ◽  
Salman Khalid ◽  
Mansur Ud Din Ahmad ◽  
Muhammad Avais ◽  
...  
Keyword(s):  
Risk Factors ◽  
Herd Size ◽  
Cross Sectional Study ◽  
Economic Losses ◽  
Enzyme Linked Immunosorbent Assay ◽  
Bovine Brucellosis ◽  
Cross Sectional ◽  
History Of ◽  
Awareness Programs ◽  
Associated Risk Factors

Bovine brucellosis is a contagious zoonotic disease that causes economic losses through abortion and infertility. A cross-sectional study was designed to determine the seroprevalence and associated risk factors of bovine brucellosis in district Gujranwala of Punjab, Pakistan. A total of 220 bovine sera (112 from buffaloes, 108 from cattle) from 46 unvaccinated herds were collected. Parallel testing by the Rose Bengal Plate Test (RBPT) and Indirect Enzyme-linked Immunosorbent Assay (I-ELISA) showed a 58.7% (27/46) herd-level and 22.7% (50/220) animal-level seroprevalence. Seroprevalence was higher (p < 0.001, OR = 7.62) in adult animals (37.2%) compared to younger animals (4.9%). A herd size of >10 animals (p = 0.021, OR = 7.83), less housing space (p = 0.037, OR = 6.39) and history of abortion at the farm (p = 0.023, OR = 5.6) were found as risk factors associated with the seropositivity of brucellosis. There was a substantial agreement between the RBPT and I-ELISA results (Cohen’s kappa coefficient (κ) = 64.16, percent agreement = 89.5%). In conclusion, a relatively higher seroprevalence was found compared to the previous reports from the country. Standardization and validation of the advanced diagnostic tests would be needed. Biosecurity, personal protection, quarantine measures and routine screening of animals at the farm level and disease awareness programs and consumption of pasteurized milk in the human population will be helpful in preventing the transmission/zoonosis of the disease.

scholarly journals Seroprevalence and Risk Factors Associated with Canine Leishmaniasis in Egypt

2021 ◽  
Vol 8 (10) ◽  
pp. 236
Author(s):  
Abdelfattah Selim ◽  
Salma Shoulah ◽  
Abdelhamed Abdelhady ◽  
Abdulaziz Alouffi ◽  
Yasser Alraey ◽  
...  
Keyword(s):  
Risk Factors ◽  
Control Measures ◽  
Sand Fly ◽  
Canine Leishmaniasis ◽  
Effective Control ◽  
Enzyme Linked Immunosorbent Assay ◽  
Seroprevalence Rate ◽  
Serum Samples ◽  
Group 2 ◽  
Associated Risk Factors

Background: Canine leishmaniasis (CanL) is caused by Leishmania infantum (L. infantum) that is transmitted by sand fly vectors with dogs acting as the main reservoir. Methods: The present study aimed to determine the seroprevalence of CanL in dogs from Egypt and assessed the associated risk factors. The study was conducted from 2019 to 2020 in five governorates situated in Northern Egypt. Serum samples from 450 asymptomatic dogs were serologically examined by use of enzyme-linked immunosorbent assay (ELISA). Results: Overall, the seroprevalence rate of CanL was 21.3% and the highest rates were observed in Cairo and Giza governorates. The univariable analysis revealed that the seropositivity of CanL was strongly related to the dogs′ ages, length of hair, absence of veterinary care or application of insecticides, and the type of floor of their shelters. The risk factors that were found to be associated with CanL in exposed dogs were: age group 2–4 years old (OR = 12, 95% CI: 1.6–92.3); short hair (OR = 2.07, 95% CI: 1.2–3.6); absence of veterinary care (OR = 2.7, 95% CI: 1.3–5.8); no application of insecticides (OR = 3.09, 95% CI: 1.5–6.5) and their residence in a shelter with an earthen floor (OR = 1.42, 95% CI: 0.7–2.9). Conclusions: Based on the present results, CanL is present in Egyptian dogs and this increases the possibility of transmission by sand fly to humans with whom they have contact. Consequently, an efficient monitoring programme and effective control measures are important to reduce the risk of infection.

scholarly journals Contagious bovine pleuropneumonia: Seroprevalence and risk factors in Western Oromia, Ethiopia

2016 ◽  
Vol 83 (1) ◽  
Author(s):  
Garuma Daniel ◽  
Mukarim Abdurahaman ◽  
Getachew Tuli ◽  
Benti Deresa
Keyword(s):  
Risk Factors ◽  
Body Condition ◽  
Agricultural Research ◽  
Geographical Location ◽  
Control Measures ◽  
Economic Losses ◽  
Enzyme Linked Immunosorbent Assay ◽  
Contagious Bovine Pleuropneumonia ◽  
Potential Risk Factors ◽  
Small Colony

Contagious bovine pleuropneumonia (CBPP) is one of the most important threats to cattle health and production in Ethiopia. At the livestock farm of the Bako Agricultural Research Center, an outbreak of respiratory disease of cattle occurred in May 2011, and many animals were affected and died before the disease was diagnosed. Therefore, this study was designed to determine the seroprevalence of CBPP antibodies in selected districts of Western Oromia Region and to assess the potential risk factors for the occurrence of the disease. A crosssectional study was conducted from November 2013 to March 2014 in three selected districts of Western Oromia Region. A total of 386 sera were examined for the presence of specific antibodies against Mycoplasma mycoidesmycoides small colony (MmmSC), using a competitive enzyme-linked immunosorbent assay. The risk factors that were evaluated in this study were geographical location, age, sex, breed and body condition. The overall seroprevalence in this study was 28.5%. The seroprevalence of Mycoplasma mycoidesmycoides small colony antibodies at the district level was 40.3%, 19.0% and 5.7% in Gobbu Sayyo, BakoTibbe and Horro districts, respectively. There was a statistically significant variation ( p < 0.05) in the prevalence of antibodies amongst the districts. However, animal-related risk factors, such as age, sex, breed and body condition, were not significantly associated ( p > 0.05) with the serological status of the animal. This study showed that the overall prevalence of CBPP in Western Oromia Zones was high. This warrants the implementation of appropriate preventive and control measures to minimise the economic losses associated with the disease.Keywords: Seroprevalence, CBPP, risk factors, c-ELISA, Western Oromia Zones, Ethiopia

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